2: Experiment Two step synthesis of ethyl 4-methoxycinnamate Background Why is ethyl 4-methoxycinnamate (1) interesting? First, it is the analog of octyl 4-methoxycinnamate (2), a UV light blocker found in Bain de Soleil All Day Sunblock and Coppertone Sport. The derivatives of 4-methoxycinnamic acid absorb UVB radiation due to their high level of conjugation. They are also oil soluble. UVB radiation promotes dermal cell DNA damage causing skin cancer. Octyl 4-methoxycinnamate is used in sunscreen over ethyl 4-methoxycinnamate because of its higher molar absorptivity and its greater lipophilicity.
O O R
O
R = -CH3CH3 ethyl 4-methoxycinnamate (1)
R= octyl 4-methoxycinnamate (2)
Figure 1: Derivatives of 4-methoxycinnamic acid Second, a two step synthesis is put forth so that you can practice multi-step synthesis using a variety of laboratory techniques before diving into Honors Cup. Step 1 is the Verley-Doebner modification of the Knoevenagel condensation.1 The Knoevenagel condensation reaction dates from 1896 and is a facile one-step method of forming new carboncarbon bonds under mild reaction conditions. It utilizes a weak amine base such as piperidine to initially form an enolate anion derived from a 1,3-dicarbonyl compound. The enolate subsequently acts as a nucleophile in the condensation with another carbonyl compound. This carbonyl is often an aldehyde and the reaction mechanism bears striking similarity to that of the aldol condensation. The isolated product is generally an ,-conjugated carboxylic acid, formed via ready dehydration of the intermediate -hydroxydicarbonyl compound and subsequent saponification and decarboxylation.
1
Jones, G. In Organic Reactions; Wiley: New York, 1967, pp 204-599.
Figure 2: A Generalized Knoevenagel Condensation The Verley-Doebner modification of this condensation employs pyridine as solvent and catalyst and a cocatalyst such as -alanine. With this procedure malonic acid itself, rather than its diester can be efficiently condensed with 4-methoxybenzaldehyde (p-anisaldehyde) to generate 4methoxycinnamic acid. Experimental 4-Methoxycinnamic acid [3-(4-methoxyphenyl)-2-proenoic acid] (4): In a 25 mL round bottomed flask, 4-methoxybenzaldehyde (0.804 mL, 6.61 mmol) (3), malonic acid (1.75g, 16.8 mmol), and -alanine (0.1g, 1.12 mmol) were in dissolved pyridine (3 mL, 37.1 mmol) and heated under reflux for 90 minutes. After cooling to room temperature, the reaction mixture was placed in an ice bath and 8 mL of concentrated HCl was slowly added. The resulting white precipitate was collected by vacuum filtration, washed with cold water (2x10mL) and dried thoroughly. The product was recrystallized from absolute ethanol (~20 mL). 1H NMR (200 MHz, CDCl3) 7.75 (d, 1H, J = 16.0 Hz), 7.51 (d, 2H, J = 8.8 Hz), 6.92 (d, 2H, J = 8.8 Hz), 6.33 (d, 1H, J = 15.8 Hz), 3.85 (s, 3H). Hint: For best results dry your product thoroughly by spreading it out on a watch glass/filter paper between lab days. ethyl 4-methoxycinnamate [(e)-ethyl 3-(4-methoxyphenyl)-2-propenate (1): 4 (0.6 g, 3.36 mmol) was dissolved in 10 mL of dry N,N-dimethylformamide (DMF) in a 25-mL round bottomed flask. Cesium carbonate (1.65 g, 5.06 mmol) was added followed by iodoethane (1 mL, 12.5 mmol). The flask was capped (rubber septum) and the heterogeneous mixture stirred vigorously at 50oC for one hour. After this time, HCl (1 M, 4 mL) was added to quench the reaction. The liquid was decanted from any remaining solid and extracted with 3:1 hexanes/ethyl acetate (2x10 mL). The organic layer was washed with brine (20 mL), dried with MgSO4, filtered, and solvent removed. The remaining oil solidified on standing to form colorless prisms. 1H NMR (20...