| Terms |
Definitions |
|
BACTERIAL GENETICS
- in Transposition, what is "jumping"
|
segments of DNA
|
|
BACTERIAL GENETICS
- TransFormation requires
|
- Naked DNA in the environment
(ability to take up DNA from environment is known as "competence")
|
|
BACTERIAL GENETICS
- F+ x F- conjugation is the ONLY way to transfer...
|
- Multiple Antibiotic resistance
|
|
BACTERIAL GENETICS
- what kind of phage infects the bacterium in Specialized Transduction?
|
- Lysogenic phage
|
|
BACTERIAL GENETICS
- in Hfr x F-, what type of genes are transferred?
- what type of genes are NOT transferred?
|
Plasmid AND Chromosomal
- none
|
|
BACTERIAL GENETICS
- in Generalized Transduction, what happens to the bacterial DNA?
- what happens to the viral DNA?
|
- Cleavage of bacterial DNA
- Synthesis of Viral proteins
|
|
BACTERIAL GENETICS
- TransFormation requires what?
- TransDuction requires what?
- Conjugation requires what?
|
(NBC)
- Naked DNA
- Bacteriophage
- Conjugation
|
|
BACTERIAL GENETICS
- in a typical Conjugation (F+ x F-), what type of Antibiotic resistance can occur?
- in a Hfr conjugation (F- x Hfr), then what type of Antibiotic resistance can occur?
|
- ONLY way to transfer MULTIPLE ABx Resistance
- transfer of Single ABx resistance
|
|
BACTERIAL GENETICS
- In F+ x F- Conjugation, the plasmid DNA is replicated in which bacteria?
- once replicated, it will travel via what?
|
F+
F Pilus (Cytoplasmic Bridge)
|
|
BACTERIAL GENETICS
- Methicilin resistance by Staph. aureus was obtained via what process?
|
- TransDuction
(via a Phage)
|
|
BACTERIAL GENETICS
- what is an Hfr cell?
- specify the F-type
|
- when Plasmid is incorporated in to bacterial chromosome
- when F+ plasmid is incorporated into bacterial chromosomal DNA
|
|
BACTERIAL GENETICS
- Transduction requires
|
- Bacteriophage
|
|
BACTERIAL GENETICS
- in Generalized Transduction, how does the bacterial DNA get transferred?
|
- some of the chopped up Chromosomal bacterial DNA may become packaged in viral capsid
(when phage infects another bacterium, genes will be transferred)
|
|
BACTERIAL GENETICS
- the process of TransFormation allows for what type of ABx resistance transfer?
|
- Single ABx Resistance Transfer
|
|
BACTERIAL GENETICS
- TransFormation is a feature especially of what bugs?
|
(SHiN)
- Strep. pneumoniae
- HiB
- Neisseria
|
|
BACTERIAL GENETICS
- in Hfr x F-, the replication of the incorporated plasmid DNA may include?
|
- incorporated Flanking Chromosomal DNA
|
|
BACTERIAL GENETICS
- what kind of phage infects the bacterium in Generalized Transduction?
|
- Lytic phage
|
|
BACTERIAL GENETICS
- under what circumstance can an integrated prophage become induced into a lytic phage replication?
|
- UV light
(is just one example)
|
|
BACTERIAL GENETICS
- in F+ x F- Conjugation, what type of DNA is transferred?
- what type of DNA is NOT transferred?
|
- Plasmid
- Chromosomal DNA
|
|
BACTERIAL GENETICS
- in Specialized Transduction, how does the bacterial DNA get transferred?
|
- when viral DNA is excised, the flanking bacterial genes may be excised with it.
|
|
BACTERIAL GENETICS
- in Hfr x F-, the replication and transfer comes FROM?
|
- Hfr
|
|
BACTERIAL GENETICS
- in TransFormation, what DNA is allowed for uptake?
|
- Any DNA
|
|
BACTERIAL GENETICS
- what is lysogenic conversion?
|
- Lysogeny's ability of converting a NON-Pathogen into a Pathogen (or just increase virulence)
|
|
BACTERIAL GENETICS
- in Transposition, what type of genes are being tranferred?
|
- Plasmid genes to Chromosomes
or
VISE VERSA
|
|
BACTERIAL GENETICS
- in the Conjugation of F+ x F-, which plasmid contains genes required for conjugation?
- bacteria without this gene is termed what?
|
F+ (male b/c of erection)
F- (female)
|
|
BACTERIAL GENETICS
- in Conjugation involving the High Frequency of Recombination (Hfr) cell, what combo allows for recombination?
- what combo allows for DNA transfer?
|
F+ x Hfr
F- x Hfr
|
|
BACTERIAL GENETICS
- list the 3 major ways bacteria diversify their genetics
|
- TransFormation
- TransDuction
- Conjugation
|
|
BACTERIAL GENETICS
- in Specialized Transduction, what happens to the bacterial DNA?
- what happens to the viral DNA?
|
- Viral DNA is incorporated into bacterial chromosome
|
|
BACTERIAL GENETICS
- Most of the Bad Toxins are transferred via what process?
|
- Specialized Transduction
(lysogenic transduction)
|
|
BACTERIAL GENETICS
- what are the pathways that occur for Transduction?
|
- Generalized Transduction
("packaging" event)
- Specialized Transduction
("excision" event)
|
|
BACTERIAL GENETICS
- In Specialized Transduction, what is the DNA called once it integrates into the bacterial DNA?
|
- Prophage
|
|
BACTERIAL GENETICS
- give an example of TransFormation
|
- Rough (R) & Smooth (S) strains of Strep. pneumoniae in the Griffith experiment of 1928
|
|
BACTERIAL GENETICS
- in which genetic process does Chromosomal Recombination occur?
|
- Conjugation btw Hfr & F-
|
|
BACTERIAL GENETICS
- list 5 toxin genes that were transferred via a LYSOGENIC Phage
|
(ABCDE)
- shiga A-like Toxin
- Botulism Toxins (some)
- Cholera Toxin)
- Diphtheriae Toxin
- Erythrogenic Toxin from S.
Pyogenes
|
