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exam 3 quizzes

Course Number: BIOL 1404, Fall 2010

College/University: Texas Tech

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CHAPTER 11: MEMBRANE STRUCTURE AND FUNCTION 1) In considering the movement of small molecules across a lipid bilayer, the permeability coefficient can be correlated with C. the solubility of the molecule in a nonpolar solvent. - The greater the solubility in a nonpolar medium, the more rapidly a molecule crosses a lipid bilayer. 2) The most common way in which integral membrane proteins span the membrane is in B....

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11: CHAPTER MEMBRANE STRUCTURE AND FUNCTION 1) In considering the movement of small molecules across a lipid bilayer, the permeability coefficient can be correlated with C. the solubility of the molecule in a nonpolar solvent. - The greater the solubility in a nonpolar medium, the more rapidly a molecule crosses a lipid bilayer. 2) The most common way in which integral membrane proteins span the membrane is in B. -helical segments. - Alpha helices offer an energetically favorable way for a protein to span the membrane in terms of having hydrophobic R-groups on the outside and the polar peptide bonds hydrogen bonded together on the inside.) 3) Which membrane component exhibits the fastest movement within the bimolecular sheets? A. Phospholipids undergoing lateral diffusion. - Within membranes, phospholipids can travel along one side of the bimolecular sheet very rapidly, in a process called lateral diffusion 4) Which of the following factors makes the membrane environment more ordered and less fluid? B. the presence of cholesterol - Cholesterol makes eukaryotic membranes less fluid. The bulky ring structure of cholesterol interferes with the typical motions of the fatty acid tails of phospholipids. In addition, cholesterol appears to have some specific interactions with certain components (such as sphingolipids), forming less mobile raft-like complexes. 5) In passive transport (also known as facilitated diffusion), the energy driving the movement of materials across a membrane is provided by C. the concentration gradient across the membrane - Materials move from regions of high concentration to low concentration with the driving force being the concentration gradient itself 6) The Na+-K+ pump is an active transport system that A. pumps sodium ions out of the cell and potassium ions into the cell - Because the movement of each ion is against its concentration gradient, energy is needed to move them in the direction indicated 7) Which of the following is NOT true regarding P-type ATPases? B. Because of their common mechanism, they lack specificity and a given P-type ATPase can often transport different ions or molecules This statement is not true. Although they share a common mechanism, each different P-type ATPase has a high specificity for the particular molecule transported 8) Secondary transporters (or cotransporters) can be divided into symporters and antiporters. What is the basis for this distinction? A. Symporters use the energy from one molecule moving down its concentration gradient to - drive the movement of a second molecule in the same direction against its concentration gradient while antiporters drive the movement in the opposite direction - This correctly defines the difference between symport and antiport systems 9) Which of the following is NOT true of ion channels? B. Ion channels are continually open while pumps or secondary transporters depend on an energy source to open - This statement is not true. Channels can exist in both open and closed states 10) The three-dimensional structure of the potassium channel provided a rationale for the selectivity of potassium ions and the rejection of sodium ions. Which of the following is the basis for the ability of the potassium channel to discriminate between these two ions? C. A restriction in the channel allows for potassium ions to be resolvated by protein ligands but is too large to effectively resolvate sodium ions - The size of the restriction is such that potassium ions can be bound effectively but the smaller sodium ions cannot CHAPTER 14: METABOLISM (BASIC CONCEPTS AND DESIGN) 1) Metabolic reactions and processes that break down material and transform fuels into cellular energy are referred to as ________________ and those performing energy requiring biosynthetic processes as __________________. C. catabolic, anabolic - Catabolic processes are involved in degradation processes and anabolic reactions in biosynthesis 2) In a metabolic pathway, A. a reaction with a positive G can occur if coupled to a reaction with a greater negative G - As long as the overall G is negative, a reaction with unfavorable energetics can be driven if coupled to one with favorable energetics 3) Which of the following does NOT contribute to the high phosphoryl transfer potential of ATP? B. the adenine ring structure - This is not true. Other nucleotide triphosphates such as GTP have similar phosphoryl transfer potentials 4) The hydrolysis of ATP drives metabolism by A. shifting the equilibrium of the reaction - The equilbrium constant of an unfavorable reaction can be shifted by coupling that reaction to the hydrolysis of ATP 5) Which of the following vitamins participates as an activated carrier? B. Nicotinic acid - Nicotinic acid is also known as niacin or vitamin B3. In its activated form called nicotinamide adenine dinucleotide, (NADH and NAD+) it serves as a carrier of electrons in oxidation-reduction reactions 6) What material in vertebrate muscle serves as a reservoir for high-energy phosphate groups? C. creatine phosphate - The energetics favor transfer of phosphate to creatine phosphate when ATP is present in large amounts and the reverse when ATP is needed 7) During catabolic processes, the oxidation of energy-rich molecules often results in the reduction of NAD to NADH. What comparable molecule is the most commonly used reductant for reductive steps in anabolic processes? C. NADPH - NADPH is a reductant that can be oxidized to NADP+ 8) Acyl groups generated during metabolism of carbohydrates and fatty acids are activated by attachment to D. coenzyme A - Acetyl-CoA is generated during the degradation of both carbohydrates and fatty acids 9) Which of the following is NOT true regarding energy charge? A. Energy charge = [ATP]/ [ADP] + [Pi] - The correction equation for energy charge = [ATP] + [ADP] / [ATP] + [ADP] + [AMP]. The equation shown is called the phosphorylation potential = [ATP]/ [ADP] + [Pi] 10) Controlling the catalytic activity of enzymes important is in regulation of metabolic pathways. Two ways in which this may be accomplished are A. allosteric regulation and reversible covalent modification - Allosteric regulation and reversible covalent modification, phosphorylation of proteins for example, provide important regulatory mechanisms for metabolic processes CHAPTER 15: GLYCOLYSIS 1) A suspension of yeast cells is being grown under anaerobic conditions such that glucose is degraded to ethanol and carbon dioxide. If one wishes to follow this process by monitoring the release of 14CO2, at which positions in the glucose molecule would the 14C label need to be incorporated? B. carbons 3 and 4 - Both of these carbons will end up as the carboxyl group of pyruvate during glycolysis. This is the source of the released carbon dioxide 2) If an inhibitor of alcohol dehydrogenase is added to the above suspension, the cells rapidly die because C. the NAD+ needed to keep glycolysis going is not being recycled so the entire process is shut down and no ATP is produced - The inability to regenerate NAD+ for the glyceraldehyde-3-phosphate dehydrogenase reaction will bring glycolysis to a halt 3) A yeast mutant has a defective triose phosphate isomerase that is totally inactive. Which of the following would you expect if this yeast mutant were grown under anaerobic conditions? D. The ATP produced/glucose degraded ratio would be zero - In Stage I, 2 ATP are consumed. Due to the defective enzyme, only 2 ATP are produced in the remainder of the pathway, therefore net ATP production is zero. Normally, 4 ATP are produced in the last stage of glycolysis, for a net of 2 ATP/glucose 4) The mechanism of glyceraldehyde-3-phosphate dehydrogenase does NOT involve A. phosphorylation of the substrate using ATP - Phosphorylation of the substrate occurs but not at the expense of ATP 5) Although glucose is the major sugar degraded by the glycolytic pathway, other sugars such as galactose and fructose are also often present. These sugars B. are metabolized by the glycolytic pathway by conversion to intermediates in the pathway - Both galactose and fructose enter the glycolytic pathway by conversion to intermediates in the pathway 6) The three major regulatory enzymes in the glycolytic pathway are A. hexokinase, phosphofructokinase, and pyruvate kinase - These three enzymes serve key regulatory roles at these three most irreversible steps in glycolysis 7) Which enzyme in glycolysis involves the cleavage of the original 6 carbon sugar backbone into two products with 3-carbon backbones? B. Aldolase - Fructose 1,6-bisphosphate is reversibly cleaved into dihydroxyacetone phosphate and glyceraldehydes 3-phopshate, each with a 3-carbon backbone 8) Which of the following are NOT products in the net reaction of 1 glucose molecule via the glycolytic pathway? C. 2 NAD+ - This is not true. The products of glycolysis include 2 NADH, the reduced form of nicotinamide adenine dinucleotide. Two NAD+ are needed as reactants when 1 glucose undergoes glycolysis 9) The condition called galactosemia results from the deficiency in which enzyme? C. Galactose-1-phosphate uridyl transferase - Galactose-1-phosphate uridyl transferase converts galactose 1-phosphate and UDP- glucose into UDP-galactose and glucose-1-phosphate. Elevated concentrations of galactose in the blood can be detected in individuals with a genetic deficiency in this enzyme 10) Which of the following molecules is involved in a process called feedforward stimulation? A. Fructose-2,6-bisphosphate - F-2,6-BP is a regulatory molecule that activates phosphofructokinase (PFK), by increasing the ability of PFK to bind its substrate, fructose 6-phosphate CHAPTER 17: PREPARATION FOR THE CYCLE 1) Which of the following statements describes where the citric acid cycle occurs? B. All enzymatic steps of the citric acid cycle occur within the mitochondria - Pyruvate, generated by glycolysis, is transported into the mitochondrial matrix, where it is converted into acetyl CoA by pyruvate dehydrogenase complex. All of the other enzymatic steps of the citric acid cycle occur within the mitochondria constituting the acetyl group are 2) In the reaction catalyzed by the pyruvate dehydrogenase complex, the two carbons C. transferred to the lipoamide by an earlier intermediate in the process - The two carbons are first attached to thiamine pyrophosphate and then transferred to the lipoamide 3) Which of the following is NOT true regarding the vitamin B1 cofactor for the pyruvate dehydrogenase complex? D. Another name for this essential B vitamin is lipoic acid - This is not a true statement. Lipoic acid is not a B vitamin, but it is essential for the series of transformations of hydroxyethyl TPP into acetyl CoA. Lipoic acid is involved reversible oxidation-reduction of two sulfhydryl groups to form a cyclic disulfide 4) Although the pyruvate dehydrogenase complex is subject to allosteric control, especially inhibition by reaction products, the main regulatory process controlling the enzymes' activity in eukaryotes is A. phosphorylation by ATP that turns off the complex and dephosphorylation that turns it on - High levels of ATP are indicative of a situation where no further ATP is needed and thus the enzyme is turned off by phosphorylation 5) Which of the following is true regarding dietary thiamine? A. Lack of dietary thiamine can result in condition called beriberi - Beriberi is a physiological disorder first observed in the Far East in the mid 1600s, among populations where processing rice resulted in a decrease in its thiamine content. Beriberi results in both neurological and cardiovascular disorders 6) How does arsenite (AsO3H2) inhibit the pyruvate dehydrogenase complex? B. Arsenite reacts with the two free sulfhydryl groups on the dihydrolipoamide of the E3 enzyme in this complex - Arsenite reacts with the two free SH groups and prevents them from undergoing their usual oxidation-reduction steps that are essential to restore the enzyme complex

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