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Course: BIO 102, Spring 2008School: Rutgers
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this In lab the enzyme is Alkaline Phosphatase. The substrate in this lab is PNitro-Phenyl Phosphate or pNPP. The products formed by Alkaline Phosphate and PNitro-Phenyl Phosphate are p-Nitrophenol and inorganic phosphate or pNP+Pi. The purpose of this lab is to determine the optimum conditions for an enzyme catalyzed reaction. For experiment I my group predicted that Tube 3 would have the highest rate because...
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this In lab the enzyme is Alkaline Phosphatase. The substrate in this lab is PNitro-Phenyl Phosphate or pNPP. The products formed by Alkaline Phosphate and PNitro-Phenyl Phosphate are p-Nitrophenol and inorganic phosphate or pNP+Pi. The purpose of this lab is to determine the optimum conditions for an enzyme catalyzed reaction. For experiment I my group predicted that Tube 3 would have the highest rate because there were no added inhibitors. Inhibitors decrease the rate of a reaction (Solomon et al,. 2005). For experiment IIa my group predicted that Tube 1 would have the highest rate because it was the purest mixture of the enzyme. An is enzyme "an organic catalyst that accelerates a specific chemical reaction by lowering the activation energy required for that reaction" (Solomon et al,. 2005). For experiment IIb my group predicted that the rate of reaction would decrease with the concentration of the substrate pNPP. We came up with this prediction after reading the textbook (Solomon et al,. 2005). For experiment IIIa my group predicted that Tube 4 would have the highest rate because every other experiment in this lab dealt with pH 8.0. For experiment IIIb my group predicted that Tube 4+ would have the greatest product formed because heat is a stimulus.
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