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University of Florida - PLS - 4601c
Weed Science, PLS 4601c Section 7644and Grad. Prin. Of Weed Science AGR 6932 Section 9212University of Florida - Daviehttp:/grove.ufl.edu/~turf/weedscience/Philip Busey, turf@ufl.edu954-579-3932 (cell)June 3, 2009Material from May 28 for Quiz #4 on
University of Florida - PLS - 4601c
Student Name _Weed Science, PLS 4601c Section 7644and Grad. Prin. Of Weed Science AGR 6932 Section 9212University of Florida - Daviehttp:/grove.ufl.edu/~turf/weedscience/Philip Busey, turf@ufl.edu954-579-3932 (cell)June 11, 2009Quiz 51.The weed
University of Florida - PLS - 4601c
Student Name _Weed Science, PLS 4601c Section 7644and Grad. Prin. Of Weed Science AGR 6932 Section 9212University of Florida - Daviehttp:/grove.ufl.edu/~turf/weedscience/Philip Busey, turf@ufl.edu954-579-3932 (cell)June 11, 2009Quiz 51.The weed
University of Florida - PLS - 4601c
Student Name _Weed Science, PLS 4601c Section 7644and Grad. Prin. Of Weed Science AGR 6932 Section 9212University of Florida - Daviehttp:/grove.ufl.edu/~turf/weedscience/Philip Busey, turf@ufl.edu954-579-3932 (cell)July 2, 2009Quiz 61.Which of t
University of Florida - PLS - 4601c
Student Name _Weed Science, PLS 4601c Section 7644and Grad. Prin. Of Weed Science AGR 6932 Section 9212University of Florida - Daviehttp:/grove.ufl.edu/~turf/weedscience/Philip Busey, turf@ufl.edu954-579-3932 (cell)July 2, 2009Quiz 61.Which of t
University of Florida - PLS - 4601c
Student Name _Weed Science, PLS 4601c Section 7644and Grad. Prin. Of Weed Science AGR 6932 Section 9212University of Florida - Daviehttp:/grove.ufl.edu/~turf/weedscience/Philip Busey, turf@ufl.edu954-579-3932 (cell)July 16, 2009Quiz 7Study the at
University of Florida - PLS - 4601c
Student Name _Weed Science, PLS 4601c Section 7644and Grad. Prin. Of Weed Science AGR 6932 Section 9212University of Florida - Daviehttp:/grove.ufl.edu/~turf/weedscience/Philip Busey, turf@ufl.edu954-579-3932 (cell)July 16, 2009Quiz 7Study the at
University of Florida - PLS - 4601c
Weed Science, PLS4601c, Section 7644University of Florida - Daviehttp:/grove.ufl.edu/~turf/weedscience/Philip Busey, turf@ufl.edu954-579-3932 (cell)May 14, 2009(revised June 3, 2009)SyllabusGeneralWeed Science is a 3-credit hour class consisting
University of Florida - PLS - 4601c
Herbicide Company GenealogyThe following chart is intended to show the history of the major U.S. herbicidecompanies. The information depends largely on peoples memories, which sometimescan be inexact, on records from the headquarters of major companies
University of Florida - PLS - 4601c
Weed Science, PLS 4601c Section 7644and Grad. Prin. Of Weed Science AGR 6932 Section 9212University of Florida - Daviehttp:/grove.ufl.edu/~turf/weedscience/Philip Busey, turf@ufl.edu954-579-3932 (cell)June 4, 2009Weed Flora of the UF-Davie Landscap
University of Florida - PLS - 4601c
Vegetative key to 50 common landscape weeds of South FloridaDr. Phil BuseyD R A F TPlease send me your comments11Leaves 10 times longer than wide and veins parallelIF NOT SKIP TO 242Upright stems somewhat triangular and leaves very shiny .CONTINUE
University of Florida - PLS - 4601c
Weed Science, PLS 4601c Section 7644 and Grad. Prin. Of Weed Science AGR 6932 Section 9212University of Florida Davie http:/grove.ufl.edu/~turf/weedscience/Philip Busey, turf@ufl.edu954-579-3932 (cell)July 13, 2009Common weeds set 2GenusspeciesComm
University of Florida - PLS - 4601c
Florida has twoseasons, wet and dryAdapted plants cansurvive both.RainIrrigationEvapotranspirationRainEvapotranspiration(water use)IrrigationRunoffRoot zonewaterreservePercolationRadiantenergyRelativehumidityEvapotranspirationTemperat
University of Florida - PLS - 4601c
Turfgrass HealthIdentify Weeds by Flowers and FruitsIdentifyMatchweed flowersare in small headsVirginia buttonweedfruits are shaped likeelongate buttonsTurfgrass HealthGrass WeedsGrassWeed grasses are often aggressivecreepers in turf areasTur
University of Florida - HOS - 6737c
Chapter 10Adventitious Regeneration1. INTRODUCTIONsomatic embryos arise from single cells, or groups ofcells, which become induced by the culturalconditions to become centres of active cell division(adventitious meristems), each capable of producing
University of Florida - HOS - 6737c
Additional Transformation TopicsChloroplast TransformationFor containing transgenes (plastids nottransferred through pollen?)Chloroplast transformation involveshomologous recombinationLeaf discs are bombarded with plasmidconstructs containing a sel
University of Florida - HOS - 6737c
Plant Physiology and Biochemistry 43 (2005) 161167www.elsevier.com/locate/plaphyEffect of the application of benzyladenine pulse on organogenesis,acclimatisation and endogenous phytohormone content in kiwi explantscultured under autotrophic conditions
University of Florida - HOS - 6737c
Chapter 5Plant Growth Regulators I:Introduction; Auxins, their Analogues and Inhibitors1. HORMONES, GROWTH SUBSTANCES AND GROWTH REGULATORSSome chemicals occurring naturally within planttissues (i.e. endogenously), have a regulatory, ratherthan a nu
University of Florida - HOS - 6737c
Plant Cell, Tissue and Organ Culture 76: 189191, 2004. 2004 Kluwer Academic Publishers. Printed in the Netherlands.189Research noteInuence of liquid pulse treatment with growth regulators on in vitropropagation of banana (Musa spp. AAA)P. Madhulatha
University of Florida - HOS - 6737c
Basic Laboratory Procedures Involved in Media Making:The majority of laboratory operations utilized in the in vitro propagation of plants can beeasily learned. One needs to concentrate mainly on accuracy, cleanliness, and strictadherence to details whe
University of Florida - HOS - 6737c
Basic Laboratory Procedures Involved in Media Making:The majority of laboratory operations utilized in the in vitro propagation of plants can beeasily learned. One needs to concentrate mainly on accuracy, cleanliness, and strictadherence to details whe
University of Florida - HOS - 6737c
Biolistic PDS-1000/He Particle Delivery SystemDemonstrationIntroductionBiolistic particle delivery is a method of transformation that uses helium pressure tointroduce DNA-coated microcarriers into cells. Microprojectile bombardment cantransform such
University of Florida - HOS - 6737c
The Plant Cell, Vol. 17, 27382753, October 2005, www.plantcell.org 2005 American Society of Plant BiologistsBrassinosteroids Stimulate Plant Tropisms throughModulation of Polar Auxin Transport in Brassicaand ArabidopsisLi Li,a,b Jian Xu,c Zhi-Hong Xu,
University of Florida - HOS - 6737c
Ideally, antibiotics in tissue culture are:
University of Florida - HOS - 6737c
Chapter 6Plant Growth Regulators II:Cytokinins, their Analogues and Antagonists1. BIOLOGICAL EFFECTScomplicated by the fact that environmental factors e.g. light, water status, wounding, pathogens - maymodify responses and indeed hormone levelsthems
University of Florida - HOS - 6737c
In Vitro Cell. Dev. Biol.Plant 36:102107, MarchApril 20002000 Society for In Vitro Biology1054-5476/99 $10.00 0.00CYTOKININS: BIOSYNTHESIS, METABOLISM AND PERCEPTIONMACHTELD C. MOK*, RUTH C. MARTIN, and DAVID W. S. MOKDepartment of Horticulture and C
University of Florida - HOS - 6737c
In Vitro DevelopmentalPathwaysExplant- Piece of tissue put into culture-Tissue selection depends onpurpose, species,many factorsExplants Pieces of organs Leaves Stems Roots Cotyledons Embryos OtherExplants Specific cell types Leaf tissue
University of Florida - HOS - 6737c
ExercisesJanuary 8 Consider and discuss the anatomy of the carrot root in comparison to what you are seeing in thecultures.2,4-D is a synthetic auxin. Who first synthesized it?January 15 Identify a plant that is of interest to you that has been cultu
University of Florida - HOS - 6737c
The Florida Foliage Plant IndustryJianjun ChenMid-Florida Research and Education Center &Department of Environmental HorticultureImportant Foliage PlantsAraceaePalmaeAlocasiaAglaonemaRhapis excelsaChamaedorea elegans(Lady Palm)(Neathe Bella Pa
University of Florida - HOS - 6737c
Plant Cell Physiol. 49(11): 16451658 (2008)doi:10.1093/pcp/pcn154, available online at www.pcp.oxfordjournals.org The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.The online
University of Florida - HOS - 6737c
WhatisaPlantHormone?Materialtakenfrommanysources,butinparticular:Botanyonline:PlantHormones(Phytohormones)byPeterV.Sengbusch(www.biologie.unihamburg.de/bonline/e31/31.htm);planthormones.info,sponsoredbyMendipweb(www.planthormones.info)I.ConceptsFromth
University of Florida - HOS - 6737c
Chapter 7Plant Growth Regulators III:Gibberellins, Ethylene, Abscisic Acid, their Analoguesand Inhibitors; Miscellaneous Compounds1. GIBBERELLINS1.1. NATURAL OCCURRENCE AND PHYSIOLOGICALACTIVITYsome only in higher plants; nor are the variousgibber
University of Florida - HOS - 6737c
Applications of Somatic Hybridization andCybridization in Scion and Rootstock Improvementwith Focus on CitrusJude Grosser and Milica aloviUniversity of Florida, Citrus Research and Education Center,Lake Alfred, FL USA-Somatic Hybridization Scion Imp
University of Florida - HOS - 6737c
Plant Genetic TransformationAll stable transformation methodsconsist of three steps: Delivery of DNA into a single plant cell. Integration of the DNA into the plant cellgenome. Conversion of the transformed cell into awhole plant.Methods of delive
University of Florida - HOS - 6737c
Biotechnology,GMOs2009What is Biotechnology?The application of technology to improvea biological organism.orModification of the biological function of anorganism by adding genes (from anotherorganism?)Is biotechnology necessary?A rich source of v
University of Florida - HOS - 6737c
Plant Cell Rep (2008) 27:14231440DOI 10.1007/s00299-008-0571-4REVIEWA history of plant biotechnology: from the Cell Theoryof Schleiden and Schwann to biotech cropsIndra K. VasilReceived: 31 March 2008 / Revised: 28 May 2008 / Accepted: 10 June 2008
University of Florida - HOS - 6737c
HormoneAuxinsCytokininsGibberellinsProduct NameIndole-3-Acetic AcidIndole-3-Butyric AcidIndole-3-Butyric Acid, PotassiumSalt-Naphthaleneacetic Acid2,4-Dichlorophenoxyacetic Acidp-Chlorophenoxyacetic acidPicloramDicamba6-Benzylaminopurine6-,
University of Florida - HOS - 6737c
HOS 6373C, 2009, ProjectDo you have a favorite plant? Is there a reason to culture it in vitro or to do genetictransformation? Or is there an in vitro technique or constituent you would like to know moreabout? Exploreitinthisproject.Thisonlyhastobeavir
University of Florida - HOS - 6737c
Lab 1Establishment and Maintenance of Carrot CallusObjective: Students will sterilize carrot tissue and establish it in tissue cultureProtocol:1. In the lab, rinse a carrot root under running tap water to remove any surface soil.Make sure the root is
University of Florida - HOS - 6737c
Lab 2Media PreparationObjective: Students will prepare media for an upcoming experimentCauliflower mediumMS saltsMyo-InositolGM stockSucroseKinetin (stock)Add per 1 Liter4.3g (1 packet)100mg10ml30g2.5 mg/LAdjust pH to 5.6, bring to volume i
University of Florida - HOS - 6737c
Lab 3aProduction of Plantlets from Flower Organs of CauliflowerProtocol:1. Outside of the hood, select a clean floret from a fresh cauliflower head. Usingforceps and scalpel, trim off 12-15 small pieces of florets for explants2.In the flow hood, tra
University of Florida - HOS - 6737c
Lab 4Shoot Tip Cultures of Sweet & White PotatoProtocol:1. Cut off four shoot tips from the Sweet Potato shoots.2. Wash the tips in Clean water.3. Place in a clean beaker and wash in 70% EtOH for 30 seconds ONLY. Pour offEtOH.4. Wash in 2% bleach w
University of Florida - HOS - 6737c
Lab 5Micropropagation of Anthurium andreanumProtocol:1. Cut off four shoots from the Anthurium plant. Trim off leaves.2. Wash the tips under tap water to remove debris.3. Place in a clean beaker and wash in 70% EtOH for 1 minute. Pour off EtOH.4. Wa
University of Florida - HOS - 6737c
Lab 6Control of organogenesis in cultures of Nicotiana tabacumObjective: To observe the effects of various levels of hormones on tobacco explants.Protocol:1. You will be given a container with several tobacco seedlings. The seedlings havebeen grown f
University of Florida - HOS - 6737c
Lab 7Tobacco Transformation1. Start Agrobacterium culture to be used from a glycerol stock. Grow up overnight at 28C,under the proper selection. We will do this for you.2. Tobacco has been grown aseptically. On the next day cut the leaves off the toba
University of Florida - HOS - 6737c
Lab 8Transgenic Arabidopsis1. You will be given a tube with a population of Arabidopsis seed.2.Using a pipet, add 1ml of the bleach solution (1.5% bleach, 0.02% (v/v) Triton X-100)3. Put the tube on the shaker for 20 minutes at room temp.4. Allow th
University of Florida - HOS - 6737c
Lab 9Reporter GenesGUSThe GUS gene is used in transgenic studies to verify the transmission of thetransformation vector into the target plant. It is informative because nothing in the planttissue would stain blue; the blue stain can only mean the pla
University of Florida - HOS - 6737c
Lab 10Electrophoresis50X TAE electrophoresis buffer:242g Tris Base57.1ml Glacial Acetic Acid100ml 0.5M EDTA (pH 8)1L totalProcedure:1. Make 1L of 1X TAE buffer (provided).2. Mix 1g agarose with 100ml 1X TAE buffer in a 125ml Erlenmeyer flask. Cov
University of Florida - HOS - 6737c
LABSAFETY1.Apparel:Shoesaretobewornatalltimes.Itisbestnottowearopentoedshoesorsandals,sincetheyoffernoprotectionfromspills.Wewillbeusingsubstancesthatcanruinyourclothes,e.g.bleach,soifyouhavealabcoat,youmaywanttowearit.Wewillnotbeusingmanydangerousche
University of Florida - HOS - 6737c
LAB SAFETYApparel Shoes Lab coats Safety glassesNote!No eating, drinking, or smokingin the lab at any time.Spills Wash Notify Receive instructionsAccidents Be careful! Pay attention Report any injuriesOther MattersBroken glassOther waste
University of Florida - HOS - 6737c
PhytoTechnology Laboratories, Inc.Product Information SheetDedicated to a BetterWay of Life throughTMPlantsMEDIA PREPARATION FROM BASAL SALT SOLUTIONSLiquid 10X solutions are offered for your convenience. To avoid precipitation over long-termstora
University of Florida - HOS - 6737c
PhytoTechnology Laboratories, Inc.Product Information SheetDedicated to a BetterWay of Life throughTMPlantsMedia Preparation From Powdered MediaPowdered media are extremely hygroscopic and must be protected from atmospheric moisture.If possible th
University of Florida - HOS - 6737c
Plant Tissue CultureW hat is it? Tissue culture is the term used forthe process of growing cellsartificially in the laboratory Tissue culture produces clones, inwhich all product cells have the samegenotype (unless affected bymutation during cultu
University of Florida - HOS - 6737c
Chapter 3The Components of Plant Tissue Culture Media I:Macro- and Micro-Nutrients1. INORGANIC MEDIUM COMPONENTSformulation. These media result often in a muchimproved growth (Rugini, 1984; El Badaoui et al.,1996; Pullman et al., 2003; Bouman and Ti
University of Florida - HOS - 6737c
Chapter 4The Components of Plant Tissue Culture Media ll:Organic Additions, Osmotic and pH Effects,and Support Systems1. ORGANIC SUPPLEMENTSthiamine, in media published by Bonner (1940),Gautheret (1942) and White (1943b); this wasfollowing the find
University of Florida - HOS - 6737c
PhytoTechnology Laboratories, Inc.Product Information SheetDedicated to a BetterWay of Life throughTMPlantsPLANT TISSUE CULTURE MEDIA PREPARATIONFormulation charts posted on the "Technical Literature" page on web siteFROM PACKED POWDERPowdered me
University of Florida - HOS - 6737c
1Our Plant Tissue Culture Tested basal salts and mediahave been formulated according to the referencescited in their respective product listings. These basal saltsand media are then manufactured and packaged in environmentally controlled facilities.
University of Florida - HOS - 6737c
NutrientMediaforPlantTissueCulturesOneofthefirstdecisionsthatmustbemadewhendevelopingatissueculturesystemiswhatmediumtouse.Nutrientmediaforplanttissueculturearedesignedtoallowplanttissuestobemaintainedinatotallyartificialenvironment.Manydifferenttissue
University of Florida - HOS - 6737c
11Meristem-Tip Culturefor Propagation and Virus EliminationBrian W. W. Grout1. IntroductionThe essence of meristem-tip culture is the excision of the organized apexof the shoot from a selected donor plant for subsequent in vitro culture. Theconditi
University of Florida - HOS - 6737c
Micropropagation the art andscience ofmultiplying plantsin vitro.Plant Propagation by TissueCultureGJ DeKlerk, EF George, MA Hall (eds)3rd Edition, 2008Springerhttp:/www.springerlink.com/content/n5tm30/?p=2d1b86aedb5a437f987c7bd63962bbc8&pi=0
University of Florida - HOS - 6737c
MODIFIED SHOOT TIP MEDIA 1 literLiquid with paper bridge1 Pkg M-S salts with Vitamins(Thiamine Pyridoxin HCl Nicotinic acidi-Inositol) Sold as 1 L. envelope fromPhytotechnologies Lab Inc.75 grams SucroseCombine above ingredients in 1 L deionizedH2