Animal Development PreLab
1 Page

Animal Development PreLab

Course Number: BIOSCI 0060, Spring 2007

College/University: Pittsburgh

Word Count: 276

Rating:

Document Preview

Lauren Allen Monday at 6 Due April 2, 2007 Animal Development Pre-lab Briefly explain cloning There are several ways to clone: 1. Make multiple copies of a DNA sequence 2. To clone an animal (like Dolly) from a clone cell. Cloning DNA sequences To clone you need a set of enzymes called restriction endonucleases. They are enzymes that are able to fragment DNA at certain restriction sites. These endonucleases cut...

Unformatted Document Excerpt
Coursehero >> Pennsylvania >> Pittsburgh >> BIOSCI 0060

Course Hero has millions of student submitted documents similar to the one
below including study guides, practice problems, reference materials, practice exams, textbook help and tutor support.

Course Hero has millions of student submitted documents similar to the one below including study guides, practice problems, reference materials, practice exams, textbook help and tutor support.

Allen Lauren Monday at 6 Due April 2, 2007 Animal Development Pre-lab Briefly explain cloning There are several ways to clone: 1. Make multiple copies of a DNA sequence 2. To clone an animal (like Dolly) from a clone cell. Cloning DNA sequences To clone you need a set of enzymes called restriction endonucleases. They are enzymes that are able to fragment DNA at certain restriction sites. These endonucleases cut the DNA which have sticky ends and used to be then inserted into bacterial plasmids and then cloned when the bacteria reproduced. From these, you can make a clone library. Cloning Animals When Dolly, the sheep was cloned, they took a mammary cell out of one of her "parents" and grew it in a solution that held up the cell cycle and a nucleus was removed from an egg cell with a micropipette. The mammary cell was inserted then inside the covering of an egg cell. Electric shock opens cell membranes and triggers cell division. The embryo then begins to develop in vitro and is implanted into the surrogate mother. The pregnancy was five months long and a lamb that was genetically identical to the sheep from which the mammary cell was extracted from. Why Cloning Fails "Normal development depends on precise genomic imprinting. This chemical reprogramming of the DNA...takes months for sperm and years for eggs." During cloning the reprogramming must take minutes. The reprogramming job is not done nearly as well because of the time difference. "Human cloning will not be practical until scientists figure out how to reprogram a donor nucleus, as occurs in the DNA of sperm or eggs in our bodies." (Raven 7th Edition Biology)

Find millions of documents on Course Hero - Study Guides, Lecture Notes, Reference Materials, Practice Exams and more. Course Hero has millions of course specific materials providing students with the best way to expand their education.

Below is a small sample set of documents:

Georgia Tech - CHEM - 1310
Chemistry Experiment 12Patrick EisenmannA 1 2BCDEFGHIJKLMNThermochemistry and Hess's Law3 A. Determination of DH (NaOH - HCl or NH 3 - HCl) 4 5 Tabulated Raw Data 6 Trial 1 Trial 2 7 ID Base 8 [HCl] (M) 9 [Base] (M)
Pittsburgh - BIOSCI - 0060
Lauren Allen Lab Monday 6-8:50 Ecology Pre-Lab 1. For reference at the end of a document: Angle, Scott. (2006) Do high-nickel leaves shed by the nickel hyperaccumulator Alyssum murale inhibit seed germination of competing plants?. New Phytologist,173
Pittsburgh - BIOSCI - 0060
Lauren Allen February 12, 2007 Dr. Tom Seiflein Molecular Biology Lab I & IIDetermination of a Bacterial Plasmid via Phenotypic and Genotypic Tests RESULTSTable 1: Expected and Observed Phenotypes of E. Coli with Plasmids Containing Spliced DNA on
Georgia Tech - CHEM - 1310
A 1 2BCDEFGHIJKLMNOThermochemistry and Hess's Law3 A. Determination of DH (NaOH - HCl or NH 3 - HCl) 4 5 Tabulated Raw Data 6 Trial 1 Trial 2 7 ID Base 8 [HCl] (M) 9 [Base] (M) 10 Vol. HCl (mL) 11 Vol. Base (mL) 12 Tim
Rhode Island - BUS - 355
The assignment problem: Flynn Boot Company weekly capacity warehouse Ci atlanta ft. worth tucson total 15000 40000 30000 85000 weekly demand customer Dj billy dude slickers CJ's total 27800 8000 13500 33000 82300cost to ship one pair of boots from
Pittsburgh - BIOSCI - 0060
Lauren Allen March 26, 2007 Dr. Tom Seiflein Territorial Behaviors by Conifers Using AllelochemicalsHYPOTHESESHypothesis for the Rye Seeds: When rye seeds are watered daily for 7 days with one of three conifer leachates or water there will be a si
Georgia Tech - CHEM - 1310
Classes of Chemical ReactionsExcel Template1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60A B C Chem1310 Section: Date of
Pittsburgh - BIOSCI - 0060
Table 1 : Expected and Observed Phenotypes of E. Coli with Plasmids Containing Spliced DNA on Agars Pre-treated with An Plasmid LB only LB / AMP LB / KAN LB / AMP-ARA Expected pAMP + / NO + / NO - / NO + / NO Expected pKAN + / NO - / NO + / NO - / NO
Georgia Tech - CHEM - 1310
Quantum Mechanics - Window to Atomic StructureExcel Template1 2 3 4 5 6A B C Name: Partner's Name: Section: Date of Experiment Performed: Date of Report Prepared:DEF Adam Henderson Matt Dutton L1 2/20/2007 2/26/2007GHIJKLM
Pittsburgh - BIOSCI - 0060
Lauren Allen Lab Monday at 6 PM Mendelian Genetics Pre-Lab 1. A reciprocal cross is a cross in which the female may begin with the dominant genotype and the male with the recessive genotype and then another cross is done where the female is now the r
Pittsburgh - BIOSCI - 0060
Lauren Allen Monday at 6 Due January 22, 2007 Molecular I & II Biology Pre-Lab 1. The organism is E-coli and is a bacterium. 2. A plasmid is a small coiled ring of double-stranded DNA containing accessory genes of a bacterial genome. A plasmid is not
Georgia Tech - CHEM - 1310
Qualitative Analysis of the Group IV and V CationsExcel Template1 2 3 4 5 6 7A B C Chem1310 Name: Partner's Name: Section: Date of Experiment Performed: Date of Report Prepared:DEF Miranda Pryor Cassandra Pryor A4 3/27/2007 4/3/2007GH
Rhode Island - BUS - 355
The assignment problem: Flynn Boot Company weekly capacity Ci 400 500 100 1000 weekly demand Dj 200 250 300 750Plant A B C totalStore X Y Z totalcost to ship one pair of boots from warehouse i to customer j (Tij) X $ $ $ Y $ $ $ Z $ $ $A B C
Pittsburgh - BIOSCI - 0060
Lauren Allen Monday at 6 Due March 26, 2007 Plant Development 1. Coal 2. Autotrophs are organisms (usually plants or bacteria) that can produce their own nutrients. (Some may use sunlight or water) 3. The accessory pigments are used to produce certai
Pittsburgh - BIOSCI - 0060
Lauren Allen Lab Monday 6-8:50 Population Genetics Pre-Lab 1. * work is shown in a separate sheet of paper Parental Generation # animals # alleles 390 140 470 1000 780 280 940 2000Phenotype Smooth Fur Spikey Fur Rough Fur TotalGenotypes SS ss Ss
Pittsburgh - BIOSCI - 0060
Lauren Allen Monday at 6 Due April 2, 2007 Systematics Pre-lab1. Taxonomy is a subdivision of the biological sciences that identifies and names organisms. 2. They were first differentiated by the fact that birds have feathers, reptiles have scales,
Pittsburgh - BIOSCI - 0060
Lauren Allen Lab Monday 6-8:50 Virtual Bacteria Identification Lab 1. These traditional methods are less reliable than the newer methods because it is necessary to begin with a good sample of bacteria. Some bacteria do not grow on solid mediums (some
UCSB - CHEM - 1CL
Time 0 30.77 51.04 71.49 91.83 112.29 132.6 152.97 173.41 193.68 214.12 234.47 254.76 275.24 295.56Absorbance 0.271 0.208 0.177 0.15 0.127 0.107 0.091 0.077 0.066 0.056 0.049 0.042 0.037 0.033 0.03Time 0 30.77 51.04 71.49 91.83 112.29 132.6 152.9
St. Thomas - ACCT - 205
a. Jane Evans invests $5,000 in Evans Consulting, P.C. in return for shares of stock in the corporation. b. Evans Consulting borrows $2,000 from First National Bank. c. Evans consulting buys equipment for $4,000 cash. d. During first month of operati
St. Thomas - ACCT - 205
Financial Accounting 205CH1 Prob1Page 1Demonstration Problem. College student first starting out. Transactions: 1 $15,000 gift from parents 2 Pay $10,000 down for a $100,000 townhouse, finance remainder with mortgage Mortgage payments are $900
Clemson - CE - 311
St. Thomas - ACCT - 205
Practice Problem. College student first starting out. Transactions:YOU GOT 100 % CORRECTATTENTION: Please do not cut/paste at all or copy/paste from outside the worksheet.a. $10,000 gift from parents b. Pay $5,000 down for a $80,000 townhouse, f
St. Thomas - ACCT - 205
Practice Problem. College student first starting out. Transactions:YOU GOT 100 % CORRECTATTENTION: Please do not cut/paste at all or copy/paste from outside the worksheet.a. $10,000 gift from parents b. Pay $5,000 down for a $80,000 townhouse, f
St. Thomas - ACCT - 205
Financial Analysis Project: Best Buy, Inc.Part 1: Company InformationDave JohnsonVideo MenuFrom Yahoo Finance-Profile-Expanded Business Description: Incorporated in Minnesota in 1966 as Sound of Music, Inc., Best Buy Company is a specialty ret
N. Illinois - MUSC - 173
accelerando A2/a due ad libitum adagio agitato alla breve allargando allegretto allegro allmahlich amoroso andante andantino animato/anime/animando a piacere aperto appasionato assai a tempo attacca ausdrucksvoll avec ben/bene bewegt blechblaser brav
St. Thomas - ACCT - 205
The mailroom employees send all remittances and remittance advices to the cashier. The cashier deposits the cash in the bank and forwards the remittance advices and duplicate deposit slips to the accounting department. Indicate the weak link in inter
Pittsburgh - ENGFLM - 0400
Lauren Allen Movie: Imitation of Life Introduction to Film Studies- Tues. 6pm-9:50pmThe Social Event of the Season Extravagant diamonds pour from the sky and fill the opening credits screen. Each jewel floats down and balances on top of a previousl
St. Thomas - ACCT - 205
Chapter 6 Practice ProblemYOU GOT 0 % CORRECTATTENTION: Please do not cut/paste at all or copy/paste from outside the worksheet.750 1,750 1,400 675 4,5751.Purchasing records reveal the following: Balance 1/1/04 1/2 purchase 1/12 purchase 1/25
Tulane - CLAS - 104
Mike Zolnick CLAS-104 #Character Author Chaos Hesiod Aristophanes Ovid Ge/Gaia Hesiod Aristophanes Tartarus Hesiod Aristophanes Eros Hesiod Aristophanes Aristopanes Night Hesiod Aristophanes Day Hesiod Aether Hesiod Uranus HesiodDatesWorkAttr
St. Thomas - ACCT - 205
Which of the following items are properly classified as part of factory overhead for John Deere & Co.? Place "x" if overhead a Plant manager's salary at Greenville, Tennessee turf care products plant. x b Depreciation on Moline Illinois headquarters
St. Thomas - ACCT - 205
Prepaid Accounts Retained Cash expense Supplies = payable earnings b. Paid $2,400 for an insurance premium on a 1-year policy c. Purchased supplies on account, $700 Make the adjusting entries below: Expired insurance for one month Supplies at end of
St. Thomas - ACCT - 205
YOU GOT 0 % CORRECTProblem 1 Record bad debts expense below and the entry to write off an $800 invoice. Estmated Uncollectible Accounts Percent Amount 2.0% $200 5.0% $400 10.0% $600 20.0% $800 80.0% $800 $2,800Age Interval Not past due 1-30 past d
St. Thomas - ACCT - 205
YOU GOT 100 % CORRECTProblem 1 Record bad debts expense below and the entry to write off an $800 invoice. Estmated Uncollectible Accounts Percent Amount 2.0% $200 5.0% $400 10.0% $600 20.0% $800 80.0% $800 $2,800Age Interval Not past due 1-30 past
St. Thomas - ACCT - 205
Accumulated Accounts Prepaid Equip- deprecCash receivable Inventory expense ment iation = 1) Make a $2,000 sale on account. Must add 6% sales tax2120=2) Record $1,500 cost of goods sold for sale Receive $1,060 cash in advance from customer for
St. Thomas - ACCT - 205
Financial Analysis Project: Best Buy, Inc.Part 2 2) Statement of Cash Flows Operating Activities Total cash from operating activities Investing Activities Capital expenditures New business acquisitions Proceeds from sale of business Total cash for i
St. Thomas - ACCT - 205
Part 5 Investment Potential Obtained the following information from Yahoo Finance (finance.yahoo.com)Last Trade:Splits:17-Jun-85 [3:1], 15-Sep-86 [2:1], 13-Sep-96 [2:1], 19-Sep-00 [2:1] Day's Range: 47.54 - 48.64 48.394:01PM ET 0.94 (1.98%) 47.4
St. Thomas - ACCT - 205
I. % of Sales Method A company has credit sales of $1,000,000 and estimates that 1% of credit sales are bad debts.a. Make the entry to record bad debts expense Accounts re-Allow. for bad Inventory = Common ceivable debt Accounts stock Cash payable
St. Thomas - ECON - 252
by Judd Andrews Q (quantity) 0 1 2 3 4 5 6 7 8 9 10 FC (fixed cost) $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 AFC (avg fixed cost -$530.00 $265.00 $176.67 $132.50 $106.00 $88.33 $75.71 $66.25 $58.89 $53.0
St. Thomas - ECON - 252
by Judd Andrews Q (quantity) 0 1 2 3 4 5 6 7 8 9 10 FC (fixed cost) $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 AFC (avg fixed cost -$530.00 $265.00 $176.67 $132.50 $106.00 $88.33 $75.71 $66.25 $58.89 $53.0
St. Thomas - ECON - 252
by Judd Andrews Q (quantity) 0 1 2 3 4 5 6 7 8 9 10 FC (fixed cost) $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 $530.00 AFC (avg fixed cost -$530.00 $265.00 $176.67 $132.50 $106.00 $88.33 $75.71 $66.25 $58.89 $53.0
UCSB - CHEM - 1BL
Time 0 30.77 51.04 71.49 91.83 112.29 132.6 152.97 173.41 193.68 214.12 234.47 254.76 275.24 295.56Absorbance 0.271 0.208 0.177 0.15 0.127 0.107 0.091 0.077 0.066 0.056 0.049 0.042 0.037 0.033 0.03Time 0 30.77 51.04 71.49 91.83 112.29 132.6 152.9
Pittsburgh - ENGFLM - 0400
Lauren Allen Intro. To Film Moulin RougeTruth, Beauty, Freedom, and most of all, LoveFilm genre criticism, emerging in the late 1960s and early 1970s is in general thought of as rejection of auteuristic theory and criticism. The taxonomy of films
Kutztown - BUS - 120
Company DeWolfe Companies North Coast Energy Hansen Natural Corp. MarineMax, Inc. Nanometrics Incorporated TeamStaff, Inc. Environmental Tectonics Measurement Specialties SEMCO Energy, Inc. Party City Corporation Embrex, Inc. Tech/Ops Sevcon, Inc. AR
Lehigh - ASIA - 078
Columbia - AHIS - C3001
BUILDINGCluny Fontenay Abbey of St. Denis, France Chartres Cathedral Amien Ospedale degli Innocenti Old Sacristy Church of St. Lorenzo Rotonda degli Angeli church of St. Spirito Palace of Parte Guelfa Pazzi chapel in St. Croce Medici palace church
Pittsburgh - ENGFLM - 0400
Lauren Allen Intro. To Film Some Like it HotThe Battle of the Sexes on the Big ScreenThe depiction of femininity produced by and shown to the American culture and its substituent's is unnamed by the subtle way it is usually depicted. Throughout t
Pittsburgh - ENGFLM - 0400
Lauren Allen Intro. To Film Pulp FictionAudience Manipulation On October 14, 1994 Quentin Tarantino's Pulp Fiction was released into theatres in the United States. The movie begins with two lovers sitting at breakfast. Within the few minutes they a
Kutztown - BUS - 120
Hours 4.1 3.1 4.1 10.8 7.2 1.5 4.8 4.1 2.8 6.1 10.4 2.0 8.8 9.5 5.7 5.9 14.8 5.6 12.9 5.9 3.4 5.4 4.3 12.1 4.7 5.7 4.2 3.3 0.7 3.9 1.6 3.9 7.1 4.0 3.7 6.1 4.1 10.3 9.2 3.1 3.0 11.1 6.2 4.4 6.1 3.7 3.5 7.6 5.7 3.1 A.)Column1 Mean 5.706 Standard Erro
Pittsburgh - CHEM - 0330
Sodium Borohydride Reduction: The Conversion of Benzophenone to Diphenylmethanol Chemistry 0330 Hikmat Daghestani Lauren Allen11/12/07Introduction The purpose of this experiment was to reduce benzophenone to diphenylmethanol with the use of sodiu
Pittsburgh - CHEM - 0330
The Extraction of Benzoic Acid from a Mixture Chemistry 0330 Hikmat Daghestani Lauren Allen9/10/07Introduction The purpose of the experiment was to successfully complete the separation of a benzoic acid, methyl orange, and cellulose mixture via e
Pittsburgh - CHEM - 0330
The Recrystallization of Benzoic Acid Chemistry 0330 Hikmat Daghestani Lauren Allen9/17/07Introduction The purpose of the experiment was to explore the concept of purification of the Benzoic Acid that was previously extracted with the use of char
Pittsburgh - CHEM - 0330
Volume of Unknown vs. Temperature76.074.0Temperature in Celsius72.070.068.066.064.0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19Volume (mL)0.5 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 1868.2 71.6 73.2 73.5 73.8 74.0 74.1 74.5
Pittsburgh - CHEM - 0330
Physical Properties: Determining Melting Points and Boiling Points Chemistry 0330 Hikmat Daghestani Lauren Allen9/24/07Introduction The purpose of this experiment was to explore physical properties (the melting points and boiling points) of organ
Pittsburgh - CHEM - 0330
Column and Thin-Layer Chromatography of Spinach Extracts Chemistry 0330 Hikmat Daghestani Lauren Allen10/8/07Introduction The purpose of this experiment was to explore column chromatography using a solid adsorbent as the stationary phase and a li
RIT - MATH - 1
D1D2D31.1188 1.0446 1.2643 1.1288 1.0446 1.2622 1.1288 1.0445 1.2652 1.1189 1.0445 1.2623 1.1189 1.0446 1.2624 Average Average Average 1.12284 1.04456 1.26328 St. Dev St. Dev St. Dev 0.00544086 5.47723E-05 0.00138094 Micrometer D1 D2 D3 0.4543
Pittsburgh - CHEM - 0330
Determination of Unknowns in a Binary Mixture via Fractional Distillation90.080.070.060.0Temp (C)50.040.030.020.010.00.0 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 41 43 45 47 49 51 53 55 57 59 61Volume (mL)Volume
Pittsburgh - CHEM - 0330
The Fractional Distillation of a Binary Mixture Chemistry 0330 Hikmat Daghestani Lauren Allen10/15/07Introduction The purpose of this experiment was to explore fractional distillation and use this technique to separate an unknown binary mixture i
UConn - PHRM - 235
Standards (mg/mL) Absorbance Transmittance @ 500 NM Absorbance2 Absorbance corrected transmit corrected 0.002 0 2 1.8 1.91 9 0.004 9 1.91 1.4 1.7 0.008 54 0.27 0.8 0.5 30 0.012 70 0.15 0.6 0.4 0.016 72 0.14 0.4 0.33 Unknown 1 49 0.31 0.54 0.425 Unkno
Pittsburgh - CHEM - 0330
The Synthesis of Alkenes: The Dehydration of Cyclohexanol Chemistry 0330 Hikmat Daghestani Lauren Allen10/29/07Introduction The purpose of this experiment was to investigate the method of dehydration of organic compounds. With the starting materi
Pittsburgh - CHEM - 0330
The Jones Reaction: The Oxidation of Borneol to Camphor Chemistry 0330 Hikmat Daghestani Lauren Allen11/5/07Introduction The purpose of this experiment was to oxidize borneol (producing camphor,) explore purification of a substance via sublimatio
UConn - PHRM - 235
BSA Protein Absorbance @ 595 NM 1.2Protein Absorbance @ 595 NMLinear (Protein Absorbance @ 595 NM)y = 0.867x + 0.07410.8 Absorbance @ 595 NM0.60.40.20 0 0.2 0.4 0.6 0.8 1 1.2 1.4Concentration of Protein (mg/mL)BSA-DYE STANDARDS (m