lab 9 Mitosis-1
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lab 9 Mitosis-1

Course Number: BIOLOGY 119, Spring 2012

College/University: Ocean County College

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Mitosis By:HendyZelishovsky Bio119DLScience&theHumanBody Experiment#9 3/17/2012,10:30PM Abstract Thepurposeofthisexperimentwastolearnaboutthephasesofmitosisand cometounderstandhowitisusedinthegrowthinplantsandanimalsandtolearnhow mitoticindexesareusedinmedicalresearch.Thisresultsofthisexperimentwerejust that:knowledgeintheareaofmitosisisnowunderstoodasaresultofthisexperiment. Purpose/Hypothesis...

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cometounderstandhowitisusedinthegrowthinplantsandanimalsandtolearnhow Mitosis By:HendyZelishovsky Bio119DLScience&theHumanBody Experiment#9 3/17/2012,10:30PM Abstract Thepurposeofthisexperimentwastolearnaboutthephasesofmitosisand mitoticindexesareusedinmedicalresearch.Thisresultsofthisexperimentwerejust that:knowledgeintheareaofmitosisisnowunderstoodasaresultofthisexperiment. Purpose/Hypothesis Asstatedbefore,thepurposeofthisexperimentwastolearnaboutthephasesof mitosisandcometounderstandhowitisusedinthegrowthinplantsandanimalsand tolearnhowmitoticindexesareusedinmedicalresearch.Throughstudyingand viewingslidesonthedifferentphasesofmitosis,thisgoalwasachieved. Procedures Thefirststepofthisexperimentwastothoroughlystudyalltheinformationabout mitosisgiveninthemanual.Oncethatwasdone,sixslides,showninthemanual,as well,showingdifferentphasesofmitosiswereviewed.Thequestionsundereachslide wasanswered,andthenfurtherquestionsfromLabPaqonthetopicofmitosiswere answered. Data/Observations Questions A. What is the purpose of mitosis? The purpose of mitosis is so that the body can build tissue. It is used for growth and repair by biological organisms. B. Why is the cell cycle important for organisms? The cell cycle is important for organisms because it makes new cells and replaces old ones. C. What role does mitosis play in the growth of an embryo? Since an embryo grows at a rapid rate and is in constant need of new cells, it uses mitosis. D. What is one main difference in the mitotic processes between plant and animal cells? The main difference of mitotic processes between plant and animal cells occurs during cytokinesis: when the cytoplasm of the cell divides. By animals, a cleavage furrow is formed and then pinches off for new cells- something which does not happen with plant cells. With plants, the dividing membrane is also called a cell plate. E. If the cell that has undergone mitosis originally had the diploid (2n) number of chromosomes, then what number of chromosomes will the nuclei in the two new cells have? The number of chromosomes that the nuclei in the two new cells will be 2n. F. Can mitosis ever be used for reproduction? Mitosis can be used for reproduction. G. What parts of plants are most impacted by mitosis? The root of a plant needs mitosis. H. Why might mitosis be easier to study in the tip of the root? That location grows rapidly, meaning there is a lot of mitosis there. I. Where else in a plant might you find a large amount of mitotic cell division occurring? The root cap might be a good place to find mitosis. J. How might the index mitotic be applied in agriculture? Since mitotic index are used to see the impact of environmental factors on plants, this can be applied in agriculture. K. What did you compute to be the mitotic index of the combined six slides (assume there are 54 total cells)? In comparison to the indexes reflected in Figure 8, from approximately which area of a root was the sample taken? A) 8 / 54 x 100 =14.81 B) Approximately 0.2 mm from root cap L. Answer the following questions about the photos below: NOTE: The questions are located below each image.Mitosis Worksheet Onion root tip photos at 600X and 1000X taken by Stan Carpenter of Hands-On Labs, Inc. 1. What phase is this cell displaying? Anaphase 2. What happens after this phase? Telophase 3. The two center side-by-side cells are displaying two different phases of mitosis. i. What are these phases? Prophase and metaphase ii. What diagnostic features can help you with your decision? There are no two distinctly separate cells forming yet. 4. What phase is this cell displaying? Anaphase 5. How can you tell? The spindle fibers are breaking apart. 6. This cell has clearly visible chromatids. i. What's the next phase this cell will undergo? Telophase ii. Where will the chromatids go in the coming phase? They will be grouped into nuclei. 7. The cell in the lower center is exhibiting signs of what phase of mitosis? Prophase 8. What phase does it appear the cell above is undergoing? Metaphase 9. Explain what happens during anaphase. The sister chromatids separate. 10. When the chromatids separate, what is each called? Chromosome. Results/Analysis Throughthoroughlystudyingtheinformationgivenonmitosisandthrough studyingtheslidesgivenofmitosis,thetopicisnowunderstood.Itisnowknownhow mitosisisusedinthegrowthofplantsandanimalsandalsohowmitoticindexesare usedinmedicalresearch. Conclusions Thisexperimentwassuccessfulinaccomplishingitsgoal.Theconceptofmitosis isnowunderstood.Itisknownthattherearefourstagestomitosis.First,isprophase: whenchromosomesarecondensedinthenucleusandtheenvelopearoundthenucleus disintegrates.Mitoticspindlesstarttoformattheendsofthecellandcentromeresand centriolesorganize.Next,ismetaphase:thereplicatedchromosomesgotowardsthe middleofthecell.Afterthat,isanaphase:whenthespindlefibersgetshorterandthe sisterchromatidsbegintobreakapart.Thecentromeresbreakandthiscreatestwo separateandidenticalchromosomes.Lastly,istelophase:whenthechromosomes elongateandgroupintonucleienvelopedinanuclearenvelope.Allthisisfollowedby cytokinesis:thecytoplasmsdividedandanewcellwallormembraneismade.

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Ocean County College - BIOLOGY - 119
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Ocean County College - BIOLOGY - 119
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