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Chapter 16: Recombinant DNA and Biotechnology TEST FILE QUESTIONS Multiple Choice 1. Which of the following enzymes are used by bacteria to defend themselves against bacteriophage? a. DNA polymerase b. Reverse transcriptase c. Phosphofructokinase d. Restriction endonuclease e. None of the above Textbook Reference: 16.1 How Are Large DNA Molecules Analyzed? p. 353 2. Most restriction sites are _______ base pairs long. a. 1 or 2 b. 46 c. about 10 d. about 20 e. over 50 Textbook Reference: 16.1 How Are Large DNA Molecules Analyzed? p. 353 3. Which of the following is true about restriction enzymes? a. They cut at regular intervals, every 4,000 base pairs. b. They prefer to cut at DNA that has been methylated. c. Eco RIs restriction site is a palindrome. d. All of the above e. None of the above Textbook Reference: 16.1 How Are Large DNA Molecules Analyzed? p. 354 4. Which of the following enzymes would a bacterium most likely use to prevent its DNA from being chopped up by its own restriction enzymes? a. DNA polymerase b. Ligase c. Lactase d. Reverse transcriptase e. Methylase Textbook Reference: 16.1 How Are Large DNA Molecules Analyzed? p. 354 5. DNA is _______ charged due to the presence of a _______ group. a. negatively; methyl b. negatively; phosphate c. negatively; carbon d. positively; methyl e. positively; phosphate Textbook Reference: 16.1 How Are Large DNA Molecules Analyzed? p. 354 6. DNA, because it has a _______ charge, moves to the _______ end of the field in gel electrophoresis; _______ DNA molecules migrate the most quickly. a. positive; positive; smaller b. positive; positive; larger c. positive; negative; smaller d. negative; positive; larger e. negative; positive; smaller Textbook Reference: 16.1 How Are Large DNA Molecules Analyzed? p. 354 7. Which of the following would most likely involve a nylon filter? a. Restriction digest b. Genetic knockout c. Southern blot d. Creation of artificial chromosomes e. Construction of a gene library Textbook Reference: 16.1 How Are Large DNA Molecules Analyzed? p. 354 8. Southern blotting is used a. to prevent restriction enzymes from inappropriately digesting DNA. b. to reveal specific DNA sequences. c. to make genetic knockouts. d. in pharming. e. to transfect cells. Textbook Reference: 16.1 How Are Large DNA Molecules Analyzed? p. 354 9. Which of the following would most likely involve radioactively labeled, single- stranded DNA? a. Restriction digestion b. Pharming c. Constructing a gene library d. Southern blotting e. None of the above Textbook Reference: 16.1 How Are Large DNA Molecules Analyzed? p. 354 10. Restriction enzymes cleave DNA at specific sequences by hydrolyzing a. the 3 hydroxyl of one nucleotide and the 5 phosphate of the next one.... View Full Document

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