reaction 2/20/2012
A can run in a cell in spite of a
positive G if
1. The substrate is
added to high levels
2. The product is taken
away
3. Either of the above
4. Neither of the
above
Glycolysis requires ATP
1. True
2. False
60%
94%
20%
16%
5%
he
.. .
.. .
1. True
2. False
89%
Fa
l
Tr
ue
Ne
i
Oxidation of glucose via glycolysis
requires molecular oxygen
se
of
t
th
e
th
er
of
er
Eit
h
Th
e
Th
e
pr
o
du
c
su
bs
tra
ti
te
s..
.
.. .
6%
Which molecule has less internal
energy?
1. Methane
2. Carbon dioxide
60%
40%
If the cell has a lot of ATP, the first step
of glycolysis is shut down.
1. Yes
2. No
87%
e
ox
id
di
bo
n
Ca
r
se
Fa
l
Tr
ue
M
et
ha
ne
11%
At high ATP concentrations,
phosphofructokinase is
1. actively producing
fructose 1,6bisphosphate
2. not producing
fructose 1,6bisphosphate
85%
15%
..
in
g.
pr
od
uc
no
t
ac
t iv
el
No
Ye
s
yp
ro
du
. ..
13%
1
2/20/2012
Which site of phosphofructokinase has
higher affinity for ATP?
1. Catalytic site
2. Regulatory site
61%
Which is the oxidized form?
1. NAD+
2. NADH
92%
39%
The process of fermentation results in
Reduction of NAD+
Reduction of NADH
Oxidation of NAD+
Oxidation of NADH
67%
10%
In the enzyme phosphofructokinase,
the regulatory ATP site has a higher Km
than the ATP catalytic site
99%
rti
a
Te
Se
co
nd
a
ry
ry
of
N.
..
da
t
io
n
da
t
Ox
i
33%
8%
io
n
of
N.
..
...
of
N
n
tio
Re
du
c
tio
n
of
N
...
8%
Hydrolysis of the phosphate from PEP
provides sufficient energy to make ATP
from ADP 100%
1. True
2. False
0%
se
se
Fa
l
Tr
ue
1%
Fa
l
1. True
2. False
Tr
ue
Re
du
c
1. Primary
2. Secondary
3. Tertiary
Pr
im
ar
y
15%
Which aspect of protein structure
would be most significantly affected by
phosphorylation
58%
Ox
i
1.
2.
3.
4.
NA
DH
NA
D+
Re
gu
l
Ca
t
aly
at
or
tic
ys
it .
..
sit
e
8%
2
2/20/2012
The same enzymes are used for
gylcolysis and gluconeogenesis in all
but three steps.
99%
In a FRAP experiment, the following results were seen.
The two samples represented cells with different
amounts of cholesterol, both at 37C. Which sample
had more cholesterol?
85%
1. True
2. False
Which of the following is not
amphipathic?
e#
pl
in
in
e
in
e
1. They are high in cholesterol
2. They are high in sphingolipids
3. They are wider than the standard
membrane
4. They are detergent insoluble
3%
5. All of the above are true
Ar
g
61%
22%
11%
e
ar
tru
e
re
he
ab
ov
ea
ns
ol
. ..
er
ge
n
ti
an
w
e
ar
de
t
id
er
th
sp
h
in
h
Th
ey
of
t
go
l..
.
th
e.
..
2%
in
es
te
ch
ol
in
h
hi
g
hi
g
e
re
ar
Th
ey
se
Fa
l
m
Gl
ut
a
Which if the following is not true of
lipid rafts
ro
l
15%
3%
Al
l
85%
Tr
ue
1. True
2. False
8%
Th
ey
ip
id
nt
go
l
id
ge
Sp
hi
n
De
te
r
id
e
er
Ph
os
ph
ol
ip
lyc
Tr
ig
At 37C cholesterol reduces membrane
fluidity
17%
c in
e
9%
3%
Lysine
Leucine
Glutamine
Arginine
Le
u
16%
1.
2.
3.
4.
e
72%
Ly
sin
Triglyceride
Phospholipid
Detergent
Sphingolipid
Integral proteins have transmembrane helices
which interact with the phosphilipids. Which of
the following amino acids is likely to be in such
helices
72%
Th
ey
a
1.
2.
3.
4.
Sa
m
Sa
m
Fa
l
Tr
ue
se
pl
e#
1
1%
2
15%
1. Sample #1
2. Sample #2
3
2/20/2012
Integral proteins may cross the bilayer
multiple times
1. True
2. False
Interactions between membrane proteins and
networks can often be disrupted by changing salt
concentration. What is most likely being distupted
here?
97%
99%
1.
2.
3.
4.
Covalent linkages
Ionic linkages
Hydrogen bonding
Hydrophobic
interactions
1%
1%
1%
Which of the following can influence protein
mobility in a membrane?
95%
Temperature
Cytoskeleton
Extracellular matrix
All of the above
in
.. .
.
Hy
dr
op
ho
bi
c
bo
nd
i..
n
og
e
...
ly
c
ive
ne
ga
t
Fa
l
se
3%
Tr
ue
se
A
iv
po
sit
A
97%
8%
Fa
l
Hy
dr
ol
e
lm
ut
ra
ne
A
Water enters the cell if the cell is
placed in a hypotonic solution
1. True
2. False
92%
Tr
ue
el
yc
. ..
...
ab
ov
e
m
at
r ix
ll
o
A hypotonic solution has a lower
solute concentration than the cell
1. True
2. False
23%
12%
ft
he
le
to
n
la
r
ke
llu
yt
os
1%
0%
tr
ac
e
1. A neutral molecule
2. A positively charged
ion
3. A negatively
charged ion
Ex
C
Te
m
pe
ra
tu
re
4%
If the [internal]/[external] is the same,
the G for __________ will be the
most negative
65%
A
1.
2.
3.
4.
Io
ni
c
Co
va
l
Fa
ls
e
Tr
ue
en
tl
lin
ka
g
in
ka
...
es
2%
4
2/20/2012
Looking at an enzyme activity, we see
a Vmax because
1. Substrate is limiting
2. Enzyme is limiting
(turnover number)
87%
The internal concentration of glucose
in a red blood cell is always lower than
the blood concentration (hint: red
blood cells have the glycolytic
enzymes)
1. True
2. False
13%
0%
10
se
Fa
l
Tr
ue
En
zy
m
e
Su
bs
tra
is
te
is
lim
l..
.
i. .
.
0%
Countdown
A scientist makes a preparation of red blood cell
ghosts resealed with 1mM glucose inside. These are
put into a solution of 1mM glucose. Which way will
the glucose go?
1. From outside in
2. From inside out
18%
t
ou
ls
e
Tr
ue
Fr
om
in
s
10
Fa
id
e
id
e
ou
ts
Fr
om
82%
1. True
2. False
0%
in
0%
Given the same concentration gradient, an
anion would have a more negative DG than a
neutral molecule
Countdown
All eukaryotic cells have aquaporins
1. True
2. False
93%
When an acetylcholine receptor opens
1. Sodium ions rush
into the cell
2. Sodium ions rush
out of the cell
72%
28%
ru
...
ns
io
m
So
di
u
m
So
di
u
ls
e
Fa
Tr
ue
io
ns
ru
...
7%
5
2/20/2012
When a voltage gated potassium
channel opens
1. Potassium ions rush
into the cell
2. Potassium ions rush
out of the cell
88%
The internal concentration of glucose
in a red blood cell is always lower than
the blood concentration (hint: red
69%
blood cells have the glycolytic
enzymes)
31%
1. True
2. False
ns
...
io
The immediate source of energy for
glucose transport into intestinal cells is
ATP
78%
1. True
2. False
18%
ou
t
in
22%
Blocking ATP production can block
glucose uptake into intestinal cells
se
Fa
l
Fr
om
Tr
ue
in
s
id
e
id
e
ou
ts
Fr
om
Fa
l
Tr
ue
A scientist makes a preparation of red blood cell
ghosts ( no glycolytic enzymes left) resealed with
1mM glucose inside. These are put into a solution of
82%
1mM glucose. Which way will the glucose go?
1. From outside in
2. From inside out
se
siu
m
Po
ta
s
Po
ta
s
siu
m
io
ns
...
12%
In the Na,K ATPase cycle, the protein is
open ___________ as a result of
:10
phosphorylation.
50%
51%
50%
1. To the inside
2. To the outside
1. True
2. False
th
e
To
To
se
Fa
l
Tr
ue
th
e
in
s
ou
ts
id
e
id
e
49%
6
2/20/2012
Inhibiting ATP synthesis directly inhibits
glucose uptake from the intestinal lumen10
50%
1. True
2. False
50%
Inhibiting ATP production eventually blocks
glucose uptake from the lumenal side of 10
50%
50%
the intestine
If the internal concentration were to
go down from 140mM to 50 mM
(external stays the same), what would
50%
50%
happen to the membrane potential?
Fa
ls
e
Tr
ue
Fa
ls
e
Tr
ue
1. False
The True
2. resting membrane potential is
closer to
1. Sodium equilibrium potential
2. Potassium equilibrium potential
50%
50%
.
siu
m
eq
ui
m
Po
ta
s
50%
ch
an
siu
m
Po
ta
s
0 of
5
m
ch
a
nn
el
...
50%
So
di
u
Po
ta
s
siu
m
ch
an
nn
el
ch
a
m
10
:10
The acetylcholine receptor is a ligand
activated _______
1. Sodium channel
2. Potassium channel
50%
...
50%
So
di
u
0 of
5
So
di
u
:10
The acetylcholine receptor is a ligand
activated _______
1. Sodium channel
2. Potassium channel
eq
ui
..
lib
.. .
be
co
m
. ..
ou
ld
It
w
It
w
ou
ld
be
co
m
. ..
1. It would become
more negative
2. It would become
less negative
10
7
2/20/2012
Inhibition of the sodium-potassium
ATPase results in
1. The potassium
equilibrium
potential
2. The sodium
equilibrium
potential
82%
eq
u.
..
m
...
of
e
In
1. Oxidized
2. Reduced
86%
14%
ed
Re
du
c
d
di
ze
Ox
i
Tr
an
sp
or
t
er
m
e.
..
iff
us
i..
.
ed
m
em
br
an
ito
ch
M
NAD+ is _________ to produce NADH
6%
pl
so
di
u
yt
op
la
s
94%
Sim
lm
at
rix
8%
C
...
Pr
io
rh
yp
er
po
l. .
Pr
.
io
rd
ep
ola
r i.
Tw
..
o
of
th
e
ab
o.
..
What is the most likely means of
getting pyruvate into a
mitochondrion?
1. Simple diffusion
2. Transporter
mediated process
20%
ne
r
1%
0%
71%
1. Cytoplasm
2. Mitochondrial
matrix
3. Inner membrane of
the mitochondrion
m
5%
Where in a eukaryotic cell are the
glycolytic enzymes located?
fv
ol
ag
. ..
olt
ng
o
ng
v
ni
Op
en
i
Op
e
68%
Th
e
Th
e
De
cr
e
po
t
as
ed
as
siu
Ca
+
m
+.
..
...
++
Ca
se
d
ea
In
cr
The depolarization phase of the action
potential is based on
1. Opening voltage gated sodium
channels
2. Opening of voltage gated
potassium channels
3. Prior hyperpolarization due to 26%
AchReceptors
4. Prior depolarization based on
AchReceptors
5. Two of the above
5%
. ..
18%
95%
on
dr
ia
1. Increased Ca++ in
cardiac cells
2. Decreased Ca++ in
cardiac cells
During an action potential, the
membrane potential approaches
8
2/20/2012
During glycolysis, when NAD+ is
converted to NADH, NAD+ is acting as
Which side has the lower pH?
...
ge
ga
ng
in
izi
re
du
c
ox
id
0 of
5
10
A
Th
e
in
te
rm
10
Countdown
In the process of glucose oxidation,
CO2 is produced in the cytoplasm
1. True
2. False
50%
a.
..
50%
br
...
em
m
Th
e
0 of
5
1. An oxidizing agent
2. A reducing agent
50%
at
r ix
50%
An
1. The matrix
2. The intermembrane
space
Countdown
Conversion of pyruvate to acetyl CoA
releases CO2
1. True
2. False
77%
99%
23%
During the Krebs cycle
CO2 is produced
NAD+ is produced
H2O is produced
Acetyl CoA is
produced
5. Two of the above
are correct
se
Fa
l
Tr
ue
Fa
l
Tr
ue
se
1%
Which side has the lower pH?
1.
2.
3.
4.
81%
1. The matrix
2. The intermembrane
space
73%
27%
15%
2%
rm
em
br
...
at
r ix
in
te
Th
e
m
Th
e
is
pr
od
uc
e.
..
Ac
et
yl
Co
A
is
Tw
.. .
o
of
th
e
ab
o.
..
1%
.
H2
O
od
uc
..
pr
is
is
CO
2
NA
D+
pr
od
u
ce
...
1%
9
2/20/2012
During glycolysis, when NAD+ is
converted to NADH, NAD+ is acting as
1. An oxidizing agent
2. A reducing agent
Which is the strongest reducing agent?
68%
80%
16%
11%
2
DH
DH
ge
N
FA
A
A
N
...
a.
..
A
An
re
du
c
ox
id
in
izi
ga
ng
D
5%
D+
20%
NAD+
NADH
FAD
FADH2
FA
1.
2.
3.
4.
Which is the strongest oxidizing agent?
The more negative the redox potential, the
stronger the reducing agent
65%
1. True
2. False
Pyruvate
Lactate
Oxygen
Water
21%
16%
11%
r
ls
e
n.
..
11%
M
ot
e
m
ic
a
he
em
br
an
e
lp
po
te
...
e
te
rm
em
br
an
In
at
rix
38%
89%
1. Membrane potential
aspect of PMF
2. Chemical potential
aspect of PMF
C
1. Matrix
2. Intermembrane
space
Using a chloride ionophore disrupts the
n.
..
62%
Fa
W
As a result of electron transport, one side
of the membrane has a pH of 7 and the
other 8. Which side is pH 8?
M
Tr
ue
at
e
ge
n
O
La
ru
v
Py
xy
ct
at
e
2%
at
e
1.
2.
3.
4.
84%
10
2/20/2012
13%
.
a.
NADH
FADH2
Pyruvate
Two of the above
All of the above
An
sw
er
s1
an
d
. ..
NA
D+
43%
7%
1%
id
e
0%
in
s
id
e
60%
1. O
2. L
3. T
e
he
of
t
Al
l
of
t
o
Tw
In what conformation of the ATP synthase
is ATP actually made from ADP and Pi?
ab
ov
e
at
e
ab
ov
Py
ru
v
he
FA
DH
2
NA
DH
pH
pH
8
7
on
on
th
e
th
e
in
s
an
d
s1
er
An
sw
Th
e
Th
e
H.
.
,p
49%
1.
2.
3.
4.
5.
35%
. ..
5%
2%
Py
ru
v
H+
c
br
a
em
m
24%
An appropriate substrate for
submitochondrial particles in this
experiment would be
Submitochondrial particles will make ATP if
65%
an artificial pH gradient is established with
1. pH 8 on the inside, pH
7 on the outside
2. pH 7 on the inside and
pH 8 on the outside
Pyruvate
NADH
NAD+
Answers 1 and 2
Answers 1 and 3
on
ce
ne
nt
.. .
p.
..
24%
1.
2.
3.
4.
5.
at
e
1. The membrane potential
generated by electron
transport
2. The H+ concentration
gradient generated by
electron transport
Which of the following would work as
a substrate for this experiment
(isolated vesicles)
56%
NA
DH
Pi uptake by occurs in the presence of
a Cl- ionophore. That means that Pi
uptake depends on
76%
Moving electrons from H2O to NADP+ is a
thermodynamically spontaneous process
86%
1. True
2. False
24%
ls
e
Fa
Tr
ue
T
14%
L
O
16%
11
2/20/2012
What would we see if RDG tripeptide
is added to the experiment?
1. Cells would lyse
2. Cells would adhere
normally
3. Cells would not
adhere
93%
Adherens junctions are like _____
because both connect to actin
1. hemidesmosomes
2. focal adhesions
77%
23%
4%
re
os
om
he
m
id
fo
ca
la
es
m
ls
Ce
l
w
ls
Ce
l
Malate Dehydrogenase will be active
in a broken mitochondrial preparation
1. True
2. False
dh
es
io
ns
es
no
ta
ou
ld
w
ou
ld
Ce
l
ad
ls
he
r
w
e
ou
ld
no
r
dh
e
...
ly
se
3%
The tripeptide RGD plays a key role in
binding fibronectin to integrin. This
peptide is part of the sequence of
75%
71%
1. Integrin
2. Fibronectin
25%
ne
c
Fib
ro
In
te
se
Fa
l
th
e
o
of
es
ab
ov
es
e
1%
Tw
ju
n
ct
io
ct
io
ns
ns
s
ct
io
n
1%
A
dh
Ti
er
e
ns
gh
t
ju
n
ab
o.
..
he
Al
l
of
t
o
Tw
of
t
he
bu
l
glo
m
un
o
Im
ab
o.
..
in
. ..
in
s
s
tin
he
r
Se
le
c
Ca
d
1%
1%
D
5%
ju
n
6%
97%
ap
27%
Tight junctions
Adherens junctions
Gap junctions
Desomsomes
Two of the above
G
1.
2.
3.
4.
5.
58%
6%
Which of the following junctions use
cadherins
om
so
m
Tr
ue
Which of the following attachments is
heterophilic?
1. Selectins
2. Cadherins
3. Immunoglobulin
super family
4. Two of the above
5. All of the above
t in
gr
in
29%
12
2/20/2012
Which of the following interactions involve
actin on the intracellular side of the
membrane?
26% 27%
Desomsomes
Hemidesmosomes
Adherens Junctions
Focal adhesions
Two of the above
23%
16%
1. True
2. False
7%
e
s
io
n
ab
ov
es
th
e
of
Fa
l
se
o
Tw
Tr
ue
ct
io
ns
la
dh
ca
Fo
ns
Ju
n
m
os
er
e
dh
A
es
D
em
id
es
om
so
m
es
om
es
14%
H
1.
2.
3.
4.
5.
Once a ribosome has made a protein
through the membrane-bound route,
it will continue to make only 86%
membrane bound proteins
13