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Ill. Chicago - PSCH - 270
Mood Disorders and SuicideAbnormal Psychology Robin Rosenberg and Stephen KosslynPowerPoint Slides prepared by: Nicholas Greco IV, M.S. College of Lake County1In any particular year, about 8% of Americans experience a mood disorder. The DSMIVTR distin
Ill. Chicago - PSCH - 270
Abnormal Psychology Robin Rosenberg and Stephen Kosslyn PowerPoint Slides prepared by: Nicholas Greco IV, M.S. College of Lake CountyAnxiety Disorders1Anxiety is a sense of agitation or nervousness, which is often focused on an upcoming possible dange
Ill. Chicago - PSCH - 270
Dissociative and Somatoform DisordersAbnormal Psychology Robin Rosenberg and Stephen KosslynPowerPoint Slides prepared by: Nicholas Greco IV, M.S. College of Lake County1Read the textbook's discussion of Anna O. and her treatment. There's more to this
Ill. Chicago - PSCH - 270
Substance Use DisordersAbnormal Psychology Robin Rosenberg and Stephen KosslynPowerPoint Slides prepared by: Nicholas Greco IV, M.S. College of Lake County1 A _ substance is a chemical that alters mental ability, mood, or behavior. Substance use diso
Ill. Chicago - PSCH - 270
Eating DisordersAbnormal Psychology Robin Rosenberg and Stephen KosslynPowerPoint Slides prepared by: Nicholas Greco IV, M.S. College of Lake County1Anorexia nervosa is an eating disorder characterized by being at least 15% below expected body weight
Ill. Chicago - PSCH - 270
Gender and Sexual DisordersAbnormal Psychology Robin Rosenberg and Stephen KosslynPowerPoint Slides prepared by: Nicholas Greco IV, M.S. College of Lake County1Gender identity is the _ of being male or female, as these categories are defined by the pe
Ill. Chicago - PSCH - 270
Schizophrenia and Other Psychotic DisordersAbnormal Psychology Robin Rosenberg and Stephen KosslynPowerPoint Slides prepared by: Nicholas Greco IV, M.S. College of Lake County1Schizophrenia is characterized by psychotic symptoms that significantly aff
Ill. Chicago - PSCH - 270
Personality DisordersAbnormal Psychology Robin Rosenberg and Stephen KosslynPowerPoint Slides prepared by: Nicholas Greco IV, M.S. College of Lake County1Personality defines the individual's enduring ways of thinking, feeling, and behaving; and the tr
Ill. Chicago - PSCH - 270
Childhood DisordersAbnormal Psychology Robin Rosenberg and Stephen KosslynPowerPoint Slides prepared by: Nicholas Greco IV, M.S. College of Lake County1Mental Retardation is intelligence that is significantly below normal an IQ approximately equal to
Ill. Chicago - PSCH - 270
Abnormal Psychology Robin Rosenberg and Stephen Kosslyn PowerPoint Slides prepared by: Nicholas Greco IV, M.S. College of Lake CountyCognitive Disorders1Cognitive disorders are psychological disorders in which the primary symptom is _ mental abilities
University of Texas - BIO - 366
Figure 1.1A-form DNA 11 bp/turn Most RNA and RNA-DNA duplex exist in this form Shorter, wider helix than B form Deep, narrow major groove not easily accessible toproteinsWide, shallow minor groove (but lower informationcontent than major groove)F
University of Texas - BIO - 366
Box 1.2Figure 1.11Figure 1.123 copies of Pol III, 2 on thelagging strand, are thought tomake synthesis more efficientThe trombone model for DNA replication usestwo DNAPs, one for leading, the other forlagging strand synthesis. The 2 enzymes arebo
University of Texas - BIO - 366
Figure 1.21Figure 1.18Whathappenswhenreplicationforksmeet?Incircularbacterialchromosomes,asingleterminationregionslowsthetworeplicativehelicases(directionally).Thepositivesupercoilsthataregeneratedaheadofthetheforkareremovedbytopoisomerases(DNAgyrase
University of Texas - BIO - 366
Figure 2.3Figure 2.11Figure 2.6Figure 2.12Different sigma factors recognize different -35 and -10 sequences-35-10rpoD (70K)TTGACATATAATrpoE (24K)GAACtttgTCaAarpoH (32K) TNtCNCcCTTGAArpoS (38K) CTTGACAAArpoF (28K) TAAAgtttfecI (19K)CCCCATt
University of Texas - BIO - 366
Figure 2.19Figure 2.38Figure 2.20Primarily RNase III and RNase EFigure 2.24Figure 2.21Figure 2.25Figure 2.29Codons UCC/UCU both interact wellwith the same anticodon on tRNAThe first base of the anticodon can make wobble base-pairs with the third
University of Texas - BIO - 366
Figure 2.3230S subunitFigure 2.27Table 2.2EF-TsEf-Tu recyclingTable 2.1The (almost) universal genetic codeUGA also codes for selenocysteine (but thetranslational apparatus requires more thanjust the UGA triplet for incorporation)All codons used
University of Texas - BIO - 366
1. (6 points) One oriT, whose sequence is unique, is recognized by one relaxase in order to transfer the entireplasmid to another bacterium by conjugation.The other two oriT sequences have essentially the same core sequence and are recognized by a disti
University of Texas - BIO - 366
BIO 3661.Exam ISpring 2012(4 points) Guanosine 5-triphosphate 3-diphosphate2.(4 points) The C-terminal domain of the subunit is essential for high levels of transcription of theribosomal (and some other) operons. The C-terminal domain directly cont
University of Texas - BIO - 366
1.(6 points) GroE (GroEL+GroES) is a member of the Hsp60/Hsp10 family. GroEL is a dimer of aheptameric cylinder and GroES acts a heptameric cap. Fig. 2.39 describes how it works2.(6 points) ClpP (with ClpA or ClpX): cytoplasmic, non-essentialLon (La)
University of Texas - BIO - 366
1.Infect gal+ cells with +, isolate lysogen. Induce and grow to lysis. Lysate will contain a few dgalparticles due to aberrant excision that included gal as part of but lost the tail gene J. This a LTF lysateConcentrate the phages in the lysate and inf
University of Texas - BIO - 366
Figure 2.3230S subunitFigure 2.27Table 2.2EF-TsEf-Tu recyclingTable 2.1The (almost) universal genetic codeUGA also codes for selenocysteine (but thetranslational apparatus requires more thanjust the UGA triplet for incorporation)All codons used
University of Texas - BIO - 366
Box 2.5 FigureBox 2.5 TableFolding Proteins - chaperoninsFigure 2.39groE = (groEL + groES) consute one chaperone system for folding proteins correctlyA second system is (DnaK + DnaJ) - named after their genetic discovery asproteins important in corr
University of Texas - BIO - 366
Figure 2.44Figure 2.45Box 2.7BFigure 3.1Phenotype: BioGenotype: bioPhenotype: HisGenotype: hisAs we learn more, this mutant maybe called: hisG, and later: hisG4The wild-type is His+, and unless emphasis or clarity is necessary,e.g., during geneti
University of Texas - BIO - 366
The Newcombe experimentFigure 3.6Also read about the Lederberg experiment, where replica plating was inventedFigure 3.9Many common mutagens cause predominately transitionse.g., nitrosoguanidine, nitrosourea, MMS, EMS and other alkylating agents;hydr
University of Texas - BIO - 366
EXAM 1Suppression of nonsense codonsFigure 3.20Table 3.4Figure 3.21Selecting RevertantsReplica PlatingFigure 3.22Reciprocal recombination yields equal numbers of each typeFigure 3.23Complementation (no recombination allowed)Figure 3.24Failure
University of Texas - BIO - 366
Crosses involving selected and unselected markersFigure 3.26ConjugationF plasmid-containing cellF+ (male)Both cells become maleSee Figure 5.1 for more detailsNo F plasmidF- (female)F+ x F- -> 2 x F+Fx F- -> 2 x FHfr x F- -> Hfr + F- (usually)F
University of Texas - BIO - 366
2 cross-overs between a circular chromosome and alinear DNA are required to obtain a viable recombinantFigure 3.39A single cross-over between two circular DNAscan result in viability of the co-integrateInactivating genes by recombination with a plasm
University of Texas - BIO - 366
Replication mechanism of ColE1 repliconsFigure 4.8All replicons (incl. chromosomes) require at least one origin-specificpositive regulator of replication and at least one negative regulatorFigure 4.9Replication of plasmid R1Figure 4.10Replication a
University of Texas - BIO - 366
Cloning by inactivation of a selectable markerFigure 4.21Cloning into pUC by blue/white selectionFigure 4.22GTAlacZ reading frame (bottom strand; top strand sequence is shown)Initiationcodon. AGGA .-5S-D sequencePromoter-Cloniing large DNA mol
University of Texas - BIO - 366
Figure 5.6Figure 5.2F+ -> HfrFigure 5.14Occasionally, the transposable element will cause integration of the F plasmid into nonhomologous regions of another DNA molecule (e.g., the chromosome or another plasmid)Hfr -> FFigure 5.15Selecting for F pl
University of Texas - BIO - 366
Mating in the EnterococciFigure 5.18Recipient makes pheromones(small peptides released from the signalsequences of normal cellular lipoproteins)Donor contains tra functions, butonly TraC, a surface protein issynthesized at high levelsPheromone rec
University of Texas - BIO - 366
Figure 7.1Figure 7.8A collection of PodoviridaeCapsidgp10A+BInternal coregp14, gp15, gp16Headproteingp6.7Connectorgp8Tail proteingp7.3Major tailgp11, gp12Tail fibergp17capsidtailfiberfiberLife cycle of phage T4Figure 7.2
University of Texas - BIO - 366
Exam 2Average=57.6Median=59Exam 1+2Median=120.5Life cycle of phage T4Figure 7.2Figure 7.8http:/bilbo.bio.purdue.edu/~viruswww/Rossmann_home/movies/movies.phphttp:/www.mansfield.ohio-state.edu/~sabedon/bgnws020.htm#submissionsFigure 7.3All trans
University of Texas - BIO - 366
Degradation of a bacterial capsule by phageT7 Phage displayBox 7.2Figure 7.8Figure 7.14Fusions to the non-essential Hocand Soc (both referred to as headdecoration proteins are used forT4 display phagesFusions to the coat protein(gpVIII) or the a
University of Texas - BIO - 366
The phage developmental cycle is synchronousFigure 7.2A600TimeS107S105Figure 7.20Control of lysisRecombination with phage: infect a permissive cell with a high multiplicity of two parentsFigure 7.21Complementation: infect a non-permissive cell w
University of Texas - BIO - 366
Figure 8.7Plaques of phage are turbid, especially in their centerThe light source is underneath darker spots mean more light is coming throughThe two life-cyclesFigure 8.1The linear genetic map of Figure 8.2PAQThe circular genetic map of Figure 8
University of Texas - BIO - 366
Figure 8.4cII O PcII O PFigure 8.5gpN binds to boxBE. coliTGCTCTTTAACAABound gpN recruits the Nus proteins, which can then bind to the mutantboxA, interacting with RNAP to directtranscription antiterminationAntitermination by gpN allows maximal t
University of Texas - BIO - 366
Figure 8.13Figure 8.14Induction of to form a transducing lysateThis is specialized transduction can only acquire bio or galPart of the + genome must be lost in the transducing particle:pbio phages are viable and make plaques they only lost non-essent
University of Texas - BIO - 366
Figure 8.18Figure 8.19Figure 9.1Figure 9.2Figure 9.3Figure 9.4Figure 9.5Figure 9.6Figure 9.7Figure 9.8
University of Texas - BIO - 366
Figure 9.8Transposition is usually a rare event the Tn 3 resolvase represses transcription of tnpA, the transposaseFigure 9.9DonorNon-mobilizable plasmidRecipientFigure 9.10Non-mobilizable plasmidConjugative plasmid (not chromosome)Figure 9.11Re
University of Texas - BIO - 366
Transposon mutagenesisFigure 9.21Figure 9.26Using phage Mu, as a transposon (Mud(lac), to make gene fusionsMu ends (inverted repeats)Figure 9.27A deletion variant of Mu: mini-Mu, can be used for in vivo cloning and transductionFigure 9.28Infection
University of Texas - BIO - 366
Exam 3Average=51.4Median=54.5Exam 1+2+3 Homologous RecombinationE. coli:Eukaryotic homologous recombination:Several factors required in E. coli havehomologs in humans and othereukaryotes:E. coli recombinase RecA = Rad51 ineukaryotes (plus Rad51
University of Texas - BIO - 366
RecombineeringBox 10.3ssDNA does notrequire exo or gamExo degrades from the 5->3direction, producing a 3-ssDNAtail on the PCR product then binds the ssDNA tailand catalyzes strandinvasion just like RecAFigure 11.1Figure 11.2Figure 11.38-oxoG
University of Texas - BIO - 366
Figure 11.14Removal of bulky lesions in DNA by UvrABCRecombinational repair after the replisome encountersa lesion on the leading strand template strandFigure 11.15Another model invokes fork regression to repair leading strand template damageFigure
University of Texas - BIO - 366
Figure 11.20Figure 11.21Error-prone DNA polymerases (DNAP IV and V)[also DNAP II]Table 11.2Table 11.3HTH (helix-turn-helix is a common sequence-specific DNA-binding motifBox 12.1Complementation in the lac operonFigure 12.1Figure 12.2Figure 12.3
University of Texas - BIO - 366
There are 3 operator-like sequences in the lac operonFigure 12.5The importance of the pseudo-operatorsFigure 12.6lacUV5 AMoving the lac operon close to tonB (T1rec)Construct lac [F (Ts replicon) lac+]Select Lac+ at high temperature frequency ~10-4
University of Texas - BIO - 366
Figure 12.18AraC can only activate transcription in the presence of arabinoseFigure 12.19Figure 12.20Figure 12.1590% of the regulation of the trp operon is by the TrpR repressorFigure 12.17A subtle change in TrpR structure in thepresence of Trp al
University of Texas - BIO - 366
Exam 4Average = 63Median = 66Exam 1 - 4Table 13.1Figure 13.1Box 13.1B. subtilis regulator CcpA (Catabolite control protein A)Is the counterpart of CAP-cAMP regulationFigure 13.2Different modes of activation by CAP-cAMPFigure 13.3lac one of the
University of Texas - BIO - 366
All DNA synthesis goes in the 5-> 3 directionpppN53OHNpppChain growth in 3-> 5direction?No 3-deoxynucleoside triphosphates ever found!3pppN5Deoxynucleoside 3-triphosphates5OH53Chain growth in 5-> 3direction533Deoxynucleoside 5-triphosp
University of Texas - BIO 347 - 347
-1Bio 347, 11-22-11Exam 3 AKEYNote: Please enter your name, EID, mark the box for exam A or B, and record your answerson the scantron provided. We will not give credit for answers marked on the exam that differfrom the scantron-we will grade only t
University of Texas - BIO 347 - 347
-1Bio 347, 11-22-11Exam 3 B KeyNote: Please enter your name, EID, mark the box for exam A or B, and record your answerson the scantron provided. We will not give credit for answers marked on the exam that differfrom the scantron-we will grade only t
University of Texas - BIO 347 - 347
1Bio 347, 9-22-11Exam 1 ANote: Please place your name, EID, mark the box for exam A or B, and place youranswers on the scantron provided. We will not give credit for answers marked on the examthat differ from the scantron-we will grade only the scant
University of Texas - BIO 347 - 347
1Bio 347, 9-22-11Exam 1 BNote: Please place your name, EID, mark the box for exam A or B, and place youranswers on the scantron provided. We will not give credit for answers marked on the examthat differ from the scantron-we will grade only the scant
University of Texas - BIO 347 - 347
-1Bio 347, 10-25-11Exam 2 ANote: Please enter your name, EID, mark the box for exam A or B, and record your answers onthe scantron provided. We will not give credit for answers marked on the exam that differ from thescantron-we will grade only the sca
University of Texas - BIO 347 - 347
-1Bio 347, 10-25-11Exam 2 B KEYNote: Please enter your name, EID, mark the box for exam A or B, and record your answers onthe scantron provided. We will not give credit for answers marked on the exam that differ from thescantron-we will grade only the
University of Texas - BIO - 320
LAST NAME:_FIRST NAME:_Exam #1BIO 320Dr. ChanThere are 35 multiple choice questions worth 2 points each.Use a #2 pencil to answer the multiple-choice questions.WRITE YOUR LAST NAME ON THE SCANTRON FORMThere are 4 essay questions worth a total of 3
University of Texas - BIO - 320
LAST NAME:_FIRST NAME:_Exam #1Bio 320Dr. ChanThere are 34 multiple choice questions worth 2 points each.Use a #2 pencil to answer the multiple choice questions.WRITE YOUR LAST NAME ON THE SCANTRON FORMThere are 4 essay questions worth a total of 3
University of Texas - BIO - 320
LAST NAME:_FIRST NAME:_Exam #2BIO 320Dr. ChanThere are 35 multiple choice questions worth 2 points each.Use a #2 pencil to answer the multiple-choice questions.WRITE YOUR LAST NAME ON THE SCANTRON FORMThere are 5 essay questions worth a total of 3
University of Texas - BIO - 320
!"#$%!&#'()*!!!+,-$%!&#'()*!!!!!!"#$%&'%()*%'+,%-./%01#2%!!%./0/!10/!23!4567896/!:.;8:/!<5/=78;>=!?;07.!@!9;8>7=!/1:.A!B=/!1!C@!9/>:86!7;!1>=?/0!7./!4567896/D:.;8:/!<5/=78;>=A!E-,%(!FGB-!"#$%!&#'(!G&!%H(!$I#&%-G&!+G-'!!!%./0/!10/!3!/=1J
UC Irvine - CHEM 1B - 44060
Gas Law StoichiometryQuestion:Calculatethevolume(L)ofCO2gasat26.0Cwithapressureof754torrproducedfroma4.31gmixturethatis37.2%CaCO3.TheCaCO3isthesolesourceofCO2thatisproducedaccordingtothebalancedequation.CaCO3(s)CO2(g)+CaO(s)Solution:Astheproblemwille
Mannheim Business School - FINANCE - 471
Personal Finance: Another PerspectivePersonal Finance:Another Perspective1Objectives A. Understand the importance of perspective B. Understand our perspective for this course C. Understand the principles upon which thatperspective is based D. Und