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lecture notes-biochemistry-3-Lipids

Course: CHE 461, Fall 2009
School: Air Force Academy
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are Lipids Lipids energy storage in cell membrane and regulators of cell metabolism. Lipids are hydrophobic biological compounds that are insoluble in water, but soluble in nonpolar solvent such as benze, chloroform and ether. They are present in the nonaqueous biological phase such as plasma membrane. Cells can alter the mix of lipids in their membrane to compensate for changes in temperature or to increase...

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are Lipids Lipids energy storage in cell membrane and regulators of cell metabolism. Lipids are hydrophobic biological compounds that are insoluble in water, but soluble in nonpolar solvent such as benze, chloroform and ether. They are present in the nonaqueous biological phase such as plasma membrane. Cells can alter the mix of lipids in their membrane to compensate for changes in temperature or to increase their tolerance to the presence of chemical agents such as ethanol. Lipids Fatty Acids : The major component in most lipids made of a straight chain of hydrophobic hydrocarbon group, with a carboxyl group (hydrophilic) at the end. A typical saturated fatty acid has the form of CH3-(CH2)n COOH Where n is typically between 2 and 20, such as acetic acid CH3COOH. A typical unsaturated fatty acid contain double C=C- , or triple bonds on the hydrocarbon chain, such as Oleic acids: CH3-(CH2)7-HC=CH-(CH2)7-COOH Important Lipids - Fats Fats are lipids that are esters of fatty acids with glycerol. glycerol Fatty acids fat Fats Fats play a vital role in maintaining healthy skin and hair, insulating body organs against shock, maintaining body temperature, and promoting healthy cell function. They also serve as energy stores for the body and can serve as biological fuel-storage molecules. Fats are broken down in the body release to glycerol and free fatty acids. glycerol can be converted to glucose by the liver and thus used as a source of energy. The fatty acids are a good source of energy for many tissues, especially heart and skeletal muscle. Lipids - Phospholipids Phospholipids such as glycerophospholipids are built on a glycerol core to which are linked two fatty acid-derived "tails" by ester linkages and one "head" group by a phosphate ester linkage. Phospholipids are key components to control the entry or exit of molecules in the cell membrane. Lipids - Steroids A steroid is a lipid characterized by a carbon skeleton with four fused rings. Different steroids vary in the functional groups attached to these rings. Steroids Their most important role in most living systems is as hormo...

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Air Force Academy - CHE - 461
Nucleic Acids - RNA and DNANucleic acid is a complex, high-molecular-weight biochemical macromolecule composed of nucleotide chains that convey genetic information. The most common nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (
Air Force Academy - CHE - 461
Cell NutrientsNutrients required by cells can be classified in two categories: 10-4 M. C, N, O, H, S, P, Mg 2+, and K+. are needed in concentrations larger than-10-4 M. Mo, Zn, Cu, Mn, Ca, Na, vitamins, growth hormones and metabolic precursors.
Air Force Academy - CHE - 461
Cell NutrientsNutrients required by cells can be classified in two categories: - Macronutrients are needed in concentrations larger than 10-4 M. C, N, O, H, S, P, Mg 2+, and K+. - Micronutrients are needed in concentrations less than 10-4 M. Mo, Zn,
Air Force Academy - CHE - 461
Outline of EnzymesIntroduction - Features of enzyme catalysis Enzyme kinetics - Models for enzyme kinetics - Effect of pH and Temperature Immobilized Enzyme System - Method of immobilization - Diffusional limitations Medical and Industrial Utilizati
Air Force Academy - CHE - 461
Outline of EnzymesIntroduction - Features of enzyme catalysis Enzyme kinetics - Models for enzyme kinetics - Effect of pH and Temperature Immobilized Enzyme System - Method of immobilization - Diffusional limitations Medical and Industrial Utilizati
Air Force Academy - CHE - 461
Enzyme KineticsEnzyme Kinetics: Study the rate of enzyme catalyzed reactions. - Models for enzyme kinetics - Michaelis-Menten kinetics - Inhibition kinetics - Effect of pH and TemperatureEnzyme KineticsMichaelis-Menten kinetics or saturation kine
Air Force Academy - CHE - 461
Substrate Concernation versus Time25 Substrate Concentration (M) 20 15 10 5 005101520253035Time (min)Substrate Concernation versus Time25 Substrate Concentration (M) 20 15 10 5 005101520253035Time (min)Use in
Air Force Academy - CHE - 461
Glucoamylase Activity DeterminationEx. One unit of activity of glucoamylase is defined as the amount of enzyme which produces 1 mol of glucose per min in a 4 % solution of Lintner starch at pH4.5 and at 60oC. To measure the amount of glucoamylase in
Air Force Academy - CHE - 461
Example: During a test kinetics of enzyme-catalyzed reaction, the following data E0 (g/l)T(oC) I (mmol/ml) V (mmol/mi-min) S(mmol/ml) 0.92 30 0 0.1 1.64 0.92 30 0 0.02 0.9 0.92 30 0 0.01 0.58 0.92 30 0.6 0.1 1.33 0.92 30 0.6 0.03 0.8 0.92 30 0.6 0.02
Air Force Academy - CHE - 461
Factors Affecting Enzyme Kinetics pH effects - on enzymes - enzymes have ionic groups on their active sites. - Variation of pH changes the ionic form of the active sites. - pH changes the three-Dimensional structure of enzymes. - on substrate - some
Air Force Academy - CHE - 461
Immobilized Enzyme SystemsEnzyme Immobilization: To restrict enzyme mobility in a fixed space.Immobilized Enzyme SystemsEnzyme Immobilization:- Easy separation from reaction mixture, providing the ability to control reaction times and minimize t
Air Force Academy - CHE - 461
Immobilized Enzyme SystemsEnzyme Immobilization: To restrict enzyme mobility in a fixed space.Immobilized Enzyme SystemsEnzyme Immobilization:- Easy separation from reaction mixture, providing the ability to control reaction times and minimize t
Air Force Academy - CHE - 461
Industrial Production and Utilization of EnzymesCost of PurificationThe effect of number of steps on the yield and costs in a typical enzyme purification process. For example,Step Specific activity Unit/mg 1 1 2 3 4 5 3 9 27 81 243 Cost per weigh
Air Force Academy - CHE - 461
Basics of Molecular BiologyCentral Dogma: - DNA replication - Transcription - Translation Metabolic regulation: - Genetic level - Metabolic pathway control - Cell receptorCentral DogmaCentral Dogma: universal molecule. is stored on the DNA: The
Air Force Academy - CHE - 461
Basics of Molecular BiologyCentral Dogma: - DNA replication - Transcription - Translation Metabolic regulation: - Genetic level - Metabolic pathway control - Cell receptorCentral DogmaCentral Dogma: universal - Genetic information is stored on th
Air Force Academy - CHE - 461
Metabolic Regulation. : - Enzyme activity - Cell surface receptorsMetabolic RegulationGenetic Level Regulation: Control which protein is synthesized through adjusting the rate of transcription of that gene: Feedback repression: The of enzymatic a
Air Force Academy - CHE - 461
Metabolic Regulation- Genetic level - Cellular level: - Enzyme activity - Cell surface receptorsMetabolic RegulationGenetic Level Regulation: Control which protein is synthesized through adjusting the rate of transcription of that gene: Feedback
Air Force Academy - CHE - 461
Metabolic Pathways- Overview of metabolism pathways - Catabolism - Anabolism - Bioenergetics - Important metabolic pathways - Catabolism: - Glucose catabolism - aerobic pathway - anaerobic pathway - Hydrocarbon - Nitrogen compounds - Anabolism: - Ph
Air Force Academy - CHE - 461
Cell Growth Kinetics- Introduction - Growth patterns and kinetics in batch culture - growth phases - effect of factors: oxygen supply - heat generation - Growth kinetics (Monod Equation) - Growth in continuous culture (ideal chemostat)Growth Kinet
Air Force Academy - CHE - 461
Cell Growth Kinetics- Introduction - Growth patterns and kinetics in batch culture - growth phases - effect of factors: oxygen supply - heat generation - Growth kinetics (Monod Equation) - Growth in continuous culture (ideal chemostat)Growth Kinet
Air Force Academy - CHE - 461
Batch Growth KineticsEffect of factors: aerobic growth is more efficient.- Dissolved oxygen (DO) - aerobic fermentation requires oxygen - oxygen gas is sparingly soluble in water - specific growth rate may be limited by DO if DO is below a critical
Air Force Academy - CHE - 461
Batch Growth KineticsEffect of factors: aerobic growth is more efficient.- Dissolved oxygen (DO) - aerobic fermentation requires oxygen - oxygen gas is sparingly soluble in water - specific growth rate may be limited by DO if DO is below a critical
Air Force Academy - CHE - 461
Quantifying Growth KineticsMonod equation: unstructured and nonsegregation model Unstructured model: assuming Applicable to balanced-growth condition: - exponential growth phase in batch culture - single-stage, steady state continuous culture - cel
Air Force Academy - CHE - 461
Quantifying Growth KineticsMonod equation: unstructured and nonsegregation model Unstructured model: assuming fixed cell composition. Applicable to balanced-growth condition: - exponential growth phase in batch culture - single-stage, steady state
Air Force Academy - CHE - 461
Cells Growth in Continuous CultureContinuous culture: fresh nutrient medium is continually supplied to a well-stirred culture and products and cells are simultaneously withdrawn. At steady state, concentrations of cells, products and substrates are
Air Force Academy - CHE - 461
Ideal ChemostatX0=0, kd=0, qp=0Um= D 0 0.03 0.05 0.08 0.1 0.13 0.15 0.18 0.19 0.19 S 0 0.03 0.07 0.12 0.2 0.33 0.6 1.4 3.8 4.8 0.2 hr-1 Ks 0.2 g/l Yx/s 0.41/D 1/S #DIV/0! #DIV/0! 40 35 20 15 13.33 8.33 10 5 8 3 6.67 1.67 5.71 0.71 5.26 0.26 5.21 0
Air Force Academy - CHE - 461
Ideal ChemostatX0=0, kd=0, qp=0Determine Ymax, Kd S0 D (h -1) 0.1 0.2 0.3 0.4 0.5 0.6 0.7 800 mg/L X0=0 Yx/s, max Y, app 1/Y, app 1/D 0.47 2.14 0.53 1.88 0.55 1.82 0.58 1.73 0.59 1.7 0.6 1.68 0.6 1.66 0.63S (mg/L) X (mg cells/L) 16.7 366 33.5 407
Air Force Academy - CHE - 461
Air Force Academy - CHE - 461
1. A 10-L medium containing a vitamin is to be sterilized. Assume that the number of spores initially present is 105/l. The values of the activation energy E0d for spores destruction and the pre-Arrhenius constant are E0d = 65 kcal/g-mol =1036 min-
Air Force Academy - CHE - 461
Air Force Academy - CHE - 461
Industrial Production of Citric AcidApplication of Citric Acid: - Acidulant in food, confectionary, and beverage (75%) - Pharmaceutical (10%), e.g. soluble aspirin preparation - industrial (15%): complexes with metals such as iron and copper to be s
Air Force Academy - ME - 335
ME 335 Fluid Mechanics II (January 2009)Department of Mechanical Engineering University of Saskatchewan Instructor David Sumner, Ph.D., P.Eng. Associate Professor Department of Mechanical EngineeringTelephone: E-Mail: Office:(306) 966-5537 david
Air Force Academy - CHE - 333
VISCOMETRYIntroduction This experiment involves the use of a cone and plate viscometer. You will be asked to characterize a fluid which may or may not be Newtonian. Newtonian fluids should be tested at different shear rates for a range of temperatur
Air Force Academy - CHE - 333
Natural Convection and RadiationIntroduction In this experiment heat is transmitted in parallel by two different mechanisms, free (or natural) convection and thermal radiation. We are able to distinguish between them because the rate of thermal radi
Air Force Academy - CHE - 333
ION EXCHANGE IN WATER SOFTENINGObjective: Determine the exchange capacity of a cationic resin in water softening. Introduction: Water softening is a process to reduce hardness in water and prevent the build-up of lime scale and calcium deposits in p
Air Force Academy - CHE - 333
APPENDIX1. Heat Transfer Review 2. Schematic diagram of Boiling Heat Transfer Unit: Armfield H655 3. Typical Data for Air/Water Double Pipe Heat Exchanger: Unicol 185/73 4. Shell and Tube Heat Exchanger Dimensions/Configurations 5. Flow diagram of W
Air Force Academy - CE - 468
Chapter 41LINERSCE 468 January, 2003Liners4.1 INTRODUCTION Waste Placement and Stabilization2Dry PlacementWet Placement1Liners10.1 INTRODUCTION3Liners10.2 OBJECTIVES42Liners4.3 SUBGRADE PREPARATIONIdeally, foundation lay
Air Force Academy - ME - 316
ME 3162008/2009 yearLecture note 1Lecture Note (1) Kinematics of a rigid bodyTable of contents: 1. Basic concepts 2. Classification of problems 3. The scope of problem solving using the equation in the GE226 course1. Basic conceptsWe begin
Air Force Academy - ME - 316
Figure 1But these moving coordinates are not shown on Figure 1 in order to avoid some visual difficulty.For the above two situations, we can also directly apply the equation of the angular acceleration for the three consecutive rotations, namely
Air Force Academy - ME - 316
ME 316Lecture note 4Lecture Note (4) Kinematics of Spatial Mechanisms (II)Please read this document together with "Lecture Note 4 Appendix"1. IntroductionOur previous discussion documented in Lecture Note 3 only concerned the spatial rotation
Air Force Academy - ME - 316
Appendix A for Lecture Note 4
Air Force Academy - ME - 316
ME 316Lecture note 5Lecture Note (5) Kinetics of Spatial Mechanisms: Inertia The objectivesReview the kinetics of planar mechanisms Discuss new things in the kinetics of spatial mechanismsKinetics ProblemsWhat is the cause for an object to mo
Air Force Academy - ME - 316
ME 316Lecture note 6Lecture Note (6): Kinetics of Spatial Mechanisms: Kinetics Equation ObjectivesKinetics equations Problem solving procedure Example analysisKinetics EquationWith respect to the center of gravity G of a body system, we have
Air Force Academy - ME - 316
ME 316 Dynamics and Vibration http:/engrwww.usask.ca/classes/ME/316/index.html Fall, 2008 Instructor: Prof. Chris W.J. Zhang Room 2B34 Phone: 966 5478 / Cell: 8804220 Chris.Zhang@Usask.Ca Office hours: Tuesday 11:30 1:00 Thursday 11:30 1:00 You can
Air Force Academy - ME - 316
Kinematic Analysis Equation1. General MotivationFor the problem as shown below, we need a new method.Type 5Type 4ME 316 handout 3 1Kinematic Analysis Equation2. PreliminaryProperty: If we haveA, A=constantthen we havedA = A dtAn
Air Force Academy - ME - 316
Spatial Motion: Kinematics Topics: Spatial motion and its construction Euler's theorem Equation for Euler's theoremME 316 Handout 41Spatial Motion: KinematicsA general spatial motion: a translation with a point + a rotation about a pointT
Air Force Academy - ME - 316
Kinematics of Spatial Mechanism: ClassificationTopics:Classification of problems through examplesME 316 Handout 51General equations:u r r drB / A VB = VA + u B / A + AB r B / A dta B =a A d 2 rB / A drB / A + u B / A +2AB uB/ A dt dt
Air Force Academy - ME - 316
Kinetics (I)1. Review of Kinetics of Planar Mechanisms - Inertia (mass and moment of inertia) - Governing equation (Newtons Law) 2. Moment/Product of Inertia spatial rotationME 316 Lecture 61Review of Planar Kinetics of a Rigid BodyKinetics:
Air Force Academy - ME - 316
Kinetics (II)1. Kinetics Equations 2. Classification of ProblemsME 316 Lecture 71Moment Equation for Rigid Body for Center of Gravity of the BodyZ G Y XForce equation F = maME 316 Lecture 7G2Moment Equation for Rigid Body for any fi
Air Force Academy - ME - 316
Vibrations Objectives:1. What is called vibration ? 2. Analysis 3. DesignME 316 Lecture 8 1Vibrations What ? 1. IntroductionExample 1: Equilibrium position A displaced position K: stiffnessME 316 Lecture 82Vibrations What ? Stiffness: a
Air Force Academy - ME - 316
ME 316 Handout Nr. 8ME 316 Handout Nr. 8ME 316 Handout Nr. 8ME 316 Handout Nr. 8ME 316 Handout Nr. 8ME 316 Handout Nr. 8ME 316 Handout Nr. 8ME 316 Handout Nr. 8ME 316 Handout Nr. 8ME 316 Handout Nr. 8ME 316 Handout Nr. 8Damped a
Air Force Academy - ME - 323
ME 323.3 - Winter 2009 Assignment #11.At a particular point in a loaded machine part, there is a state of plane stress in the x-y plane given by[] = 100 50 MPa . 50 200The part is made of a ductile material for which the yield stress is
Air Force Academy - ME - 323
Air Force Academy - ME - 323
ME 323.3 - Winter 2009 Assignment #21.A thin walled pipe has closed ends and is subjected to an internal pressure P.=.2.00.MPa, a resultant torque T = 7.00 kNm, and a resultant bending moment M.=.5.00.kNm. The pipe has inner diameter di = 200 mm,
Air Force Academy - ME - 323
Air Force Academy - ME - 323
ME 323.3 - Winter 2009 Assignment #3Problems 5.72, 5.75(a), 5.76All from Budynas, 2nd Edition (See Attached Pages)For Problem 5.72, some of the final answers are as follows: p = 1990 psi, For the inner cylinder, (u)r=1 = -3.5410-4 inches, (u)r=2
Air Force Academy - ME - 323
Air Force Academy - ME - 323
ME 323.3 - Winter 2009 Assignment #4Problems 6.11, 6.31, 6.37, 6.3All from Beer, Johnston Jr., and De Wolf, 4nd Edition (See Attached Pages)For Problem 6.11, the final answers are 17.63 MPa, 13.01 MPa. For Problem 6.31, the final answer is 10.79
Air Force Academy - ME - 323
ME 323.3 - Winter 2009 Assignment #5Problems 5.23, 5.26, 5.27 5.31All from Budynas, 2nd Edition (See Attached Pages)For Problem 5.26, the final answer is e = 0. Clearly explain in your solution why this is so. The final answers for Problems 5.23