of Separation Phenols by Gas Chromatography
Objective Qualitative identification of phenols by retention time. Quantization using an internal standard. Investigation of effect of temperature and flow rate on chromatographic separation. Overview The company will prepare a standard mixture of three phenolic compounds already proven to be amenable to separation by gas chromatography. A chromatogram of the standard mixture and the associated chromatographic conditions will be provided for reference. The company is to duplicate the separation, investigate the effects of temperature and carrier flow and develop a quantitative determination using the internal standard method. An analysis will be performed on an unknown sample prepared by another company. Each member of the company is expected to understand each step of the process. It is the job of Manager to facilitate this information transfer within the company. Instrumentation Shimadzu GC-2010 equipped with a 2010 FID detector. Computer Software Shimadzu GC Solutions software Standard Mixture Phenol (the analyte) Naphthalene (the internal 1,4,6-trimethyl standard) phenol 2,3,5-trimethyl phenol 9752 g/ml in methanol 9253 g/ml in methanol 9253 g/ml in methanol 10579 g/ml in methanol
Unknown Mixture Phenol (the analyte) Naphthalene (the internal standard) 9752 g/ml in methanol 3,5-dimethyl phenol in methanol 4-t-butyl phenol in methanol
Your Assignment Replicate the separation of the standard mixture. Calculate the internal response factor (IRF). Determine The best oven temperature for the isothermal separation of the unknown mixture. Prepare separate solutions of naphthalene and phenol (~10 mg/ml) in methanol. Use these standards to determine which peaks in the unknown mixture corre...