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BIPN10025

Course: BIPN 100, Winter 2008
School: UCSD
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Chang Dwayne d2chang@ucsd.edu OH: Wed 12:30P @ Leichtag Lobby Professor: Dr. Fortes BIPN 100 Winter 2008 Week 5 Autonomic Nervous System, Hormones, Signal Transduction Pathways Autonomic Nervous System Organ Heart Effect of sympathetic stimulation (fight/flight) Muscle - rate; force of contraction (1) - Dilates (); constricts () Constricts Constricts (); Dilates (2) Constricted Effect of parasympathetic...

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Chang Dwayne d2chang@ucsd.edu OH: Wed 12:30P @ Leichtag Lobby Professor: Dr. Fortes BIPN 100 Winter 2008 Week 5 Autonomic Nervous System, Hormones, Signal Transduction Pathways Autonomic Nervous System Organ Heart Effect of sympathetic stimulation (fight/flight) Muscle - rate; force of contraction (1) - Dilates (); constricts () Constricts Constricts (); Dilates (2) Constricted Effect of parasympathetic stimulation (rest/digest) - rate; force of contraction (mostly via atrium) - Dilates ---Constricts -- - Coronaries Systemic arterioles - Abdominal - Muscle - Skin Lungs - Bronchi - Blood vessels Adrenal Medulla Liver Sweat glands Salivary glands Gut - Lumen - Sphincter Gall bladder & Bile ducts Kidney Bladder Penis Basal metabolism Eye Pupil - Dilates (2) - Constricts (slightly) catecholamine secretion Release glucose sweating () Mucus & enzyme secretion (, 2) - peristalsis and tone - tone Relaxes urine renin secretion (1) Urinary retention (, 2) Ejaculation (fat breakdown in adipose tissue; ) Dilate () -Small glycogen synthesis -Watery secretion - peristalsis & tone - relaxes Constricts -Release urine Erection -Constrict Adrenergic receptors of the Symp. NS involve G-protein coupled. Receptor 1 2 1 2 3 Adipose tissue; excitatory Location; general effect Targets of symp. NS; excitatory GI tract & pancreas; inhibitory Heart muscle & kidney; excitatory Smooth muscles (arterioles & bronchioles); inhibitory Sensitivity NE > E NE > E NE = E E > NE NE > E Effect on 2nd messenger Activates phospholipase C cAMP cAMP cAMP cAMP Dwayne Chang d2chang@ucsd.edu OH: Wed 12:30P @ Leichtag Lobby Professor: Dr. Fortes BIPN 100 Winter 2008 I. Hormones a. Endocrine System i. Compared to neurotransmitters, hormones are (slower/faster) but (longer/shorter) effects. ii. Endocrine Glands 1. Pineal 2. Pituitary 3. Thyroid 4. Parathyroid 5. Adrenal 6. Pancreas 7. Ovary 8. Testes iii. Organs 1. Hypothalamus 2. Liver 3. Thymus 4. Heart 5. Kidney 6. Stomach 7. Duodenum b. Slow acting hormones via nuclear receptors and protein synthesis i. i.e. ___________ , ______________ , and thyroid hormones c. Fast acting hormones via membrane G-protein bound which receptors activate 2nd messengers i. i.e. peptides, and __________________ . II. Signal Transduction pathways a. G-Protein -> cAMP pathway i. Signal "ON" 1. Signal molecule binds to and activates receptor 2. Receptor activates _______________ GDP is exchanged for GTP G protein dissociates from receptor into G subunits - and G Dwayne Chang d2chang@ucsd.edu OH: Wed 12:30P @ Leichtag Lobby Professor: Dr. Fortes BIPN 100 Winter 2008 3. G s activates _________________________ (AC); G I inhibits AC. 4. AC cAMP (amplification step!) 5. cAMP activates ________________________ (PKA) phosphorylation 6. chain effect activation until reach the final target get specific response (e.g. secretion, division) ii. Signal "OFF" 1. Signal molecule dissociates from receptor receptor is "off" 2. GTPase hydrolyzes the GTP on G to get G -GDP complex. 3. G and G - reunite no more activated AC cAMP (broken down by enzyme _______________________ (PDE) 4. Use _________________ (enzyme) to remove phosphate group from proteins b. G-protein -> IP3 and DAG i. Signal "ON" 1. Signal molecules binds to and activates receptor 2. Receptor activates G protein G protein activates _____________________ (PLC) 3. PLC catalyzes the rxn: PIP2 (phosphatidylinositol bisphosphate) IP3 (inositol triphosphate; polar head) + DAG (diacylglycerol; nonpolar tail) 4. DAG (diffusing on inner side of MB) activates ______________________ (PKC); PKC phosphorylates various proteins. 5. IP3 diffuses through cytoplasm and activates Ca++ channels on endoplasmic reticulum (ER); [Ca++]cytosol ; Ca++ binds to calmodulin; Ca++-calmodulin binds troponin (involved in muscle movement) Dwayne Chang d2chang@ucsd.edu OH: Wed 12:30P @ Leichtag Lobby Professor: Dr. Fortes BIPN 100 Winter 2008 ii. Signal "OFF" 1. Same as for G protein/cAMP pathway 2. To reduce [Ca++]cytosol use Ca++ ATP-driven pump c. Guanyl cyclase i. Catalyzes GTP cGMP ii. Signal "ON" 1. [Ca++]intracellular activates Nitric Oxide Synthase (NOS) 2. NOS catalyzes the rxn: Arg + O2 NO 3. NO activates guanyl cyclase 4. Guanyl cyclase cGMP 5. High levels of cGMP smooth muscle relaxation.
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