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Review questions.2009

Course: FOS 4311, Spring 2008
School: University of Florida
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questions Review (Carbohydrates) 1) What are polysaccharides 2) What are the individual units of polysaccharides called? 3) What binds various monosaccharide units together (in a polysaccharide) What kind of bond is it? 4) What are homo-polysaccharides? Give an example 5) What are hetero-polysaccharides? Give an example 6) Name three sources of polysaccharides in nature (from plant world) 7) What are starch...

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questions Review (Carbohydrates) 1) What are polysaccharides 2) What are the individual units of polysaccharides called? 3) What binds various monosaccharide units together (in a polysaccharide) What kind of bond is it? 4) What are homo-polysaccharides? Give an example 5) What are hetero-polysaccharides? Give an example 6) Name three sources of polysaccharides in nature (from plant world) 7) What are starch granules? 8) Name 3 uses of starch in food industry? 9) What are amyloses made of? 10) What are amylopectin made of? 11) What kinds of linkages are present in amylose and amylopectin? 12) What is the structure of amylose and amylopectin? 13) Name 4 differences between amylose and amylopectin? 14) Starch granules are all of the same size True or false? 15) What kind of rice is used for making sushi? Why? (name 3 properties) 16) Starch granules are soluble in _______ water 17) What kind of chemical bonds holds amylose molecules (or amylopectin) together in a starch granule? 18) Wh...
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University of Florida - FOS - 4311
ProteinsProteins basic conceptsRole of proteins1. NutritionEnergy and essential amino acids May cause allergies and be toxic/carcinogenic Provide structure in living organisms and also foods Enzymes (which are proteins) catalyze chemical re
University of Florida - FOS - 4321
Welcome Back1Don't forget your library assignmentRead Chapter 1-3, 42Ch. 4: Evaluation of Analytical Data3The administrative processR&D ManagerLab managerMr. AMs. BMs. CMr. D4The Analytical ProcessFoodSamplingInstrum
University of Florida - FOS - 4321
PROBLEM SOLVING IN FOOD ANALYSIS Part I: DIMENSIONS AND UNITS The ultimate intention of a quantitative analysis is to reveal quantitative properties and to be able to compare the results of one assay with those of another. Expression of these results
University of Florida - FOS - 4321
FOS4321-Food AnalysisPart II: SOLUTIONSThis section is very important. It is the basis of analytical problem solving. A solution is a homogenous mixture of one substance with another, whether gas, liquid, or solid. For practical purposes, solution
University of Florida - FOS - 4321
Part I 1. 100 lbs. x 16 oz. = 1,600 oz. 1 lb. 100 lbs. x 454 g = 45,400 g 1 lb. 100 lbs. x .454 kg = 45.4 kg 1 lb. 2. 62 in. x .025 m = 1.55 m 1 in. BMI = 45.4 kg = 18.9 kg/m2 (1.55 m)2 3. 100 m x 60 sec. = 619 m/min. 9.69 sec. 1 min. 100 m x 1 mi. x
University of Florida - FOS - 4321
FOS4321-Food AnalysisPart III: Molarity and NormalityMolarity: or molar concentration denotes the number of moles of a given substance per liter of solution. The unit is mol/L and can be abbreviated with a capital letter M.Mole =M=ie Mw=98.07
University of Florida - FOS - 4321
What are the two words describing the reliability of your analytical data?A PWhat are the words describing the performance of your analytical method ?L L S S1Quantifying With External StandardPure caffeine Dissolve in 40% methanolCoffee or
University of Florida - FOS - 4321
The performance of the method Limit of detection and quantification Sensitivity Specificity Recovery rate of the spiked standard Availability of instruments and reagents Cost and speed Compliance to government regulation (official methods)
University of Florida - FOS - 4321
Name four non-random sampling methodJ R C QName four random sampling methodS S S C1The tolerance level of risk Consumer risk The risk to accept defective products; 5% or (<0.05) is often used for nonfatal risks. It translate to 95% confiden
University of Florida - FOS - 4321
1Ch. 13 pH and Titratable Acidity2The confusing concentration units Percentage % (w/w) 20 g sucrose + 80 g water 20% (w/w) % (w/ v) 20 g sucrose in 100 mL solution 20% (w/v) % (v/v) 50 mL alcohol in 100 mL solution 50% (v/v)3Dimensio
University of Florida - FOS - 4321
Organic acids in foodCOOH HO COOH COOHHO HOCOOH COOHHOCOOH COOHCOOH HO CH3Citric acid 3 COOH 1M=3NTartaric acid 2 COOH 1M=2NMalic acid 2 COOH 1M=2NLactic acid 1 COOH 1 M= 1 N1Neutralize acids with NaOHCOOH HO CH3 COONa+NaOH
University of Florida - FOS - 4321
What is the relationship between moisture and total solids? Moisture + Total Solids = % (wet basis) Why do we need to know moisture content Economics (Water = Money) Shelf life (gained and lost water) Efficiency of processing (dry beans, soups etc)
University of Florida - FOS - 4321
Ready for the first EXAM ?My.its getting hot in here.really, really hot ! ASH is the residue remaining in a food after complete combustion (oxidation). What is in foods that won't burn ? How Hot? Total ash determinations may range from 400 to 70
University of Florida - FOS - 4321
thChapter 1-3 Exam I: Monday, September 29 Chapter 4-7 Chapter 13We will have an overview on 9/26 Library assignment due Noon of Monday, September 29th22: Basic Spectroscopy 23: UV/Vis and fluorescence Production, measurement, and interpret
University of Florida - FOS - 4321
Use same method on same sample Inter-lab variation Different lab, different operator Single-lab variation Same lab, different operator Same operator, different day Same operator, same day Inter-person variation Inter-assay variation Intra-assa
University of Florida - FOS - 4321
HPLCGas ChromatographyModes of Separation: 1. Partition - classic gas or liquid chromatography 2. Adsorption - bulk binding of compounds to a solid support (I.e. Alumina, silica, carbon, etc.) 3. Gel filtration - (Size exclusion) 4. Ion Exchange
University of Florida - FOS - 4321
Gas Chromtogr aphyModes of Separation: 1. Partition - classic gas or liquid chromatography 2. Adsorption - bulk binding of compounds to a solid support (I.e. Alumina, silica, carbon, etc.) 3. Gel filtration - (Size exclusion) 4. Ion Exchange - bind
University of Florida - FOS - 4321
HPLC ChromatographyChromatography A diverse and important group of methods that permit separation of closely related components in complex mixtures A powerful separation tools Qualitative (preparative, purification) QuantitativeChromatography
University of Florida - FOS - 4321
DerivatizationWhy use Derivatization? Change property of analyte for separation Less peak tailing more stable more volatile Change property of analyte for better detection change absorbance add chromophore add halogen (for electron capture
University of Florida - FOS - 4321
CHAPTER 9: PROTEIN ANALYSISCHAPTER 9: PROTEIN ANALYSIS Why are we interested in the overall protein content of a food? Consumer says: Protein is associated with nutritional quality; inverse association between protein and fats; more protein means
University of Florida - FOS - 4321
Protein AnalysisChapter 9Other Common Protein MethodsBiuret Method Cupric ions react with peptide bonds under alkaline conditions producing a violet-purplish color (copper sulfate + K-Na-tartrate + NaOH) Measure color in SPEC at 540 nmO H
University of Florida - FOS - 4321
Do you want to separate proteins for commercial reasons or research? Take advantage of: protein solubility, size, charge, adsorption characteristics, and logical affinities for other molecules for separation. Usually will combine methods to signif
University of Florida - FOS - 6355
Part IIReview: ResolutionRs = (-1/) (k/k+1) NThe effect on Rs of:increasing ? increasing k? increasing N?H - Theoretical Plate HeightH = A + B/u + (Cs + Cm) uu = the average linear mobile phase velocity A B/u Cs Cm is a term expressing mult
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS INTRODUCTION TO CHROMATOGRAPHY - LECTURE 11 The van Deemter equation shows the relationship between the height of a theoretical plate and mobile phase velocity and other column parameters. This equation is import
University of Florida - FOS - 6355
Derivatizations In SeparationsWhat is Derivatization? Reaction Reagents Changechemical nature analyte Improve analysisWhat is Derivatization? Typically focuses on analyte Matrix ideally should remain unaffected Occasionally get some c
University of Florida - FOS - 6355
THIN LAYER CHROMATOGRAPHYTLCThin layer chromatography (TLC) is a simple, inexpensive method which requires a minimum of instrumentation and can be used for separation of simple mixtures. It is possible to separate up to 70 samples and standards on
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS THIN LAYER, SIZE EXCLUSION, CHIRAL, & AFFINITY CHROMATOGRAPHY LECTURE 13 Thin layer chromatography Thin layer chromatography (TLC) is a simple, inexpensive method which requires a minimum of instrumentation and c
University of Florida - FOS - 6355
Modes of HPLCAdsorption Chromatography is another name for liquid-solid chromatography. The solid phase is usually silica or alumina, which have highly polar surfaces. The mobile phases are commonly some of the less polar solvents. Normal-Phase Chro
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS INTRODUCTION TO HIGH PERFORMANCE LIQUID CHROMATOGRAPHY LECTURE 14 Modes of HPLC Adsorption Chromatography is another name for liquid-solid chromatography. The solid phase is usually silica or alumina, which have
University of Florida - FOS - 6355
UV DetectorsFixed Wavelength Variable Wavelength Diode arrayFixed WavelengthMercury vapor lamps are probably the most common and emit intense light at 253.7 nm (and certain other wavelengths). Because of the limited emission spectra, of the lamp
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS DETECTORS FOR HPLC - LECTURE 15UV VIS Spectroscopy There are three types of UV detectors, fixed wavelength, variable wavelength and diode array. The fixed types use a lamp which emits at a certain wavelength. M
University of Florida - FOS - 6355
Electrophoresis is the movement of electrically charged particles through a media caused by an electric current.A high voltage, up to 30 kV is applied causing positively charged cations to migrate towards the negative electrode (cathode) and negativ
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS CAPILLARY ELECTROPHORESIS - LECTURE 16 Electrophoresis is the movement of electrically charged particles through a media such as a buffer system caused by an electric current. An electrophoresis system consists o
University of Florida - FOS - 6355
Normal-phase chromatographyNormal-phase chromatography is really not that normal. That is to say that it is used much less frequently than reversed-phase chromatography. The main reason for this is simply that many separations can be accomplished us
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS NORMAL-PHASE CHROMATOGRAPHY - LECTURE 17 Normal-phase chromatography is really not that normal. That is to say that it is used much less frequently than reversed-phase chromatography. The main reason for this is
University of Florida - FOS - 6355
Reversed-Phase is the Most Common Type of HPLC- Columns are rugged, efficient, reproducible, and stable. - Aqueous mobile phases are less expensive then organic phases. - Less time is required for equilibration of the column between runs. - Detectio
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS REVERSED PHASE CHROMATOGRAPHY - LECTURE 18Reversed-Phase for Non-Ionic Analytes Reversed-phase is the most common type of HPLC. Reversed-phase columns are rugged, efficient, reproducible, and stable. Aqueous mo
University of Florida - FOS - 6355
Ion-Pair ChromatographyIn addition to the aqueous buffer and an organic solvent that is typical for reversed-phase, the mobile phase contains a counter ion that combines with the analyte ions to form an ion pair. This forms a neutral species that ca
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS CHROMATOGRAPHY OF IONIC COMPOUNDS - LECTURE 19Ion-Pair Chromatography Ion-pair is a type of reversed-phase chromatography that has been adapted for the separation of ionic compounds. In this method, the mobile
University of Florida - FOS - 6355
Sample InjectionFigure 1. Influence of sample solvent on peak shape. The sample is dissolved in buffer with (a) 0%, (b) 30%, (c) 50%, and (d) 70% acetonitrile. (Mobile Phase 92% buffer pH 3.5, 8% acetonitrile)Column TestingThe best way to evalua
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS TROUBLESHOOTING HPLC SYSTEMS - LECTURE 20 Sample Solvent Strength The extracted sample is usually dissolved in a solvent that is best for the extraction. This sample solvent may not be compatible with the mobile
University of Florida - FOS - 6355
Gas ChromatographyInjector Port Flow ControlDetectorRecorderRecorderColumnColumn Oven Carrier Gas The GC system consists of gas supplies for the mobile phase (and the detector, if needed), flow controls for the gasses, an injector, an oven
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS INTRODUCTION TO GAS CHROMATOGRAPHY - LECTURE 21 Sample Preparation and Injection Gas chromatography (GC) is a commonly used method of separating components of a complex mixture. The GC system consists of gas supp
University of Florida - FOS - 6355
Capillary ColumnsThe geometry of capillary columns is fairly simple, consisting of length, internal diameter, and stationary phase thickness. Nevertheless, there are endless possible combinations of these three factors that could be used for optimiz
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS GAS CHROMATOGRAPHY - LECTURE 20 Mobile and Stationary Phases Columns for Gas Chromatography Capillary columns are commonly made from high purity silica which contains no significant metallic oxides that may react
University of Florida - FOS - 6355
Flame Ionization DetectorMost common detector Carbon atoms (C-C bonds) are burned in a hydrogen flame. A small portion of carbon atoms are ionized (about 1 in 10,000), The ions carry a charge from the flame to the walls of the detector which surroun
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS DETECTORS FOR GAS CHROMATOGRAPHY - LECTURE 24 There are a number of detectors used in GC. Generally the detector produces a signal in proportion to the amount of analyte present. Some types are based on concentra
University of Florida - FOS - 6355
GCColumn CareColumns have a finite life, but they will last much longer if the gas chromatography system is operated with care. The major causes of reduced column life are breakage thermal damage chemical damage contaminationColumn BreakageIf t
University of Florida - FOS - 6355
INSTRUMENTAL ANALYSIS AND SEPARATIONS GAS CHROMATOGRAPHY - LECTURE 25 Maintenance and Troubleshooting Columns Columns have a finite life, but they will last much longer if the gas chromatography system is operated with care. The major causes of reduc
University of Florida - FOS - 6355
FOS 6355 Summer 2005Russell RouseffIsotope Abundances O (~99.76%) 17 O (0.04%) 18 O (0.20%) 12 C (~98.9%) 12.0107 Amu 13 C (~1.1%) 35 Cl (75.77%) and 37Cl (24.23%) 35.4527 79 Br (50.50%) and 81Br (49.50%) 79.90416High Resolution MSC6H12
Princeton - HIS - 325
HIS 325 Modern China Lecture #3 9/19/02 Population and Environmental Degradation: A Tale of Two Rivers and many people I. II. III. China's major rivers: Yangzi and Yellow Triangle relationship of Increased population, problem environment, and limited
Princeton - HIS - 325
HIS325 Modern China Fall 2002 MIDTERM Projects DUE Monday, NOV. 4th Each of the following project options are designed to get you to explore in greater depth one of the topics we have learned about in the first half of the course: treaty-ports, the B
Princeton - HIS - 325
HIS325 Modern China Lecture #4, 9/24/02 Analyzing the Opium War and "East/West" Divergences I. II. III. Ways to look at the Opium War: 1) product of British venality (focusing on opium); 2) conflict about the nature of trade; 3) clash of legal cultur
Princeton - HIS - 325
HIS325 Modern China Lecture 9/26/02 Orthodoxy and Heterodoxy, Religion and Rebellion First finish Tuesday's lecture on interpreting the Opium War: can we say the Qing had "fallen behind"? And a look at questions of hierarchy written into the Treaty o
Caltech - BIB - 1976
Caltech - BIB - 1976
Caltech - BIB - 1976
Caltech - BIB - 1976
Commun. math. Phys. 49, 187-190 (1976)Communications inMathematical Physics by Springer-Verlag 1976The Existence of Maximal Slicings in Asymptotically Flat SpacetimesM. Cantor*, A. Fischer* *, J. Marsden + *% N. G Murchadha + +, and J. York
Caltech - BIB - 1976
Caltech - BIB - 1976
Caltech - BIB - 1983