HernandezMendezA_W5_A1
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HernandezMendezA_W5_A1

Course: BIOLOGY 1020, Spring 2012

School: North South University

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Anette Hernandez-Mendez Review Sheet Week 5 Exercise 12.1 and 12.2 Questions 1. How are microorganisms destroyed by moist heat? By dry heat? Moist heat coagulates microbial proteins inactivating them. Dry heat does not due much as far as breaking down the proteins; however, if the heat is increased to 160-170 degrees Celsius for extended period of time (1-2 hours) 2. Are some microorganisms more resistant to heat...

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Hernandez-Mendez Review Anette Sheet Week 5 Exercise 12.1 and 12.2 Questions 1. How are microorganisms destroyed by moist heat? By dry heat? Moist heat coagulates microbial proteins inactivating them. Dry heat does not due much as far as breaking down the proteins; however, if the heat is increased to 160-170 degrees Celsius for extended period of time (1-2 hours) 2. Are some microorganisms more resistant to heat than others? Why? Some microorganisms are more resistant to heat than others because each bacteria has different time where they can damage the bacterial protein causing death. Resistance to heat varies with type of proteins and the damaging reaction, no two bacterias are made up of the same enzymes and proteins; all of this information relates to where the bacteria is primarily found and what structures they have to be able to survive their environments. 3. Is moist heat more effective than dry heat? Why? Moist heat is more effective than dry heat because moist heat coagulates microbial protein structures at a faster rate and inactivates the proteins permanently. Some protein structures may be stable with dry heat, with said, dry heat has to be applied at higher temperatures for longer period of time than moist heat in order for microorganism to be inactivated. 4. Why does dry heat require higher temperatures for longer time periods to sterilize than does moist heat? Dry heat requires higher temperatures for longer time periods to sterilize than does moist heat because most proteins can be stable in high heat and are harder to break down. 5. What is the relationship of time to temperature in heat sterilization? Explain. The higher the temperature the less amount of time needed for sterilization and vice versa. 6. Would you recommend boiling or baking to sterilize a soiled surgical instrument? Why? I would recommend boiling the instrument to sterilize because boiling would be considered moist heat. Moist heat will inactivate and coagulate microbial proteins. 7. What kinds of clean hospital materials would you sterilize by baking? Why? Some of the things that I would sterilize with baking in a hospital would be blankets sheets those types of materials. 8. Name some hospital materials that can be sterilized by flaming without harming them? Some hospital materials that can be sterilized by flaming without harming there are any surgical instruments. Exercise 13 Questions 1. Define the principles of sterilization with an autoclave and with a dry-heat oven. Autoclave is a machine that uses pressurized steam to sterilize laboratory material. In the autoclave bacteria is place under high pressurized team that is heated higher than boiling point making this an ideal environment for bacterial death. Dry heat oven uses high temperature heat to sterilized material; this mode of sterilization does not use pressure or water through steam to sterilize. The object is to heat the bacteria at very high temperatures and removing water by dry superheated air. 2. What pressure, temperature, time and are used in routine autoclaving? The pressure, temperature and time use to autoclaving are 15-20 lb under pressure, 121 to 125C, and 15-45 minutes, respectively. 3. What factors determine the time period necessary for steam-pressure sterilization? Dry-heat oven sterilization? The factors used to determine the time period necessary for steam-pressure sterilization are temperature and time. In steam-pressure is the factors of intensity of steam temperature that sterilizes (pressure only provides the means of creating this intensity), when it is given time measured according to the nature of the load in the chamber. In the dry-heat oven, the temperature of the hot air also sterilizes, but only after enough time has been allowed to heat the oven load and oxidize vital components of microorganisms without damaging materials. 4. Why is it necessary to use bacteriologic controls to monitor heat sterilization techniques? It is necessary to use bacteriologic controls to monitor heat sterilization because it is essential to ensure that sterilization is being achieved with each run of the steam-pressure sterilizer. 5. When running an endospore control of autoclaheat. ving technique, why is one endospore preparation incubated without heating? One endospore preparation is incubated without heating to prove that the samples were viable to begin with. 6. Would a culture of E.coli make a good bacteriologic control of heat sterilization techniques? Why? E.coli would not make good controls for heat sterilization techniques because they are not very resistant to moist or dry Page 1 of 2 From Laboratory Manual & Workbook in Microbiology Applications to Patient Care ( 9th ed.). By Josephine A. Morello, Helen Eckel Mizer, and Paul A. Granato Copyright 2006 The McGrawHill Companies, Inc. Reprinted with permission of The McGraw-Hill Companies, Inc. BIO2071_Microbiology Laboratory 7. What characteristics of B. stearothermophilus make it valuable for use as a control organism for heat-sterilization techniques? Explain. 8. What factors determine the choice of a paper strip containing bacterial endospores or a sealed ampule containing an endospore suspension for testing heat-sterilization equipment? 9. Would you choose a dry-heat oven, an autoclave, or incineration to heat sterilize the following items? State why. Soiled dressings from a surgical wound: ______________________________ Surgical instruments: __________________________________________ Clean laboratory glassware: ____________________________________ Clean reusable syringes: ________________________________________ 10. Why should the results of endospore control tests be known before heat sterilized materials are used for patient care? Page 2 of 2 From Laboratory Manual & Workbook in Microbiology Applications to Patient Care ( 9th ed.). By Josephine A. Morello, Helen Eckel Mizer, and Paul A. Granato Copyright 2006 The McGrawHill Companies, Inc. Reprinted with permission of The McGraw-Hill Companies, Inc.

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North South University - BIOLOGY - 1020
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My name is Anette Hernandez-Mendez and I reside in Tampa,Fl. I am attending SouthUniversity Tampa Campus as Health Science major pre-nursing. Currently, I work as a CareCoordinator for Aetna coordinating care for the Medicaid population in several count
North South University - BIOLOGY - 1020
Prokaryotic cells do not have a not have a nucleus because it does not need a nucleus to functionlike a eukaryotic cell. Within the prokaryotic cell there are no membrane-bound organelles, mostreactions happen openly within the cytoplasm. While the nucl
North South University - BIOLOGY - 1020
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