4: Exercise Endocrine System Physiology: Activity 1: Metabolism and Thyroid Hormone Lab Report Pre-lab Quiz Results You have not completed the Pre-lab Quiz.
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Experiment Results Predict Question: Predict Question 1: Make a prediction about the basal metabolic rate (BMR) of the remaining rats compared with the BMR of the normal rat you just measured. Your answer : b. The hypophysectomized (Hypox) rat's BMR will be higher than the normal rat's BMR. Predict Question 2: What do you think will happen after you inject thyroxine into the three rats? Your answer : a. The normal rat will become hyperthyroidic and develop a goiter. Predict Question 3: What do you think will happen after you inject TSH into the three rats? Your answer : d. The normal rat will become hyperthyroidic and will not develop a goiter. Predict Question 4: Propylthiouracil (PTU) is a drug that inhibits the production of thyroxine by blocking the attachment of iodine to tyrosine residues in the follicle cells of the thyroid gland (iodinated tyrosines are linked together to form thyroxine). What do you think will happen after you inject PTU into the three rats? Your answer : c. The hypophysectomized rat will become hypothyroidic and develop a goiter. Stop & Think Questions: 1h. Calculate the oxygen consumption per hour for this rat using the following equation. ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour Enter the oxygen consumption per hour in the field below and then click Submit to display your results in the grid. You answered: .12 ml O2/hr 1i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms to use this equation). Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr Enter the metabolic rate in the field below and then click Submit to display your results in the grid. You answered: 300 ml O2/kg/hr 2h. Calculate the oxygen consumption per hour for this rat using the following equation. ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour Enter the oxygen consumption per hour in the field below and then click Submit to display your results in the grid. You answered: .103 ml O2/hr 2i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms to use this equation). Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr Enter the metabolic rate in the field below and then click Submit to display your results in the grid.
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You answered: .103 ml O2/kg/hr 3h. Calculate the oxygen consumption per hour for this rat using the following equation. ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour Enter the oxygen consumption per hour in the field below and then click Submit to display your results in the grid. You answered: .105 ml O2/hr 3i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms to use this equation). Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr Enter the metabolic rate in the field below and then click Submit to display your results in the grid. You answered: .105 ml O2/kg/hr Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800). The normal rat is Your answer : c. hyperthyroid Correct answer: b. euthyroid Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800). The thyroidectomized (Tx) rat is Your answer : c. hyperthyroid Correct answer: a. hypothyroid Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800). The hypophysectomized (Hypox) rat is Your answer : c. hyperthyroid Correct answer: a. hypothyroid Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800) after the thyroxine injection. The normal rat is Your answer : a. hypothyroid Correct answer: c. hyperthyroid
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Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800) after the thyroxine injection. The (Tx) thyroidectomized rat is Your answer : b. euthyroid Correct answer: c. hyperthyroid Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800) after the thyroxine injection. The hypophysectomized (Hypox) rat is Your answer : b. euthyroid Correct answer: c. hyperthyroid Which of the rats developed a goiter after injection with thyroxine (select all that apply)? You correctly answered: d. None of the rats developed a goiter with this injection. Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800) after the TSH injection. The normal rat is You correctly answered: c. hyperthyroid Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800) after the TSH injection. The thyroidectomized (Tx) rat is You correctly answered: a. hypothyroid Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800) after the TSH injection. The hypophysectomized (Hypox) rat is Your answer : a. hypothyroid Correct answer: c. hyperthyroid Which of the rats developed a goiter after injection with TSH (select all that apply)? Your answer: b. the thyroidectomized rat Correct answer: a. the normal rat c. the hypophysectomized rat Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800) after the PTU injection.
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The normal rat is You correctly answered: a. hypothyroid Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800) after the PTU injection. The thyroidectomized (Tx) rat is Your answer : c. hyperthyroid Correct answer: a. hypothyroid Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid levels; BMR above 1800) after the PTU injection. The hypophysectomized (Hypox) rat is Your answer : c. hyperthyroid Correct answer: a. hypothyroid Which of the rats developed a goiter after injection with PTU (select all that apply)? You correctly answered: a. the normal rat Experiment Data: Rat Normal Tx Hypox Normal Tx Hypox Normal Tx Hypox Normal Tx Hypox Weight (g) 250 244 246 250 244 246 250 244 246 250 244 246 ml O2/min 7.2 6.2 6.3 8.3 7.9 7.8 8.1 6.3 7.8 6.2 6.4 6.3 ml O2/hr .12 .103 .105 498 474 468 486 378 468 372 384 378 BMR (ml O2/kg/hr) 300 .103 .105 1992 1943 1902 1944 1549 1902 1488 1574 1537 Palpation No mass No mass No mass No mass No mass No mass Mass No mass Mass Mass No mass No mass Injected none none none Thyroxine Thyroxine Thyroxine TSH TSH TSH PTU PTU PTU
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Post-lab Quiz Results You scored 0% by answering 0 out of 7 questions correctly. 1. How would you treat a thyroidectomized animal so that it functions like a "normal" animal? Your answer: a. Provide the animal TRH supplements. Correct answer: c. Provide the animal T4 supplements. 2. As a result of the missing hormone(s) in the hypophysectimized rat, what would be some expected symptoms? You did not answer this question. Correct answer: d. decreased basal metabolic rate 3. An injection of thyroxine to an otherwise normal rat will cause which of the following? You did not answer this question. Correct answer: b. hyperthyroidism 4. Why didn't any of the rats develop a goiter after thyroxine injection? You did not answer this question. Correct answer: a. In all cases, TSH levels were not elevated by the thyroxine injection. 5. Why did the normal rat develop a palpable goiter with the TSH injection? You did not answer this question. Correct answer: c. The TSH receptors on the thyroid gland were excessively stimulated. 6. An injection of propylthiouracil to an otherwise normal animal will cause which of the following? You did not answer this question. Correct answer: b. goiter development 7. Why did the normal rat develop a palpable goiter with the propylthiouracil injection? You did not answer this question. Correct answer: d. The injection decreased the negative feedback mechanism on TSH.
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Review Sheet Results You have not completed the Review Sheet.
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