Lecture 1/31/12 3: The Nucleus
The nuclear membrane
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The nuclear pore complex (NPC)
EM
From the Nuclear side
Nuclear Transport (Gated transport) [Dr. Kwaks part]
2
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Lamina Assembly
Intermediate
proteins
progeria
control
The nuclear lamina
3
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Nucleolus
Function of Nucleolus: transcribe and assemble rRNA into
ribosome subunits
Chromosomes
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The cell cycle
The Chromosomes during Cell Cycle
Three major components of an eukaryo6c chromosome
kinetochore
5
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Chromosome Packing
The SMCs in Condensins
Chromosome Packing
The Nucleosome
6
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The Histone Code (epigene6cs)
Decipher the Histone
The Codes packaging informa6on can be inherited
7
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The Centromere
A simple model for centromere in yeast
Karyotype: a complete set of chromosomes at mitosis
The human genome comprises 23 pairs of chromosomes.
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Experiments in my lab
Cell line 155
46,XX,t(2;22)
The banding paBerns of human chromosomes
(stained with Giemsa stain)
8466
46,XX (normal)
Real exp
9
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Abnormalities
Ch 4
Ch 12
Ch 4
Ch 12
Recap
Structure of Envelope and nuclear pore
complex
Function of the Nucleolus
Nuclear lamina
Chromosome packaging: nucleosomes, 30 nm
fibers.
Nucleosome structure and components
Histone code
Karyotype analysis
10