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4 Pages
Lab_Appendix_5
Course: BIO 310, Fall 2009School: Bard College
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4: Appendix Inventory of 70 C Freezer Box 042 039 036 047 043 052 001 002 003 055 038 004 048 049 023 Description 15955, 33970 19, 20, 38, 51, 52, 54, 1324, 1325, 5231, 5232, TNT7 50, 53, 92D, 589, 591, 1210, a1Ba, DBY 1324, DBY 1325, DBY 5231, DBY 5232 53, clones, JM103, PK01 53, GLT 53, PK01 A348, PKL1, recombinant plant pathogens Bacillus cereus (Carolina 15-4870) Bacillus subtilis (Carolina 15-4921)...
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4: Appendix Inventory of 70 C Freezer
Box 042 039 036 047 043 052 001 002 003 055 038 004 048 049 023
Description 15955, 33970 19, 20, 38, 51, 52, 54, 1324, 1325, 5231, 5232, TNT7 50, 53, 92D, 589, 591, 1210, a1Ba, DBY 1324, DBY 1325, DBY 5231, DBY 5232 53, clones, JM103, PK01 53, GLT 53, PK01 A348, PKL1, recombinant plant pathogens Bacillus cereus (Carolina 15-4870) Bacillus subtilis (Carolina 15-4921) Branhamella catarrhalis (Carolina 15-4928) Candida albicans, Candida tropicalis EK 11041, PER1, Clone 14, JEK Enterobacter aerogenes (Carolina 15-5030) Escherichia coli B (ATCC 11303) Escherichia coli B (ATCC 11303) Escherichia coli Chi 99 (ATCC 25252) Derived from E. coli K-12 W945. F; requires threonine, leucine, and vitamin B1 for growth; unable to ferment lactose (y point mutation), galactose, xylose, mannitol, and arabinose; resistant to streptomycin. Escherichia coli DH5, Escherichia coli DH5 (pUC18) Escherichia coli DH5a, competent (Rebecca Smith) Escherichia coli EMG 1 or RC 502 or 58-161F (ATCC 23559) Genotype: K-12 met str-r F Escherichia coli EMG 2; K [lambda] Genotype: K-12 his. Strains A, B, and C constructed by BIO 404, Spring 1993. Escherichia coli EMG 2; K [lambda] (ATCC 23716) Genotype: K-12 Wild Type
Date Frozen
02-99 02-99 12-88 03-91 02-99 01-01 01-01 04-89
064 057 051 056
03-93
020
02-89 01-01
Monday, February 12, 2007
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BIOLOGY 310: PROKARYOTIC AND VIRAL GENETICS -- LABORATORY MANUAL APPENDIX 5: INVENTORY OF -70C FREEZER Box 015 016 017 018 032 033 034 027 031 028 026 029 030 021 005 041 035 024 Description Escherichia coli EMG 3; KF51 (CGSC 4477) Genotype: K-12 trpA Escherichia coli EMG 4; KF52 (CGSC 4478) Genotype: K-12 trpB Escherichia coli EMG 5; KF53 (CGSC 4479) Genotype: K-12 trpC Escherichia coli EMG 6; KF54 (CGSC 4480) Genotype: K-12 trpD Escherichia coli EMG 7; 2000 (ATCC 23721) Genotype: K-12 thi pro Escherichia coli EMG 8; 20S0 (ATCC 23722) Genotype: K-12 lac(i+zy+) thi Escherichia coli EMG 9; 3300 (ATCC 23723) Genotype: K-12 lac(iz+y+) thi Escherichia coli EMG 10; C600 (ATCC 23724) Genotype: thi-1 thr-1 leuB6 lacY1 tonA21 supE44 Escherichia coli EMG 10; C600 (ATCC 23724) Genotype: thi-1 thr-1 leuB6 lacY1 tonA21 supE44 Escherichia coli EMG 14; W1655F (ATCC 23737) Genotype: K-12 met -r F Escherichia coli EMG 22; W1655F+ (ATCC 23590) Genotype: K-12 met -r F+ Escherichia coli EMG 27; HfrBr1 (ATCC 23743) Genotype: K-12 Hfr (Broda 1) met Escherichia coli EMG 28 (ATCC 23744) Genotype: K-12 pro thr leu thi str-r F Escherichia coli EMG 29;J62 (ATCC str-rF 23846) Genotype: K-12 pro trp his lac str-r F Escherichia coli HT99 Escherichia coli J53R Escherichia coli Lac (ATCC 15939) Mutates rapidly; reverts to wild. Escherichia coli MV10 Genotype: thi-1 thr-1 leuB6 lacY1 tonA21 supE44 trpE5 . Gift of the Figurski lab, Columbia University. Escherichia coli MV10 pBR322 Genotype: thi-1 thr-1 leuB6 lacY1 tonA21 supE44 trpE5 Plasmid phenotype: Apr, Tcr. Gift of the Figurski lab, Columbia University. Escherichia coli Serotype O78:K80:H12 (ATCC 43896) Escherichia coli VT1259 (MV10 nalr): Genotype: thi-1 thr-1 leuB6 lacY1 tonA21 supE44 trpE5 nalr. Gift of the Figurski lab, Columbia University. Grown for storage in 20 mg/L nalidixic acid. Page A16 Date Frozen 02-89 02-89 02-89 02-89 05-89 05-89 05-89 05-89 05-89 05-89 05-89 05-89 05-89 02-89
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10-93 04-93
Monday, February 12, 2007
1:20:37 PM
BIOLOGY 310: PROKARYOTIC AND VIRAL GENETICS -- LABORATORY MANUAL APPENDIX 5: INVENTORY OF -70C FREEZER Box 058 Description Escherichia coli VT1306 (JA221 RK2 rifr). Genotype: F hsdR lacY leuB6 trpE5 recA1 rifr. Plasmid phenotype: Pnr Kmr Tcr. Gift of the Figurski lab, Columbia University. Grown for storage in 100 mg/L rifampicin. Escherichia coli W1485 (ATCC 12435) Genotype: K-12 Klebsiella pneumoniae (Carolina) Micrococcus luteus (Carolina 15-5155) Mycobacterium smegmatis (Carolina 15-5180) PL PL PL PL PL PPY B1, JM103 Proteus vulgaris (Carolina 15-5240A) Pseudomonas aeruginosa (Carolina 15-5250A) Pseudomonas fluorescens (Carolina 15-5255A), Vibrio fisheri (Carolina) Salmonella typhimurium (ATCC 29629) for Ames test Genotype: HisG46 uvrB rfa Salmonella typhimurium (ATCC 29630) for Ames test Genotype: HisC3076 uvrB rfa Salmonella typhimurium (ATCC 29631) for Ames test Genotype: HisD3052 uvrB rfa Serratia marcescens (Carolina 15-5450A) Serratia marcescens D1 (Carolina 15-5452) Staphylococcus aureus (Carolina 15-555A) Streptococcus faecalis (Carolina 15-5600A) Streptococcus lactis, a.k.a. Lactococcus lactis (Carolina 15-5610) Streptococcus salivarius, a.k.a. Enterococcus salivarius (Carolina) Date Frozen 04-93
022 040 006 007 045 046 050 053 054 044 008 009 010 061 062 063 011 019 012 013 014 037
04-89 02-99 02-99 12-88
02-99 02-99 10-99 01-95 01-95 01-95 03-89 02-89 12-88 02-99 02-99 02-99
Monday, February 12, 2007
Page A17
1:20:37 PM
BIOLOGY 310: PROKARYOTIC AND VIRAL GENETICS -- LABORATORY MANUAL APPENDIX 5: INVENTORY OF -70C FREEZER Box Description Date Frozen
Monday, February 12, 2007
Page A18
1:20:37 PM
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