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Course: BIO 415, Fall 2008School: Purdue
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HowdoyousealuptheendsofDNAs? 9/24/08ManipluatingDNAI,contd restrictionenzymetocleave DNAligasetoreseal DNAligaseligatesafree5Panda3OHendtoproduceacovalentphosphodiester bond HowcanyouoptimizecloningaDNAfragmentintoaplasmid? intermolecularinteractionveryhardtodo plasmidisintramolecular2endscanonlybeasfarapartassizeoftheplasmid moreeffiecientforplasmidstocomebacktogetherandinsertdoesntgetin....
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HowdoyousealuptheendsofDNAs? 9/24/08ManipluatingDNAI,contd restrictionenzymetocleave DNAligasetoreseal DNAligaseligatesafree5Panda3OHendtoproduceacovalentphosphodiester bond HowcanyouoptimizecloningaDNAfragmentintoaplasmid? intermolecularinteractionveryhardtodo plasmidisintramolecular2endscanonlybeasfarapartassizeoftheplasmid moreeffiecientforplasmidstocomebacktogetherandinsertdoesntgetin. 1.removephosphate(withalkalinephosphatase)andreplacewithOsoplasmiddoesnt onitself close inserthasphosphateendsandwillattachtoplasmid stillformsgapat5endofplasmidwhichcellhastorepair 2.changeconcentrationsohavemanymoreinsertsandmorelikelytoattachtoplasmid Alkalinephosphataseremoves5PfromanucleicacidendandleavesfreeOH groups 3.Directionalcloningincreasesefficiency.IfcleavewithecoR1andBamH1ratherthan both endsthesame,itwontcomebacktogethernomatterwhat
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Purdue - BIO - 415
9/22/08ManipulatingDNAI RecombinantDNATechnology cloningDNAtheisolationofaspecificDNAfragmentwhichisinsertedintoan appropriate DNAvector plasmidacircularDNAmoleculethatcanfreelyreplicate(episome)inbateria,yeast, plants oranimalcells thisisaDNAvector Cloni
Purdue - BIO - 415
9/19/08MappingtheGenome,contd Figure42Somepointmutationschangetherestrictionmap Figure43Restrictionsitesshowmendelianinheritance Why?Geneticscreening,paternitytests RFLPMap(Restrictionfragmentlengthpolymorphism) randommutationsinthegenomethatarestablyinhe
Purdue - BIO - 415
9/17/08GenerOrganization1 Figure39DHFRgeneshaveaconstantstructure exonsandintronsaresamebutvariable exonsaremoreconservedacrossspeciesandwithingenefamily codingforprotein intronsarediscardedsoultimatelydoesntmattermuch Figure311Interruptedgenespredominate
Purdue - BIO - 415
9/15/08Mutations,GenesandProteins MutationschangethesequenceofDNA pointmutationschemicalmodificationsdirectlychangesonebaseintoanother (chemical mutagens) improperDNAreplication classifications transitions PyPy(ex:CT) PuPu(ex:AG) transversions PuPy(ex:AT)
Purdue - BIO - 415
9/12/08 RestrictionEnzymes Table4.1 enzymesrecognizespecificsequences hundredsofenzymes arrow=whereitcleaves tableonlyshowsupperstrand ~95%recognizepalindromes 1enzymeuntildigests1microgramofDNA/hrat37degreesC canbuyunitsonline 1stinitialof1stwordand1st2l
Purdue - BIO - 415
9/10/08DNASecondaryStructures SupercoilingthetotalamountofDNAinanyindividualcellisexceedinglylargewhenthe lengthofthe moleculeiscomparedwithdimensionsofthecell ane.colicellhas4.7x106bpDNAwhichis~1.6mm supercoilingallowsDNAtoadoptamorecompactstructure migr
Purdue - BIO - 415
9/8/08SecondaryStructures DNAcanbedenaturedandrenatured seefigure123 Hybridizationtheabilityof2nucleicacidnoleculestohybridizeconstitutesaprecisetestfor their sequencecomplementarity(similarities)andthereforesuggestshowrelatedthe2 moleculesaretooneanother
Purdue - BIO - 415
9/3/08 DNAPrimaryStructure1 TheStructureofDNA 1950ErwinChargaff nucleotidecompositionbetweenspeciesdiffers ATCG [purines]=[pyrimidines] (A,G) (C,T) [A]=[T]and[G]=[C] ThisisknownastheEquivalenceRule 1953JamesWatson,FrancisCrick,MauriceWilkins,RosalindFrank
Purdue - BIO - 415
8/29/08stDNAasthegeneticmaterial2 FriedrichMieschner,MDWho1 discoveredDNA? Inwhatyear?1869gotcellsfromoldbandaids isolatednuclein 1928FrederickGriffithstransformingprinciple pneumococcuspathogenicbacterium transformation Figure13 Conclusions: Sstraini
Purdue - BIO - 415
8/27/08DNAasthegeneticmaterial1 Cellssmallestlivingunit permeablemembrane selfreplicate2identicalcells(nearperfectcopies) slightchangesoccur Prokaryotes bacteria,bluegreenalgae cellwall(polysaccharides) plasmamembrane(lipid/protein) nonucleus Eukaryotes y
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DramaticImaginationFall2009 StudyGuide:Exam#1 10/13/09 Whatarethethreemostessentialelementsfortheatre? HowdoesEricBentleydescribetheatre? WhatistheWillingSuspensionofDisbelief? Whatarethefivequalitiesoftheatre?Andhowaretheydefined? Whatistheprimerequisit
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DRAMATICIMAGINATIONFall2009LECTURENOTES Week1Tuesday WhatwasthefirstPerformance? Ritual:RainDanceworshipofgods Eachsocietydevelopsnumerousconventionswhichserveasunconsciousguidelinesof behaviorthatmaybeviewedasritualsthatdefinesocietalrelationships,andits
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ReviewofWeek5&6 Eras60Minutestocover2000yearsofhistory(33.3yearsperminute) o Greek CityDionysia=4plays:3Tragedies1SatyrPlay ThespisThespianActor Playwrights:Aeschylus,Sophocles,Euripides TheatreStructure: TheatronseeingplaceTheatreTheater Orchestradancin
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September2009Sunday Monday Aug 31 Tuesday 1 Class Intro Assignment: 3 Things & Select Shows 2 Wednesday Thursday 3 Theatre History cont. Production Overview Assignment: Ch.1 & 2 10 Production Team Intro Assignment: Ch.11 Philly Fringe 17 Show Dates Confi
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The Dramatic Imagination: The Performing Arts in SocietyCourse Number: THE 0805 - Section: 001 - CRN: 075919 Tuesday/Thursday 2:00 PM - 3:20 PM Tuttleman Learning Center 101Entrance on 13th Street, North of Montgomery (#59 Campus Map)Instructor: Teleph
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TempleUniversityTheFoxSchoolofBusinessandManagement MKTG2101:MarketingManagement Section004(T/Th8920AM)ALTERHALL31SPRING2011UPDATED2/22/11Instructor:MaryConran,AssistantProfessor,DepartmentofMarketingandSupplyChain Management Office:AlterHall519 Office
Temple - MKTG - 2101
Creatingand CapturingCustomer ValueChapter 1Marketing: Marketing:1. 2. 3. 4.RestStop:Previewingthe Concepts LearningObjectivesDefinemarketingandthemarketing process. Explaintheimportanceofunderstanding customersandidentifythefivecore marketplaceconce
Temple - MKTG - 2101
Company and Marketing Strategy: MarketingPartnering to Build Customer RelationshipsChapter 21. 2. 3. 4. 5.Rest Stop: Previewing the Concepts Concepts Learning ObjectivesExplain companywide strategic planning and its four steps. Discuss how to design
Temple - MKTG - 2101
Analyzingthe Analyzingthe Marketing EnvironmentChapter 31. 2. 3. 4. 5.RestStop:Previewingthe ConceptsDescribetheenvironmentalforcesthat affectthecompanysabilitytoserveits customers. Explainhowchangesinthedemographic andeconomicenvironmentsaffect marke
Temple - MKTG - 2101
ToGainCustomerInsightsManagingMarketing ManagingMarketing InformationChapter 41. 2. 3. 4. 5.RestStop:Previewingthe Concepts LearningObjectivesExplaintheimportanceofinformation ingaininginsightsaboutthe marketplaceandcustomers. Definethemarketinginfor
Temple - MKTG - 2101
Understanding Understanding ConsumerandBusinessBuyerBehaviorChapter 51. 2. 3. 4. 5.RestStop:Previewingthe Concepts LearningOutcomesUnderstandtheconsumermarket andthemajorfactorsthatinfluence consumerbuyerbehavior. Identifyanddiscussthestagesinthe buy
Temple - MKTG - 2101
CustomerDriven CustomerDriven MarketingStrategyCreatingValuefor TargetCustomersChapter 61. 2. 3. 4.RestStop:Previewingthe ConceptsDefinethemajorstepsindesigninga customerdrivenmarketingstrategy: marketsegmentation,targeting, differentiation,andpositi
Temple - MKTG - 2101
Products, Services, and Brands andBuilding Customer ValueChapter 7Rest Stop: Previewing the Concepts Concepts1. 2. 3. 4.Define product and the major classifications of products and services. Describe the decisions companies make regarding their indiv
Temple - MKTG - 2101
and ManagingtheProductLife CycleDevelopingNew DevelopingNew ProductsChapter 81. 2. 3. 4.RestStop:Previewingthe ConceptsExplainhowcompaniesfindanddevelop newproductideas. Listanddefinethestepsinthenew productdevelopmentprocessandthe majorconsideration
Temple - MKTG - 2101
Understandingand CapturingCustomerValuePricing PricingChapter 91. 2. 3. 4. 5. 6.RestStop:Previewingthe ConceptsIdentifythethreemajorpricingstrategiesand discusstheimportanceofunderstanding customervalueperceptions,companycosts, andcompetitorstrategie
Temple - MKTG - 2101
DeliveringCustomerValueMarketingChannels: MarketingChannels:Chapter 101. 2. 3. 4. 5.RestStop:Previewingthe ConceptsExplainwhycompaniesusemarketing channelsanddiscussthefunctionsthese channelsperform. Discusshowchannelmembersinteract andhowtheyorganiz
Temple - MKTG - 2101
PersonalSelling PersonalSelling andSalesPromotionChapter 131. 2. 3. 4.RestStop:Previewingthe ConceptsDiscusstheroleofacompanys salespeopleincreatingvaluefor customersandbuildingcustomer relationships. Identifyandexplainthesixmajor salesforcemanagement
Temple - MKTG - 2101
Direct and Online Marketing: Marketing:Building Direct Customer RelationshipsChapter 14Rest Stop: Previewing the Concepts Concepts1. 2. 3. 4. 5.Define direct marketing and discuss its benefits to customers and companies. Identify and discuss the majo
Temple - MKTG - 2101
TheGlobal TheGlobal MarketplaceChapter 151. 2. 3. 4.RestStop:Previewingthe ConceptsDiscusshowtheinternationaltrade systemandtheeconomic,political legal,andculturalenvironments affectacompanysinternational marketingdecisions. Describethreekeyapproaches
Temple - MKTG - 2101
SustainableMarketing SustainableMarketingSocialResponsibilityand EthicsChapter 161. 2. 3. 4. 5.RestStop:Previewingthe ConceptsDefinesustainablemarketingand discussitsimportance. Identifythemajorsocialcriticisms ofmarketing. Defineconsumerismand envir
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FYS 141 -First Year Seminar Program Fall 2010: Social & Political Worlds9/11: A Historical SurveyInstructor: Philippa Winkler, PhD, Department of Political Science & International Affairs, NAU Contact: Via Vista email Teaching Assistant: Alissa Oliverso
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College of Arts and Letters Department of Modern Languages Spanish Immersion Program: SPA 121, 122, 221, 222 Spring of 2011 Credits: 16 hours Instructor: Daniel R. Serpas Office: BAA 305 Phone: (928) 523-7633 E-mail: daniel.serpas@nau.edu Office Hours: M-
Purdue - BIO - 585
Lecture 25 I. What is community stability - stability = tendency of a community to return to its original state after a perturbation - perturbations: fire, volcano, grazing/predation, invasive species, extinction Types of stability - resilience = speed wi
Purdue - BIO - 585
Lecture 24, contd Aquatic Systems - light must go through water to be available for photosynthesis Rate of photosynthesis vs. depth in 3 lakes in Californialight is necessary for aquatic productivity but it is not a limiting factor - productivity peaks w
Purdue - BIO - 585
Lecture 24- communities and productivity I. key concepts and definitions Autotrophs self-feeders organisms that assimilate energy from either sunlight or inorganic other Photosynthesis 6COs+6H2OC6H12o6+6O2 primaryproduction:chemicalenergygeneratedbyautotr
Purdue - BIO - 585
Lecture 23 I. The landscape mosaic - patch a relatively homogenous community type within a landscape mosaic (ex: forest patch) - landscape topographic and environmental features of a religion - ex: the number, area, and distance between mountains, valleys
Purdue - BIO - 585
Lecture22 1.Predationsandcommunitystructure communitystructure:thepatternsofspeciesabundanceandpopulationinteractions withina community typesofspeciespresent relativeabundanceofspecies vegetationcharacteristics trophicrelationshipsamongtheinteractingpopul
Purdue - BIO - 585
Lecture 21 I. Introduction to community structure - community set of coexisting and potentially interacting species - community structure patterns of species abundance and population interactions w ithin a community - competition, predation, mutualism - a
Purdue - BIO - 585
Lecture 18 I. introduction to positive interactions - positive interaction : interaction in which neither species is harmed and benefits outweigh costs for at least 1 species - commensalism : 1 species benefits without affecting the other (+/0) - mutualis
Purdue - BIO - 585
Lecture17 I.introtopredatorpreydynamics ex:pricklypearcactustookoverAustralia introducedmothtoalmostcompletelywipeitout II.predatorresponsetoprey=howpredatornumbersrespondtochangingpreynumbers (populationresponse)=howanindividualpredatorrespondstochange
Purdue - BIO - 585
Lecture16Predation I.Trophiclevels(feedinglevels),guilds,foodwebs Foodweb: Trophiclevel: carnivoresherbivoresproducers competitionwithintrophiclevels(notconsideredinfoodwebs) Predation:interactioninwhichoneorganismconsumesallorpartofanotherorganism diff
Purdue - BIO - 585
Lecture 15 - from last time: prove that the decline of a population above its carrying capacity is faster than t he corresponding increase below K Review of terms - competition: use or defense of a resource that reduces availability of that resource for a
Purdue - BIO - 585
Guestspeaker allmodelsarewrong,butsomemodelsareuseful GeorgeBox(awellknownstatistician) Intraspecificcompetition competition=useordefenseofalimitingresourcebyoneindividualthatreducesits availabilitytootherindividuals ifcompetitorsaremembersofthesamespecie
Purdue - BIO - 585
Lecture 13 Density Dependence Calculating doubling time of a population that is exhibiting exponential growth: solution: Nt =Noert 2No =Noe 2 =ert Continuous: ln2 =lnert ln2 =rtlne ln2 =rt t =ln2/r doesnt matter what population size is, doubling time is s
Purdue - BIO - 585
Lecture12Densityindependentgrowth Introtopopulationgrowthmodels becauseinterestedinspreaddynamics,impactofhumangrowth,etc. whymathmodels? 1.generatestestablepredictions 2.allowsustomakegeneralizations 3.exammechanismsunderlyingpatternsinnature iftakepreda
Purdue - CLCS - 335
CLCS335Lecture11/07/08 November13,7:309:30BeeringHallRm.2290 SportofGladiatorialCombatLectureOdysseus resemblesfolktalemorethanresembleslegend moreainosthanepos Anticlea mothertoOdysseus daughterofAutolycus sonofHermes marriedtoLaertes fatherofOdysseus S
Purdue - CLCS - 335
CLCS335Lecture11/05/08TrojanWar Achilles'sdivinearmorwhogetsit storybeginswiththeJudgmentofParis storyendswithJudgmentofArms Aias/Ajaxvs.Odysseus Ajaxcommittedtogettingkleos Odysseusnotinterestedkleos,moreinterestedingettingthejobdone committedtonostos i
Purdue - CLCS - 335
CLCS335Lecture11/3/08TrojanWar mostheroeshavealinktotheTrojanwar exchangeisacommontheme women clothing corpses GreeksandTrojansoperatewithinherowarriorcode soldiersnotfightingtogetHelenback,fightingforkleos(glory) moretime(honor)>morekleos(glory) explain
Purdue - CLCS - 335
CLCS335Lecture10/31/08TrojanWar Greeksconsideredstorytrue considerednationalandethnichistory 1184BCE HeroicAge siteVIIAshowssignsofburning centralmythtoGreekculture mythofTrojanWarprovidesframeworkforhowGreeksdealwitheasterncultures ZeusandLedaandTyndare
Purdue - CLCS - 335
CLCS335Lecture10/29/08 Blackboardisdown: web.ics.purdue.edu/~kdickson/335f2k8sched.htmlHerakles cosmogonicbattle Typhoeusoffspring death Geryon Alcestis Cerberus splitnature xenia protects violates Perseus BronzeAgeGreece twins:AcrisiusandProetus havefis
Purdue - CLCS - 335
CLCS335Lecture10/27/08Herakles parergon(sidedeed) violatesxenia killscentaurs Augeus king stablefullofhorsesthathasn'tbeencleaned Heraklesredirectsrivertoflushitout whenAugeusrefusestopay,Herakleskillshimandhischildren StymphalianMarsh oneofthenineentran
Purdue - CLCS - 335
CLCSLecture10/24/08Herakles referredtoas"heros" Heraklesisaregionalhero asopposedtolocal firstglobalhero HeraklesAlexikakos HeraklestheDefenderAgainstEvil mostfrequentlyappealedtosemidivinity matonHeraklea"swearbyHerakles" sonofZeusAlcmena Alcmenamarried
Purdue - CLCS - 335
CLCS335Test2ReviewDifferencebetweengodsandhumans dynamisvs.elpis godsdesire,theyhavedynamis humansdesire,theyhaveelpis olboskotoshybrisate binaryopposites ApolloDionysus ApolloHermes AthenaPoseidon ArtemisAphrodite/Hera HermesHestia NeedtoKnow whotheyare
Purdue - CLCS - 335
CLCS 335 Test 2 Review 10/15/08 CLCS 335 Classical Mythology Test 2 Review Guide Updates: 1.10 kouros Test 2 (Thursday, 16 October 8-9pm) will be a combination of multiple choice and T/F questions based on both assigned readings and class lectures. You sh
Purdue - CLCS - 335
CLCS335Lecture10/6/08Hermes namemeansHerm cairn pileofrocks usedtomarkaboundary canbeawarning ithyphallic straight/erectpenis erectpenisdemonstratespowerandvirility Godthatis: constantlymoving ofinterchange/commerce alwaysinthemiddle inanthropomorphicfor
Purdue - CLCS - 335
CLCS335Lecture10/8/08Dionysus worshipconsistsofkillingandbecomingGod violentshatteringofboundaries sonofZeusandSemele(amortalwoman) HeraconvincesSemeletomakeZeusappearinhistrueform,andwhenhedoesand incineratesher ZeusripsDionysusoutofherwombandcarrieshim
Purdue - CLCS - 335
CLCS335Lecture10/3/08Athenachieffunctionistodefendthecity intelligence manipulationofforces urbanization technology guilds craftsmen useofnaturalresources practicalsolutions engineering notofwisdom(thatwasaRomanthing) business olive gaveAthensanolivetre
Purdue - CLCS - 335
CLCS335Lecture10/1/08 Artemiscentralto: o o o o o Hunting Marriage Sexuality Space(woods) Presidesovervirgins o Oncesexuallyactive,femalesbelongtoHeraandAphroditeBloodmostprominentimage Hunting Menarche Sacrificeo Apollo oCourtshipequivalenttohuntingA
BYU - COM - 310
COM 318 Visual Persuasion2-Dec-09 The images and visual cues around us affect our actions and behaviors o Print, TV, Outdoor media, Web, Personal styles, statements, Art, Graffiti, buses. o Powerpoints/Oral communication; Visual persuasion is all pervas
BYU - COM - 310
COM 318 Motivational Appeals Emotion and Persuasion30-Nov-09 Making people feel good, or bad, can be an effective persuasive strategy. Warmth appeals make people feel sentimental or nostalgic by emphasizing family, friends, and a sense of belonging o Ha
BYU - COM - 310
COM 318 Communication Accommodation Theory23-Nov-09 Expansion of Speech Accommodation theory to nonverbal communication o When people hang out for a long time they pick up on each others speech style Accents, swearing, twang Interpersonal synchronizatio