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Texas San Antonio - BIO - 2322
Introduction to the Drosophila melanogasterDrosophila melanogasterGENUS-SPECIESBoth the Genus and Species MUST always be underlined - Genus begins with a Capital letter, while Species begins with a Lower Case letter - Commonly called the "Fru
Texas San Antonio - BIO - 2322
Diploid MappingLinked Genes:Genes that are part of the SAME chromosome are said to be LINKED.Genes that are linked will not independently assort and segregate, since these Mendelian laws apply only to genes located on different chromosomes (unlin
Texas San Antonio - BIO - 2322
(Part II of Isolation of DNA from Drosophila)Population GeneticsPopulation GeneticsWhen we study population genetics, we are looking at the frequency of alleles in a given populationThe frequency of these alleles can from 0 (0 %) to 1 (100% ex
Texas San Antonio - BIO - 2322
EXCEPTIONS TO MENDELIAN INHERITANCESimple Mendelian Inheritance 1. One gene is completely dominant to another Exceptions Incomplete or partial dominance an intermediate phenotype results from a cross between parents of contrasting traitsExample: A
Texas San Antonio - BIO - 2322
Genetic Driftgenetic drift- random change in gene frequency due to chance. With a large population chance factors have little effect on gene frequencies. But with small populations, chance factors may produce random changes in gene frequency. Exampl
Texas San Antonio - BIO - 2322
POPULATION GENETICSPopulation: community of sexually interbreeding or potentially interbreeding individuals Gene Pool: consists of all the genes in all their allelic allelic forms in the reproductive gametes of a population. Gene Frequency: the perc
Texas San Antonio - BIO - 2322
Appendix Figure 3. Drosophila melanogaster Dihybrid Crosses Expected Cross #1 and #2 + = Wild Type Eyes w = White Eyes + = Wild Type Body e = Ebony BodyP1: w/w +/+ (White eyes, Wild Type Bodies) X +/+ e/e (Wild Type Eyes, Ebony Bodies) Gametes of P
Texas San Antonio - BIO - 2322
Mutagenesis of Saccharomyces cerevisiaeMaterials required per group: Sterile Microfuge tubes Microfuge tube rack Sterile 200-1000 l pipet tips Sterile 20-200 l pipet tips 20ml of sterile water in a test tube. 8 YPD plates. 6 Sterile plate spreaders.
Texas San Antonio - BIO - 2322
Drosophila melanogaster Katherine Hankins April 24, 2008 Genetics Laboratory Section 06Introduction The purpose of the Drosophila melanogaster, or common fruit fly, experiment is the study of Mendelian genetics and the laws of inheritance. The comp
Texas San Antonio - BIO - 2322
Paola HernandezIntroduction: Drosophila melanogaster is a fruit fly, which is used in biological research, particularly in genetics. The scientific name Drosophila means "lover of dew" which implies that this species requires moist environments to
Texas San Antonio - BIO - 2322
Drosophila Record SheetName Parental genotype Parental genotype Date of F1 Cross F1 genotype F1 genotype phenotype phenotype Date of Cross phenotype phenotypeF2 Cross ResultsPHENOTYPE DATE CountedTOTAL
Texas San Antonio - BIO - 2322
RECOMBINANT DNA SESSION 06: HYBRIDIZATION OF THE BLOTBACKGROUND Melting of DNA sequences The principal forces holding the DNA double helix together are hydrogen bonds between bases in opposite strands and hydrophobic bases stacking interactions betw
Texas San Antonio - BIO - 2322
Elementary Statistics and Probability AnalysisPROBABILITY: the number of times a particular event occurs, divided by the total number of opportunities for the event to occur. Example: If a coin is flipped an infinite amount of time the probability t
Texas San Antonio - BIO - 2322
A)PsPspS PpSs PpSs pS PpSs PpSsB)PSPSPspSpsPPSS PPSs PpSS PpSs PPSs PPss PPSs PpssPs pS psPpSS PpSs ppSS ppSs PpSs PpssppSsppssB)PSPS = 9 Ps = 3 pS = 3ps = 1PS PspSpsPS PS PS PSPS Ps PS Ps PS PS pS pS PS Ps pS
Texas San Antonio - BIO - 2322
POLYGENIC INHERITANCEA trait can be the effect of several independently-assorting gene loci. Each locus may be an active allele which contributes to the phenotype or an inactive allele that does not contribute to the phenotype. Polygenic alleles lac
Texas San Antonio - BIO - 2322
Recombinant DNA Session 05: Nick TranslationBACKGROUND Nick translation is a well-established technique for labeling DNA probes. A probe is a piece of nucleic acid labeled with a tracer ( radioactive or histochemical) that allows an experimenter to
Texas San Antonio - BIO - 2322
Rules for Drosophila Culture1.WASH YOUR HANDS before and after working with fly cultures.2. DECONTAMINATE your work area with Wescodyne or ethanol before and after you work with fly cultures. 3. CLEAN UP ANY SPILLS (medium, yeast, etc.) after yo
Texas San Antonio - BIO - 2322
Recombinant DNA Session 2: Plasmid MiniprepsBACKGROUND The miniprep method, as the name implies, is a rapid, small scale method of obtaining plasmid DNA plus any insert DNA from bacterial cells. Absolute purity is not obtained and usually not essent
Texas San Antonio - BIO - 2322
HUMAN SEX CHROMOSOMES AND BARR BODIESTHE XY SYSTEM In humans and most other mammals, the presence of the Y chromosome appears to determine a tendency for maleness; males are chromosomally XY and females are XX. Since the male produces two types of g
Texas San Antonio - BIO - 2322
Sterile techniqueSterile technique refers to handling live cultures without contaminating either the cultures or the unsuspecting world. It is also vital to obtaining consistent results in genetic experiments. Sources of contamination are everywhere
Texas San Antonio - BIO - 2322
Mendelian Crosses using DrosophilaTest the Following Mendelian Hypothesis for the Drosophila Crosses:1. The 2 or 3 genes in question assortindependently2. The alleles for each gene segregateduring Meiosis3. Sex does not play a role in thein
Texas San Antonio - BIO - 2322
CALCULATING THE SIZE OF PLASMID DNA FRAGMENTS Record the distance each DNA fragment migrated from the well. Sizes should be presented in ascending order, shortest migration (longest fragment) to longest migration (shortest fragment.) P1 (pAMP) KB LAD
Texas San Antonio - BIO - 2322
Texas San Antonio - BIO - 2322
POPULATION GENETICS: A Class Census Of Some Common Human TraitsMost human characteristics are probably determined by many different genes, but some have been associated with a single pair of alleles (one gene). Listed below are some of these traits.
Texas San Antonio - BIO - 2322
Saccharomyces cerevisiaeGeneticists have studied many organisms in an effort to understand the way information is inherited. In general, genetic vectors have been chosen because they are easy to grow, they grow fast, they have lots of traits to sele
Texas San Antonio - BIO - 2322
Texas San Antonio - BIO - 2322
Agarose Gel ElectrophoresisElectrophoresis:It is the separation of a mixture of charged particles in the presence of an electrical field. Examples of Different Types of Electrophoresis: Cellulose Acetate Electrophoresis Polyacrylamide Gel Electr
Texas San Antonio - BIO - 2322
Agarose Gel ElectrophoresisElectrophoresis:It is the separation of a mixture of charged particles in the presence of an electrical field. Examples of Different Types of Electrophoresis: Cellulose Acetate Electrophoresis Polyacrylamide Gel Electr
Texas San Antonio - BIO - 2322
RECOMBINANT DNA SESSION 07: DEVELOPMENT OF THE BLOTBACKGROUND The experimental method used in today's session is functionally equivalent to a Western blot in its detection methodology. Our purpose is to perform a Southern blot to detect a DNA probe
Texas San Antonio - BIO - 2322
BioinformaticsBioinformaticsThe creation and advancement of algorithms, computational and statistical techniques, and theory to solve formal and practical problems arising from the management and analysis of biological data.BioinformaticsData p
Texas San Antonio - BIO - 2322
Isolation of DNA from Drosophila using PCRPCR:Polymerase Chain Reaction (PCR)PCR is a simple, quick method for making many copies of a specific segment of DNA without using cells (in vitro). It is extremely important because of its sensitivity, s
Texas San Antonio - BIO - 2322
Electrophoresis of HbCellulose acetate plates are slowly lowered into the supre-heme buffer and soaked for a few minsTake them out and blot them between the blotting sheets We are going to run 4 bld samples 1. normal 2. sickle cell trait 3. C ha
Texas San Antonio - BIO - 2322
Recombinant DNA Session 1: Transformation of E. coliBACKGROUND Getting foreign DNA to propagate in E. coli is essential to cloning genes. Because these extrachromosomal elements (episomes) are smaller than the bacterial chromosome, they are easy to
Texas San Antonio - BIO - 2322
Restriction DigestionRestriction Enzymes (RE): Bacterial proteins that protect the bacteria's DNA from incoming foreign DNA (e.g. viral DNA). These are endonucleases that cut DNA based on recognition of specific, short DNA sequences called Restri
Texas San Antonio - BIO - 2322
Recombinant DNA Session 04: Southern blottingBACKGROUND Blots! North, South, East (not yet), and West! Blots are an analytical technique to detect the presence and state of nucleic acid and protein sequences. The transfer of DNA from an agarose gel
Texas San Antonio - BIO - 2322
Sordaria Tetrad Scoring SheetAs you examine the asci from either a + x tan or + x gray cross, record the number of parental and non-parental ditypes on the chart below. Use the data to determine the distance between the tan or gray gene and the cent
Texas San Antonio - BIO - 2322
The Lab ReportWhat I expect INTRODUCTION MATERIALS & METHOD RESULTS DISCUSSION APPENDIX REFERENCESINTRODUCTIONTell me about Drosophila melanogaster. Remember to footnote any references. State your hypothesis Briefly, tell me about the e
Texas San Antonio - BIO - 2322
Southern BlotSouthern Blot:It is an analytical technique used to detect the presence and state of DNA sequences. There are 2 other types of Blots:1. Western Blot: Used for protein sequences2. Northern Blot: Used for RNA sequencesSouthern bl
Tulane - HISM - 321
Modern Middles East Notes Islam- One of the 3 great Abrahamic religions means submission Originates in the 7th century Arabia (Saudi Arabia, Yemen, Kuwait etc. ) Muhammad is born in Mecca Born around 570, k now for his honesty, is a merchant Was a de
ASU - ENG - 101
What is Love? Brian WeisBy watching TV we can discover what relationships are like. Shows like Friends and How I Met Your Mother can often provide accurate depictions the types of relationships we see in real life. Sometimes these shows appear very
ASU - ENG - 101
The Rock and Roll Revolution Brian WeisIs anybody out there? Just nod if you can hear me. I am writing to rock and rollenthusiasts out there everywhere. In the 1950s rock and roll came onto the scene. It made an impact on the culture of their era
ASU - ENG - 101
Paper Review Paper owner's name Brian Weis_Reviewer's Name: Brian Weis_ Exchange your rough draft with a partner and analyze it using the bulleted questions in your book (for either question 1 or 3). Each question has a different set of bulleted po
ASU - ENG - 101
Brian Weis English 101 Th 4:40-5:55 Identity Rough Draft Walking aimlessly around the house, I felt out of place. I was at my first party since coming to college. Looking around the room, I tried to find a single face that I could recognize. It was o
ASU - ENG - 101
The Rock and Roll Revolution Brian WeisIn the 1950s and ,60s the United States became witness to a revolution like no other. When Elvis and The Beatles came onto the scene they made an impact on the culture of their era that we still see today. Bef
Tennessee - ENGLISH - 118
Kelly Paley 13 November 2007 Assignment 2 Dr. Sheffield Will History Repeat Itself? The current conflict in the Middle East is a subject in the media that no one can avoid hearing about or seeing in a news story on a daily basis, whether it is about
American - ENGLISH - 101
COMMERICIALIZATION OF CHRISTMAS Celeste AlbertiThe television advertisements always seem to begin a week before Thanksgiving while the sales start the day after. I listen to 92.1, the Christmas Music Cult I like to think of it as, every day at work
American - POLY SCI - 101
CongressWhat do you know about the United States Congress?Congress Versus Parliament What is a major difference between Congress and a parliament?Congress Versus Parliament Congress has the ability to exercise independently of the executive b
Penn State - POLY SCI - 101
Interest GroupsInterest groups do not arise inevitably out of natural causes NAACP 1910 Men and women worked in factories for decades before industrial unionsFour factors explain the rise of interest groups1.Broad economic developments that
American - POLY SCI - 101
CongressThe Organization of Congress: Parties and CaucusesCongress Not a single organization Complex collection of organization While party doesn't matter nearly as much as in a parliament, it is still the number one factor in understanding a
JMU - GHUM - 200
Cloning PowerPoint notes:July 5th, 1996: Dolly, a female sheep, was the first mammal to be cloned from an adult somatic cell. Of the 277 adult udder cells that they used to perform nuclear transfers, twenty-nine grew into developing embryos, which W
JMU - GHUM - 200
P.K. Reilly GHUM 102The History and Major Breakthroughs in CloningA Brief History of Cloning (1880-present) 1880 August Weissmann states genetic information of a cell diminishes with each cell division. 1902 Walter Sutton proves chromosomes hold
JMU - MATH - 103
Inductive and deductive reasoning method of successive differences to find out the last number in a set personal finance convert bases truth tables negate a conditional statement
JMU - GCOM - 123
Study Guide for GCOM 123 Final Exam Spring 2007 Fundamental Concepts (Chapters 1, 3-8, & 11) If a term appears in BOLD it is NOT found in the Rothwell textbook. Communication Competence 1. What is communication? a. A transactional process of sharing
JMU - MATH - 103
3.3: A conditional statement is a compound statement that uses the connective if.then. Written with an arrow p -> q. p is the antecedent and q is the consequent. For conditional: TT=T TF=F FT=T FF=T. Negation of p -> q is p ^ ~q. Conditional as "or"
Portland State - ECE - 241
Portland State - ECE - 241
Portland State - ECE - 241
Portland State - ECE - 241
Portland State - ECE - 241
Miami University - BUS - 101
Chapter Two Concern for Ethical and Societal Issues An organization that wants to prosper over the long term cannot do so without considering business ethics, the standards of conduct and moral values governing actions and decisions in the work envir
Miami University - AER - 118
Chapter 1- Introduction to the Atmosphere Meteorology, Weather, and Climate Meteorology is the scientific study of the atmosphere and the phenomena that we usually refer to as weather. Meteorology is considered one of the earth sciences. Acted on by