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Berkeley - MCB - 130
MCB 130 R. Schekman Membrane Structure Membrane Variety and Molecular Constituents A. Cells have membrane-bounded compartmentsLectures 1, 21. Membranes revealed by electron microscopy and by isolation of specialized organelles 2. Membranes provi
Berkeley - MCB - 130
MCB 130 R. Schekman Membrane Structure Asymmetry, Protein Mobility A. Evaluation of protein and lipid orientation in bilayerLectures 2, 31. Lectin binding indicates glycoproteins are asymmetrically oriented. 2. Proteolytic mapping demonstrates a
Berkeley - MCB - 130
MCB130 R. Schekman Transport of Small Molecules A. Function and types of permeaseLectures 4, 51. Permeases govern: ionic composition of cells and organelles; membrane potential; nutrient (sugar, amino acid) concentration. 2. Some permeases act to
Berkeley - MCB - 130
MCB130 R. Schekman Membrane Transport: Nucleocytoplasmic Transport I. Nuclear envelope A. Nuclear membraneLectures 5, 61. Pores are large structures that span junction of inner and outer membrane. 2. Transport of particles bi-directionally throug
Berkeley - MCB - 130
MCB130 R. Schekman Membrane Assembly: Signal Hypothesis and Translocation Machinery A. Signal hypothesisLectures 6, 71. Proteins destined for secretion or assembly in plasma membrane originate on ribosomes attached to endoplasmic reticulum (ER).
Berkeley - MCB - 130
MCB130 R. SchekmanLectures 8, 9Membrane Vesicular TrafficI. Transport from the endoplasmic reticulum A. Sorting of secretory and resident proteins 1. Proteins destined for transport are packaged into transport vesicles by coat proteins called C
Berkeley - MCB - 130
MCB130 R. SchekmanLectures 9, 10Membrane Traffic to the Lysosome and Cholesterol RegulationA. Transport to the lysosome 1. Lysosomes in animal cells serve to turn over cell macromolecules by hydrolysis. a. Hydrolytic enzymes must be specificall
Berkeley - MCB - 130
MCB130 R. Schekman Action Potential, Synaptic Transmission and Membrane Fusion A. Action potentialLectures 11-141. Because of the Na+ , K+ ATPase, nerve cells have a high external [Na+ ] 440 mM relative to cytoplasmic concentration (50 mM) and c
Berkeley - MCB - 130
MCB130, Midterm 1 Fall `05 Signature: SID:Name: GSI:Use only the space provided below to answer each question. Do not write on the back. For maximal credit, leave out extraneous information. 100 points total. 1. (10pts) How are sister chromatids
Berkeley - MCB - 130
MCB130, Midterm 2 Fall "03SID:Name: GSI:Use only the space provided below to answer each question. Do not write on the back. For maximal credit, leave out extraneous information. 90 points total. 1. Assume that the critical concentration of an
Berkeley - MCB - 130
1) Rank these 3 phospholipids in order from most fluid (1) to least fluid (3), and explain your reasoning? a- palmitate (C16, saturated) is (2), because it has no double bonds and has van der Waals contacts (from the saturated carbons) that give rigi
Berkeley - MCB - 130
MCB130 week1 solutions 1. a) What does amphipathic mean? This term is used to describe molecules with both hydrophobic and hydrophilic regions. b) Aside from phospholipids, name two other amphipathic molecules we have discussed in lecture? Sphingoli
Berkeley - MCB - 130
MCB130 week 2 solutions 1) Fill out the following table. Complex Found where? Active or passive? Symport or antiport? Type of molecules transported and along (with) or against gradient H+ Along/Down gradient1Proton translocating ATPase (FType) P
Virginia Tech - SOC - 1004
IInttrroducttorry Sociiollogy llectturres n oduc o y Soc o ogy ec u es Course Intro: 1/16-Sociology as a discipline What causes human behavior? Who killed Susan Smith's kids? Frameworks for explaining human behavior provide answers. Frames of Blame L
Michigan - ECON - 101
Econ 101 Chapter 1-People make purposeful choices with scarce resources, to interact with other people when they make these choices. Economics is mostly the study of how people deal with scarcity.Vocab Scarcity A situation in which people's res
Michigan - ECON - 101
Review Session Andres Cooper 1. Externalities a. Defn: the cost of producing a good or the benefits from consuming a good spill over to those not producing or consuming the good b. Graphs (shift supply curve) i. Negative: (Mg social cost = Mg private
Virginia Tech - HD - 2314
Chapter 4: Female Sexual AnatomyThe Female Sexual and Reproductive System External Sex Organs Internal Sex Organs Other Sex OrgansExternal Sex Organs Vulva/Pudendum entire female region of external sex organs Mons Veneris protective, fatty
Berkeley - MCB - 130
MCB130 week 3 solutions 1) What is puromycin, what is it used for, and how does it work?1It detaches the nascent chain from a ribosome in the middle of translating an mRNA. It can be used to remove the transcripts from purified rough ER microsom
Berkeley - MCB - 130
MCB130 Week 4 Key 1) You have discovered a novel integral membrane protein, and you suspect it's a receptor of some sort involved in signaling between cells. The sequence of the protein suggests that it's a polytopic membrane protein with 5 anchor d
Virginia Tech - HD - 2314
Chapter 5: Male Sexual Anatomy and PhysiologyThe Male Sexual and Reproductive System External Sex Organs Internal Sex Organs Other Sex OrgansExternal Sex Organs Boys are typically more comfortable with their genitalia than girls Testes are vi
Virginia Tech - HD - 2314
Chapter 8 Childhood and Adolescent Sexuality Human Development Human Development: Stages Infancy (birth to age 2) Infancy (birth to age 2)TRUE OR FALSE? Male fetuses have erections in the uterus Females can have vaginal lubrication from birth Yo
Virginia Tech - HD - 2314
Chapter 3: Gender Development, Gender Roles, and Gender IdentityGender and Sex Sex biological aspects of being male or femaleEvidence for Nature and Nurture 1 in 2000 births involves a baby with ambiguous genitals or reproductive structures that
Berkeley - MCB - 130
MCB 130 Sections 101 and 102 Week 4 - ER and Golgi GSI: Jackie Chretien jacquelineann@berkeley.edu1. In the following protein: S = signal peptide, {} = hydrophobic region{S}1{2}3{4}5{6}7{8}9(a) Draw the predicted orientation o
Berkeley - MCB - 130
MCB 130 Sections 101 and 102 Week 6 - Imaging and Cytoskeleton intro GSI: Jackie Chretien jacquelineann@berkeley.edu1. Fill in this table about different types of imaging techniques (use + or - ) : GFP et al. live imaging high resolution labeling s
Berkeley - MCB - 130
MCB 130 Sections 101 and 102 Week 7 - The Actin Cytoskeleton GSI: Jackie Chretien jacquelineann@berkeley.edu1. Draw cartoons of the three phases of actin assembly. Include kinetics arrows, indicate whether the actin is ATP, ADP+Pi, or ADP bound, and
Berkeley - MCB - 130
MCB 102 Handout 9/6 CHAPTER 1: FOUNDATIONS OF BIOCHEMISTRY Three domains of life: Eukaryotes-nuclear envelope, includes animals, plants and fungi Eubacteria-no nuclear envelope, includes bacteria such as E. coli Archaebacteria-no nuclear envelope, in
Berkeley - MCB - 130
MCB102 Handout 8/30 The following is an amino acid in its fully protonated form: H H O HN+CCOH H H 1a. Circle the alpha carbon. Put a box around the R group. Which amino acid is this? What are its three letter and one letter abbreviations?1b.
Berkeley - MCB - 102
MCB102 Handout 9/13 Levels of protein structure and the most significant interactions stabilizing them Primary-sequence of amino acid residues from N terminus to C terminus, covalent bonds Secondary-regular structure formed locally among nearby amino
Berkeley - MCB - 102
1.Draw the approximate positions of these four proteins on a 2D gel. molecular weight = 16,700 Da pI = 7.0 molecular weight = 14,400 Da pI = 11.0 molecular weight = 34,000 Da pI < 1.0 molecular weight = 480,000 Da pI = 5.0 Decreasing pIMCB102 Han
Berkeley - MCB - 102
MCB102 Handout 9/20 1. Draw the approximate positions of these four proteins on a 2D gel. molecular weight = 16,700 Da pI = 7.0 molecular weight = 14,400 Da pI = 11.0 molecular weight = 34,000 Da pI < 1.0 molecular weight = 480,000 Da pI = 5.0Myogl
Berkeley - MCB - 102
Glucose 6-phosphate 6-phosphoglucono-lactone Lactonase 6-phosphogluconate dehydrogenase1 2G6PNADP OH H+NADPHH2OH+PENTOSE PHOSPHATE PATHWAY C-1 C-2 C-3 C-4 of ribulose 5-phosphate C-5NADP+ NADPH + CO2 6-phosphogluconate dehydrogenas
Berkeley - MCB - 102
For each of the following pairs of half-reactions, what the net reaction and the free energy change? Fumarate + 2H+ + 2eSuccinate E' = 0.03 V FAD + 2H+ + 2eFADH2 Fumarate + FADH2 FADH2 FAD + 2H+ + 2eFAD + succinate E' = -0.22 V E' = 0.22 V E' = 0.25
Berkeley - MCB - 102
REDOX REACTIONSElectrons ALWAYS flow towards the redox pair with the HIGHER standard reduction potential, E'. To solve a redox problem: 1. Determine which half-reaction has the higher E'. 2. Reverse the other reaction and the sign of its E' value. 3
Berkeley - MCB - 102
MCB 102 Handout 10/25FATTY ACID BREAKDOWN aka OXIDATIONTriacylglycerols are stored in adipocytes until a hormone-sensitive lipase hydrolyzes them to yield glycerol and three free fatty acids. STEP 1: Linkage to CoA STEP 2: Transport into the mito
Berkeley - MCB - 102
MCB102 Handout 11/22 This handout covers lectures 5-8. You should look over the reader, your class notes, and the assigned book pages for clarification. Unless otherwise noted, the processes occur in the bacterium E. coli.REPLICATION (continued)IN
Berkeley - MCB - 102
MCB102 Handout 11/29SOUTHERN BLOTTING1. 2. 3. 4. 5. 6. 7. Digest genomic DNA with restriction enzymes Perform agarose gel electrophoresis Denature DNA with base Transfer to a membrane Incubate the membrane with a radioactively labeled probe Wash a
Berkeley - MCB - 102
MCB102 Quiz #1 9/13 Please write your name on the back of the quiz. You may use pencil or pen. Question 1 (6 points) The following is a peptide at pH 7:H H O H O H O H O H OHOH N+ C C N C C N C C N
Berkeley - MCB - 102
MCB102 Quiz #1 9/13 Please write your name on the back of the quiz. You may use pencil or pen. Question 1 (6 points) The following is a peptide at pH 7:H H O H O H O H O H OHOH N+ C C N C C N C C N
Berkeley - MCB - 102
MCB102 Quiz#2 9/20 Please write your name on the back of the quiz. You may use pencil or pen. Question 1 (8 points) 1a. Draw the peptide with the sequence EDR at pH 7.pKa of terminal amino group is 9.7 pKa of terminal carboxyl group is 2.2 pKa of E
Berkeley - MCB - 102
MCB102 Quiz#2 9/20 Please write your name on the back of the quiz. You may use pencil or pen. Question 1 (8 points) 1a. Draw the peptide with the sequence EDR at pH 7.pKa of terminal amino group is 9.7 pKa of terminal carboxyl group is 2.2 pKa of E
Berkeley - MCB - 102
MCB102 Quiz #4 10/25 Question 1 (11 points)pyruvateCmalateAoxaloacetateBcitrateDphosphoenolpyruvate (PEP)_The name of the enzyme that catalyzes reaction A is _malate dehydrogenase_. The electron carrier that is reduced in reaction
Berkeley - MCB - 102
MCB102 Quiz #5 11/15 Question 1 (4 points) 1a. Which base is shown below? Is it a purine or pyrimidine? Guanine, purine (2 points) -1 for wrong base -1 for pyrimidine 1b. How do magnesium ions stabilize the structure of DNA? (Maximum length: 1 senten
Berkeley - MCB - 102
MCB102 Quiz#6 11/29 Question 1 (6 points) Give the name of the E. coli enzyme that acts at each of the following steps in leading strand DNA synthesis (drawing not to scale). (Maximum length: 1 sentence each, full sentences not required)1a.What e
Kennesaw - CHEM - 1212
3805 Canton Pl. Kennesaw, GA 30144 December 19, 2007 Ms. Bridget Lemley ChemLink, Kennesaw Dear Ms. Lemley, Thank you for taking your time to consider me for employment. Please allow me to introduce myself. My name is Lauren Tankesley and I am respon
Kennesaw - CHEM - 1212
Determination of a Ka Constant Lauren Tankesley and Megan Cobb-Young Purpose: Use the process of acid-base titration to neutralize an acid through titration of the base into the acid. The data gathered determines the equilibrium constant for a given
Kennesaw - CHEM - 1212
Formal Lab ReportYour formal report should be typed neatly in paragraph form in your own words. Follow correct spelling and grammar rules. The report should be written in the past tense. (Do not tell us how we should do the experiment; tell us how y
Kennesaw - CHEM - 1212
Mixtures vs. solutions: Mixture: Heterogeneous- different phases A + B _ Solution: chemical interaction Homogeneous, single phase. *Hexane would not dissolve in water because they are too dissimilar. There are LDF and H-Bonding forces, which do not c
Kennesaw - CHEM - 1212
Question 1Chemistry: The Molecular Nature of Matter and ChangeMartin Silberberg 4th edition Chapter 20 Which of the following processes is NOT spontaneous? A) Methane burning in air. B) A teaspoon of sugar dissolving in a cup of hot coffee. C) A sa
Kennesaw - CHEM - 1212
Question 1Chemistry: The Molecular Nature of Matter and ChangeMartin Silberberg 4th edition Chapter 12 Which of the following is an intramolecular force? A) Those preventing oil from evaporating at room temperature. B) Those preventing butter from
Kennesaw - CHEM - 1212
Question 1Chemistry: The Molecular Nature of Matter and ChangeMartin Silberberg 4th edition Chapter 13 Which of the following has the member more soluble in diethyl ether listed first? A) NaCl(s), HCl(g) B) CH3CHO(l), H2O(l) C) MgBr(s), CH3CH2MgBr(
Kennesaw - CHEM - 1212
Kinetics: Rates and Mechanisms of Chemical Reactions16.1 Factors That Influence Reaction Rates 16.2 Expressing the Reaction Rate 16.3 Rate Law and Its Components 16.4 Integrated Rate Laws: Concentration Changes over Time 16.5 Effect of T on rxn rat
Kennesaw - CHEM - 3361 LAB
Lauren Tankesley 3361L-02 1. A functional group is an atom or group of atoms in a molecule responsible for reactivity under a given set of conditions 2. OH O (X=Br, Cl, I, F) | | -OH -CH=CH-C=O CH3-C-CH3 R-X Alcohol Alkene carboxylic ketone alkyl hal
Kennesaw - CHEM - 3361 LAB
Melissa Henkel Amanda? Chem 3361L/05 25 September 2007 Acid-Base Reactions: Separation of Pain Reliever Components Data:Table1: Experimentally determined amounts Amount/g Phenacetin .518g Aspirin .085g Caffeine .053gMelting points oC 106-127 107-