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HW 8
Course: BIO 202, Fall 2007School: New Mexico
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202 Biology NAME: Fall 2007 T.A.: Homework 8 Discussion Section #: Due: Monday, October 29, by 5pm Turn in to T.A. mailbox 1. (3 pts) The human human pedigree on the next page shows people affected with the rare nail-patella syndrome (misshapen nails and kneecaps) as filled squares and circles, and gives the ABO blood group genotype of each person. The nail-patella and the ABO blood group loci are autosomal. a....
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202 Biology NAME: Fall 2007 T.A.: Homework 8 Discussion Section #: Due: Monday, October 29, by 5pm Turn in to T.A. mailbox 1. (3 pts) The human human pedigree on the next page shows people affected with the rare nail-patella syndrome (misshapen nails and kneecaps) as filled squares and circles, and gives the ABO blood group genotype of each person. The nail-patella and the ABO blood group loci are autosomal. a. Is the nail-patella syndrome dominant or recessive? Give your reasons for the answer. b. Is there evidence of linkage between the nail-patella gene and the gene for ABO blood type. c. If there is evidence of linkage, then draw a diagram showing the alleles on the relevant homologous chromosomes of the grandparents (generation 1).
d. According to your model, which descendants are recombinants? e. What is the best estimate of the recombination frequency? f. If man III-1 marries a normal woman of blood type O, what is the probability that their first child will be blood type B with a nail-patella syndrome? 2. (1 pts) B and R are linked genes in fruit flies. A fruit fly of the genotype B R/b r is testcrossed with b r/b r. In 84 percent of the meiosis, there are no chiasmata between the linked genes; in 16 percent of the meiosis, there is one chiasma between the genes. What proportion of the progeny will be B r/b r?
3. (3 pts) A Drosophila geneticist made a cross between females homozygous three for X-linked recessive mutations (y, yellow body; ec, echinus eye shape; w, white eye color) and wild type males. He then mated the F1 females to triply mutant males and obtained the following results. F2 Females + + + / y ec w y ec w / y ec w y + + / y ec w + ec w/ y ec w y + w / y ec w + ec + / y ec w + + w / y ec w y ec + / y ec w F2 Males +++ y ec w y++ + ec w y+w + ec + ++w y ec + Number 475 469 8 7 18 23 0 0
Determine the order of the three loci y, ec and w and estimate the distances between them on the linkage map of the X chromosome.
4. (3 pts) You have identified a mutant E. coli strain that cannot synthesize histidine (His-). To determine the location of this mutation on the E. coli chromosome, you perform interrupted mating experiments with five different Hfr strains. The following table shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first five markers (mutant genes) into the His- strain. Hfr A Hfr B Hfr C Hfr D Hfr E bio (4) xyl (6) his (3) xyl (7) his (4) glu (20) met (18) cys (13) thr (25) glu (11) his (27) tyr (24) tyr (21) lac (40) bio (27) cys (37) cys (32) met (27) bio (48) lac (35) tyr (45) his (42) xyl (39) glu (62) thr (50)
On the map of the circular E. coli chromosome, indicate (1) the relative location of each gene and (2) the position where the sex factor is integrated in each of the Hfr chromosome.
thr
lac
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