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Biology 105C Fall 2007: Study Guide for Lab Exam 2 This is only a GUIDE. As such, it is only a starting place for studying. You are responsible for all material covered in lab. Use the materials in the lab book along with the materials and notes as material to study. Remember, the more active your studying, the more effective. Passive studying like reading and rereading over and over won't help you to really KNOW the material. Lab 15 Early developmental stages 1. List the stages of development in order from earliest to latest. 2. Briefly describe the appearance of the embryo in each developmental stage. 3. List the tissue layers found in the developing embryo. 4. Define: archenteron, zygote, notochord, neural plate, coelem. 5. Describe the role of the notochord in most vertebrates. Genetic Technology: DNA Fingerprinting Lab 1. Briefly describe how a DNA fingerprint is created and what it can be used for. 2. Describe what electrophoresis is and how it is used to generate a DNA fingerprint. 3. Identify which part of human DNA is used to make a DNA fingerprint. 4. Given a DNA fingerprint, match a known sample to an unknown sample. 5. Briefly describe how you extracted DNA from your cheek cells. Neonatal Screening Lab Describe an enzymatic pathway. Given the following conditions, describe the reason for each condition and treatment for each condition: PKU, Alkaptonuria, Galactosemia, Tyrosemia & Muccopolysaccharidosis. Describe what the positive test looks like for each of the disorders listed above. Immunology Lab 1. Define: agglutination, antigen, antibody, antiserum. 2. List the alleles that give the ABO blood typing group and all of the different blood types. 3. Given a specific phenotype, list all possible genotypes for blood type. 4. Identify which blood type is the universal donor and which is the universal recipient. 5. Identify the most and least common blood types. 6. Describe the type of inheritance shown by the ABO blood group. 7. Describe how blood typing is done and, given the results of a typing test, identify the blood type of an individual. Lab 11 Population Genetics 1. Describe what the Hardy-Weinberg equation tells us about a population (what is its purpose?). 2. List the assumptions of the Hardy-Weinberg equation (in regards to mutation, natural selection, random mating and migration). 3. Identify what each of the following parts of Hardy-Weinberg equation tells us about a population: p, q, p2, q2 and 2pq. 4. Describe what happens to allele frequencies over time in populations that meet the assumptions of the Hardy-Weinberg law. 5. Given the Hardy-Weinberg equation (p2 + 2pq + q2 = 1 and p + q =1), determine the following values: p, q, p2, q2 and 2pq. 6. Describe why recessive lethal alleles in a population may still persist even though they kill individuals that are homozygous.

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