Course Hero has millions of student submitted documents similar to the one
below including study guides, practice problems, reference materials, practice exams, textbook help and tutor support.
Find millions of documents on Course Hero - Study Guides, Lecture Notes, Reference Materials, Practice Exams and more.
Course Hero has millions of course specific materials providing students with the best way to expand
their education.
Below is a small sample set of documents:
Broward College - MCB - MCB2010
METABOLICDIVERSITYAnorganismsmetabolicpathwaysdependontheenzymesavailable.Allorganismscanbeclassifiedaccordingtotheirnutritionalpattern:1.Sourceofenergy2.Sourceofcarbon1.ENERGYSOURCEa.Phototrophscanuselightenergyb.Chemotrophsmustobtainenergyfromoxi
Broward College - MCB - MCB2010
METABOLICREACTIONSMetabolismsumofallthechemicalreactionswithinalivingorganism.2maintypesofreactionsareinvolved:1.Catabolism(catabolicordegradationreactions)thesebreakdownlargermoleculestosmallerones,breakingchemicalbondsandreleasingenergyintheprocess(
Broward College - MCB - MCB2010
METHODSOFCLASSIFYINGANDIDENTIFYINGMICROORGANISMSFATTYACIDPROFILESoftenthetypeandamountofvariousfattyacidsarecharacteristicofcertainspeciesofbacteria.Cellularfattyacidscanbeseparatedandcomparedtoknownprofiles.FLOWCYTOMETRYfluidcontainingbacteriaisforc
Broward College - MCB - MCB2010
METHODSOFCLASSIFYINGANDIDENTIFYINGMICROORGANISMSSEROLOGYhisisthesciencethatstudiesbloodserumandimmuneresponsesthatcanbemeasuredinserum.Whenmicroorganismsenterthebody,oneoftheresponsesistheformationofproteinscalledantibodies.Antibodiescirculateintheblo
Broward College - MCB - MCB2010
METHODSOFCLASSIFYINGANDIDENTIFYINGMICROORGANISMSMicroorganismsareidentifiedandclassifiedformorethanonereason.Weattempttoidentifyandclassifyalllivingthings,butdeterminingtheidentityofpathogensisespeciallyimportant,becauseitoftenmakeseffectivetreatmentp
Broward College - MCB - MCB2010
METHODSOFCLASSIFYINGANDIDENTIFYINGMICROORGANISMSRIBOSOMALRNASEQUENCINGthisisanothertechniqueusedtodeterminetherelationshipsamongorganisms.RibosomalRNAisbelievedtochangelessthanothernucleicacidsovertime,becauseifanorganismhasadrasticchangeinitsrRNAitwo
Broward College - MCB - MCB2010
MICROBIALGENETICSGeneticsisthescienceofheredity.Itincludes:WhataregenesHowdotheycarryinformationHowaretheyreplicated(copied)HowaretheypassedtosubsequentgenerationsofcellsHowaretheypassedfromorganismtoorganismHowdoestheirexpressiondeterminethecharac
Broward College - MCB - MCB2010
MICROBIALGENETICSWhileageneisbeingusedasapattern,thetwostrandsofDNAmustbeseparated.Thehydrogenbondsthatlinkthestrandsareweakandeasilybroken.ThetwostrandsofDNAmustalsoseparatewhentheDNAisreplicatedpriortocelldivision.DNAcanchange(mutate).Thisisoftendam
Broward College - MCB - MCB2010
DIRECTMEASUREMENTOFMICROBIALGROWTHa.POURPLATESTomakeapourplate,generallyeither1mlor0.1mlofthedilutionisplacedinasterileemptyPetridish.Nutrientagarwhichhasbeenheldinliquidformatabout45oCisaddedtothePetridishandcarefullymixed.Theagarquicklysolidifiesandb
Broward College - MCB - MCB2010
MITOCHONDRIAthesearethepowerhouses,wheremostofthecellsATPisproduced.Amitochondrionhas2membranes,asmoothoutermembraneandaninnermembranethatliesindeepfoldscalledcristae.Theinnercavityisthematrix.Theprocessofcellularrespirationiscarriedoutbyenzymesassocia
Broward College - MCB - MCB2010
Modern Developments in Microbiologyvarious branches of microbiology have developed:o bacteriology study of bacteriao mycology study of fungio parasitology study of protozoa and parasitic wormso immunology study of how immunity reacts to various patho
Broward College - MCB - MCB2010
MONOCLONALANTIBODIESInadditiontobodydefense,antibodiesarealsoveryhelpfulindiagnosisofdisease.Forsometime,theprocedureforobtainingtheminvolvedinjectionoftheantigenintoananimal,whichstimulatedtheanimaltoproduceantibodieswhichcouldberecoveredfromtheserum.
Broward College - MCB - MCB2010
MULTIPLICATIONOFANIMALVIRUSESThisprocessfollowsthesamebasicpatternasbacteriophagemultiplication,butthereareseveraldifferences.Hereisabriefcomparison:STAGEBACTERIOPHAGEANIMALVIRUSESATTACHMENTTAILFIBERSATTACHTOCELL ATTACHMENTSITESAREWALLPLASMAMEMBR
Broward College - MCB - MCB2010
MULTIPLICATIONOFBACTERIOPHAGESThebasicmechanismforviralmultiplicationissimilarforallviruses.Wehavethebestunderstandingoflifecyclesofthebacteriophages.Onceaphagehasenteredacell,therearetwopossibilitiesforthenextsteps.Theseareknownasthelyticcycleandthels
Broward College - MCB - MCB2010
MULTIPLICATIONOFBACTERIOPHAGESAfterthis,biosynthesisofotherviralproteinsbegins.mRNAistranscribedfromphageDNAsothatanyadditionalphageenzymesandcapsidproteinscanbesynthesized.Thesearecalledlateproteins.Thereisaperiodduringwhichnocompletephageparticlesar
Broward College - MCB - MCB2010
MUTATION: CHANGE IN THE GENETICMATERIALAmutationisachangeinthebasesequenceofDNA.Thiswillsometimescauseachangeintheproductencodedbythatgene.Ifthegeneisanenzyme,theenzymemaybecomemoreorlessactivebecauseofachangeintheaminoacidsequence.Ifthemutationmakest
Broward College - MCB - MCB2010
NEMATODES(THEROUNDWORMS)Thesewormsareroundincrosssectionandtaperedateachend.Theyhaveacompletedigestivesystem,withamouth,anintestine,andananus.Mostaredioecious.Malesaresmallerthanfemales.Manynematodesarefreelivinginsoilandwater,buttherearealsomanyparasi
Broward College - MCB - MCB2010
NONPROTEOBACTERIAGRAMNEGATIVEBACTERIAtheseareonlydistantrelativesofthegramnegativeproteobacteria.1.Cyanobacteriathesehaveabluegreencolorandresemblealgae.Theycarryoutoxygenicphotosynthesisandmanyofthemfixnitrogen.Specialcellscalledheterocystscontainenzy
Broward College - MCB - MCB2010
NonspecificDefensesofHostSincemicrobesarefoundinsuchlargenumbersinourenvironmentandareequippedwithvariousmeansofinvadingandcausingdisease,wemustbeabletodefendourselvesagainstthem.Ourabilitytofightdiseaseiscalledresistance,andoccursinthreemainways:1.Pr
Broward College - MCB - MCB2010
NORMALMICROBIOTA(NORMALFLORA)REGIONNORMALFLORAORGANISMSCOMMENTSSKINPropionibacteriunmacnesSecretionsfromsweatandoilglandshaveantimicrobialproperties,somicrobesindirectcontactmaynotliveforlongperiods,butlargenumbersalwayspresent.Staphylococcusepi
Broward College - MCB - MCB2010
NUCLEARAREA(NUCLEIOD)prokaryoticcellsdonothaveaseparate,membraneenclosedareafortheDNA.TheirDNA,intheformofonecircularchromosomewithoutassociatedhistones,isnotseparatedfromthecytoplasm.ThechromosomesDNAtwistsintoatighthelixandisconnectedtotheplasmamembr
Broward College - MCB - MCB2010
NUTRITIONALADAPTATIONSFungimayadaptwelltoenvironmentsthatdonotsupportgrowthofbacteria.Tocomparethetwogroups:1.FungiusuallypreferapHofabout5,whichistooacidformostbacteria2.Almostallmoldsareaerobic.Mostyeastsarefacultativeanaerobes.3.Mostfungiaremorere
Broward College - MCB - MCB2010
OBSERVINGMICROORGANISMSTHROUGHAMICROSCOPEMagnificationisnecessarytoobserveandstudymicrobes.Also,weknowallmicrobesaresmall,butwemuststillbeabletomeasuretheirsize.Unitsofthemetricsystemareused.Themeteristhestandardunit,butsincethisisroughlyequivalenttoo
Broward College - MCB - MCB2010
OBTAININGPURECULTURESMostsamplesfromnaturalconditionscontainmanydifferentspeciesofbacteria,andoftenothertypesofmicrobesaswell.Eachmicrobeplacedonthesurfaceofasolidmediumgrowsandformsavisiblecolony.Coloniesformedbydifferentmicrobesmayhavedifferentappear
Broward College - MCB - MCB2010
OPERON MODEL OF GENEEXPRESSIONThisisadescriptionofthedetailsofthecontrolofgeneexpressionbyinductionandrepression.InductionoftheenzymesinvolvedinlactosecatabolisminEscherichiacolicanbeusedastheexample.Threeenzymesareneededfortakinginandbreakingdownlac
Broward College - MCB - MCB2010
OPERON MODEL OF GENEEXPRESSIONWhenlactoseispresent,asmallamountofitentersthecellandisconvertedtoallolactose.Allolactoseactsasaninducer.Itbindstotherepressorproteinandaltersitsothatitcannotbindtotheoperatorsite.RNApolymerasecannowtranscribethestructura
Broward College - MCB - MCB2010
OPPORTUNISTIC MICROORGANISMSUndercertaincircumstances,relationshipsbetweenorganismscanchange.Forexample,commonstrainsofEscherichiacoliareharmlessorbeneficialinthelargeintestine.However,E. coliisaverycommoncauseofurinarytractinfectionsifitgainsaccesstot
Broward College - MCB - MCB2010
ORGANICGROWTHFACTORSTheseareessentialorganiccompoundsanorganismcannotsynthesize.Vitaminsareoneexample.Thesefunctionascoenzymesandarerequiredforessentialmetabolicreactions.Manybacteriacansynthesizealltheirownvitamins(humanscanmakeonlyafew),butsomedoreq
Broward College - MCB - MCB2010
OSMOTICPRESSUREPlacingahighconcentrationofsaltorsugarinbacterialsurroundingscauseswatertoleavethecell.Thisisverysimilartoplaindesiccation.Itcausestheplasmamembranetoshrinkawayfromthecellwallplasmolysis.Thecellstopsgrowingandeventuallydies.Thisisawayof
Broward College - MCB - MCB2010
OTHER ANTIMICROBIALSMODEOFACTIONCOMMENTSANTIFUNGALDRUGSPOLYENESAmphotericinBInjurytoplasmamembraneSystemicfungalinfections.Fungicidal.IMIDAZOLES&TRIAZOLESInhibitionofplasmamembranesynthesis.Fungicidal.ClotrimazoleTopicaluseforfungalinfectionso
Broward College - MCB - MCB2010
OXYGENREQUREMENTSOFMICROBES1.Obligateaerobes(aerobicmicrobes)theserequireoxygentoliveandgrow(thisdescriptionalsofitsus!).Theymustproduceenzymesthatcounteractthetoxiceffectsofoxygen.Theadvantageofusingoxygenisthataerobiccellularmetabolismisthemosteffici
Broward College - MCB - MCB2010
PATHOGENICPROPERTIESOFFUNGI,PROTOZOA,HELMINTHS,ANDALGAE1.Fungisomefungicancausedisease,butasagrouptheydonothaveawelldefinedsetofvirulencefactors.a.Somefungidoproducetoxicchemicals.Trichothecenesaretoxinsthatinhibitproteinsynthesisineukaryoticcells.b
Broward College - MCB - MCB2010
PATHOGENIC PROPERTIES OF VIRUSESVirusesmustgainaccesstothehost,evadethehostsdefenses,andcausedamageordeathtothehostcellwhilereproducingthemselves.1.Viralmechanismsforevadinghostdefensesviruseshaveattachmentsitesforreceptorsonhostcells.Thisallowsthevir
Broward College - MCB - MCB2010
PATHOGENIC PROPERTIES OF VIRUSES4)Somevirusesmaycauseadjacentcellstofusetogether,formingaverylargecellcalledasyncitium.Paramyxovirusesareknownforthis.5)Someviralinfectionsresultinchangesinfunctionbutnovisiblechangesinappearance.6)Somevirusinfectedcell
Broward College - MCB - MCB2010
PATHOLOGY, INFECTION, ANDDISEASEPathologyisthescientificstudyofdisease.Itincludes:1.Etiologythecauseofthediseasediseasecausingorganismsarecalledpathogens2.Pathogenesisthemannerinwhichthediseasedevelops3.Stucturalandfunctionalchangesbroughtaboutbythed
Broward College - MCB - MCB2010
PERSISTENTVIRALINFECTIONSThistermreferstoaviralinfectionthatoccursgraduallyoveralongperiodoftime.Thevirusisgraduallybuildingupoveralongperiodoftime,andinmostcaseseventuallywillbefatal.Theseinfectionscanalsobecalledslowviralinfections.Anexampleisthatver
Broward College - MCB - MCB2010
pHMostbacteriagrowbestatapHof6.57.5(neutralornearneutral).MostbacteriadonotgrowatallbelowapHofabout4butafewacidophilesdotolerateacidity.MoldsandyeastspreferapHof56,buttendtogrowatleastsomeoverawiderangeofpH.Acidfoodssuchaspicklesandsauerkrautusuallydo
Broward College - MCB - MCB2010
PHAGOCYTOSISCelleatingcertainwhitebloodcellshavetheabilitytoengulfmicrobesandotherforeignparticles.Thesecellsareknownasphagocytes,andincludeseveraltypesofwhitebloodcells.Areviewofbloodbloodconsistsofafluidcalledplasma,inwhichtheformedelementsarefound.
Broward College - MCB - MCB2010
PHAGOCYTOSISThegranularleukocytesaretheneutrophils,basophilsandeosinophils.Whenstained,theircytoplasmexhibitslargeprominentgranules.1.Neutrophilslilac(pinkish)granuleswithalobednucleus.Theseareoftencalledpolymorphonuclearleukocytes,polymorphs,orpolys.
Broward College - MCB - MCB2010
PHASESOFPHAGEGROWTHa.Latentperiodnumberofplaqueformingunits(viruses)remainsconstant.Phagesareinvadinghostcellsandcausingthesynthesisofviralproteinsandthereplicationofthenucleicacid,butnonowvirionsarebeenreleased.b.Eclipseperiodfirstpartofthelatentper
Broward College - MCB - MCB2010
PHYSICALMETHODSOFCONTROLDryingandsaltingweretheearliestmethodsoffoodpreservation,sothesewerealsotheearliestmeansofcontrollingbacterialgrowth.Physicalmeansofcontrolinclude:HeatOsmoticpressureFiltrationRadiationLowtemperaturesDesiccationWhenchoosinga
Broward College - MCB - MCB2010
PLASMIDS, LYSOGENY, ANDPATHOGENICITYPlasmidsaresmallcircularpiecesofDNAthatarenotpartofthemainbacterialchromosomeandcontaingenesnotfoundonthemainchromosome.Theyarereplicatedandpassedontodaughtercellsduringcelldivision.OnegroupofplasmidsareknownasRplas
Broward College - MCB - MCB2010
PLASMIDSPlasmidsaresmallcircularpiecesofDNAfoundinsomebacteria(butnotall)andoccasionallyineucaryocytessuchasyeasts.Theycarrygeneswhicharenotfoundonthebacterialchromosome.Usuallythecellcansurviveandgrowwithoutthesegenes,butundercertainconditionstheplas
Broward College - MCB - MCB2010
PLATYHELMINTHS(THEFLATWORMS)1.Trematodes(flukes)thesehaveflat,leafshapedbodieswithaventralsuckerandanoralsucker,whichtheyusetoholdthemselvesinplaceandtosuckfluidsfromthehost.Theycanalsoabsorbfoodthroughtheiroutercovering,calledthecuticle.Theircommonnam
Broward College - MCB - MCB2010
PLATYHELMINTHS(THEFLATWORMS)8)Whenahumaneatsthecrayfishthathasnotbeensufficientlycooked,themetacercariaecomeoutofthecystinthehumanssmallintestine.Itboresoutoftheintestineandtravelstothelungs,entersthebronchiolesanddevelopsintoanadult.c.Schistosoma(blo
Broward College - MCB - MCB2010
PORTALS OF ENTRY1.MUCOUSMEMBRANESmanybacteriaandvirusesgainaccesstothebodybypenetratingthemucousmembranesoftherespiratory,digestive,orgenitourinarytracts,orbypenetratingtheconjunctiva(mucousmembranethatlinestheeyelids).Therespiratorytractisthemostfre
Broward College - MCB - MCB2010
PORTALS OF EXITMicrobeshavepreferredportalsofentryandexitfromthebodyofahost.Thischapterismostconcernedwithspreadofdisease,soportalsofexitarediscussed.1.Respiratorytractpathogenslivinginthisareaarecarriedoutbyacoughorasneeze.Thelistincludestuberculosis
Broward College - MCB - MCB2010
PREPARATIONOFSPECIMENSFORLIGHTMICROSCOPYItisnotimpossibletoviewunstainedmicrobeswithbrightfieldmicroscopy.Thisisusuallydonebyoneofthefollowingmethods:TEMPORARYWETMOUNTadropofliquidcontainingthemicrobesisplacedonaslide.Acoverslipisplacedoverthedropandt
Broward College - MCB - MCB2010
PRINCIPLESOFMICROBIALCONTROLTERMS:1.Sterilizationdestructionofallformsofmicrobiallifeincludingendospores,whicharethemostresistantform.Thisismostoftendonewithheat.2.CommercialsterilizationtermusedforcannedfooditmustbeheatedenoughtokillendosporesofClos
Broward College - MCB - MCB2010
PROKARYOTICCELLSSizemostrangefrom0.2to2mindiameter,28minlengthShapeCoccus(sphere),Bacillus(rod),SpiralArrangement:a.Coccimanywillbesingle,butsometimeswhencellsdividetheymayremainattachedtoform:1)Diplococci(pairs)2)Streptococci(chains)3)Tetrads(gro
Broward College - MCB - MCB2010
PROKARYOTICGROUPSBacteriaallhaveincommontheprokaryoticcelltype,buttherearemanygroupsandmanywaysofclassifyingthem.BergeysManualisthemostwidelyacceptedauthority.BacteriamakeuptwoofthethreedomainsthedomainBacteria(thecommoneverydaybacteria)andthedomainAr
Broward College - MCB - MCB2010
PROTEINCATABOLISMIntactproteinscannotcrossbacterialplasmamembrane,sobacteriamustproduceextracellularenzymescalledproteasesandpeptidasesthatbreakdowntheproteinsintoaminoacids,whichcanenterthecell.Manyoftheaminoacidsareusedinbuildingbacterialproteins,but
Broward College - MCB - MCB2010
ProtozoaAmoebic dysenteryEntamoeba histolyticadiarrhea & damage to intestinal wallmay beassociated with produce such as lettuceGiardiasisGiardia lambliaprolonged diarrheal diseaseoften associated with drinkingwater from outdoor sources such as streams
Broward College - MCB - MCB2010
PROTOZOAThisgroupconsistsofunicellular,eukaryoticorganismsbelongingtothekingdomProtista.Theyliveinwaterandsoil,andallarechemoheterotrophs.Thevegetativestageisknownasatrophozoite.Onlyafewofthe20,000speciescausedisease,andsomeareconsideredpartofthenorma
Broward College - MCB - MCB2010
PURINEANDPYRIMIDINEBIOSYNTHESISDNAandRNAconsistoflinkedstrandsofbuildingblockscallednucleotides.Eachnucleotidecontainsanitrogenousbase(apurineorapyrimidine),apentose(5carbon)sugar,andaphosphategroup.PurinesPyrimidinesAdenineCytosineGuanineThymineUr
Broward College - MCB - MCB2010
RADIATION1.Xraysandgammaraysionizeatomsandmolecules.Thiscanbefataltocells,butinlesserdosestheseformsofradiationactasmutagens.Asionsareformed,theycancombinewithbasesinDNA,causingerrorsinDNAreplicationandrepair.Insomecases,chromosomesmayevenbreakapart.
Broward College - MCB - MCB2010
RECOMBINANTDNAANDBIOTECHNOLOGYGeneticengineering(recombinantDNAtechnology)wasfirstcarriedoutin1973.Thefirstproductproducedthisway,humaninsulin,wasputonthemarketin1982.Recombinationisthetransferofnewgenesintoacellbytransformation,conjugation,ortransduc
Broward College - MCB - MCB2010
REGULATION OF BACTERIAL GENEEXPRESSIONCellsmustregulatethesynthesisofproteins,makingonlythoseproteinsthatareneededatanyparticulartime.Manyoftheseproteinsareenzymes,whichcatalyzethechemicalreactionsthatoccurwithinthecell.Byregulatingtheactivityofthegen
Broward College - MCB - MCB2010
RELATIONSHIPBETWEENTHETWOBRANCHESOFTHEIMMUNERESPONSEThetwobranchesoftheimmuneresponsearecloselyinterrelated,inseveralways:1.PRODUCTIONOFANTIBODIEStheproductionofantibodiesagainstmanyantigensrequirestheassistanceofhelperTcells.TheseantigensarecalledTde
Broward College - MCB - MCB2010
RELATIONSHIPS BETWEEN THE NORMAL MICROBIOTA AND THE HOSTNormalfloraorganismsaredesirablebecausetheycanpreventthegrowthoflargenumbersofpathogens.Thisiscalledmicrobialantagonismandismainlyduetocompetitionamongmicrobes.Ifpathogenscomealong,normalfloramaya
Broward College - MCB - MCB2010
RIBOSOMESsomeareattachedtoroughER,othersarefreeincytoplasm.Theirfunctionisproteinsynthesis.Eukaryoticribosomesareslightlydifferentfromthoseofprokaryocytes.Eukaryocyteshave80Sribosomes,withone60Sandone40Ssubunit.Thesubunitsaremadeinthenucleolusandleavet