Documents about High Performance Liquid Chromatography
321sch
CSU Northridge, HCCHM 321
Excerpt: ... Gas Chromatography (Ch. 24) High Performance Liquid Chromatography (Ch. 25) High Performance Liquid Chromatography (Ch. 25) Exam 3 [Ch. 14, 15, 18, 21, 23-25] Ion-Exchange Chromatography (Ch. 26) EDTA Titrations (Ch. 12) Final Exam [comprehensive] May 15 ...
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Seminar Answers 11.17.08
San Jose State, CHEM 250
Excerpt: ... ixing, heating and cooling. 3. Note three advantages to using LOC technology. a. Low sample consumption and waste production. b. Low cost c. Amenable to automation. Seminar Speaker: Sai Dharmaraj "UPLC" 1. What is the pressure that a high 3. What is (are) the main performance liquid advantage(s) of UPLC over chromatography cannot exceed? HPLC? a. 15,000 psi a. Higher speed and b. 2,000 psi resolution c. 50,000 psi b. Higher Selectivity d. 6,000 psi c. Higher Sensitivity d. Lower size and cost 2. What is the major difference between the high performance liquid chromatography and the ultra- high performance liquid chromatography ? a. Column size/particle size b. The pump technology c. Both a and b d. Neither a nor b ...
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Lecture35-081121
Minnesota, CHEM 4101
Excerpt: ... Chem 4101 Fall 2008 Lecture 35 Nov 20 High Performance Liquid Chromatography (HPLC) 1- What is HPLC? 2- Instrumentation -Chapter 27 Chem 4101 Fall 2008 Lecture 35 Nov 20 Instrumentation Figure 28-3, Sections 28A-C Chem 4101 Fall 2008 Lecture 35 Nov 20 Reciprocating pumps Figure 28-5, Sections 28-C Chem 4101 Fall 2008 Lecture 35 Nov 20 Detectors Section 28C Chem 4101 Fall 2008 Lecture 35 Nov 20 Absorbance detector Figure 28-8, Section 28C Chem 4101 Fall 2008 Lecture 35 Nov 20 Redox Potentials HPLC Analytes Figures 28-11, Sections 28C Chem 4101 Fall 2008 HPLC-MS Lecture 35 Nov 20 Figure 28-13, Sections 28C Chem 4101 Fall 2008 Lecture 35 Nov 20 Summary 1. Key components in an HPLC instrument include pumps, solvent proportioning valves, guard column, analytical column, sample injector, and a detector. Extra-column broadening must be avoided. 2. ...
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Problem_Set_V_-_High-Performance_Liquid_Chromat...
Rutgers, PHARM.CHEM 306
Excerpt: ... Problem Set V High-Performance Liquid Chromatography 1. 2. In reversed phase chromatography, the solutes are nonpolar and more soluble in a nonpolar mobile phase. In normal phase chromatography, the solutes are polar and more soluble in a polar stat ...
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exp16
Buffalo State, CHE 403
Excerpt: ... EXPERIMENT 16. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY The purpose of this experiment is to identify the aromatic hydrocarbons which are present in an unknown mixture and to determine the composition of the mixture. The student will also become familiar with the use of an isocratic and gradient mode of HPLC instrument and elements of the method development. Instrument Start Up 1. Turn on valves at hydrogen and helium cylinders. 2. Turn on all modules of HPLC instrument. See also the HP 1050 liquid chromatograph tutorial (See Chromatography computer lab you did in Block 3). In this laboratory experiment you are going to separate the mixture of aromatic hydrocarbons for qualitative determination. I assume that you have already used the HP 1050 in the Analytical Chemistry class for the caffeine determination. A mixture of hydrocarbons will be provided to run on the HPLC chromatograph so that the tr values may be obtained. 1. Introduce the unknown mixture into position #10 of the autosampler (you may assign a dif ...
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lab5
Delaware, CHEM 333
Excerpt: ... CHEM 333: Lab Experiment 5: INTRODUCTION TO CHROMATOGRAPHY; Thin Layer and High Performance Liquid Chromatography : PRE-LAB ASSIGNMENT! : The purpose of the first part of todays experiment is to determine the caffeine content of a beverage sample. Here is your chance to get involved and find out how much caffeine is in that stuff you drink. Which soda has the most caffeine? Red Bull really have more caffeine of normal cola? Tea have more caffeine than coffee? Which of your favorite coffee shops makes the most potent cup? Now is your chance to answer these questions and more that have plagued student-kind for centuries! (After all this information might come in handy as you study for your finals!) YOU ARE ALL TO BRING SAMPLE OF THE CAFINATED BEVERAGE OF YOUR CHOICE TO THIS WEEKS LAB. Pre-lab Reading Chapters 7, 8 and 9 This lab can actually be kind of fun (in a geeky sort of way). The techniques you will use this week are analyticaland you will be able to make actual determinations of caffeine ...
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HPLC
Central Washington University, CHEM 452
Excerpt: ... vti_encoding:SR|utf8-nl vti_timelastmodified:TR|27 Jan 2005 20:30:20 -0000 vti_extenderversion:SR|6.0.2.6353 vti_backlinkinfo:VX|Chem452w05/LabProcedures.htm vti_author:SR|PC7\JohanseA vti_modifiedby:SR|PC7\JohanseA vti_timecreated:TR|27 Jan 2005 19:30:20 -0000 vti_cacheddtm:TX|27 Jan 2005 19:30:20 -0000 vti_filesize:IR|35840 vti_cachedtitle:SR| High Performance Liquid Chromatography (HPLC) vti_title:SR| High Performance Liquid Chromatography (HPLC) ...
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HPLC
Central Washington University, CHEM 452
Excerpt: ... vti_encoding:SR|utf8-nl vti_timelastmodified:TR|27 Jan 2005 20:30:20 -0000 vti_extenderversion:SR|6.0.2.6353 vti_backlinkinfo:VX|Chem452s06/LabProcedures06.htm Chem452w05/LabProcedures.htm vti_author:SR|PC7\JohanseA vti_modifiedby:SR|PC7\JohanseA vti_timecreated:TR|27 Jan 2005 19:30:20 -0000 vti_cacheddtm:TX|27 Jan 2005 19:30:20 -0000 vti_filesize:IR|35840 vti_cachedtitle:SR| High Performance Liquid Chromatography (HPLC) vti_title:SR| High Performance Liquid Chromatography (HPLC) ...
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4212_Lab_Syl_F08
Mich Tech, CH 4212
Excerpt: ... Chemistry 4212 Instrumental Analysis Laboratory Fall 2008 Course schedule: Laboratory: Section 1: TR, 9 12 ChemSci 408 Section 2: TR, 2 5 ChemSci 408 Lab Supervisor: D. J. Chesney, office 19-404C; phone 370-7405; email djchesne@mtu.edu Graduate Teaching Assistant: Jeremy Sandrik, office 19-713; email jssandri@mtu.edu Office hours: By arrangement (email works well) 4212 Web Page: http:/chemistry.mtu.edu/~djchesne/classes/ch4212/ Format: Seven instrumentation explorations. Variable points as indicated below; based on demonstrated competency and evaluated by GTA. Major project report due Week 14. ACS format. Your instructor will be evaluating both the content AND the writing. 200 points. Total of 600 points scaled to 500 at the end of the course (to match lecture point total). Explorations: HPLC ( High Performance Liquid Chromatography ); four lab periods; 2 x 50 pts Gas Chromatography; three lab periods; 75 pts UV-Vis Spectroscopy; one lab period; 25 pts IR Spectroscopy; one l ...
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intro
Toledo, CHM 410
Excerpt: ... ANALYTICAL ENVIRONMENTAL CHEMISTRY IES 1410F/CHM 410F Autumn, 2007 Lecture Schedule Lecture, T,R 4-5, Rm158 LM Scott A. Mabury Office hours T & R 5 to 5:45; smabury@chem.utoronto.ca http:/www.chem.toronto.edu/analest/ Date Sept Lecture Chapter 11 T Analytical Environmental Chemistry ~ Intro 1 13 R Env Sampling/Data Handling/Problem Solving Appendix 1; 5 18 T Env Sampling/Data Handling/Problem Solving continued 20 R Sample Preparation 25 T Sample Preparation 27 R Separation Theory Oct 2 4 9 11 16 18 23 25 Nov 30 1 6 8 13 15 20 22 27 Dec T R T R T R T R T R T R T R T R T Separation Theory Gas Chromatography (DeSilva) GC Detectors and Applications (DeSilva) High Performance Liquid Chromatography (Butt) HPLC: Detectors and Applications (Butt) HPLC: Detectors and Applications HPLC: Detectors and Applications ImmunoAssay (D'eon) Midterm Examination Atomic Spectrophotometry Atomic Spectrophotometry Mass Spec Intro No Lecture (tentative) Mass Spectrometry: Ionization & Applications Mass Spectrometry: Ionization & ...
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Harbison
Truman State, CHEM 345
Excerpt: ... Histidine 454 Plays an Important Role in the Polymerization of Human Glutamate Dehydrogenase Nick W. Harbison Chemistry 345 19 October 2003 Human glutamate dehydrogenase (HGDH) is an important protein that daminates L-glutamate to 2-oxoglutarate. Allosteric regulation of HGDH can lead to hyperinsulinism/hyperammonemia syndrome in small children. To better understand the protein many studies are being done to determine how it maintains its quaternary structure. Through cassette mutagenesis, mutations at K333, K337, K344, K346, S445, G446 and H454 were studied. No effects on protein stability were shown in the mutant forms. KM data showed an increase in substrate affinity for the H454 mutant but the efficiency decreased. High performance liquid chromatography provided evidence that H454 plays a key role in formation of the quaternary structure of HDGH. Bibliography Lee, E.Y, Huh. J.W, Yang, S.J., Choi, S.Y., Cho, S.W., and Choi, H.J. FEBS Lett. 2003, 540, 163-166. Michaelidis, T.M., Tzimagiorgis, G., Mos ...
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lecture_seq
UWO, INSTRUCT 272
Excerpt: ... CHEMISTRY 272F - 2006-2007 "ANALYTICAL CHEMISTRY" IMPORTANT GENERAL INFORMATION CHEMISTRY 272 PRELIMINARY LECTURE SEQUENCE 2006-2007 By Dr. Zhifeng Ding 1. Chemistry 272 approximate number of lectures: 1 lecture - Operation of the course - The firs ...
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02ch1
Virginia Tech, LIB 112399
Excerpt: ... vestigate the effect of applied compressive strain, in a high-pressure steam environment, on the strength and stiffness of compressed wood. Samples compressed at specific moisture content, temperature, and pressure were tested in tension parallel to grain. SEM microscopy was employed for observing changes in cellular structure. Chemical composition of wood samples before and after desorption experiments was determined by acid hydrolysis followed by high performance liquid chromatography (HPLC). 1.2 TECHNICAL OBJECTIVES The objectives of this research were: 1) To investigate the effect of densification on ultimate tensile strength and stiffness of two wood species. 2) 3) To determine the effect of densification on the changes in wood structure. To determine the effect of densification on the chemical changes of wood. 1.3 RATIONALE AND SIGNIFICANCE In a short-term the results of this research will provide information for better understanding of modifications that occur during wood compression. This will help ...
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322lmtocold
CSU Northridge, HCCHM 003
Excerpt: ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Cyclic Voltammetry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Graphite-Furnace Atomic Absorption Spectrophotometry . . . . . . . . . . . . . . . . . . . . . . . . . . 24 Fluorescence Spectrophotometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Gas Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Gas Chromatography - Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Inductively Coupled Plasma - Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 High Performance Liquid Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 Ion Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 ...
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22lmtoc
CSU Northridge, HCCHM 003
Excerpt: ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Cyclic Voltammetry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Graphite-Furnace Atomic Absorption Spectrophotometry . . . . . . . . . . . . . . . . . . . . . . . . . . 24 Fluorescence Spectrophotometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Gas Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Gas Chromatography - Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 High Performance Liquid Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 Ion Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 X-Ray Diffraction Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ...
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322lmtoc
CSU Northridge, HCCHM 003
Excerpt: ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Spectrophotometric Determination of Components with Overlapping Absorption Spectra . 19 Graphite-Furnace Atomic Absorption Spectrophotometry . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Fluorescence Spectrophotometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 Gas Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 Gas Chromatography - Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 Inductively Coupled Plasma - Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 High Performance Liquid Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Ion Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 Ca ...
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NuvalaChE5
USF, SYMP 2006
Excerpt: ... Development of Biodegradable Microspheres to Release Anti-Inflammatory Drugs around Biosensor Nuvala Fomban1, Yvonne Moussy2, and Francis Moussy1 1Chemical Engineering and 2Mechanical Engineering, E-mail: nfomban@mail.usf.edu REU 2006 Poster N o. ChE.5 Objectives and Introduction To develop and optimize a local drug delivery system (i.e. microspheres) to continuously release an anti-inflammatory drug (dexamethasone) over 1 month. These dexamethasone loaded microspheres will be used to prevent inflammation and fibrosis of implanted glucose sensors. Materials and Methods Materials Poly(lactide-co-glycolide) PLGA 50:50 with ave.mol.wt.= 40-73kDa 2 g polyvinyl alcohol (PVA), Dexamethasone, (Sigma Chemicals) methylene chloride, methanol, acetonitrile, and isopropanol from (Fisher scientific) in High Performance Liquid Chromatography grade, and distilled water. Caution: Dichloromethane is extremely toxic. Method Development of Micropheres takes 3-4 days. The steps include: Dissolution of soli ...
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Harris23
Concordia Moorhead, CHEM 232
Excerpt: ... Chapter 23 Introduction to Analytical Separations I. II. III. IV. V. Solvent Extraction Principles of Chromatography Language of Chromatography Gas Chromatography (GC) High Performance Liquid Chromatography (HPLC) Capillary Electrophoresis (CE) "The analytical instrument market is dominated by chromatography. LC is the biggest and one of the fastest growing areas, with worldwide annual revenues estimated at $2.2 billion. Coming in second is GC at $1 billion." - Analytical Chemistry 4/1/99, pg 271A VI. I. Solvent Extraction Definition separation based on different affinities of a solute for immiscible liquid phase I. Solvent Extraction (cont.) Ex: a) What volume of CHCl3 is needed to extract 99.5% of a solute from 100 mL of H2O if K = 610? b) What would be the total volume of CHCl3 needed to extract 99.5% of the solute in 4 equal extractions? KEY: It is more efficient to do several small extractions than one large extraction! 1 II. Principles of Chromatography Key Principle Chromatography is a sepa ...
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Chapter7-PrimarySequence
Waterloo, CHEM 233
Excerpt: ... Chemistry 233 Fundamentals of Biochemistry Primary Sequence Determination Covalent Structures of Proteins Sequence determination techniques: Composition (how many Asp, Ala, etc) N-terminal and C-terminal reagents cleavage of peptides (smaller fragments are easier to sequence) sequencing: Edman method or mass spectrometry We are here Proteins come in all sorts of sizes and shapes What methods can we use on all proteins for sequence determination? Primary structure (sequence) of bovine insulin Composition of a Protein Gives pretty much all the amino acids in the protein, but no sequence information! The steps in the procedure: i) hydrolyze all amide bonds with 6 N HCl at 100-110oC for 12-36 hrs in sealed tube ii) run the mixture on an ion exchange column (usually cation exchange at low pH) or reverse-phase (C18-hydrophobic) HPLC ( high performance liquid chromatography ) amino acids are derivatized for visualization integrate peaks to get relative numbers of each amino acid separation of an amino acid ...
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