Documents Found!
As seen in
Less Work, Better Grades
Join
Course Hero
Access
best resources
Ace
your classes
Ace your courses with Course Hero!

Submit your homework question or assignment here:
352 Tutors are online
 
We are so confident that you will love our service, we will answer your first homework question for FREE!
*  Attach Assignment (optional):
 
Our current time in (CST) is
 
Study Smarter, Score Higher
View This Document For FREE!
 
Document Content (unformatted)
Course Hero has millions of student submitted documents similar to the one below including study guides, homework solutions, papers, exam answer keys and textbook solutions.
diffusion The blot protocol Use diffusion blotting - the way we used to do this was to use the cooling plate in the Multiphor apparatus heated to about 50 C and then place a backed gel (usually plastic backed) on to the cooling plate (using water or a light parafin oil underneath the plastic back to provide good thermal contact). The blotting membrane is then placed on the gel surface (the membrane having been activated and soaked in Towbin buffer) and then wetted filter paper ( 5 thick sheets) placed on top of the membrane (wetted also in Towbin buffer) and finally topped off with dry filter paper (x 5 again) (all the same size as the gel) and covered in parafilm with some kind of weight on top - we did a time course using rainbow markers to assess transfer effciency against time and think 50 minutes was optimal for our set - up you should check this yourself. The nice thing with this set up tis that diffusion blotting (by heating you will speed up the process and is called a thermal blot I think) is a lot less efficient than electroblotting (only about 30%) but this is great as your antibody does not need a lot of protein/ target to be able to detect it and then there is plenty of protein left behind in your gel so that you can post stain with silver and then the blot will exactly match the spot pattern as you have stabilised the gel size by using the plastic backing We used to love this method - maybe I should get an application note done ? The plastic backing I talk of is very special and is called GelBond - we sell this but there is only one manufacturer of this product in the world called FMC and they charge a premium for the stuff
Find millions of documents here - Study Guides, Homework Solutions, Papers, Exam Answer Keys and more. Course Hero has millions of course related materials that will enable you to learn better, faster and get an A in all your courses.
Below is a small sample set of documents:

BU >> GENOMICS >> 10 (Fall, 2009)
Bioinformatics at Boston University Four years ago, Boston University established one of the first Ph.D. programs in Bioinformatics in the nation. Our doctoral training program in Bioinformatics is supported by an IGERT grant from the National Scienc...
BU >> GENOMICS >> 10 (Fall, 2009)
PROTEINS: Structure, Function, and Genetics 51:172188 (2003) Pharmacophore-Based Molecular Docking to Account for Ligand Flexibility Diane Joseph-McCarthy,* Bert E. Thomas IV, Michael Belmarsh, Demetri Moustakas, and Juan C. Alvarez Wyeth Research, ...
BU >> GENOMICS >> 10 (Fall, 2009)
PROTEINS: Structure, Function, and Bioinformatics 59:205220 (2005) A Pharmacophore-Based Evolutionary Approach for Screening Selective Estrogen Receptor Modulators Jinn-Moon Yang* and Tsai-Wei Shen Department of Biological Science and Technology, an...
BU >> GENOMICS >> 10 (Fall, 2009)
Journal of Computer-Aided Molecular Design, 16: 883902, 2002. KLUWER/ESCOM 2003 Kluwer Academic Publishers. Printed in the Netherlands. 883 Distilling the essential features of a protein surface for improving protein-ligand docking, scoring, and v...
BU >> GENOMICS >> 10 (Fall, 2009)
Predicting Molecular Interactions in silico: I. A Guide to Pharmacophore Identication and its Applications to Drug Design Oranit Dror1 Alexandra Shulman-Peleg1 Ruth Nussinov2,3 Haim J. Wolfson1 , , , 1 School of Computer Science, Tel Aviv Univers...
BU >> GENOMICS >> 10 (Fall, 2009)
PROTEINS: Structure, Function, and Genetics 51:189 202 (2003) Automated Generation of MCSS-Derived Pharmacophoric DOCK Site Points for Searching Multiconformation Databases Diane Joseph-McCarthy* and Juan C. Alvarez Wyeth Research, Biological Chemis...
BU >> GENOMICS >> 10 (Fall, 2009)
J. Med. Chem. 1999, 42, 3251-3264 3251 New 4-Point Pharmacophore Method for Molecular Similarity and Diversity Applications: Overview of the Method and Applications, Including a Novel Approach to the Design of Combinatorial Libraries Containing Pri...
BU >> GENOMICS >> 10 (Fall, 2009)
J. Med. Chem. 2005, 48, 121-133 121 Interaction Profiles of Protein Kinase-Inhibitor Complexes and Their Application to Virtual Screening Claudio Chuaqui, Zhan Deng, and Juswinder Singh* Computational Drug Design Group, Department of Research Infor...
BU >> GENOMICS >> 10 (Fall, 2009)
J. Chem. Inf. Comput. Sci. 2003, 43, 2163-2169 2163 Using Ensembles to Classify Compounds for Drug Discovery J. Kevin Lanctot,* Santosh Putta, Christian Lemmen, and Jonathan Greene Deltagen Research Laboratories, Inc., 740 Bay Road, Redwood City, C...
BU >> GENOMICS >> 10 (Fall, 2009)
J. Med. Chem. 2004, 47, 2743-2749 2743 Finding More Needles in the Haystack: A Simple and Efficient Method for Improving High-Throughput Docking Results Anthony E. Klon, Meir Glick, Mathis Thoma, Pierre Acklin, and John W. Davies* Novartis Institut...
BU >> GENOMICS >> 10 (Fall, 2009)
Erratum In the December 2004 (Volume 9, Number 8) article by Prabha S. Karnachi and Frank K. Brown, Practical Approaches to Efficient Screening: Information-Rich Screening Protocol, the legend for Figure 4 was printed incorrectly on page 684. The cor...
BU >> GENOMICS >> 10 (Fall, 2009)
J. Med. Chem. 2004, 47, 337-344 337 Structural Interaction Fingerprint (SIFt): A Novel Method for Analyzing Three-Dimensional Protein-Ligand Binding Interactions Zhan Deng, Claudio Chuaqui, and Juswinder Singh* Department of Structural Informatics,...
BU >> GENOMICS >> 10 (Fall, 2009)
J. Chem. Inf. Comput. Sci. 2003, 43, 47-54 47 Luddite: An Information-Theoretic Library Design Tool Jennifer L. Miller,*, Erin K. Bradley, and Steven L. Teig Deltagen, Inc., 740 Bay Road, Redwood City, California 94063, and Cadence Design Systems, ...
BU >> GENOMICS >> 10 (Fall, 2009)
Journal of Molecular Graphics and Modelling 20 (2002) 469477 Coupling structure-based design with combinatorial chemistry: application of active site derived pharmacophores with informative library design John E. Eksterowicz a, , Erik Evensen a , Ch...
BU >> GENOMICS >> 10 (Fall, 2009)
6804 J. Med. Chem. 2004, 47, 6804-6811 Ph4Dock: Pharmacophore-Based Protein-Ligand Docking Junichi Goto, Ryoichi Kataoka, and Noriaki Hirayama*, Computational Science Department, Ryoka Systems Inc., 1-5-2 Irifune, Urayasu, Chiba 279-0012, Japan, an...
BU >> GENOMICS >> 10 (Fall, 2009)
This article has been modified from the original printed version. Originally the caption for Figure 4 on page 684 had incorrect colors as the key for the figure. They are now correct. Practical Karnachi Approaches ARTICLEand Brown to Efficient Scree...
BU >> GENOMICS >> 10 (Fall, 2009)
10.1007/s00894-004-0201-1 Seite 1 von 29 Journal of Molecular Modeling Computational Chemistry - Life Sciences - Advanced Materials - New Methods Springer-Verlag 2004 10.1007/s00894-004-0201-1 Original Paper ProPose: a docking engine based on a ...
BU >> GENOMICS >> 10 (Fall, 2009)
application note gel, blot, and microarray analysis system Imaging two-dimensional protein gels stained with SYPRO Ruby Typhoon Variable Mode Imager Key words: proteomics, imaging, uorescence, 2-D electrophoresis, gel stains, SYPRO Ruby, Typhoon Tw...
BU >> GENOMICS >> 10 (Fall, 2009)
A M E R S H A M P H A R M A C I A B I O T E C H IPGphor Isoelectric Focusing System User Manual 80-6415-35/Rev. A1/11-00 I P G p h o r I s o e l e c t r i c F o c u s i n g S y s t e m U s e r M a n u a l 1. IPGphor F...
BU >> GENOMICS >> 10 (Fall, 2009)
P h a r m a c i a B i o t e c h Immobiline DryStrip Reswelling Tray User Manual 80637564 Rev B/5-97 English Important user information Please read this entire manual to fully understand the safe and effective use of this product. ...
BU >> GENOMICS >> 10 (Fall, 2009)
Nucleic Acids Research, 2005, Vol. 33, Web Server issue W1W4 GEMS: a web server for biclustering analysis of expression data Chang-Jiun Wu1 and Simon Kasif1,2,* 1 Program in Bioinformatics and 2Department of Biomedical Engineering, Boston Universi...
BU >> GENOMICS >> 10 (Fall, 2009)
RankGene: Identication of Diagnostic Genes Based on Expression Data Yang Su T. M. Murali Vladimir Pavlovic Michael Schaer Simon Kasif Bioinformatics Program, Boston University, Boston MA 02215, Department of Computer Science, Rutgers University, Pi...
BU >> GENOMICS >> 10 (Fall, 2009)
APBiotech, RW, 05.08.99 Test Sample for 2-D Electrophoresis There is a need for a test sample for 2-D Electrophoresis because of the following reasons: Courses Demonstration of the Amersham Pharmacia Biotech 2-D electrophoresis equipment and consu...
BU >> GENOMICS >> 10 (Fall, 2009)
Immobiline DryStrip Visualization of pH gradients 18-1140-60 Immobiline DryStrip as the first di Setting the standard Products from Amersham Pharmacia Biotech set the standard for successful, highly reproducible 2-D electrophoresis, using Immobilin...
BU >> GENOMICS >> 10 (Fall, 2009)
lab_front.qxd 6/25/01 1:47 PM Page 1 Agilent Direct-Label cDNA Synthesis Kit Protocol Users Guide For use with cat. nos. G2555A and G2557A Version 1.0 June 2001 lab_front.qxd 6/25/01 1:47 PM Page 2 Notices 2001 Agilent Technologies, Inc. ...
BU >> GENOMICS >> 10 (Fall, 2009)
Background Analysis and Cross Hybridization Application Pius Brzoska, Ph.D. Abstract Microarray technology provides a powerful tool with which to study the coordinate expression of thousands of genes in a single hybridization reaction. However, prob...
BU >> GENOMICS >> 10 (Fall, 2009)
Available Soon! Agilent\'s Human 1 cDNA Microarray Kit As part of Agilent\'s Gene Expression Analysis Solution, Agilent Technologies introduces the first in a series of off-the-shelf, ready-tohybridize microarray kits. With amplified cDNA from Incyte\'s...
BU >> GENOMICS >> 10 (Fall, 2009)
Electroendosmosis (EEO) Active water transport towards the ends of the IPG strip occurs during IEF. This is exacerbated by the presence of charged molecules in the sample, or breakdown products found in poor grades of urea, all of which accumulate at...
BU >> GENOMICS >> 10 (Fall, 2009)
The easy way to treat glass plates with bind silane so that you can bind your gel to them BEFORE running is here Preparing the gel 3 3.1 Introduction Spot picking with the Ettan Spot Picker requires that gels are precast on backing (e.g. Ettan DALT I...
BU >> GENOMICS >> 10 (Fall, 2009)
APBiotech, RW,6 April, 1999 APPENDIX: Sleeping Beauty and Prince Buffer 1) Fix in 200 mM Imidazole containing 0.1 % SDS: - 2.72 g Imidazole + 0.2 g SDS, dissolve in 200 mL dist. water. 15 min with continuous shaking. 2) Rinse with dist water. 3) St...
BU >> GENOMICS >> 10 (Fall, 2009)
Acid Violet Staining of Immobiline Gels and Strips Solutions *20 % TCA (dilute 40ml of 100% TCA with 160 ml dd water) *3% phosphoric acid (21 ml 85% phosphoric acid in 1L water) *11% phosphoric acid (76 ml of 85% phosphoric| acid in 1L water) *1% Aci...
BU >> GENOMICS >> 10 (Fall, 2009)
The Tris tricine buffer system for the DALT 12 Yesterday i have run the first experiment with ETTAN DALT II gels using tricine buffer in the upper tank for improved resolution of small peptides. It works well! The result is not yet perfect to show, b...
BU >> GENOMICS >> 10 (Fall, 2009)
Network-Based Analysis of Affected Biological Processes in Type 2 Diabetes Models Manway Liu1[*, Arthur Liberzon1[, Sek Won Kong2,3[, Weil R. Lai4, Peter J. Park3,4, Isaac S. Kohane3,5, Simon Kasif1,3,6* 1 Department of Biomedical Engineering, Boston...
BU >> GENOMICS >> 10 (Fall, 2009)
Gene Expression Module Discovery Using Gibbs Sampling Chang-Jiun Wu1* terrence@bu.edu Yutao Fu1* bibin@bu.edu T. M. Murali2 murali@cs.vt.edu 1 Simon Kasif 1,3 kasif@bu.edu Boston University Bioinformatics Program, Boston, MA02215, USA. Department...
BU >> GENOMICS >> 10 (Fall, 2009)
YLR418C YOL145C YLR418C YOR123C YLR418C YBR279W YLR418C YML069W YLR418C YGL244W YLR418C YGL207W YER164W YIL035C YER164W YOR061W YER164W YGL019W YER164W YOR039W YER164W YML069W YER164W YGL207W YOL145C YOR123C YOL145C YBR279W YOL145C YML069W YOL145C YG...
BU >> GENOMICS >> 10 (Fall, 2009)
On the normalization of RNA equilibrium free energy to the length of the sequence Dmitri Pervouchine, Joel Graber and Simon Kasif Abstract There is no single denition of stability for RNA secondary structures. In this paper we present an approach bas...
BU >> GENOMICS >> 10 (Fall, 2009)
user manual Ettan DALT II Gel, 12.5 and Ettan DALT II Buffer Kit Polyacrylamide gels and buffers for the second dimension of 2-dimensional electrophoresis um 71-5019-56 Edition AA For further information: Asia Pacific Tel: +852 2811 8693 Fax: +852...
BU >> REV >> 2 (Fall, 2009)
Boston University STC-B Bill of Materials - 8/27/01 Used 1 1 181 1 2 1 5 1 1 6 12 4 1 10 1 1 9 2 1 3 12 1 1 1 1 2 1 1 Part Type 470pF ceramic capacitor 0805 10nF ceramic capacitors 0603 0.1uF ceramic capacitors 0603 1.0uF ceramic capacitor 1206 10uF ...
BU >> MB >> 9 (Fall, 2009)
Sheet1 Item Qty 1 3 186 0.1 Value Tolerance Footprint MIRE 603 Refs P10 P11 P9 C100 C101 C102 C103 C104 C105 C106 C107 C108 C109 C110 C111 C112 C113 C114 C115 C116 C117 C118 C119 C120 C121 C122 C123 C124 C125 C126 C127 C128 C129 C130 C131 C132 C133 C...
BU >> CERNDEC >> 02 (Fall, 2009)
HCAL Data Concentrator Readout Unit Working Group DCC Status Report E. Hazen, J. Rohlf, S.X.Wu Boston University RUWG at CERN Dec 2002 E. Hazen Data Concentrator Card Review of Functionality: Receive 1 block per L1A from up to 15 HTRs Add h...
BU >> AM >> 188 (Fall, 2009)
E 8 6 M O N R E L E A S E N O T E S - Current Bugs Reported: - E86MON Revision history: 3.42 -- Eliminated the \'waitstates\' permanent variable which determines how many UMCS wait states there are and replaced it with \'umcs...
BU >> AM >> 188 (Fall, 2009)
ADVANCE INFORMATION AMD Am188ES MICROCONTROLLER BLOCK DIAGRAM INT2/INTA0* INT3/INTA1/IRQ CLKOUTA INT6INT4* INT1/SELECT INT0 NMI PWD* TMROUT0 TMRIN0 TMROUT1 DRQ0* DRQ1* CLKOUTB TMRIN1 X2 X1 VCC GND Timer Control Unit Interrupt Control Unit Puls...
BU >> RUN >> 62024 (Fall, 2009)
HBHE Sliding Pair Sum Maxes 10 5 HBHE Sliding Pair Sum Maxes Entries Mean RMS 201180 8.546 0.4678 104 10 3 102 10 1 0 2 4 6 8 10 12 14 16 18 20 ...
BU >> RUN >> 62024 (Fall, 2009)
HF Sliding Pair Sum Maxes HF Sliding Pair Sum Maxes Entries Mean RMS 44592 9.589 0.6436 104 103 102 10 1 0 2 4 6 8 10 12 14 16 18 20 ...
BU >> RUN >> 62024 (Fall, 2009)
HO Sliding Pair Sum Maxes HO Sliding Pair Sum Maxes Entries Mean RMS 38291 8.463 0.4248 104 103 102 10 0 2 4 6 8 10 12 14 16 18 20 ...
BU >> MB >> 9 (Fall, 2009)
VME 9U Motherboard E. Hazen - Boston University August 17, 2004 Abstract This document describes a 9Ux400mm VME64x-compatible motherboard designed to be used for various data-acquisition applications. It houses up to 6 PC-MIP and 2 expanded PMC-type...
BU >> PROTO >> 2 (Fall, 2009)
Bio Sensor Project PicoBlaze Firmware Requirements - PRELIMINARY E. Hazen Revised Feb 6, 2009 The PicoBlaze firmware in the Bio Sensor will communicate with a host computer via an RS-232 serial link, and provide for control of the device. Control lo...
BU >> LX >> 500 (Fall, 2009)
CAS LX 500 Topics in Linguistics Fall 2000 Paul Hagstrom Pidgins and creoles (Bickerton 1981) Step one: Innovation in creoles. Language Universals September 13, 2000 Week 2b: Pidgins and creoles, day one HPE (HAWAIIAN PIDGIN ENGLISH) & HCE (HAWAIIA...
BU >> LX >> 502 (Fall, 2009)
CAS LX 502 Semantics 9b. Presupposition, entailments, and implicatures 10.2, 11 Truth and falsity ! Pat is hungry. ! True under some possible circumstances, false under others. Pat is hungry Pat is not hungry ! True if the \"actual world\" is in t...
BU >> LX >> 400 (Fall, 2009)
CAS LX 400 Second Language Acquisition The standard story Pidginverbal system used by linguistically diverse people stuck with the need to communicate. Idea: Pidgins are unsystematic, simple, without distinctions of tense, modality, aspect, genera...
BU >> LX >> 502 (Fall, 2009)
Possible worlds CAS LX 502 4a. Presupposition and assertion 4.5- It is uncontroversially true that things might have been otherwise than they are. I believe, and so do you, that things could have been different in countless ways. But what does thi...
BU >> CS >> 101 (Fall, 2009)
Chapter 1: Computers: A First Look How do computers affect us in our daily lives? The Computer Continuum 1-1 Computers: A First Look s In this chapter: How do computers pervade our every day lives? What is considered a computer and what is n...
BU >> CS >> 101 (Fall, 2009)
Chapter 3: Computer Hardware Components: CPU, Memory, and I/O What is the typical configuration of a computer sold today? The Computer Continuum 1-1 Computer Hardware Components n In this chapter: How did the computer become known as the stored-...
BU >> CS >> 101 (Fall, 2009)
Chapter 1: Computers: A First Look How do computers affect us in our daily lives? The Computer Continuum 1-1 Computers: A First Look n In this chapter: How do computers pervade our every day lives? What is considered a computer and what is ...
BU >> CS >> 101 (Fall, 2009)
Chapter 9: Multimedia Most of the outside information entering our brains, enters through our eyes or our ears. They are, indeed, input devices through which our world communicates with us. The Computer Continuum 9-1 Multimedia n In this chapter...
BU >> CS >> 101 (Fall, 2009)
Chapter 8: The Internet and the Web: World Wide Transformation The Internet grew from a grass-roots society into a global community. Keeping this in mind, whose rules will we need to follow when we make use of the World Wide Web? The Computer Contin...
BU >> CS >> 440 (Fall, 2009)
/* Allocate memory for alpha and beta. delete below. You can use new here if you use */ alpha = (double *)malloc(hmm->T * sizeof(double *); for (i = 0; i < hmm->T; i+) { alpha[i] = (double *)malloc(hmm->N * sizeof(double); } beta = (double *)malloc(...
BU >> CS >> 101 (Fall, 2009)
<?php $score = 0; $message = \"; if ( $answer1 = \"1\" ) { $score+; } else { echo \"<p>You got Question 1 wrong. The correct answer was EXPLAIN ANSWER.</p>\"; } if ( $answer2 = \"1\" ) { $score+; } else { echo \"<p>You got Question 2 wrong. The ...
BU >> CS >> 101 (Fall, 2009)
P3 200 270 255 207 237 245 206 236 244 205 235 243 204 234 242 203 233 241 202 232 240 202 232 240 204 234 242 205 235 243 205 235 243 203 233 241 200 230 238 199 229 237 202 232 240 204 234 242 194 226 237 194 226 237 195 227 238 196 228 239 197...
BU >> XLC >> 8 (Fall, 2009)
IBM XL C/C+ Advanced Edition V8.0 for Blue Gene IBM XL Fortran Advanced Edition V10.1 for Blue Gene Using the XL Compilers for Blue Gene SC10-4310-00 IBM XL C/C+ Advanced Edition V8.0 for Blue Gene IBM XL Fortran Advanced Edition V10.1 for Blue Ge...
BU >> CS >> 511 (Fall, 2009)
Analyzing SnBench Components using Alloy CS511 term paper proposal by Vatche Ishakian [visahak@cs.bu.edu] and Michalis Potamias [mp@cs.bu.edu] Abstract The SnBench is an ongoing project at Boston University. The aim of this project is to provide a p...
BU >> CS >> 111 (Fall, 2009)
Classes as Blueprints: How to Define New Types of Objects Computer Science 111 Boston University Spring 2009 David G. Sullivan, Ph.D. Types of Decomposition When writing a program, it\'s important to decompose it into manageable pieces. We\'ve alre...
BU >> CS >> 111 (Fall, 2009)
CAS CS 111: Introduction to Computer Science I Boston University Spring 2009 Syllabus Description: This first course in computer science develops basic skills in computer programming using the Java programming language. The course is suitable for ma...
BU >> CS >> 105 (Fall, 2009)
CAS CS 105: Introduction to Databases and Data Analysis Boston University Spring 2009 Syllabus Description: This course will examine how collections of data are organized, stored, and analyzed. Topics include: relational databases and the SQL query ...
BU >> CS >> 105 (Fall, 2009)
Programming in Python II: Working with Numbers Computer Science 105 Boston University Spring 2009 David G. Sullivan, Ph.D. Data Types in Python In Python and other programming languages, different kinds of data are stored and manipulated different...
BU >> CS >> 105 (Fall, 2009)
Database Fundamentals Computer Science 105 Boston University Spring 2009 David G. Sullivan, Ph.D. Measuring Data: Bits and Bytes Bit = 0 or 1 One byte is 8 bits. example: 01101100 Other common units: approximate size name kilobyte (KB) 1000 byt...
BU >> CS >> 105 (Fall, 2009)
Data Mining III: Numeric Estimation Computer Science 105 Boston University Spring 2009 David G. Sullivan, Ph.D. Review: Numeric Estimation Numeric estimation is like classification learning. it involves learning a model that works like this: inpu...
BU >> CS >> 105 (Fall, 2009)
The Relational Model Computer Science 105 Boston University Spring 2009 David G. Sullivan, Ph.D. What Is a Data Model? A formal way of describing: pieces of data (data items) relationships between data items constraints on the values of data it...
BU >> CS >> 111 (Fall, 2009)
Tackling an Intractable Problem Computer Science 111 Boston University Spring 2009 David G. Sullivan, Ph.D. Algorithm Analysis Revisited Recall that we can group algorithms into classes (n = problem size): name constant time logarithmic time linea...
BU >> CS >> 105 (Fall, 2009)
Programming in Python V: Accessing a Database Computer Science 105 Boston University Spring 2009 David G. Sullivan, Ph.D. Accessing a Database from Python Import the necessary Python module: import sqlite3 Connect to the database and create a da...
What are you waiting for?
View This Document For FREE!