Documents about Bacillus Subtilis

  • 1 Pages

    Trp attenuation Du 2000 discussion questions

    Cal Poly Pomona, BIO 560

    Excerpt: ... Bio560 Literature Discussion Questions 5/3/01 Du et al., 2000. trp RNA-binding attenuation protein-5 stem-loop RNA interaction is required for proper transcription attenuation control fo the Bacillus subtilis trpEDCFBA operon. J Bacteriol. 182:1819. 1. What are the differences between the trp operons of Bacillus subtilis and E. coli? Please including the discussion on the regulatory RNA leader region and how Trp level regulates each operon. 2. How did the authors quantify the binding of TRAP to the RNA leader region? 3. In order to map the 5 stem-loop structure, the authors did primer extension experiments using RNA treated with RNAse T1, RNase V1, DMS or CMCT. What is the function of each enzyme/chemical? How can they help solving RNA secondary structure? 4. From this study, does TRAP bind to the 5 stem-loop? What is the role of (G/U)AG repeats in the binding of TRAP to the 5 stem-loop? Lin, Spring 2001 ...

  • 13 Pages

    1. intro handout

    Yale, MCDB 120

    Excerpt: ... taokaetal.,Cell40,19(1985) Key Concept: Evolutionary conservation Nature keeps reusing the same building blocks while constructing an enormous diversity of organisms Researchers exploit these similarities; information gained by studying one organism can lead to better understanding of a similar process in another organism. Key concept: Evolution provides the foundation for modern biology Millions of years Populations evolve to display new biological properties Three Domains of Life (based on DNA comparisons) Over billions of years, gradual changes in the DNA sequence of organisms have resulted in an enormous variety of different species Using DNA sequence comparison these are more closely related than these. Bacillus subtilis E. coli 3 Domain view of life Plants, animals and fungi have only recently diverged from one another. Archaea form a separate grouping. They group more closely to eukaryotes than bacteria do. Key concept: All life is constructed ...

  • 1 Pages

    dicotmous key

    CofC, BIO 310

    Excerpt: ... Dichotomous Key for Identifying Unknown Bacterium #68 Bacillus subtilis Staphyloccus aureus Escherichia coli Unknown#68 Clostridium sporogenes Citrobacter freundii Proteus vulgaris Enterococcus faecalis Enterobacter aerogenes Pseudomonas aeruginosa Gram-Positive Bacillus subtilis Clostridium sporogenes Enterococcus faecalis Staphyloccus aureus Gram-Negative Citrobacter freundii Enterobacter aerogenes Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Unknown#68 Sulfur Reduction Proteus vulgaris Citrobacter freundii Unknown#68 Non-Sulfur Reduction Escherichia coli Enterobacter aerogenes Pseudomonas aeruginosa Lactose Fermentation Citrobacter freundii Unknown#68 Non-Lactose Fermentation Proteus vulgaris ...

  • 3 Pages

    Bio560 final 2001

    Cal Poly Pomona, BIO 560

    Excerpt: ... Bio560 Microbial Physiology Final Exam June 4, 2001 June 4, 2001 Answers are due by Thursday 1 pm (6/7/01); you may turn in a hard copy to my office or e-mail your answer to me. Final grades will be posted by Tuesday, June 12; you may pick up your report paper at the same time. Good luck and have a great summer! Please answer the following questions using the space provided; Please focus the answers covered by the lecture note; 100 points total. 1. The initiation of DNA replication starts the _ period of bacterial cell cycle. (5 points). What is the positive and negative regulator in initiating DNA replication at OriC? Explain briefly how they work together to control the initiation of DNA replication. (15 points) 2. Sporulation in Bacillus subtilis involves temporal and compartmental control at different stages of development. Please list the key molecules/factors that are responsible for the temporal regulation and list their sequence of occurrence and their compartmentalization during the developm ...

  • 2 Pages

    Quiz 3 questions

    Fairfield, BI 151

    Excerpt: ... Lab 3 1. How are microscope slides handled if you do not keep them to review? Microscope slides that you do not wish to keep should be placed in the red, sharps biohaxard container located at the front of the laboratory. 2. For each of the following, list the shape of the bacteria and the grouping of cells that you would expect to see in a stained slide from a broth culture of: Bacillus subtilis , Streptococcus mutans. In a stained slide from a broth culture, the shape of Bacillus subtilis is rod and Streptococcus mutans is a long, fragile chain. 3. What is the difference in one of the first steps in the preparation of a slide of organisms made from a culture growing on nutrient agar as compared to a culture growing in nutrient broth? The difference in one of the first steps in the preparation of a slide of organisms made from a culture growing in nutrient agar as compared to a culture growing in nutrient broth is the use of water on the slide with organisms made from a culture growing on nutrient agar. When u ...

  • 1 Pages


    Brandeis, BIOL 18a

    Excerpt: ... Genetic mapping of critical proteins in tryptophan, tyrosine, and histodine pathways for Bacillus Subtilis , the SB100 strain Bacterial transformation is a well documented phenomenon which allows for exchange of genetic material between cells and their environment, this relationship can be used to determine the recombination frequency for genes and thus a chromosomal position. B. Subtilius undergoes these transformations, and contains critical metabolic pathways that are prone to knockout in prepared strains. Here I studied the uptake of genetic material and frequency of recombination to establish a genetic map for 3 genes, encoding critical proteins in the pathways for tryptophan, histidine or tyrosine. Wild type DNA was isolated, extracted and purified from wild type B. Subtilius and added to SB100 auxotroph (a trp- tyr- his- strain) allowing for transformation. The transformed B. Subtilius were then plated on selective media, lacking between 0 and all 3 of the required amino acids; colonies that grew were t ...

  • 10 Pages

    Lecture #24

    Cornell, BIO 2900

    Excerpt: ... as a REPRESSOR Spo0AP as an ACTIVATOR 8 10/25/2007 Alternative Sigma Factors in Bacillus subtilis . About half are required for sporulation. Others have diverse or unknown functions. K Alternative Sigma Factors involved in sporulation H sigF F early forespore genes early mother cell genes A sigE sigG E sigK K late mother cell genes A F sigE sigG G late forespore genes Stage II E F Stage IV K G 9 10/25/2007 Summary Protein synthesis and activity can be constitutive or can be regulated. Regulation of protein synthesis is often carried out at the level of transcription initiation. Transcriptional regulatory proteins can act as repressors or as activators of transcription initiation. Both classes can be made active or made inactive by small molecules. Examples to understand: Lac Repressor, CAP, ArgR, MalT. Variations: LexA a repressor that is inactivated by protein cleavage. p p y g LuxR/LuxI induction in response to pheromones made by sibling ba ...

  • 1 Pages

    Microbiology-list of bacteria

    Palmer Chiropractic, MICRO 101

    Excerpt: ... ive anaerobe Bacillus subtilis gram +; makes spores Bacillus mycoides coiled-shaped "to be like fungi" Corynebacterium diphtheriae found in throats; diphtheroid toxin Corynebacterium xerosis on dry skin Propionibacterium acnes Helicobacter pylori rigid cell walls; one-several coils; spirillum Treponema pallidum spirochete; syphillis Borrelia burgdorferi spirochete; lyme disease Leptospira interogans spirochete; UTI Streptococcus mutans normal flora in mouth; capsule if sucrose present; gram +, dental caries Clostridium botulinum drumstick spores Clostridium tetani tennis racket spores Mycobacterium tuberculosis obligate anaerobe; 18 hour generation time Campylobacter jejuni microaerophile; thermophile; capnophile Listeria monocytogenes psychotroph, mesophile Neisseria gonorrhoeae gram -; "velcro" with fimbriae Brucella abortus - capnophile Streptococcus lactis acid-loving; ferments lactose to curdle milk Lactobacillus acidophilus - acid-loving; ferments lactose to curdle mil ...