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Course: MCB 104, Spring 2010School: Berkeley
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AUTOSOMALDOMINANTINHERITANCE Week2Pedigrees Ifthisweretobearecessiveinheritancepattern,themutationwouldhavehadtohavebeenpresentin LynnandTimsparents,Lynnshusband,andjohnswife.Unlessthisisaverycommonmutation,a AUTOSOMALRECESSIVEINHERITANCE recessiveinheritancepatterisunlikelyinissituation. 1
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AUTOSOMALDOMINANTINHERITANCE
Week2Pedigrees Ifthisweretobearecessiveinheritancepattern,themutationwouldhavehadtohavebeenpresentin LynnandTimsparents,Lynnshusband,andjohnswife.Unlessthisisaverycommonmutation,a AUTOSOMALRECESSIVEINHERITANCE
recessiveinheritancepatterisunlikelyinissituation. 1
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Berkeley - MCB - 104
2/1/2010 MCB 104; Handout #1 1. Barbara McClintock demonstrated that genes are distributed linearly on chromosomes by demonstrating that recombination between traits correlated with recombination between knobsphysical features of maize chromosomes. Why di
Berkeley - MCB - 104
2/1/2010 MCB 104; Handout #1 1. Barbara McClintock demonstrated that genes are distributed linearly on chromosomes by demonstrating that recombination between traits correlated with recombination between knobsphysical features of maize chromosomes. Why di
Berkeley - MCB - 104
2/8/2010 MCB 104; Handout #2 1. Anterograde transport: theres not a ton of memorization in this class, but you should know the order in which a secreted protein passes through the ER, and various golgi cisternae in anterograde transport. Ribosome/Cytoplas
Berkeley - MCB - 104
2/8/2010 MCB 104; Handout #2 1. Anterograde transport: theres not a ton of memorization in this class, but you should know the order in which a secreted protein passes through the ER, and various golgi cisternae in anterograde transport. 1A. In which step
Berkeley - MCB - 104
2/15/2010 MCB 104; Handout #3 1. The Ras pathway is the canonical Receptor Tyrosine Kinase (RTK) signaling pathway. Put the following steps of Ras signaling in order (note: well very likely be talking about the Ras pathway again in the genetics section of
Berkeley - MCB - 104
2/15/2010 MCB 104; Handout #3 1. The Ras pathway is the canonical Receptor Tyrosine Kinase (RTK) signaling pathway. Put the following steps of Ras signaling in order (note: well very likely be talking about the Ras pathway again in the genetics section of
Berkeley - MCB - 104
2/22/2010 MCB 104; Handout #4 1. What are kinetochores, and where are they found? Other key terms to know: centromere, cohesin, Dam1. Stanfurd students often confuse centromeres with centrosomes. Dont make the same mistake. Kinetochores are protein comple
Berkeley - MCB - 104
2/22/2010 MCB 104; Handout #4 1. What are kinetochores, and where are they found? Other key terms to know: centromere, cohesin, Dam1. Stanfurd students often confuse centromeres with centrosomes. Dont make the same mistake.2. Which of the following pairs
Berkeley - MCB - 104
2/26/2010 MCB 104; Handout #5 1. Professor Dernburgs slides list six hallmarks of cancer cells. Know them. Sustained angiogenesis, self sufficiency in growth signals, insensitivity to antigrowth signals, tissue invasion and metastasis, limitless replicati
Berkeley - MCB - 104
2/26/2010 MCB 104; Handout #5 1. Professor Dernburgs slides list six hallmarks of cancer cells. Know them. Think about experiments you could do to see if a cell had acquired each of those hallmark properties. For example: A. You are culturing mammalian ce
Berkeley - MCB - 104
3/8/2010 MCB 104; Handout #6Answer Key1. You have a mixture of Neruospora, some of which are auxotrophs and some of which are prototrophs. How can you quickly isolate the prototrophs? How could you identify the auxotrophs? Grow them on minimal media. Pr
Berkeley - MCB - 104
3/8/2010 MCB 104; Handout #6 1. You have a mixture of Neruospora, some of which are auxotrophs and some of which are prototrophs. How can you quickly isolate the prototrophs? How could you identify the auxotrophs? 2. You are studying a haploid organism th
Berkeley - MCB - 104
3/15/2010 MCB 104; Handout #7 GUIDE TO EPISTASIS ANALYSIS: Intro: Often, genes function together in a biological pathway. Epistasis is used to determine the order of genes in that pathway. Epistasis requires making a double mutant for two genes with disti
Berkeley - MCB - 104
3/15/2010 MCB 104; Handout #7 Practice problems: 1. What will be the vulval phenotype of each of the following classes of C. elegans mutant? 1A. EGF- (null); EGFR* (constitutive): Multivulva (1o & 2o fates) 1B. EGFR- (null) Ras* (constitutive): Multivulva
Berkeley - MCB - 104
3/29/2010 MCB 104; Handout #8 1A. In Drosophila, will mutations that prevent the functioning of the dosage compensation machinery be lethal in males, females, or both? Explain. Males. Drosophila dosage compensate by increasing the expression of X-linked g
Berkeley - MCB - 104
3/29/2010 MCB 104; Handout #8 1A. In Drosophila, will mutations that prevent the functioning of the dosage compensation machinery be lethal in males, females, or both? Explain. 1B. In C. elegans, will mutations that prevent the functioning of the dosage c
Berkeley - MCB - 104
MCB 104 3/17/2010Quiz #2 25 points possibleName: SID:Note: I have included explanations to help you study. Explanations were not required to receive full credit on this quiz. All that was required was the pathway, compound, phenotype, or ratio, as requ
Berkeley - MCB - 104
MCB 104 Sections 109 & 110 Section 109 Wed 11-12pm Section 110 Wed 12-1pm156 Dwinelle 215 DwinelleGSI: Scott Siera Email: scott.siera@gmail.com Office Hours: Tue, 10-11am at Cafe Strada (@ corner of Bancroft & College) Grading: Three (3) quizzes, 25 pts
Berkeley - MCB - 167
Discussion 2: Pheromones and Olfaction MCB 167 Spring 2010. For Discussion Sections Feb 5. Overview Pheromones are odorant molecules that mediate species-specific chemical communication, including finding and recognizing a mate, recognizing kin vs. non-ki
Berkeley - MCB - 167
Vol 448 | 30 August 2007 | doi:10.1038/nature06089A RTICLESA functional circuit underlying male sexual behaviour in the female mouse brainTali Kimchi1, Jennings Xu1 & Catherine Dulac1In mice, pheromone detection is mediated by the vomeronasal organ an
Berkeley - MCB - 167
!"#$%#"&'()*(+&,&-(."#"&' /+0(123(45-"'6()7178(9&-(!"#$%#"&'(4:$;"&'(9:<(1)8 !"#$"%#& =-"$>-&?@;"$($&,&-(."#"&'(@-&#:("'(A,B(C&-,B(?&'D:E#(@'B(>%?@'#8(=>:(@BB";"&'(&F(@(;>"-B($&,&-(&5#"'( 5-&."B:B(;>:(@<",";E(;&(B"#;"'6%"#>(6-:'(F-&?(-:B8(4%<#:G%:';(,&#(&
Berkeley - MCB - 167
Vol 461 | 8 October 2009 | doi:10.1038/nature08401L ETTERSGene therapy for redgreen colour blindness in adult primatesKatherine Mancuso1, William W. Hauswirth2, Qiuhong Li2, Thomas B. Connor3, James A. Kuchenbecker1, Matthew C. Mauck3, Jay Neitz1 & Mau
Berkeley - MCB - 167
MCB167 DiscussionassignmentforFriday,Feb19 DirectionSelectivityinMammalianRetina Reading: YoshidaK,WatanabeD,IshikaneH,TachibanaM,PastanI,NakanishiS.Akeyroleofstarburstamacrine cellsinoriginatingretinaldirectionalselectivityandoptokineticeyemovement.Neur
Berkeley - MCB - 167
Neuron, Vol. 30, 771780, June, 2001, Copyright 2001 by Cell PressA Key Role of Starburst Amacrine Cells in Originating Retinal Directional Selectivity and Optokinetic Eye MovementKazumichi Yoshida,1 Dai Watanabe,1 Hiroshi Ishikane,3 Masao Tachibana,3 Ir
Berkeley - MCB - 167
MCB!167! Discussion!assignment!for!Friday,!Feb!26! Axonal!Delay!Lines!for!ITD!Calculation!! Reading!:! Seidl!AH,!Rubel!EW,!Harris!DM.!Mechanisms!for!adjusting!interaural!time!differences!to!achieve! binaural!coincidence!detection.!Journal!of!Neuroscience
Berkeley - MCB - 167
70 The Journal of Neuroscience, January 6, 2010 30(1):70 80Behavioral/Systems/CognitiveMechanisms for Adjusting Interaural Time Differences to Achieve Binaural Coincidence DetectionArmin H. Seidl, Edwin W Rubel, and David M. HarrisVirginia Merrill Blo
Berkeley - MCB - 167
MCB!167! Discussion!assignment!for!Friday,!March!5! Mechanisms!for!learning!of!sound!location!in!barn!owls!! Reading!:! Linkenhoker!BA,!von!der!Ohe!CG,!Knudsen!EI.!Anatomical!traces!of!juvenile!learning!in!the!auditory! system!of!adult!barn!owls.!Nature!
Berkeley - MCB - 167
ARTICLES 2005 Nature Publishing Group http:/www.nature.com/natureneuroscienceAnatomical traces of juvenile learning in the auditory system of adult barn owlsBrie Ann Linkenhoker1,3, Christina G von der Ohe2 & Eric I Knudsen1Early experience plays a po
Berkeley - MCB - 167
Discussion 6: Central Pattern Generators MCB 167 Spring 2010. For Discussion Section Mar 12. Reading Wyart C et al, "Optogenetic dissection of a behavioural module in the vertebrate spinal cord." Nature 461:407-10. 2009. Overview This paper uses optogenet
Berkeley - MCB - 167
Vol 461 | 17 September 2009 | doi:10.1038/nature08323L ETTERSOptogenetic dissection of a behavioural module in the vertebrate spinal cordClaire Wyart1*, Filippo Del Bene2*, Erica Warp1, Ethan K. Scott2cfw_, Dirk Trauner3, Herwig Baier2 & Ehud Y. Isacof
Berkeley - MCB - 167
Discussion 7: Treatment of Parkinson's with L-DOPA MCB 167 Spring 2010. For Discussion Section Mar 19. Reading Yahr et al (1969) Treatment of Parkinsonism With Levodopa. Arch Neurol 29: 343-354. Overview This classic paper examines the effect of L-DOPA ad
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Treatment of Parkinsonism WithSome turn this sickness yet might take, Even yet. But he: "What drug can make A wither'd palsy cease to shake?" "The Two Voices" \p=m-\Tennyson1833LevodopaMelvin D. Yahr, MD; Roger C. Duvoisin, MD; Myrna J. Schear, MD; Rob
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Circadian Rhythms (and Sleep)1.Innate, autonomous Rhythm persists in a uniform environment with a 24hr period Rhythm maintained over a wide range of temperatures Rhythm can be re-set by sensory exposure 12.Physiological rhythms controlled by circadian
Berkeley - MCB - 167
Central Pattern Generators1Rhythmic motor patterns: complex, stereotyped, repetitiveFirst systematic, scientific study of motion 1872 Gov. Leland Stanford (CA) made a bet that all four horse hooves leave the ground at the same time during gallop Hired
Berkeley - MCB - 167
Voluntary movementsPrimary motor cortex (controlling movements) Supplemental motor cortex supplementary motor area (planning movement, internal) premotor cortex (guidance of movement, external) posterior parietal cortex (integrates sensory and motor)1P
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Genetics of motor diseases1. 2. 3. 4.Multiple sclerosis (MS) Amyotrophic lateral sclerosis (ALS) Huntingtons disease Parkinsons disease1Multiple Sclerosis: many scarsPopulation frequency: 1/1000 - 1/10,000 Age of onset: 20-40 yrs old Phenotype: loss
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3/23/2010Neurobiologyofcommunication Communication isthetransferofinformationfromoneorganismtoanother. Canbeacoustic(speech,calls),olfactory(pheromones),visual(facialexpressions, othervisualcues),tactile,orevenelectrosensory(inelectricfish). Communicatio
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