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10. Chromatin I
Course: BIO 344, Spring 2011School: University of Texas
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Nuclease Micrococcal digestion of bulk chromatin What does this tell you? Gene Regulation in prokaryotes vs. eukaryotes In eukaryotes, the ground state is restrictive What is the silent state? RNAP cant assemble on promoters packaged->chromatin What is the poised state? Basic chromatin structure in growing cells Fig 4-22 MBofC5 Figure 04-05 DNA is highly electronegative. What does this suggest...
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Nuclease Micrococcal digestion of bulk chromatin
What does this tell you?
Gene Regulation in prokaryotes vs. eukaryotes
In eukaryotes, the ground state is restrictive
What is the silent state?
RNAP cant assemble on
promoters packaged->chromatin
What is the poised state?
Basic chromatin structure in growing cells
Fig 4-22 MBofC5
Figure 04-05
DNA is highly electronegative. What does this suggest
about histones and their role in DNA compaction?
Histones are post-translationally modified. This histone code
overlays on top of the genetic code and directs packaging and access to
the genome
Figure 04-12
Figure Molecular 4-38 Biology of the Cell ( Garland Science 2008)
Zigzag model for 30nm fiber
Condensation of nucleosomes->30 nm fiber requires histone H1
Xray crystal structure based on a tetrasome
Solenoid model for 30 nm fiber based on cryo-EM
Nucleosome positioning: Are histones randomly
distributed across genes or are they phased?
Chromatin Immunprecipitation and microarrays
Limited in resolution and/or scope
Genome-scale fine mapping identifies general
rules of nucleosome positioning
NextGen Sequencing
There are several different
techniques. This is Solexa (or
Illumina) sequencing
movie
NextGen Sequencing
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University of Texas - BIO - 344
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University of Texas - BIO - 344
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University of Texas - BIO - 344
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University of Texas - BIO - 344
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University of Texas - BIO - 344
University of Texas - BIO - 344
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UCLA - CHEM - 113A
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UCLA - CHEM - 113A
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University of Texas - MATH - M 408K
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