Problem Set 3_answers
4 Pages

Problem Set 3_answers

Course Number: BIOL 313, Spring 2008

College/University: Iowa State

Word Count: 748

Rating:

Document Preview

Problem Set 3 Biology 313, Principles of Genetics Due Monday March 10, 2008 Name____________________________________________ 1) (3 pts) (1A) How are heterogeneous nuclear RNA (hnRNA) and messenger RNA (mRNA) related? hnRNA is the primary transcript that is processed into mRNA; hnRNA is pre-mRNA; hnRNA is processed into mRNA. (1 pt) (1B) Where in the cell would each type of RNA be found? hnRNA - nucleus mRNA...

Unformatted Document Excerpt
Coursehero >> Iowa >> Iowa State >> BIOL 313

Course Hero has millions of student submitted documents similar to the one
below including study guides, practice problems, reference materials, practice exams, textbook help and tutor support.

Course Hero has millions of student submitted documents similar to the one below including study guides, practice problems, reference materials, practice exams, textbook help and tutor support.

Set Problem 3 Biology 313, Principles of Genetics Due Monday March 10, 2008 Name____________________________________________ 1) (3 pts) (1A) How are heterogeneous nuclear RNA (hnRNA) and messenger RNA (mRNA) related? hnRNA is the primary transcript that is processed into mRNA; hnRNA is pre-mRNA; hnRNA is processed into mRNA. (1 pt) (1B) Where in the cell would each type of RNA be found? hnRNA - nucleus mRNA nucleus &/or cytoplasm (1 pt) (1C) List the 3 major processing events required to make mRNA? Addition of 5' cap Addition of poly-A tail Splicing of introns and ligation of exons (1 pt) 2) (4.5 pts) The nontemplate strand of DNA has the following base sequence: 5'-CGCGGTTAGGTATAATTGACGCGTATGAGAGCTGTTCTGCTCCTTTTTAGTAAACCTTAATGAGCT-3' (2A) Underline in the above sequence an important cis-acting promoter element and provide its name. -10 region or Pribnow box; also accepted TATA or Goldberg-Hogness box. (0.5 pt) (2B) Find the open reading frame and write the corresponding mRNA sequence starting with the 5' codon. The first START codon (ATG) is shaded, which sets the reading frame. Every 3 bases are read until the 1st in-frame STOP codon (underlined) is reached. mRNA = (1 pt) AUG-AGA-GCU-GUU-CUG-CUC-CUU-UUU-AGU-AAA-CCU-UAA 1 (2C) Translate this mRNA into a sequence of amino acids. mRNA = protein = (1 pt) AUG-AGA-GCU-GUU-CUG-CUC-CUU-UUU-AGU-AAA-CCU-UAA MET-ARG-ALA-VAL-LEU-LEU-LEU-PHE-SER-LYS-PRO-STOP (2D) Assume a single C is inserted in the above mRNA sequence between the second and third base of the triplet codon for the first Phe. Write the new mRNA strand that results from this insertion and its translation product. What is the name for this type of change? mRNA = protein = (1 pt) AUG-AGA-GCU-GUU-CUG-CUC-CUU-UUC-UAG MET-ARG-ALA-VAL-LEU-LEU-LEU-PHE-STOP frameshift mutation (1 pt) 3) (2.5 pts) Below is a drawing which represents simultaneous transcription and translation in E. coli. Supply the correct response for the questions provided below. The direction of the RNA polymerase is given by the arrow. (0.5 pt each) (3A) The letter A is nearest the 5' or 3' end of the molecule? State which ____3'____ (3B) The letter B is nearest the 5' or 3' end of the molecule? State which. ___5'_____ (3C)The letter C is nearest the 5' or 3' end of the tRNA molecule? State which. ___3'_____ (3D) What is the "S" value for the large rRNA which is closest to the letter D? ___23S_____ (3E) Which terminus (N or C) of the growing polypeptide chain is nearest the letter E? ___N_____ 2 4) (3 pts) Below is a table showing the effect of different on media the growth of different tryptophan mutations in Salmonella typhimurium (+ = growth, - = no growth) Strain trp-8 trp-2 trp-3 trp-1 No Supplement - Medium Supplemented with IGP AA IN TRY + + + + + + + + + + (4A) Construct the biochemical pathway for the compounds IGP, AA, IN, and TRY based on these data. X >>>>> AA >>>>>>> IGP >>>>>>> IN >>>>>> TRY (1 pt) (4B) Place strains of bacteria (mutations) in the appropriate steps in the above pathway. trp-8 trp-2 trp-3 trp-1 X >>>>> AA >>>>>>> IGP >>>>>>> IN >>>>>> TRY (1 pt) (4C) In bacteria it is often possible to make partial diploid strains. Assume that a diploid strain was made containing the complete genomes of the trp-2 and trp-1 strains. Would this diploid strain be able to grow on the unsupplemented medium? Yes or no ? _YES_______ Explain your answer. The trp-2- mutant strain has a functional trp-1+ gene and the trp-1- strain has a functional trp-2+ gene. As a diploid, the 2 mutant genes would be complemented by the functional versions from each strain. (1 pt) 5) (1 pt) In a survey of 240,000 human births, six achondroplastic births were recorded to parents who were unaffected. Given that this form of dwarfism is caused by a fully penetrant, dominant, autosomal gene, what is the mutation rate? 6 = 6 = 1.25 x 10-5 (2) 240,000 480,000 The mutation rate = (the number of mutant gametes)/(the total number of gametes surveyed) (1 pt) 6) (1 pt) Describe how acridine dyes cause frameshift mutations. Acridine dyes intercalate between the bases of intact double stranded DNA. (1 pt) 3 7) (4 pts) The table below lists several genotypes associated with the lac operon in E. coli. For each genotype, indicate with a "+" or a "-" whether -galactosidase would be expected to be produced both on media with "no lactose" and on media "with lactose". -galactosidase production No Lactose With Lactose Genotype (7A) (7B) (7C) (7D) I+ O+ Z+/ F' I- O+ Z+ I- Oc Z+/ F'I- O+ ZIs Oc Z+/ F'I+ O+ Z+ I- O+ Z+/ F' I- O+ Z+ + + + + + + + (1 pt each genotype) I+ = wild type repressor I- = mutant repressor (unable to bind to the operator) Is = mutant repressor (insensitive to lactose) O+ = wild type operator Oc = constitutive operator (insensitive to repressor) 8) (1 pt) Given the diagram below, what type of control, positive or negative, is operating? Positive a regulatory protein binds and transcription occurs. (1 pt) 4

Find millions of documents on Course Hero - Study Guides, Lecture Notes, Reference Materials, Practice Exams and more. Course Hero has millions of course specific materials providing students with the best way to expand their education.

Below is a small sample set of documents:

Iowa State - ANSCI - 224
Animal Science 224 Study Guide What do these terms mean? Opportunist like crows, found a niche around human environment (can live in extremes) Mutualistic specis that is wild but interact with other species Domestic animals from wild, and altered
Iowa State - ANSCI - 224
How often should you breed a bitch? Once every 2 years, (every other cycle at most) How often should you breed a stud? Want to avoid overuse because of genetic disease if to many population Won't have time to evaluate offspring What causes infertili
UNC Asheville - ENGLISH - 101
Tim Hoskins 2nd DraftAll heroes have a personal life away from their hero life. Finding what is close and personal to a hero, and hurting it, is key for all villains. In the novel Frankenstein this is what the monster does to his creator Victor Fra
Virginia Tech - AOE - 3104
Homework #1 Solution1. One side of the manometer is attached to the test item while the other is vented to the atmosphere. The pressure difference, the test value of gauge pressure, is manifested in the height differential in the water manometer. Pm
Virginia Tech - AOE - 3104
AOE 3104 Homework #2 SolutionsProblem 1. In a wind tunnel, the speed changes as the cross sectional area of the tunnel changes. If the speed in a circular test section with radius 5 feet is 100 miles per hour, what is the speed upstream of the test
Virginia Tech - AOE - 3104
AOE 3104 Homework #3Assigned: February 4, 2008 Due: February 11, 2008 (Place your homework in the box outside my office by 5 PM.) Note to the Students: You can find Standard Atmosphere Tables on p.11 and p.12 of your Marchman texts. Also, in this so
Virginia Tech - AOE - 3104
AOE 3104 Homework #4 SolutionsProblem 1. Consider an airfoil in a 50 m/s air flow at standard sea level conditions. At a point on the airfoil, the pressure is 9.5 104 Pa. What is the pressure coefficient at this point? (Please use SI units for the
Virginia Tech - AOE - 3104
AOE 3104 Homework #5 SolutionsProblem 1. A jet-propelled airplane has a parabolic drag polar with the following parameter values: CD0 = 0.024, A = 7, R e = 0.85, S = 120 m2 , m = 75, 000 kg, TSL = 150, 000 N.Assume that T (h) = TSL (h) = TSL (h) .
Carnegie Mellon - HIST/MATH - 79-104 and
World History Lecture Notes 10/1/07 Lecture Outline: I.) How should we approach witchcraft accusations-confessions? 1) Get details right a.) time-place b.) accusers-accused-accusations c.) responses-accused; others 2) contextualize 3) consider simila
Carnegie Mellon - HIST/MATH - 79-104 and
World History Lecture Notes 10/8/07 I.) II.) III.) What were the main features of the African slave trade. How should we interpret them? How does marks interpret the African slave trade trade cites geo-politcal implications? How does Wolf interpret t
Carnegie Mellon - HIST/MATH - 79-104 and
World History Lecture Notes- 10/10/07 I.) How is the first settlement of what is now the US most commonly represented? II.) What were the main strategies used by English capitalists and their allies to create and consolidate transatlantic capitalism?
Carnegie Mellon - HIST/MATH - 79-104 and
World History Lecture Notes 10/3/07 Proposition to be Debated: Silverblatt's book and our discussion of it in class offer a persuasive alternative to: (a) dominant accounts in the United States of the Incas and the Spanish conquest and (b) the ways t
Carnegie Mellon - HIST/MATH - 79-104 and
World History Lecture Notes 11/5/07 I) What general factors were most significant in producing mass deaths from famine in India and China, from 1876-1902, and the growing gap between the west and the rest A) Marks B) Davis C) 1873 and capitalist cris
Carnegie Mellon - HIST/MATH - 79-104 and
Lecture Notes 11/14/0711/14/2007 12:35:00 PM I) What should we make of the US intervention in Iraq? Khaldi argues that the Bush administrations reasons for invasion- Sadaam Hussein's regime had weapons of mass destruction. > Even before the invasio
Carnegie Mellon - HIST/MATH - 79-104 and
12/3/07 Lecture NotesI) Where are we now? 1) Issues 2) Links and Dynamics 3) globalization 4) A new systemic crisis II) What can we learn from past crises? 1) 1910s + 2) 1940s + 3) 1970s+12/3/2007 12:30:00 PMIII) Where might we be heading & what
Carnegie Mellon - HIST/MATH - 79-104 and
World History Lecture Notes- 10/29/07 I) What is Davis's book about? 1) causes 2) responses of powerful 3) reactions of most vulnerable groups 4) effects 5) lessons for present II) How does Davis define his key terms? 1) El Nino 2) 3) Political ecolo
Carnegie Mellon - HIST/MATH - 79-104 and
World History Lecture Notes 9/12/07 Situating Ourselves The Global Garment Industry I.) II.) III.) What do we know about the clothes we're thinking of buying/have bought? What else do we need to know? How can learning more help us? A) Engage contemp
Carnegie Mellon - HIST/MATH - 79-104 and
Turning to World History- Lecture 9/17/07 I.) What is involved in engaging the world through (world) history? II.) How are we going to do it? III.) How does Marks do it? Consciously/unconsciously Engaging = interpreting + acing Evaluating understand
Carnegie Mellon - HIST/MATH - 79-104 and
Reading Marks World History Lecture 9/19/07 I.) How should we approach World History? 1.) Start in the present 2.) Use history to explain 3.) Identitfy and appraise dominant narr. 4.) constuct alternative II.) What was the world like in the 15th cen
Carnegie Mellon - HIST/MATH - 79-104 and
Notes for Late Victorian Holocaust Preface -> Severe drought from 1876-1879 over much of Asia, impact was famine Ulysses S. Grant and his family took an extravagant vacation all over Europe, were reported for Herald readers by John Russel Young. Whi
Carnegie Mellon - HIST/MATH - 79-104 and
The Role of the GovernmentThe general functions of the government are to: regulate law and order * restrict monopolies * minimal intervention"The scope of the government must be limited. Its major function must be to protect our freedom both from
Carnegie Mellon - HIST/MATH - 79-104 and
Math 2.311/8/2007 12:33:00 PM Section 2.3) Power Rule, Linearity (no chain rule or product rule, quotient Rule) 16) Find dy/dx when y= (x-1)power rule d/dx (x^a ) = ax ^ a-1 Linearity d/dx (f+g) = df/dx + dg/dx d/dx y= = d/dx x^1/2 = 1/2x ^1/2 (x
University of Michigan - PSYCH - 250
Framework and Theories of DevelopmentLecture 1 January 8, 2008Developmental Psychology: The Lifespan Perspective What is development? A process of agerelated changes across the lifespan. Six assumptions of the lifespan perspective: 1. Developme
Rutgers - PSYCH - 01:830:338
Bhavin Patel Review Sheet For Personality Final Exam Page |1Stuff on Heidegger Smart man, but George would never want to be his friend! How does Heidegger's philosophy relate to the Newtonian-Galilean Weltanschauung? It is against the NGW and opera
University of Michigan - PSYCH - 250
Genetic Basis of DevelopmentLecture 2 January 10, 2008Overview Goal: to understand what genes will and will not tell us about developmental psychology Basic terminology and concepts Patterns of genetic inheritance Video on the Human Genome Pro
Rutgers - ACCT - 33:010:272
PREVIEW OF CHAPTER 1Accounting in ActionWhat is Accounting?The Building Blocks of AccountingUsing the Building Blocks Financial Statements Who uses accounting data? Brief history of accounting Bookkeeping and accounting Accounting and
Rutgers - PSYCH - 01:830:338
WORLDS OF EXPERIENCE NOTES Cartesian Doctrine of the Isolated Mind: o Divides the subjective world of the person into outer & inner regions o Pictures the mind as an objective entity that takes its place among other objects; a "thinking thing" that h
Rutgers - PSYCH - 01:830:338
Chapter 3: Carl Jung First to recognize the problem of the subjectivity of personality theory. Observed conflict between Freud and Adler about human neurosis and wondered how it was that each investigator saw only one side, and why each maintained th
Rutgers - PSYCH - 01:830:338
Personality Psychology Final Exam Review Sheet How does Heidegger's philosophy relate to the Newtonian-Galilean Weltanschauung?- Heidegger`s philosophy`s good points gets attacked for his life`s bad points: ad hominem argument - toward man -> aka cri
Rutgers - BIO - 100
Erick Liang Biology Concepts 100 2/27/08 Data Analysis and Spreadsheets I. Pre-Lab Preparatory Information A. Spreadsheets i. Graph and calculate functions, and provide visual presentation of patterns, along with summary or descriptive statistics and
University of Michigan - PSYCH - 250
Prenatal DevelopmentLecture 3 January 15, 2008Overview Goal: To describe the events that lead from the formation of a single cell (conception) to an amazing newborn baby Stages of prenatal development Germinal, Embryonic, Fetal Focus on brain
Rutgers - BIO - 100
Erick Liang Biology 100 2/19/08Sciences and MusclesI.II.Pre-Lab Preparatory Information A. The Scientific Method verifying if a hypothesis is correct 1. The Problem or Observation 2. Collection of Background Information 3. State your Hypothe
Rutgers - BIO - 100
Erick Liang Biology 100 2/13/08Central Nervous SystemI.II.Pre-Lab Information A. Nervous system tissues and organs that allow person to respond internally and externally to environment. 1. sensing, integration, effect 2. Two parts central n
Rutgers - BIO - 100
Erick Liang Biology 100 2/4/08Anatomy and HistologyI.II.Pre-lab Preparatory Information A. Orientation terms- posterior (side farthest away) and anterior (side toward front) B. Bilateral symmetrical (one equally dividing plane along midline),
Rutgers - BIO - 100
Erick Liang Dr. Transue Biology Concepts 100 1/27/08 Microscopy Goal: Ensure one can use compound microscope effectively and safely I. Background Info About Microscopy A. History, uses, and types of microscopes are presented. Terms related to microsc
ASU - EEE - 304
Midterm SolutionSolution by Harvey Thornburgs+4 1. (a) Factor H(s) = (s+1)(s+2) . There are two poles: s = -1 and s = -2, and only one zero: s = -4.(b) Possible ROC is: Re{s} > -1 -2 < Re{s} < -1 Re{s} < -2 As Re{s} > -1 contains the j-axis,
University of Michigan - PSYCH - 250
Birth & Infant Physical, Perceptual and Motor DevelopmentLecture 4 January 17, 2008OverviewBirth Stages of labor Video The amazing newborn Physical condition Reflexes Senses States sleeping and crying Feeding Infancy Body growth M
ASU - EEE - 203
University of Michigan - PSYCH - 250
Infant Cognitive and Language DevelopmentLecture 5 January 22, 2008Reminder Exam 1 next Tuesday Sixty questions (each worth 1 point) Two bonus questions Review sheet posted on Thursday night Sample questions posted on Thursday night Also, Th
UT Dallas - GEOLOGY - 3321
KarstKarst features include sinkholes, caves, springs, sinking streams, cavities, dissolutionenlarged joints and/or bedding planes, and cutter-pinnacle zones, not all of which may be present or obvious.http:/www.technos-inc.com/geomethods.htmlKa
UT Dallas - ISNS - ISNS 3359
1. Location 4: 50 km (30 miles) SSW of Kalamata, Greece, 36.646N, 21.833E 2. Magnitude: 6.9 Mw 3. Date-Time: Thursday, February 14, 2008 at 10:09:23 UTC; 12:09 PM (local time) 4. Hypocenter Depth: 29 km 5. Plate Tectonic Setting: The epicenter is loc
Rutgers - SOC - 283
2/26Check sakai Friday for paper topic and have by next thurs have assignment question Done on sakai Look up why voters care again-time CooleyAny system of ideas drawn from life tat he uses as his own and that we get from communicating with people.
UT Dallas - STAT - STAT 3360
Descriptive StatisticsNumerical Summaries Chapter 4Stat 33601Chapter 4 Numerical summaries Population versus sample Central tendency-location Variation-Spread Graphical displays of dispersion CovarianceStat 33602Descriptive Stati
UT Dallas - STAT - STAT 3360
Statistics For Management and EconomicsChapter 1 Chapter 21"There Are 3 Kinds of Lies Lies, Damned Lies and Statistics."Mark Twain2Everyday Statistics Examples Political polls the candidate has a 55% approval rating plus or minus 3% The
Tyler College - CHEM - 1411
Periodic Table Exam stuffHHeLiBeBCNOFNeNaMgAlSiPSMgClArKCaScTiVCrMnFeCoNiCuZnGaGeAsSeBrKrRbSrYZrNbMoTcRuRhPdAgCdInSnSbTeIXeWPtAuHg
Tyler College - PSYC - 2301
Psychology is a study that through the years has been proven to show how our minds and behavior reflect one another and just how science is involved. Though psychology can bring about many controversial issues without the debates we would never learn
Dickinson - ECON - 111
Rutgers - SOC - 283
Lecture Notes 11/13/071thermoregulation -ectotherms- take temp of envir, -endotherms what we are we maintain temp, -heterotherms - goes with thermodynamics - why is it when you exercise you warm up heat is converted, 2nd law - we are constantly f
Dickinson - ECON - 111
Dickinson - ECON - 111
Microeconomics 111 February 10, 2008 Homework 2 6. a) The supply of pizzas have significantly decreased, creating a shortage in the number of pizzas available on the market, driving up the price to compensate for the increased marginal benefit. b) Th
Dickinson - INBM - 100
INBM 100 Fundamentals of Business Fall 2007 12:30PM-1:20PM MWF Quiz Week 1 Time allowed 20 minutes (1) A successful business is one that: (a) (b) (c) (d) Has the highest volume of sales Charges the highest price Has been around for a long duration
Dickinson - INBM - 100
Chapter ObjectivesSlide 7 - 2List the major economic forces beyond the immediate control of a business that affect the firm's success. Use simple macroeconomic concepts to explain how growth, inflation, unemployment, and interest rate changes occu
Rutgers - SOC - 283
3/11/08 Review Essay: talk very specifically abt boundary issues. Where a boundary was drawn, how and why. Examples from class. Research issue maybe. Read Ann Stoler Article Give supporting evidence NY Times article on Sakai~ EC questions that also r
Rutgers - SOC - 283
3/26 Berger (worked at rutgers) and luckmann p. 119 We create society, society creates individuals Society vs. Unsuccessful civilization Society best understood as "an ongoing dialectical process composed of the 3 moments of (Major themes:) externali
Blinn College - ART - 1301
HEN IT RAINS, I DON'T MIND BEING LONELY, I CRY RIGHT ALONG WITH THE SKY WHEN IT RAINS, I DON'T PRETEND TO BE HAPPY, I DON'T EVEN HAVE TO TRY WHEN IT RAINS, I DON'T MIND BEING LONELY, I CRY RIGHT ALONG WITH THE SKY WHEN IT RAINS, I DON'T PRETEND TO BE
Rutgers - BIO - 101
Jessica Parrotta Lecture Notes11/13/07I.Thermoregulation A. Ectotherms (ecto= outside) 1. take temperature of environment B. Endotherms 1. what we are, because we maintain a stable temperature and rate of diffusion C. Heterotherms 1. hetero=diffe
Texas A&M - ENGR - 112
April 6, 2008MemorandumTo: From: Dr. O Team # [names]Subject:Ross Street Reconstruction Project, Final MemoSummary: We did an initial site inspection to assess the magnitude of the damage of Ross Street. We found that there was a pipeline fa
Virginia Tech - LDRS - 1016
Reading 1 The complexity of identity "Who Am I?"Many dimensions: social context, gender, age, wealth, sex. Orientation, religion, others view of you Erik Erikson(termed identity crisis): social, cultural, and historical context forms identity -its o
Virginia Tech - LDRS - 1016
Reading 6?Fresh Lipstick: Rethinking Images of Women in Advertising -Over 100 years feminists believe thought of beautiful appearance= low self-esteem and is a symptom of political oppression -causes problems because must look against the beauty, l
Texas A&M - ENGR - 111
A mechanism is a collection of links connected together and restrained in such a way that they carry out a desired motion. They can be thought of as under constrained or unstable trusses Unlike a truss that is designed to be rigid, a mechanism is
Virginia Tech - LDRS - 1016
Reading 4Moving beyond white guiltHow we can defuse the negative aspects of guilt -get info: learn about racism (live free of blinders and be filled with true self-respect) -do something: antidote to guilt is action -listen: listen when someone of