Molecular Genetics Bio350/516 Lecture 8, 2/14/06 Today I. Return Exams II. Chapter 6 (Prokaryotic Transcription, continued) A. The role of the subunit in UP promoter recognition B. Which subunit of RNAP core encodes RifR? C. Which subunit of RNAP core contains the active site for <a href="/keyword/phosphodiester-bond/" >phosphodiester bond</a> formation? D. How big is the RNA-DNA hybrid? The Role of the Subunit in Binding to the UP Element Figure 5.36a Fingerprinting using DNase Figure 6.28 Figure 6.29 Which Subunit Encodes RifR? Figure 6.31 Which Subunit Contains the Active Site for <a href="/keyword/phosphodiester-bond/" >phosphodiester bond</a> Formation? Figure 6.32 Affinity Labeling for Labeling the Site of <a href="/keyword/phosphodiester-bond/" >phosphodiester bond</a> Formation. This is an analog of ATP This is radioactive UTP Which Subunit Contained the Radioactivity? Figure 6.33 Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Figure 6.35 and Hydrophobic binding Electrostatic Binding How Big (i.e., how many bp) is the RNA-DNA Hybrid? Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Figure 6.37a -1 3 5 Radioactively end labeled U is at the -2 position on the mRNA. Now crosslink to DNA an...