spring08 Midterm_1
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spring08 Midterm_1

Course Number: BIOL 241, Spring 2011

College/University: Purdue

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Biol 241, Spring 2008 Midterm Exam I Purdue ID: ___________________ Question 1. Please circle the correct answers (10 points total, 1 point each) 1) Assuming that a G1 somatic cell nucleus in M. pilosula female contains 8 picograms of DNA. How much DNA would be expected in a Metaphase I cell of a female? A) 16 picograms B) 32 picograms C) 8 picograms D) 4 picograms 2) With dominant epistasis, a likely ratio...

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241, Biol Spring 2008 Midterm Exam I Purdue ID: ___________________ Question 1. Please circle the correct answers (10 points total, 1 point each) 1) Assuming that a G1 somatic cell nucleus in M. pilosula female contains 8 picograms of DNA. How much DNA would be expected in a Metaphase I cell of a female? A) 16 picograms B) 32 picograms C) 8 picograms D) 4 picograms 2) With dominant epistasis, a likely ratio resulting from a dihybrid cross would be: A) 9:3:4 B) 9:7 C) 12:3:1 D) 9:3:3:1 3) Which of the following does NOT always occur with gene conversion: A) Formation of heteroduplex B) Mismatch repair C) Generation of recombinant chromosomes D) Formation of Holliday structure 4) In Drosophila, sex is determine by a balance between the number of haploid sets of autosome and the number of A) Centromeres B) Telomeres C) X chromosomes D) Y chromosomes 5) When an organism gains or loses one or more chromosomes but not a complete haploid set, the condition is known as A) Polyploidy B) Euploidy C) Aneuploidy D) Trisomy 1 Biol 241, Spring 2008 Midterm Exam I Purdue ID: ___________________ 6) Human patterned baldness is more prominent in males because it is a A) X-linked trait B) Y-linked trait C) Sex-influenced autosomal trait D) Conditional trait 7) The F factor that mediates the transferring of genetic materials during conjugation is a A) Bacteriophage B) Circular DNA C) Cell surface protein D) Single-Stranded RNA 8) The percentage of individuals that show at least some degree of expression of a mutant genotype defines A) Expressivity B) Genetic suppression C) Penetrance D) Incoomplete dominance 9) Which one of the following is Mendels First Law A) Unit factors in pairs. B) Dominance/Recessiveness C) Equal segregation D) Independent assortment 10) A loss-of-function mutation that causes a mutant phenotype when heterozygous is called A) Dominant active B) Dominant negative C) Haplo-insufficient D) Dominant suppressor 2 Biol 241, Spring 2008 Midterm Exam I Purdue ID: ___________________ Question 2. (10 points) In unicorns, two unlinked autosomal loci interact to determine the type of tail. One locus controls whether a tail is present at all; the allele for a tail (T) is dominant over the allele for tailless (t). If a unicorn has a tail, then alleles at a second locus determine whether the tail is curly or straight. Farmer Baldridge has two unicorns with curly tails. When he crosses these two unicorns, of the progeny have curly tails, have straight tails, and do not have a tail. Please give the genotype of the parents in Farmer Baldridges cross and briefly explain how he obtained the 2:1:1 phenotypic ration. Question 3. (10 points) A geneticist is working with a new bacteriophage called phage Y3 that infects E. coli. He has isolated eight mutant phages that fail to produce plaques when grown on E. coli strain K. To determine whether these mutations occur at the same functional gene, he simultaneously infects E. coli K cells with paired combinations of the mutants and looks to see whether plaques are formed. He obtains the following results. (A plus sign means that plaques were formed on E. coli K; a minus sign means that no plaques were formed on E coli K.) Mutant 1 2 3 4 5 6 7 8 1 + + + + 2 + + + + 3 4 5 6 7 8 + + + + + + + + + - + - + a. To how many functional genes (cistrons) do these mutations belong? (4 points) b. Which mutations belong to the same functional gene? (6 points) 3 Biol 241, Spring 2008 Midterm Exam I Purdue ID: ___________________ Question 4. (10 points) A Neurospora cross was made between one strain that carried the mating-type allele A and the mutant allele arg-1 and another strain that carried the matingtype allele a and the wild-type allele for arg-1 (+). Four hundred linear octads were isolated, and they fell into the seven classes given below. (For simplicity, they are shown as tetrads.) 1 A arg A arg a+ a+ ------127 2 A+ A+ a arg a arg ------125 3 A arg A+ a arg a+ ------100 4 A arg a arg A+ a+ ------36 5 A arg a+ A arg a+ ------2 6 A+ a arg A+ a arg ------4 7 A+ a arg A arg a+ ------6 a. Deduce the linkage arrangement of the mating-type locus and the arg-1 locus. Include the centromere or centromeres on any map that you draw. Label all intervals in map units. (6 points) b. Diagram the meiotic divisions (draw the chromosomes) that led to class 6. Please label clearly. (4 points) 4 Biol 241, Spring 2008 Midterm Exam I Purdue ID: ___________________ Question 5. (10 points) In a generalized transduction experiment, phages are collected from an E. coli donor strain of genotype cys+ leu+ thr+ and used to transduce a recipient of genotype cys- leu- thr-. Initially, the treated recipient population is plated on a minimal medium supplemented with leucine and threonine. Many colonies are obtained. a. What are the possible genotypes of these colonies? (3 points) b. These colonies are replica-plated then onto three different media: (1) minimal plus threonine only, (2) minimal plus leucine only, and (3) minimal. It is observed that 56 percent of the original colonies grow on medium1, 5 percent grow on medium 2, and no colonies grow on medium 3. What are the actual genotypes of the colonies on media 1, 2, and 3 (although no colony grows on media 3, please state the genotype(s) of colonies that could grow on media 3)? (3 points) c. Draw a map showing the order of the three genes and which of the two other genes is closer to the middle gene. (4 points) 5 Biol 241, Spring 2008 Midterm Exam I Purdue ID: ___________________ Question 6. (10 points) Two male mice, which we will call male A and male B, are both phenotypically normal. Male A was from a litter that contained half phenotypically normal mice and half dwarf mice. The mother of male A was known to be homozygous for the normal Igf2 allele. Male B was from a litter of eight mice that were all phenotypically normal. The parents of male B were a phenotypically normal male and a dwarf female. Male A and male B were put into a cage with two female mice that we will call female A and female B. Female A is dwarf and female B is phenotypically normal. The parents of these two females were unknown, although it was known that they were from the same litter. The mice were allowed to mate with each other, and the following data were obtained: Female A gave birth to three dwarf babies and four normal babies. Female B gave birth to four normal babies and two dwarf babies. Which male(s) mated with female A and female B? (2 points) Please briefly explain. (8 points) 6 Biol 241, Spring 2008 Midterm Exam I Purdue ID: ___________________ Question 7. (10 points) As discussed in class, there are the fast and slow alleles of glucose-6-phosphate dehydrogenase (G-6-PD), which is an X-linked gene. Why does a clone of mouse cells produce only one type of G-6-PD enzyme? (3 points) What would you expect to happen (that is, produces only one type or both types) if a clone was derived from an early embryonic cell? Please briefly explain your answer. (4 points) If fast and slow alleles of G-6-PD exist in Drosophila and it is also X-linked, what would be the expected results of gel electrophoresis for a heterozygous female (that is, produces only one type or both types)? Please briefly explain your answer. (3 points) 7 Biol 241, Spring 2008 Midterm Exam I Purdue ID: ___________________ Question 8 (10 points) Waxy endosperm (wx), shrunken endosperm (sh), and yellow seedling (v) are encoded by three recessive genes in corn that are linked on chromosome 5. A corn plant homozygous for all three recessive alleles is crossed with a plant homozygous for all the dominant alleles. The resulting F1 are then crossed with a plant homozygous for the recessive alleles in a three-point testcross. The progeny of the testcross are: a. Determine the order of these genes on the chromosome (3 points). b. Calculate the map distances between the genes (4 points). c. Determine the coefficient of coincidence and the interference among these genes (3 points). 8 Biol 241, Spring 2008 Midterm Exam I Purdue ID: ___________________ Question 9. (10 points) A boy with Klinefelter syndrome (47, XXY) is born to a mother who is phenotypically normal and a father who has the X-lined skin condition called anhidrotic ectodermal dysplasia. The mother skin is completely normal with no signs of the skin abnormality. In contrast, her son has patches of normal skin and patches of abnormal skin. a. Which parent contributed the abnormal gamete? (2 points) b. Using the appropriate genetic terminology, describe the meiotic mistake that occurred. Be sure to indicate which division the mistake occurred in. (4 points) c. Using the appropriate genetic terminology, explain the sons skin phenotype. (4 points) 9 Biol 241, Spring 2008 Midterm Exam I Purdue ID: ___________________ Question 10. (10 points) A researcher has several different strains (1, 2, and 3) of T4 phage with single mutations in the same gene. In these strains, the mutations render the phage temperature sensitive. This means that temperature-sensitive phages can propagate when the bacterium (E. coli) is grown at 32C but cannot propagate themselves when E. coli is grown at 37C. E.coli cells were simultaneously infected with strains 1 and 2 at 32C. After the cells had lysed, samples of phage lysate were collected. One sample was grown on E. coli at 32C and a second sample at 37C. The results are summarized below: Temperature plated 32C 37C Dilution 10-5 10-1 plaques 2 5 a. What is the recombination frequency between these two mutations? (5 points) b. Strain 3 was also simultaneously infected with either strain 1 or 2. However, no plaques were recovered from the 37C plates. Based on we discussed in the class, what is the nature of mutation in strain 3? (5 points) 10

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