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Midterm 1 Exam Review-1

Course: BIO 301, Spring 2012
School: Cincinnati
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1 Midterm Exam Review 1. Draw a peptide bond between any two amino acids at the pH of 6 and circle the peptide bond? Also, indicate all alpha carbons? 2. Consider the follow reaction. X Y + D ; Keq = 10^-8. Calculate the standard free-energy change? 3. Consider a reaction similar as in question 3, whos G0 is determined to be +2.9kcal/mol. a. Calculate the equilibrium constant of the reaction? b. In spite of the positive G0, how can this reaction go on inside a cell? 4. Consider the follow reaction GTP GDP + Pi. For this reaction the G0 is 4.9kcal/mol. [GTP]= 10-1mM, [GDP]= 10-3mM, [Pi]=10-5. Calculate G for this reaction? 5. Consider the same reaction as in question 4. If Keq for this reaction was found to be 10-2. Calculate G, using the same concentration as in question 4. 6. Define endergonic and exergonic in terms of Reactions? 7. List the 3 mechanisms by which enzymes accelerate reactions. Lowering activation energy does not count as one. 8. Define the role of heat shock protein or molecular chaperones? 9. Which fatty acid has a higher melting point: cis unsaturated or trans unsaturated bonds? Why? 10. What is the major difference between a phospholipid and a triglyceride? 11. Fe0 + Cu2+ Fe2+ + Cu0 a. Define oxidation, reduction, oxidizing agent, reducing and agent? b. For the reaction indicate what is being oxidized/reduced. What is the oxidizing agent and reducing agent? 12. How many different step of energy utilization are there in Glycolysis and what is common among them? 13. Define catabolic and anabolic? 14. When PFK-1 is inhibited by ATP this is called and why? 15. ATP is ______________ and a ______________ inhibitor for PFK-1? 16. Under what condition does fermentation occur? What form of NAD is used in fermentation? What do yeast and human produce from fermentation? What would be the out come of glycolysis if fermentation did not occur? 17. Define Gluconeogenesis? 18. Define Substrate-level phosphorylation? 19. Draw a Michaelis Manton and Lineweaver-Burk graph. Also included draw the affects of a competitive and noncompetitive inhibitor on the same graphs? Label all axis and define Vmax , Vmax/2 and Km for every line. 20. For the table below fill in the blanks. Graph a Lineweaver-Burk plot of the final results. Find Vmax and Km for both lines and determine which type of inhibitor was used? Table 1: EnzymeA TubeA [S] TubeB TubeC TubeD 8 4 2 0.08 OD/min 6 0.054 0.034 0.02 Table 2: Inhibitor [S] OD/min TubeA TubeB TubeC TubeD 8 6 4 2 0.04 0.025 0.018 0.01

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2/20/2012A reaction can run in a cell in spite of apositive G if1. The substrate isadded to high levels2. The product is takenaway3. Either of the above4. Neither of theaboveGlycolysis requires ATP1. True2. False60%94%20%16%5%he. .. .

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UC Davis - BIOLOGY - BIS104

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UC Davis - BIOLOGY - BIS104

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UC Davis - BIOLOGY - BIS104

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UC Davis - BIOLOGY - BIS104

BIS 104 PQ #2424. The data presented in Figure 5 suggest that clustering of integrins in the PM of the cellstimulates VEGF expression. Remember that clustering in this context means that the integrinproteins must be induced to move laterally in the pla

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UC Davis - BIOLOGY - BIS104

BIS 104 PQ 25In Figure 6, data are presented to support the contention that 53 ligand bindinginduces phosphorylation of Shc as part of an outside-to-inside signaling pathway.Regarding Shc:a. What is it composed of?b. Where is it located in free-float

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UC Davis - BIOLOGY - BIS104

BIS 104 PQ 2626. The data in Figure 6a show results of experiments designed to search for an intracellular(cytoplasmic) mediator for the outside-to-inside signal pathway involving 53 integrins inLNCaP cells. These experiments were designed with the pri

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UC Davis - BIOLOGY - BIS104

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UC Davis - BIOLOGY - BIS104

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UC Davis - BIOLOGY - BIS104

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BIS 104 PQ 30

UC Davis - BIOLOGY - BIS104

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BIS 104 PQ 31

UC Davis - BIOLOGY - BIS104

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BIS 104 PQ 32

UC Davis - BIOLOGY - BIS104

BIS 104 PQ 32The conclusions that can be derived from all of the experimental data presented in Figures 1 6in the S. De et al. article can be summed up in four simple statements:a. The activation state of 35 integrin plays a critical role in tumor grow

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UC Davis - BIOLOGY - BIS104

OBSERVATION: LNCap cells lacking functional 53 integrins growvery slowly and are not stimulated to proliferate when exposed to proteingrowth factors. In contrast, LNCap cells with functional 53 integrinsproliferate more rapidly when exposed to growth f

Assignment 4-Draft 2