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### Midterm 1 Exam Review-1

Course: BIO 301, Spring 2012
School: Cincinnati
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Word Count: 396

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1 Midterm Exam Review 1. Draw a peptide bond between any two amino acids at the pH of 6 and circle the peptide bond? Also, indicate all alpha carbons? 2. Consider the follow reaction. X Y + D ; Keq = 10^-8. Calculate the standard free-energy change? 3. Consider a reaction similar as in question 3, whos G0 is determined to be +2.9kcal/mol. a. Calculate the equilibrium constant of the reaction? b. In spite of...

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1 Midterm Exam Review 1. Draw a peptide bond between any two amino acids at the pH of 6 and circle the peptide bond? Also, indicate all alpha carbons? 2. Consider the follow reaction. X Y + D ; Keq = 10^-8. Calculate the standard free-energy change? 3. Consider a reaction similar as in question 3, whos G0 is determined to be +2.9kcal/mol. a. Calculate the equilibrium constant of the reaction? b. In spite of the positive G0, how can this reaction go on inside a cell? 4. Consider the follow reaction GTP GDP + Pi. For this reaction the G0 is 4.9kcal/mol. [GTP]= 10-1mM, [GDP]= 10-3mM, [Pi]=10-5. Calculate G for this reaction? 5. Consider the same reaction as in question 4. If Keq for this reaction was found to be 10-2. Calculate G, using the same concentration as in question 4. 6. Define endergonic and exergonic in terms of Reactions? 7. List the 3 mechanisms by which enzymes accelerate reactions. Lowering activation energy does not count as one. 8. Define the role of heat shock protein or molecular chaperones? 9. Which fatty acid has a higher melting point: cis unsaturated or trans unsaturated bonds? Why? 10. What is the major difference between a phospholipid and a triglyceride? 11. Fe0 + Cu2+ Fe2+ + Cu0 a. Define oxidation, reduction, oxidizing agent, reducing and agent? b. For the reaction indicate what is being oxidized/reduced. What is the oxidizing agent and reducing agent? 12. How many different step of energy utilization are there in Glycolysis and what is common among them? 13. Define catabolic and anabolic? 14. When PFK-1 is inhibited by ATP this is called and why? 15. ATP is ______________ and a ______________ inhibitor for PFK-1? 16. Under what condition does fermentation occur? What form of NAD is used in fermentation? What do yeast and human produce from fermentation? What would be the out come of glycolysis if fermentation did not occur? 17. Define Gluconeogenesis? 18. Define Substrate-level phosphorylation? 19. Draw a Michaelis Manton and Lineweaver-Burk graph. Also included draw the affects of a competitive and noncompetitive inhibitor on the same graphs? Label all axis and define Vmax , Vmax/2 and Km for every line. 20. For the table below fill in the blanks. Graph a Lineweaver-Burk plot of the final results. Find Vmax and Km for both lines and determine which type of inhibitor was used? Table 1: EnzymeA TubeA [S] TubeB TubeC TubeD 8 4 2 0.08 OD/min 6 0.054 0.034 0.02 Table 2: Inhibitor [S] OD/min TubeA TubeB TubeC TubeD 8 6 4 2 0.04 0.025 0.018 0.01
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UC Davis - BIOLOGY - BIS104
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