D
How Appendix to Record an Emission Spectrum with the MeasureNet Workstation Recording an Emission Spectrum 1. While at the MeasureNet spectrophotometer press Station Number and type in your Station Number, and press Enter. The screen will display Ready to Scan. 2. Cover the end of the MeasureNet fiber optic cable with a finger and press Zero. Continue to hold your finger over the end of the fiber optic cable until the system indicates Ready to scan. This calibrates the Spectrophotometer to zero intensity (no light). 3. Press the Intensity button. Heat a nichrome wire in a Bunsen burner flame until the wire turns orange. Adjust the position of the burner relative to the fiber optic cable until the intensity reading is somewhere between 2500-4000. Press the Intensity button again. The screen will display Ready to Scan. The Bunsen burner flame must never be closer than 6 inches from the tip or the heat will cause damage to the fiber optics. 4. Pour distilled water on a watch glass. Heat the nichrome wire until it glows bright orange. 5. Place the watch glass near the air intake of the Bunsen burner, tilting it so the water moves to the edge of the watch glass. Immerse the hot wire into the water on the watch glass and the water will spatter into the burner intake. 6. The process should be repeated 3 to 4 times. 7. Pour approximately 1ml of a standard metal ion or an unknown metal ion solution on a watch glass. 8. Heat the nichrome wire until it glows orange.
Appendix D
How to Record an Emission Spectrum with the MeasureNet Workstation 9. Positioning the watch glass with the sample near the burner air intake, immerse the hot wire into the sample to spatter it into the flame. lab Your partner must press the Sample button on the spectrophotometer to record the emission spectrum as soon as the hot nichrome wire contacts the sample. 10. Pour the rest of the metal ion solution in the Waste Container and rinse the watch glass thoroughly with distilled water. Record the color of the flame in the table on the Data Sheets. 11. Go back to your MeasureNet workstation. Examine the emission spectrum on your workstations display. If the intensity exceeds the parameters you entered, press the Setup button, then press the F2 button, enter new minimum and maximum values, the press the Display button to accept your changes. MeasureNet will re-plot the emission spectrum. 12. Press the File Options button. Press the F3 button to save the scan as a tab delimited file. You will be asked to enter a 3 digit code. Then press the Enter button. The name of your file will be saved as your station number plus the 3 digit number you entered. Record this file name. 13. Save the file to a floppy disk, a flash drive, or to the lab web server. 14. Press the Display button on the workstation to clear the previous scan, and to ready the Workstation to record another spectrum. 15. Go back to the MeasureNet spectrophotometer. Repeat Steps 1-15 for each of the remaining standard solutions, or for an unknown water sample. Be sure to rinse the burner by spattering water through it 3-4 times after the emission spectrum of each standard solution or unknown water sample spectrum is recorded.
Appendix D
How to Record an Emission Spectrum with the MeasureNet Workstation 16. Turn off the MeasureNet workstation and return to the procedure in the corresponding laboratory experiment.