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- Title: Cell Bio Paper Quiz Answers
- Type: Exam
- School: UCSD
- Course: BICD 100
- Term: Winter
Coursehero >> California >> UCSD >> BICD 100
Path: Missouri State >> AAS >> 100 Spring, 2008
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Path: St. Cloud >> ETS >> 157 Spring, 2008
Path: St. Cloud >> ETS >> 157 Spring, 2008
Path: St. Cloud >> ETS >> 157 Spring, 2008
Path: St. Cloud >> ETS >> 157 Spring, 2008
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Path: UMass Dartmouth >> ENL >> 101 Fall, 2007
Path: UMass Dartmouth >> ENL >> 101 Fall, 2007
Path: UMass Dartmouth >> ENL >> 101 Fall, 2007
Path: UMass Dartmouth >> ENL >> 101 Fall, 2007
Path: UMass Dartmouth >> ENL >> 101 Fall, 2007
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Path: Texas >> GOV >> 310L Spring, 2008
Path: UCSB >> CLASSICS >> 40 Fall, 2007
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Path: Wake Forest >> BIO >> 213 Fall, 2007
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Path: Towson >> COMM >> 101 Spring, 2008
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Path: Conn College >> ECON >> 112 Spring, 2008
Path: UIllinois >> ARCH >> 412 Spring, 2007
Path: Towson >> COMM >> 101 Spring, 2008
Path: Missouri State >> CIS >> 100 Spring, 2008
Path: UC Riverside >> PSYCHOLOGY >> 002 Spring, 2008
Path: UC Riverside >> PSYCHOLOGY >> 002 Spring, 2008
Path: Rider >> SOC >> 101 Fall, 2007
Path: Conn College >> ECON >> 112 Spring, 2008
Path: Rider >> SOC >> 101 Fall, 2007
Path: Towson >> COMM >> 101 Spring, 2008
Path: Conn College >> ECON >> 112 Spring, 2008
Path: Conn College >> ECON >> 112 Spring, 2008
Path: Conn College >> ECON >> 112 Spring, 2008
Path: Conn College >> ECON >> 112 Spring, 2008
Path: Rider >> SOC >> 101 Fall, 2007
Path: Rider >> SOC >> 101 Fall, 2007
Path: Wake Forest >> PHY >> 110 Fall, 2007
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Path: Seton Hall >> POLS >> 2105 Spring, 2008
Path: University of Toronto >> PSYCH >> 100 Fall, 2008
Path: University of Toronto >> PSYCH >> 100 Fall, 2008
Path: University of Toronto >> PSYCH >> 100 Fall, 2008
Path: University of Toronto >> PSYCH >> 100 Fall, 2008
Path: University of Toronto >> PSYCH >> 100 Fall, 2008
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110 BICD Paper Quiz 11/16/2007 Dr. Ramlah Nehring First Name: ________________KEY___________ Last Name: ______________________________ Student ID #: _____________________________ Instructions: -This quiz contains 14 questions and one bonus question which will total 60 points on 3 pages plus this cover page -Leave ALL of your personal items including cell phones (off please!) at the front of the classroom -Please put your name on EVERY page of the quiz -Use permanent blue or black pen ONLY on the quiz -Please DO NOT USE pencil or white-out -Please try to answer each problem concisely in the space provided, please have a TA initial your exam if you need to write on the back of any pages -Be prepared to show your student ID when turning in the quiz Page # Score Grader Initials 2 24 points _________________ 3 16 points _________________ 4 20 points _________________ Bonus 2 points _________________ Total: 60 points _________________ 1 First Name: _______________________ Last Name: _______________________ 1. What was the main question (main hypothesis) that the authors were asking in this paper? (6 points) 2 parts: Is the morphology of the Golgi the same in SYF cells (3pts) +1 pt for morphology/structure, +1 pt for Golgi, +1 for SYF Is Golgi transport effected by the Src gene? (3pts) +1Golgi, +1transport, +1Src 2. What was the conclusion of the authors from the results presented in this paper? (6 points) 2 parts: Golgi morphology is changed in SYF cells (3pts) Retrograde transport is effected because the KDEL-R is mislocalized when Src levels are changed (3pts) or Src is not part of anterograde transport (+2 points, partial credit) 3. The cell line SYF is an abbreviation of what three genes that are disrupted in the background of this cell line? (2 points each) Gene 1: Src Gene 2: Yes Gene 3: Fyn 4. What TERM is used for this type of mutation/gene disruption (hint: SYF cells have this in 3 genes)? (2 points) Knockout (+2) Deletion (+1) 5. In regards to the main subject of the paper, the Src protein: A) What kind of protein is Src (the name of its protein family)? (2 points) Tyrosine (+1) Kinase (+1) B) What is the biochemical function that this family of proteins carries out in the cell? (2 points) Phosphorylates 2 First Name: _______________________ Last Name: _______________________ 6. The following will questions be about Figure 1 of the paper which is reproduced here: A) The abbreviations P and C in Figure 1, panels 1C and 1F stand for what? (2 points each) P is an abbreviation for: Perimeter C is an abbreviation for: Compactness B) What was the main question that the authors were trying to ask by performing the experiments shown in Figure 1 of the paper (hypothesis)? (4 points) Is Golgi morphology effected by the deletion/ Knockout of the Src gene? C) What were the authors able to conclude after performing the experiments shown in Figure 1? (4 points) Golgi morphology is disrupted -More compact -Less cisternae -Bloated cisternae 7. What kind of a model organism was used to generate the SYF cell line? (2 points) Mouse (mouse embryonic fibroblast cells) 8. In the conclusions the authors mention that the Src protein is not present in the unicellular model organism, ______YEAST___ which is why they carry out their experiments in mammalian SYF cells. (2 points) 3 First Name: _______________________ Last Name: _______________________ 9. Regarding Figure 2: What kind of microscopy was used to generate the images represented in Figure 2, panels A and B shown here on the right? (4 points) Electron microscopy (or Transmission electron microscopy) 10. What role does the KDEL- Receptor (KDEL-R) play in the cell? (2 points) KDEL-R transports ER lumenal proteins from the Golgi to the ER Transports ER lumenal (+1) Golgi to ER (+1) 11. A) What kind of protein must the Pseudomonas exotoxin be to be transported via the KDEL receptor? (2 points) A resident ER lumenal protein B) What sequence must the Pseudomonas exotoxin have to be transported via the KDEL receptor? (2 points) KDEL or KDEL like 12. What type of intracellular vesicular transport is affected by the addition of the protein Src? (4 points) Retrograde 13. What does the drug Brefeldin A do to the organelles of the cell? (2 points) Fuses the Gogli with the ER 14. What relationship did the authors establish for the protein Src and the location of the KDELReceptor? (2 points each) More Src causes: mislocalization of KDEL-R or slow retrograde transport (partial credit moves into the ER +1) Less Src causes: proper KDEL-R localization (movement into the Golgi +2) Bonus: Name one of the two authors who carried out this work at UCSD (2 points) Frederique Bard or Vivek Malhotra 4
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