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6/14/11 Abstract The objective of this experiment was to isolate different fractions of spinach components using column chromatography and analyze the different fractions using Thin Layer Chromatography and obtain Rf values. The different pigments present in the spinach sample were Chlorophyll b, Xanthophyll, and Carotenes. The chlorophyll b had an Rf value of .44 and .06, xanthophyll had an Rf value of .38, and the carotenes had an Rf value of .71. Introduction The objective of this experiment was to isolate different fractions of spinach components using column chromatography and analyze the different fractions using Thin Layer Chromatography and obtain Rf values. Column chromatography is an efficient way to separate the pigments because by sending solvents with different polarities through the column, the pigment attracted to the solvents polarity will be extracted and taken out of the column. The hexanes will be used to extract the least polar pigment because it is very non polar. The mix of 75/25 hexane/acetone will extract the slightly polar because it is non polar.... View Full Document

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