2008
Chem365
*
Biochemistry, Huxford-Spring Molecular Cell BiologyI & problem 11 Practice set 1) FromVVP, Chapter Problems 11 1,2,4,5, and18. Chapter Problem and 12 10 19andthe exercises follow: that
For questions 2-5, considerthe enzymemechanism represented the following by chemicalequation:
E + s + E S+
k-1
A) dlESl/dt = 0
k2
E + P
2) Which is NOT an accuratedescriptionof the steadystateapproximation?
BJ krlEllsl= k-'[ES] ftrtEsl +
@ oplrot
D) the rate of ES complexformation and breakdown are equivalent
3 ) Which statement regardingK" is NOT true?
A) It is defined (fr_, fu)lk, as +
B) It is equalto [E][S]/ES] (Kr), if k.1>> lq Q) lt is the substrateconcentrationat 0.5 V."* time (Molar sec-t) @ tt hasunits of concentration/unit
4) Which of the following is NOT equivalentto V*"* during steadystateconditions?
p) KMtz c) ft,lE]r D) dlPl/dt, >> Kna [S]
A
infinity % as [Sl approaches
-
lx(o?s'f
?rtorfr ls-l
1.4'10-trn
required A for: ) V o =0 .1V ," * {.0 23r ,,r |u\ B) Vo = 0.5V,n* 0.1| rrrrM C) Vo = 0.8V."* 0. t{ r".M D) Vo = 0.99V,"* 20.6 ^ 14 0.99V.u^than0.1V.o E) How manytimesmoreATP is needed reach to
Vo: V'"*[s]
3 A)@Vo 0,(V*'* o^l k^-- 0.zt^h r
2o,8r,rla. =
0,D23n^rt4
ffiT
[s] 0.1Vnn --.Vn** + 0,t1+ fsf
gql
0 . o z l ' f+ 0 , t [ s ] = [ s ] n
[s]=ry
0.q
0.02J^14
Huxford-Spring2008 reaction,the initial velocity was determinedat two 7) For an enzyme-catalyzed different concentrationsof the substrate:
(mM) l"Sl 1.0 4.0
Determine the values of K" and V,*.
V"(mM/min)
2.0 2.8
= Vo V** fs)
K^--|sf
g L S J ,: l , 0 r n M
2.0 ula/h; =
A - -
'zKM+ = 2,r^[T'/*^VA 0^;tL.o,*rnJ
&^
MU) + t,o
@ fsl, = (.0 H/t^
2,9 utAlr,^^
7vffit
,'-+,,roD (
)
ku+ (,omhJ
2 g =(zr.-*tI+)
Ku+( f. zKr: 3.2
(
+ { . t K r ^ I l . L =( k n + 3
K", O,tz^nf_ f
= V^* lKr^t2=(t-f,l,L2)+ 2 : V^ry, I
Zup+,*/ ^/ ^e I i--n