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Simon Fraser - KIN - 142
1KINESIOLOGY 142 LABORATORY REVIEW QUESTIONS - PART 2 Laboratory #7 Cardio-Respiratory Anatomy 1. Be able to identify the anatomical structures listed on pages 16-1 and 16-2 in the Kinesiology 142 Laboratory Manual. 2. Define atria and ventricles. Which
UC Davis - CHE - 118A
ToupadakisCHEM 118AKEYExam 2SPR20101 of 5Last Name _ First Name _thelifecurve.comLab Sec. # _; TA: _; Lab day/time: _ Last 4 digits of student ID number: _Dr.Toupadakis Spring 2010"Not everything that counts can beCHEMISTRY 118A EXAM 2Instruct
UC Davis - CHE - 118A
Quiz 5: Tuesday Name: Section: Date: 1. Circle the chiral compounds (3pts)OH Me Br H H Br OH Br H Br HHO2C HCO2H HHO2C HH CO2H2. Assign R or S configuration to all stereocenters in the following molecules (4pts)O H HN H H OMe OMeHOHO3. Circle t
UC Davis - CHE - 118A
TuesdayWeek 6: Tuesday Name: Date:1. Where might the following compounds have IR absorptions? (Identify three for each of the compounds) (4 pts) CH2OH HC IR absorption 1. functional group 1.C OO functional groupIR absorption2.2.3.3.2. For the fo
UC Davis - BIS 101 - BIS 101
BIS 101-001 (Engebrecht) Spring 2010 Form A DO NOT OPEN UNTIL INSTRUCTEDFinalFill out your name, student identification number and form letter on the blue Scantron Form with a #2 pencil. READ THE QUESTIONS CAREFULLY BEFORE YOU ANSWER. Fill in your answe
UC Davis - BIS 101 - BIS 101
BIS 101-003 (Engebrecht) Fall 2008Name: _ Last, First ID #:_BIS 101-003 - MIDTERM IIAThis exam has a total of 100 points. READ THE QUESTIONS CAREFULLY BEFORE YOU ANSWER. SHOW ALL WORK TO OBTAIN FULL CREDIT. Please sign your name on the score sheet. Thi
UC Davis - BIS 101 - BIS 101
BIS101-001/EngebrechtSpring 2010Review Quiz please enter answers on the Course site listed under Tests & Quizzes (https:/smartsite.ucdavis.edu/xsl-portal/site/cc31da6c-ce07-42ac-b87eb3fd0f69ec3a/page/16096a7c-90c2-49d0-970b-80316e7e533d). You will only
UC Davis - BIS 101 - BIS 101
Homework assignment #1 Due in your session, the week of 4/4/10-4/9/10 3 problems 1. (16pts) In mice, a recessive mutation in gene T results in tail-less animals and a second unlinked recessive mutation in gene F results in fat animals. Indicate the genoty
UC Davis - BIS 101 - BIS 101
Homework02key2. You have obtained two true-breeding strains of mice, each homozygous for an independently discovered recessive mutation that prevents the formation of hair on the body. The discoverer of one of the mutant strains calls his mutation naked,
UC Davis - BIS 101 - BIS 101
BIS101-001/Engebrecht Suggested problems and homework for week #3 Problems suggested for the whole class these are not to be turned in, but are for practice/study aid. The following problems can be done after the linkage lectures: Ch. 4: in both 8th and 9
UC Davis - BIS 101 - BIS 101
From Homework03: You are trying to determine the position on the E. coli chromosome of a new mutation that you isolated, called slo- (slow growing). You do generalized P1 transduction experiments using donor and recipient strains as shown below. You obser
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht Homework05 1. Mutants of Neurospora were selected on the basis of their requirement for compound B. The following data were obtained from growth tests in which the intermediate metabolites A, C, D, E, and F are supplied singly (all of th
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht Homework06 1. The following DNA is part of a gene that codes for a polypeptide of at least seven amino acids: 3' c a a t t g a t t a g t c a g t c a a t t g a t 5' 5' g t t a a c t a a t c a g t c a g t t a a c t a 3' Please see codon ta
UC Davis - BIS 101 - BIS 101
6. (12 pts) Predict the molecular consequences of the following mutations on an essential mouse ribosomal protein gene and the probable phenotype of the homozygous mouse for that particular mutation. Please give the reasoning for you answers. a. A +1 fram
UC Davis - BIS 101 - BIS 101
Homework07bkey2. Sequence analysis of an unc gene in C. elegans reveals that it encodes for a myosin, a component of muscles. As a good geneticist, you are interested in understanding the nature of the different alleles of the unc gene. You PCR unc from
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht Homework08 2. You have isolated a large number of C. elegans mutants that are uncoordinated (unc). Seven of these mutants (mutants 1-7) are recessive and you perform complementation tests with the following results: 1 2 3 4 5 6 7 1 2 + 3
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht Homework09 In 8th edition: Chapter 11: 17, 21, 22, 29, 31, 34, 35; Chapter 12: 32, 35, 36 In 9th edition: Chapter 20: 16, 18, 21, 22 1. How would you go about cloning the yeast HIS4 gene? Assume that you have a yeast his4 mutant and a ge
UC Davis - BIS 101 - BIS 101
BIS101-001/Engebrecht Spring 2010 Outline01Lectures1-2; Introduction and Mendel I. Class Organization Discussion of syllabus and all organizational matters concerning the course. Online Review Quiz due April 9 by 11:55pm. Reading for introduction. Chapter
UC Davis - BIS 101 - BIS 101
BIS101-001/Engebrecht Spring 2010 Outline02Lectures3-4: Sex-linkage, human genetics, mitosis, meiosis Reading Chapter 2, 42-58 in 8th edition or Chapter 2, 61-75 in 9th edition. I. Sex-linked inheritance a. Sex determination (chromosomal control). Homogam
UC Davis - BIS 101 - BIS 101
BIS101-001/Engebrecht Spring 2010 Outline03Lectures5-6: Allele and Gene Interactions Organisms and genes are complex. Different alleles of a single gene can result in different phenotypes. In addition, multiple genes interact resulting in unexpected pheno
UC Davis - BIS 101 - BIS 101
BIS101-001/Engebrecht Spring 2010 Outline04Lectures7-8: Genetic Linkage and Mapping Thus far, we have examined how genes segregate and influence each other. We will now extend our definition of the gene to include residing on a fixed location on a chromos
UC Davis - BIS 101 - BIS 101
BIS101-001/Engebrecht Spring 2010 Outline05Lectures9-10: Bacterial and Viral Genetics The genetics of bacteria and viruses have been instrumental for elucidating basic cell and molecular processes. They have also been the workhorses for recombinant DNA te
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht Spring10 Outline06Lectures11-12 DNA Structure and Organization in Chromosomes Reading in 8th edition Chapter 7, 227-236, in 9th edition, Chapter 7, 265-275 I. DNA as hereditary material We will only briefly cover this in class but I have
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht S10 Outline07Lecture13DNA ReplicationReading in 8th edition Chapter 7/236-249; 9th edition Chapter7/275-291. I. Semiconservative mode of replication Meselson/Stahl experiment II. Basic Mechanism of Replication DNA is synthesized by the
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht S10 Outline08Lecture14 Genetics of Gene Function Reading in 8th edition Chapter 6, 186-192 and Chapter 5, 166-170; 9th edition Chapter6, 230-235I.One gene-one enzyme hypothesis: Beadle and Tatums experiments in Neurospora a. Complemen
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht S10 Outline09Lectures15-16 Prokaryotic Transcription, Translation and the Genetic Code Reading in 8th edition Chapter 8, 255-270; 9th edition Chapter 8, 295-304 I. Transcription = making RNA from DNA (Handout14Transcription Directionalit
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht S10 Outline10Lectures17-19 Regulation of Gene Expression in Prokaryotes and Eukaryotes Reading in 8th edition Chapter 10, 301-316; 9th edition Chapter 10, 351-379 I. The lac operon of E. coli An operon = a genetic unit of coordinately ex
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht Spring2010 Outline11; Lectures 20-21 Gene Mutations, DNA Repair, Recombination and Cancer Mutations are heritable changes in the DNA sequence. Mutations can be caused by many different means (spontaneous, induced, transposition). Mutagen
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht Spring2010 Outline12Lecture22 Chromosome Mutations-Structure and Number Changes in chromosome structure by mutations, whether spontaneous or induced, are of great importance to the field of genetics as well as medicine. Chromosomal mutat
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht Spring2010 Outline13Lecture23 TransposonsReading in 8th edition Chapter 13, 423-446; in 9th edition Chapter 14, 487-510.I. II.Transposons= jumping genes. Discovered by McClintock in 1944 as mutations causing phenotypes that could not
UC Davis - BIS 101 - BIS 101
BIS101/Engebrecht Spring2010 Outline14Lecture 24-27 Recombinant DNA Technology and Genomics Recombinant DNA technology has transformed biology in the last 25 years. All of the techniques are the application of enzymes, processes, and paradigms we have enc
UC Davis - BIS - 101
BIS101-001/Engebrecht Spring 2010 Handout01BasicGeneticTerms This is a review of basic genetic terminology that we will use throughout this course. Read Chapter 1 assignment thoroughly if this doesnt seem familiar to you; your text also has an excellent g
UC Davis - BIS - 101
BIS101-001/Engebrecht Spring 2010 Handout02Mendel The Foundation of Genetics by MendelMendels experiments culminated in five conclusions to explain heredity. These conclusions are still valid today, but they were extended by the work on genetic linkage a
UC Davis - BIS - 101
BIS101-001/Engebrecht Spring 2010 Handout03Human Pedigrees Autosomal Recessive 1. Affected children often from unaffected parents. 2. On average 1/4 of children are affected in mating of 2 heterozygotes. 3. Equal numbers of affected males and females. 4.
UC Davis - BIS - 101
BIS101-001/Engebrecht Spring 2010 Handout04Mitosis/MeiosisDivision InterphaseMitosis Ploidy = 2N DNA content = 2C Chromosomes decondensed DNA synthesis in S Ploidy = 2N DNA content = 4C Chromosomes condense, sister chromatids visible, cohesion holds sis
UC Davis - BIS - 101
BIS101-001/Engebrecht Spring 2010 Handout05GeneticLinkage
UC Davis - BIS - 101
Handout6TwoPointCross
UC Davis - BIS - 101
Handout7ThreePointCross
UC Davis - BIS - 101
BIS101-001/Engebrecht Spring 2010 Handout08ChiSquare A cross is made between AABB and aabb individuals. From the AaBb F1 individuals, the following meiotic products are obtained. Do the data suggest that the A and B loci are linked? AB 280 ab 270 Ab 220 a
UC Davis - BIS - 101
Handout9ConjugationMapping
UC Davis - BIS - 101
Handout10TransductionMapping
UC Davis - BIS - 101
BIS101/Engebrecht: Experimental Proof that DNA was the Hereditary Material Handout11The experiments by Griffith (1928), Avery, MacLeod, and McCarty (1944), and Hershey and Chase (1952) established that DNA is the genetic material. Further work by Gierer
UC Davis - BIS - 101
BIS101/Engebrecht S10 Handout16 POSSIBLE MODELS OF TRANCRIPTIONAL ACTIVATION Model 1: + activator - activator TRANSCRIPTIONAL ACTIVATOR active mRNA_> X inactive _/_ regulatory region open reading frameModel 2: - repressor + repressorTRANSCRIPTIONAL REPR
UC Davis - BIS - 101
BIS101/Engebrecht S10 Handout18Transcription and other processes at the RNA levelPROKARYOTES monocistronic and polycistronic -10 and -35 sites for RNA polymerase and sigma factor binding 1 RNA polymerase different types of sigma factors promoter and ope
UC Davis - BIS - 101
BIS101 Lecture topic 12 Handout 4PROKARYOTEStranscription starttranslation startstranslation stopst s5'binding of RNA Pol + sigma factor -35 -10AUG protein 1STOPAUGSTOP protein 2AUG STOP protein 3binding sites for transcriptional regulatorsS
UC Davis - BIS - 101
BIS101/Engebrecht Handout21Mutations Transitions = base changes from one purine (A and G) to another purine, or one pyrimidine (C and T) to another pyrimidine Example: AG, TC Transversion = base changes from a purine to a pyrimidine or a pyrimidine to a p
UC Davis - BIS - 101
BIS101/Engebrecht Handout22Rev/SupWild-type gene: encodes for luciferase (an enzyme that produces light), therefore cells make light _ mutagenesis Mutant gene (single transition or transversion): no light _x_ 1. Reversion: light _ 2. Intragenic suppresso