Documents about Transmission Electron Microscopy
NANO510_F08
George Mason, NANO 510
Excerpt: ... le. Interactions of electrons, ions and photons with materials. Scanning Electron Microscopy, Auger Electron Spectroscopy, Transmission Electron Microscopy , Ballistic Electron Microscopy. Scanning Probe Microscopy, Scanning Tunneling Microscopy, Atomic Force Microscopy. Near-field Scanning Optical Microscopy, Fluorescence Resonance Energy Transfer, Surface Plasmon Resonance. Ion beam techniques: Focused Ion Beam, Secondary Ion Mass Spectroscopy Imaging, Ion Channeling. Relevance of different characterization techniques to study problems in physical and biological sciences. Lecture notes will be the primary source of course materials. Suggested references: I. II. I. Brodie and J.J. Muray, The Physics of Micro-nanofabrication (Plenum, New York). J. Orloff, M. Utlaut, and L. Swanson, High Resolution Focused Ion Beams: FIB and Its Applications (Kluwer Academic/Plenum, New York, 2003). ...
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exam3studyguide
Wisconsin, ENGR 310
Excerpt: ... - chromatography Chapter 4 - transmission electron microscopy - scanning electron microscopy - scanning tunneling microscopy - atomic force microscopy - Michelson interferometer Chapter 5 - blood "anatomy" - blood types - centrifuge - cytometry manual, coulter counter, flow cytometry, fluorescence activated cell sorting Chapter 6 - light microscopy - resolution vs. magnification - fluorescence microscopy - confocal microscopy Optical coherence tomography ...
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PH 4-552 Jiao Spring 08
Portland, PH 552
Excerpt: ... Physics 452/552 Transmission Electron Microscopy Spring Quarter 2008 Credits: 4 credits 6 hours per week for 10 weeks (3 hours lecture, 3 hours lab per week) Instructor: Prof. Jun Jiao Office Hours: Thursday 14:00-16:00pm Office Location: SB 2, room 376 Tel. (503) 725-4228; e-mail: jiaoj@pdx.edu Required Textbook: Transmission Electron Microscopy by David B. Williams and C. Barry Carter. Lectures: Friday 9:00 11:50am, SB2, room 103. Labs: Friday 14:00 17:00pm, SB1, room 19 (basement). Course Description: The course is designed to introduce the theoretical and practical concepts of transmission electron microscopy (TEM), and to provide hands on opportunities for students to learn how to operate the state-of-the-art TEM and its attached analytical accessories. Instrument: Transmission electron microscope (TEM) used in this class is a JEOL 100CX TEM. Students will also learn the energy dispersive x-ray spectroscopy (EDX) from our state-of-the-art Tecnai F-20 high resolution TEM equipped with an ...
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SAD_workshop
Allan Hancock College, PAGE 115154
Excerpt: ... miliar with the basic principles and applications of electron diffraction in the TEM before attending the course. Many TEM-related books including electron diffraction information can be found in the UWA library. The following are recommended Transmission Electron Microscopy (1996), D.B. Williams and C.B. Carter, Plenum Press (one volume of the 4-volume set covers diffraction) Electron beam analysis of materials (1994) M.H. Loretto, Chapman and Hall Electron microscopy and analysis (2001) Peter J. Goodhew, John Humphreys, Richard Beanland, Taylor and Francis Transmission electron microscopy and diffractometry of materials (2002) Brent Fultz and James Howe, Springer Introduction to conventional transmission electron microscopy (2003) Marc De Graef, Cambridge University Press The library also houses a set of DVD recordings of microscopy workshops run by the Microscopy Society of America. RELATED TRAINING OPPORTUNITIES: Other TEM-related workshops currently include TEM training course (for basic ...
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NANO703_Fall08_Sched
SDSMT, NANO 703
Excerpt: ... esolution Imaging TEM: Image Simulation TEM: Image Processing TEM: Other Imaging Techniques SEM: Operation & Capabilities SEM: Beam Specimen Interactions SEM: Image Formation and Interpretation X-Ray Microanalysis Qualitative & Quantitative EDX (cont'd.) XRD: Properties of X-Rays XRD: Diffraction Geometry XRD: Intensities of Diffracted Beams Ellipsometry (cont'd.) (cont'd.) Other Instrumentation Conclusion READING TEM: Ch. 1,2 TEM: Ch. 3-5 EXAMS 3 TEM: Ch. 6,8,9 4 TEM: Ch. 11-12 5 TEM: Ch. 13,14 6 TEM: Ch. 16-18 7 8 TEM: Ch. 19-21 Exam 1 TEM: Ch. 22-24 9 TEM: Ch. 25,27,28 10 TEM: Ch. 29-31 11 SEM: Ch. 1-5 12 TEM: Ch.33-36 (or SEM: Ch. 6-10) XRD: Ch. 1,3 Exam 2 XRD: Ch. 4 13 14 15 Final Exam Texts: TEM: Transmission Electron Microscopy , D. B. Williams & C. B. Carter, (Springer, 1996). SEM: Scanning Electron Microscopy and X-Ray Microanalysis, 3rd Ed., J. Goldstein et al. (Springer, 2003). XRD: Elements of X-Ray Diffraction, 3rd Ed., B. D. Cullity & S. R. Stock, (Prentice Hall, 2001). LA ...
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Lab1-A
Purdue, MSE 582
Excerpt: ... MSE 595T Basic Transmission Electron Microscopy Laboratory I TEM Construction and Controls Purpose The purpose of this lab is to acquaint beginners with general TEM construction and a particular instrument in this case the JEOL 2000FX. Please refer to the operation manual or your instructor for more information. You will also learn how to insert and remove specimens, under the guidance of your instructor. Know your microscope: Identify the parts of the TEM Students are required to familiarize themselves with the basic construction and controls of the TEM. Although this description is pertinent to the JEOL 2000FX microscope, it could be extended to any TEM, since the basic construction and operation is similar. Students are required to check and acquaint themselves with the controls listed on the following pages. Consult the operation manual and your instructor to understand the location and basic functions of each control. Report Requirements Lab reports are required individually, within seven days. Your ...
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6010.Fall04
Georgia Tech, MSE 6010
Excerpt: ... MATE 6110 Transmission Electron Microscopy Lecturers: Objective: Dr. Z.L. Wang, Regents' Professor of Materials Science and Engineering Tel: 894-8008, e-mail: zhong.wang@mse.gatech.edu To introduce the kinematical electron scattering theory, optics in TEM, diffraction contrast imaging of crystals containing defects, and dynamical electron diffraction effects. To introduce the concept of reciprocal space, basis for indexing electron diffraction patterns, and diffraction from twinned crystals. To introduce the theory, techniques and applications of highresolution transmission electron microscopy (HRTEM) in materials research. To describe chemical microanalysis using EDS Functional and Smart Materials - structural evolution and structure analysis by Z.L. Wang and Z.C. Kang (Plenum Press, 1997) Text: Reference book: Elastic and Inelastic Scattering in Electron Diffraction and Imaging by Z.L. Wang (Plenum Press, 1997) Home work Exams Grade 4 assignments Middle term and final 30% Home work 35% Middle term exam 35 ...
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class7
Lincoln U. CA, FACULTY 4250
Excerpt: ... rock Transmission Electron Microscopy (TEM) Sample sectioned very thin; often stained with electron-dense stain (such as osmium tetroxide) Relatively broad electron beam passes through the sample Magnetic lenses then magnify the image by refracting the beam, which then hits a phosphorescent screen TEM cross-section through a mitochondrion TEM cross-section through plant cells TEM cross-section through plant cells dividing Usually, a sample for TEM (such as this bacterium) is sectioned very thinly (about 60 nm) and stained with osmium or uranium salts, which are attracted to lipids and highly opaque to electrons. . . Different staining techniques bring out details of whole microorganisms under TEM, without sectioning. Compare these negatively stained whole bacteria. . . . . . with this shadowed bacterium, which has a lm of metal applied to the sample from an acute angle, giving an illusion of depth. Negatively stained bacteriophage under TEM Shadowed bacteriophage under TEM 3 ...
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SQ02_Lect_4_6
Cornell, BIO 2900
Excerpt: ... ster or Ether) ? Do they have ribosomes? If yes, what size? Archaea Bacteria Viruses 13) Write the name of the Protistan group (Fungi, Slime Mold, Algae, or Protozoa) next to the phrase that describes members of the group. _ filamentous organisms with chitinous cell walls. _ many are parasites with whip-like flagella. _ includes mushrooms, molds, and yeasts. _ carry out oxygenic photosynthesis. _ make fruiting bodies containing spores and feed by phagocytosis Part III. Microscopy 14) Compare and contrast light microscopy (LM) and transmission electron microscopy (TEM) LM TEM a) What is the radiation? b) What is used to bend the rays? c) What is the practical resolution limit? d) What is the usual magnification range? 15) What are the similarities and differences between a scanning electron microscope (SEM) and a confocal scanning laser microscope (CSLM)? (See BBOM 11e SEC. 4.24.3) BioMI 290 2/1/08 16) Label each image with the type of micro ...
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EFTEM_workshop
Allan Hancock College, PAGE 115155
Excerpt: ... Energy-Filtered Transmission Electron Microscopy (EFTEM) Workshop THE NEXT WORKSHOP WILL TAKE PLACE ON: Workshop will be organised when sufficient interest is registered (half day workshop). Register your interest with Dr Martin Saunders (martin@cmm.uwa.edu.au). WHO SHOULD ATTEND THIS COURSE? Researchers, PhD and Hons students in the physical or biological sciences with TEM experience intending to use energy-filtered TEM as part of their research project at the UWA Centre for Microscopy, Characterisation and Analysis (CMCA), including those wishing to: obtain element distribution images (including light elements such a B, C, N, O, etc.); improve the contrast in images from samples that show only weak contrast using conventional TEM imaging techniques (including unstained biological samples); improve image quality and contrast in images of thick samples. COURSE OUTLINE: The principles and applications of energy-filtered TEM and elemental mapping will be reviewed through an initial lecture. Practical tr ...
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Lecture4-2
University of Illinois, Urbana Champaign, GEO 432
Excerpt: ... Geol432 Mineralogy & Mineral Optics 4-2 Lecture 9/14/06 Analytical Methods in Mineral Science Objectives Get to know the names and acronyms of some commonly used analytical methods in modern mineral science. Get to know the basic principles behind these methods. Modern mineralogy takes advantage of new analytical methods developed in solid state physics, solid state chemistry, and material sciences. Well-established methods include optical microscopy, X-Ray Diffraction (XRD), scanning electron microscopy (SEM), electron microprobe analysis (EMPA), Raman and infrared spectroscopy, X-Ray Fluorescence (XRF) also known as X-ray Emission Spectroscopy (XES), Mssbauer spectroscopy, Transmission Electron Microscopy (TEM), and more recently, Atomic Force Microscopy (AFM), Secondary Ion Mass Spectrometry (SIMS). Some state-of-the-art techniques include Focused Ion Beam (FIB) probe, Nuclear Resonant Scattering (NRS), and Inelastic X-ray Scattering (IXS). In optical microscopy, a petrographic microscope is u ...
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Practice Midterm I B
UCLA, MCDB 165A
Excerpt: ... t: a) are found in hemidemosomes b) are receptors for selectins c) interact with extracellular proteins via RGD peptide domains d) bind intracellularly to Tailin via the subunit e) activate the MAPK pathway 21. Selectins are cell-adhesion molecules which recognize and bind to: a) RGD-containing peptides b) Glycoprotein carbohydrate groups c) Intermediate filaments d) Basal lamina e) Soluble growth factors 22. Your laboratory mentor has asked you to obtain information on the distribution and topography of the cell surface in two distinct cell types. What technique will you use to obtain this information? a) Scanning electron microscopy b) Transmission electron microscopy c) Confocal microscopy d) Contrast phase microscopy e) Freeze fracture and transmission electron microscopy 3 Name: ID#: 23. The composition and organization of the extracellular matrix conveys the physical characteristics of different tissues. In the case of the cornea, its transparency is a consequence of the: a) parallel organizati ...
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Bio notes 6
Cornell, BIO G 101
Excerpt: ... Zhaonan Liu Bio Lecture 6 Membrane Structure and Function 9/7/07 Red blood cell ghosts 1915 1915 Data: Membrane consists of phospholipids and proteins 1925 Data: Stable phospholipid bilayer can self assemble 1935 Data: Membrane is more wettable than a pure phospholipid bilayer Davson & Danielli propose sandwich model with more hydrophilic proteins on the outside 1950s Data: Transmission electron microscopy (TEM) reveals 3 layered sandwich Problems with Davson-Danielli model Data: Cholesterol is present in membranes of eukaryotes, but not in prokaryotes Data: Plasma membrane is 7-8 nm thick, 3 layers but mitochondrial membrane is only 5-6 nm thick and looks like a row of beads, not a sandwich Data: Protein composition differs in different membranes Most inconsistent data: membrane proteins are not very soluble in water and have hydrophobic regions Fluid Mosaic model SJ Singer & GL Nicholson, 1972 Prediction: Freeze fracture, transmission electron microscopy (TEM) should reveal proteins embedded in the me ...
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b
Laurentian, BIO 1010
Excerpt: ... ctron beams have wavelength much shorter than visible light. This helps the EM to have resolving power of about 2 nm. Most subcellular structures (organelles) can not be visualised by LM, cell ultrastructure is studied with the use of EM. TEM transmission electron microscopy TEM transmission electron microscope is similar to light microscope but instead transmits electrons (light in LM) through electromagnets (lenses in case of LM). The image can be focused either onto a screen or onto photographic film. The use mainly for the study of internal ultrastructure of cells. SEM scanning electron microscopy The electron beam scans the surface of the sample, which is coated with a thin film of gold. The beam excites electrons on the sample's surface, and the secondary electrons are collected and focused onto a screen. This results in the appearance of three-dimensional image. The use detailed study of the surface of the object. LM versus EM LM advantageous for the study of live cells, they are cheaper ...
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Practice exam 1
Harvard, MCB 54
Excerpt: ... f the world that we could not otherwise imagine. Figure 1 was obtained using transmission electron microscopy . Figure 2 was obtained using phase contrast microscopy. (1) (2) A) Explain the essential differences between scanning and transmission EM with respect to sample preparation and the resulting images. B) Provide one example of the advantages and one example of the disadvantages of using electron microscopy compared with phase contrast microscopy. 2. (10 pts) Ingested glucose must move from the intestinal lumen (relatively low glucose concentration) through the intestinal epithelium (relatively high glucose concentration) in order to eventually reach the bloodstream. It is known that ATP is required for this entire process to occur, but the glucose transporter does not contain an ATPase domain. Based on what you know from lecture, explain where the ATP is being utilized and how the cell harvests this energy to transport glucose into epithelial cells. Name _ ...
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referencelist
UCSD, CHEM 165
Excerpt: ... CHM 165, 265 /B IMM 162 /B GG N 262 RE FE RE NCE LIST S The lectures in-class as well as lecture notes and powerpoints posted on the Web provide a fairly complete introduction to the principles and practice of transmission electron microscopy and to image reconstruction, BUT they are NOT meant to be comprehensive. This REFERENCE LISTS document will help guide you to books and articles from which you can obtain additional, more detailed descriptions of specific topics. To maximize the value derived from this course, the lectures and lecture notes ought to be supplemented by some outside reading. References identified with the ' symbol are recommended starting points. Notes: This list is far from complete and is a work in progress: when possible, updates will be posted on at http:/cryoem.ucsd.edu. Check the BOOK LIST document for complete citations for textbooks like Watt, Wishnitzer, Meek, Agar, etc. INTRODUCTORY MATERIAL Watt (1985) Chap. 1, pp.1-19 (Chap. 1, pp.1-29 in 1997 edition) Wischnitzer (1981 ...
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referencelist
UCSD, CHEM 165
Excerpt: ... CHM 165, 265 /B IMM 162 /B GG N 262 RE FE RE NCE LIST S The lectures in-class as well as lecture notes and powerpoints posted on the Web provide a fairly complete introduction to the principles and practice of transmission electron microscopy and to image reconstruction, BUT they are NOT meant to be comprehensive. This REFERENCE LISTS document will help guide you to books and articles from which you can obtain additional, more detailed descriptions of specific topics. To maximize the value derived from this course, the lectures and lecture notes ought to be supplemented by some outside reading. References identified with the ' symbol are recommended starting points. Notes: This list is far from complete and is a work in progress: when possible, updates will be posted on at http:/cryoem.ucsd.edu. Check the BOOK LIST document for complete citations for textbooks like Watt, Wishnitzer, Meek, Agar, etc. INTRODUCTORY MATERIAL Watt (1985) Chap. 1, pp.1-19 (Chap. 1, pp.1-29 in 1997 edition) Wischnitzer (1981) ...
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referencelist
UCSD, CHEM 165
Excerpt: ... CHE M 16 5,26 5/B IM M 16 2 /BG G N 2 62 RE FE RE NCE LIST S The class lecture notes provide a fairly complete introduction to the principles and practice of transmission electron microscopy and to image reconstruction, BUT they are NOT meant to be comprehensive. This optional reading list will help guide you to books and articles from which you can obtain additional, more detailed descriptions of specific topics. To maximize the value derived from this course, it is most helpful to supplement the lectures and lecture notes with some outside reading. Those references identified with the ' symbol are recommended starting points. Notes: This list has several `weak spots' and is far from complete: when possible, updates will be posted on the Web site (http:/cryoem.ucsd.edu). Check the Book List for complete citations for textbooks like Watt, Wishnitzer, Meek, etc. INTRODUCTORY MATERIAL Watt (1985) Chap. 1, pp.1-19 (Chap. 1, pp.1-29 in 1997 edition) Wischnitzer (1981) Chap. 1-3, pp.1-37 (pp.1-37 in 1970 edi ...
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referencelist
UCSD, CHEM 219
Excerpt: ... CHE M 21 9C /B IMM 162 /B G GN 26 2 RE FE RE NCE LIST S The lecture notes provide a fairly complete introduction to the principles and practice of transmission electron microscopy and to image reconstruction, BUT they are NOT comprehensive. The following optional reading list is meant to guide you to other places to obtain additional and more detailed descriptions of specific topics. To maximize the value derived from this course, it is most helpful to supplement the lectures and lecture notes with some outside reading. Those references identified with the ' symbol are recommended starting points. Notes: This list has several `weak spots' and is far from complete: when possible, updates will be posted on the Web site (http:/cryoem.ucsd.edu). Check the Book List for full citations for textbooks like Watt, Wishnitzer, Meek, etc. INTRODUCTORY MATERIAL Watt (1985) Chap. 1, pp.1-19 (Chap. 1, pp.1-29 in 1997 edition) Wischnitzer (1981) Chap. 1-3, pp.1-37 (pp.1-37 in 1970 edition) Meek (1976) Chap. 1-2, pp.1- ...
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sk2kb@1
Maryville MO, LSWINDEX 08
Excerpt: ... Silencing Soybean Apyrase Reduces Nodulation Kim, Sung-Yong1; Govindarajulu, Manjula; Tanaka, Kiwamu; Taylor, Christopher; Stacey, Gary 1Division of Plant Sciences, University of Missouri, Columbia, Missouri Apyrase hydrolyzes NTP to NDP and NMP. It plays an important role in neurotransmission modulation and platelet aggregation in animals and in phosphate uptake and mobilization in plants. Previously, o ver-expression of the soybean apyrase, GS52, in Lotus japonicus was shown to enhance nodulation b y rhizobia and to increase the level of infection. In this study, expression of the GS52 apyrase was sile nced by RNAi following transformation of soybean roots using Agrobacterium rhizogenes. The GS52 silenced soybean roots showed a significant reduction in nodule numbers. Examination of those nodu les formed by transmission electron microscopy showed incomplete infection inside the nodule. Base d on these results, the GS52 apyrase appears to be essential for the early rhizobium infection process in soybean. FI ...
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AS Quiz01 2007-2 CH385
West Point, CH 385
Excerpt: ... ize and shape but prokaryotic cells do not b. Prokaryotic cells differ in size and shape but eukaryotic cells do not c. Both eukaryotic and prokaryotic cells differ in size and shape 7. If a cell biologist wants to study the surface of a cell he/she would likely us a. Light microscopy b. Transmission electron microscopy c. Scanning electron microscopy 8. (4) What is one specific way in which prokaryotes differ from eukaryotes? Do not state the presence or absence of a nucleus. Multiple answers 9. (5) What is the current theory describing why prokaryotic cells are much smaller than eukaryotic cells? They are limited by the rate of diffusion. Extra credit (3 points) Name _ CH385 Introduction to Cell Biology Quiz #1 (30 points) Describe one aspect of Darwinian natural selection. ...
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040206seminar
TN Tech, CHEM 6910
Excerpt: ... later convert to other storage compounds (lactate polymers), 2. Polyphosphate accumulating organisms (convert lactate to polyhydroxy acids with a consumption of polyphosphate.) Morphological characteristics of microorganisms were determined by transmission electron microscopy and scanning electron microscopy. These studies demonstrate the exact mechanism for conversion of glucose into polyhydroxy acids with removal of phosphorus from wastewater. Friday Foster Hall February 06, 2004 2.00 pm ...
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ref_em
Purdue, BIOL 695
Excerpt: ... BIO 695M REFERENCES Electron microscopy SPRING 1999 Though the lecture notes provide a fairly complete introduction to the principles and practice of transmission electron microscopy , they are NOT comprehensive. I will hand out (starting with this page) an extensive set of optional reading lists. These will guide you to other references so you can obtain additional and more detailed descriptions of appropriate topics. To maximize the value derived from this course, it is most helpful to supplement the lectures and lecture notes with at least some outside reading. Those references identified with the ' symbol are recommended starting points. INTRODUCTORY MATERIAL Watt (1985) Chap. 1, pp.1-19 Wischnitzer (1981) Chap. 1-3, pp.1-37 (pp.1-37 in 1970 edition) Meek (1976) Chap. 1-2, pp.1-59 (pp.1-65 in 1970 edition) Agar (1974) Chap. 1, pp.1-37 Slayter (1970) Chap. 16, pp.341-350 Sjostrand (1967) Chap. 2-3, pp.15-128 Hall (1966) Chap. 1-2, pp.1-43 HISTORY OF THE ELECTRON MICROSCOPE Wischnitzer (1981) Chap. ...
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