Ornamental IR-4 Horticulture Program Research Report Cover Sheet
Researcher(s) Yan Chen Affiliation: Trial: 2007 LA LSU AgCenter Hammond Research Station 21549 Old Covington Hwy. Hammond LA 70403 PhoneNumber: [phone] [e-mail] Email: ProjectTitle: Protocol #: PR#
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QRD 400 Crop Safety 07-020 Crop/Plant
Angelonia Angelonia angustifolia Boston Daisy Argyranthemum sp. Begonia Begonia sp. Calibrachoa Calibrachoa sp. Chrysanthemum Dendranthema sp. Pinks Dianthus sp. New Guinea Impatiens Impatiens New Guinea h Geranium Pelargonium sp. Petunia Petunia sp. Periwinkle Vinca sp.
Research Target
Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity Phytotoxicity
Product
QRD 400 Not specified QRD 400 Not specified QRD 400 Not specified QRD 400 Not specified QRD 400 Not specified QRD 400 Not specified QRD 400 Not specified QRD 400 Not specified QRD 400 Not specified QRD 400 Not specified
EPA Reg. # Production Site Status
AgraQuest AgraQuest AgraQuest AgraQuest AgraQuest AgraQuest AgraQuest AgraQuest AgraQuest AgraQuest Greenhouse C Greenhouse Greenhouse C C Greenhouse C Greenhouse C Greenhouse C Greenhouse Greenhouse C C Greenhouse C Greenhouse C
Results of ORD400 phytotoxicity study in spring bedding plants (2007) Brief description of the study set up: Experimental Design: completely randomized design Plot Size: each experimental unit is a 6-pack with 6 plants Replicates: 4 Two applications made 7 days apart with the first application 4 weeks after potting. First application was applied on March 28, second application was made on April 5. Plants had flower buds and flowers (except vinca) prior to first application. Vinca had flowers pior to second application. Applications were made over the top of the plants using application equipment consistent with conventional commercial equipment. Treatments were applied to runoff. Plants were not irrigated until the next day (24 hr) with overhead micro-sprinkler installed in the middle of the benches. Spring trial varieties are petunia, vinca, begonia, dianthus, and impatiens Evaluations: Record plant height & width at initial and final evaluations only. At 1 and 7 days after each application, these are March 29 and April 4 after the first application, and April 5 and April 12 after the second application. Record phytotoxicity on a scale of 0 to 10 (0 = No phytotoxicity; 10 = Complete kill). If appropriate, also include ratings for chlorosis, defoliation, stunting or other growth effects on a scale of 0 to 10 (0 = No effect; 10 = Complete plant affected). If any phytotoxicity is observed in treated plants, take pictures comparing treated and untreated plant material. Recordkeeping: Keep detailed records of weather conditions including temperature and precipitation, soil-type or soil-less media, application equipment, irrigation, liner size, plant height & width, and plant growth stage at application and data collection dates. Treatments: 1X, 2X, and 3.5X rates of QRD 400 0.25 EC and untreated control.
# 1 2 3 4 5 Treatment UTC QRD 400 QRD 400 QRD 400 Standard Rate 0.75 oz/gal 1.3 oz/gal 2.6 oz/gal Conserve Interval (days) 7 day 7 day 7 day 7 day Code 1/ ABC ABC ABC ABC
1/
Look at least 6 bedding, other variety of ornamental plants 1.3 oz/gal = app. 1% vol/vol
Reports:
We did not find significant injury of QRD400 on the foliage of the bedding 5 plants at any rates compared to control (water). We did find damages on flowers of impatiens (moderate), petunia (moderate) and begonia (minor). These injuries are whitish burns of upper petal tissues especially in places where chemical were held before evaporated. Flower buds were not injured and flowers opened after application had normal petal appearances. Table 1: Statistics* and p-values of ANOVA. Variety Foliage Flower Day 2 F test T test F test T test Begonia 0.0528 0.6752 Dianthus 0.1409 0.0950 Impatiens NA <0.0001 <0.0001 (all ratings=0) Petunia 0.6561 0.0004 <0.0001 Vinca NA 0.3339 (all ratings=0) Day 7 F test T test F test T test Begonia 0.4461 0.6982 Dianthus 0.4616 0.2800 Impatiens 0.6422 0.0489 0.0223 Petunia 0.4761 0.0521 Vinca 0.1516 0.3685 Day 9 F test T test F test T test Begonia NA 0.7604 (all ratings=0) Dianthus NA 0.5042 (all ratings=0) Impatiens NA <0.0001 <0.0001 (all ratings=0) Petunia NA 0.0204 0.0136 (all ratings=0) Vinca NA 0.0922 (all ratings=0) Day 12 F test T test F test T test Begonia 0.2522 0.0204 0.0060 Dianthus 0.1966 0.0891 Impatiens 0.7021 0.0231 0.0499 Petunia 0.4761 0.2345 Vinca NA NA (all ratings=0) (all ratings=0) *We used Fisher's Protected LSD to determine if treatments are significantly different compared to untreated control. A treatment is considered significant only when the p-value of the ANOVA F-test and t-teat (for treatment comparison) are both significant at 0.05 levels.
Table 2. Flower injury on begonia flowers were minor injury found 5 days after the second application (day 12) when plants were treated with 3.5X QRD400 (2 vs. 1 in control). Treatment# Treatments 4 2a 2.6 oz/gal 3 1.25 b 1.3 oz/gal 2 1.25 b 0.75 oz/gal 5 1b Conserve 1 1b Control (water) Table 3. Impatiens was the most sensitive species in the trial. Compared to control and Conserve which also injured flower petals, injuries with QRD400 were considered moderate with 3.5X and minor with 2X at 2 days after the first (d3) and the second (d9) application. Plants recovered to some degree on day 7 (d7) and 5 (d12) after the first and second application because flower buds were not injured and flowers ope...