• 14 Pages practice Final Exam
    Practice Final Exam

    School: UCLA

    Course: LS 3

    LS3 Final Exam INSTR: L. Johnson Thursday, Dec. 12, 2002 NAME:_ (last) (first) I.D. #_ INSTRUCTIONS 1. This exam is 14 pages long. Before starting make sure you have all the pages. 2. Be sure to write clearly. Only legible answers will be graded. PA

  • 12 Pages LS3%20Midterm%20I_TA_ans_09W
    LS3%20Midterm%20I_TA_ans_09W

    School: UCLA

    Course: LS 3

    LS3 Midterm I (120 pts) Feb 5, 2009 Instr. P. O'Lague NAME_ (Last) (First) I.D.#_ Instructions: 1. This exam has 12 pages including the Data sheet. Be sure you have all pages. 2. WRITE YOUR NAME ON EVERY PAGE. 3. For TRUE/FALSE (2pts) and MULTIPLE CH

  • 15 Pages LEC13
    LEC13

    School: UCLA

    Course: LS 3

    Midterm I Average: Hi Lo A's: B's: C's: 65 96 13 80-100 60-79 40-59 Regrades Check exam with key online One week to turn in exam for regrades Write on front of exam which problem(s) you want regraded Turn it in to me. DNA Polymerase III Holoenzyme

  • 19 Pages LEC12
    LEC12

    School: UCLA

    Course: LS 3

    DNA Replication DNA Replication (S phase) Cell Division (Mitosis) Mechanism proposed by Watson & Crick Old strand New strand Old strand Models for DNA replication (Meselson-Stahl, 1958) Conservative Semi-conservative How can one distinguish

  • 20 Pages LEC11
    LEC11

    School: UCLA

    Course: LS 3

    Announcements Midterm: 5-7 pm Thursday Broad 2160E TAs: Janet, Diana, Ronika, Shawn, Gabriela Haines 39 TAs: Danny, Rachel, Jessica, Jennifer, Ana Marie MS4000A TAs: Lynn, Lauren, Courtney, Allen Translation: in detail Ribosomal RNAs Seco

  • 19 Pages LEC10
    LEC10

    School: UCLA

    Course: LS 3

    Announcements Quiz 2 Two attempts Due by Monday noon Wednesday Review in class Midterm 5-7 pm Thursday All material through Monday lecture Central Dogma of Molecular Biology Gene expression Gene Expression Transcription: DNA -> RNA sa

  • 3 Pages LEC09.ppt [Compatibility Mode]
    LEC09.ppt [Compatibility Mode]

    School: UCLA

    Course: LS 3

    Announcements ! Review next week in class Wed class! Midterm 5-7 pm- Thursday ! Quiz 2: everyone will get 0.5 pts additional ! Quiz 3: this weekend lac operon is also under positive control Observation: Lactose media: !-gal activity high Glucose med

  • 3 Pages LEC08
    LEC08

    School: UCLA

    Course: LS 3

    10/13/2008 Announcements Lab 5 this week Quiz 2 Answer posted Wed. Prokaryotic Gene Regulation 1 10/13/2008 Promoter sequence determines first level of regulation Weak promoters have poor consensus sequences and initiate transcription infrequ

  • 19 Pages LEC07.PPT [Compatibility Mode]
    LEC07.PPT [Compatibility Mode]

    School: UCLA

    Course: LS 3

    10/10/200 Announcements ! Next week Lab 5 ! galactosidase 5- !induction ! Quiz 2 " " " You will have two attempts Do not use your back button on the y browser (it may crash) Multiple choice or T/F Transcription: synthesis of RNA from a DNA templ

  • 19 Pages LEC06.PPT [Compatibility Mode]
    LEC06.PPT [Compatibility Mode]

    School: UCLA

    Course: LS 3

    !Enzymes Structure/function /f Regulation !Molecular Chaperones !Antibodies Structure Uses in Molecular Biology Protein Function ! Function of a protein is determined by it's 3D structure ! Changing as little as a single amino acid can be detr

  • 3 Pages LEC14
    LEC14

    School: UCLA

    Course: LS 3

    Announcements If you haven't picked up your exam, your TA should have it. One week for regrades Quiz 4: Due Monday noon Mutations: change in DNA sequence Positive: Provide genetic variation evolution drive Negative: lead to heritable genetic disord

  • 22 Pages lec15-----
    Lec15-----

    School: UCLA

    Course: LS 3

    Announcements Quiz 4: due today Regrades: due Wed. Recombinant DNA technology and Gene cloning Early studies of lambda led the way to recombinant DNA technology Cos sites are 12 bases long 100,000 BP 5'pGGGCGGCGACCTC G G CCCCGCCGCTGGAp 3' GGGGCGG

  • 19 Pages LEC16
    LEC16

    School: UCLA

    Course: LS 3

    11/6/2008 Announcements Last day for regrades You may pick up regraded exams next week in the LS Core office Vectors Classic vectors pBR322 B First artificial plasmid Named after scientist Bolivar and Rodriguez Derived from 2 naturally occur

  • 11 Pages LEC24
    LEC24

    School: UCLA

    Course: LS 3

    12/3/2008 Announcements Regrades due by Friday Office Hours: Today 2-3pm; Monday 1-2 pm Today, Monday, Final Exam: Tuesday 6:30-9:30 Moore 100: Janet, Diana, Ronika, Shawn, Gabriela, Allen La Kretz 110: Danny, Rachel, Jessica, Jennifer, Ana Mari

  • 17 Pages LEC23
    LEC23

    School: UCLA

    Course: LS 3

    Studying Tips for Final: - Look at all lectures and compare Eukaryote VS Prokaryote - RNA VS DNA - When studying try to get a bigger picture of the whole thing. - Exam: 200 pts Lecture & 20 pts Lab(multiple choice) 12/1/2008 Epigenetics Changes in

  • 16 Pages LEC22
    LEC22

    School: UCLA

    Course: LS 3

    Announcements Midterms will be handed back next Monday in class or in discussion section TAs will hand back exams to their own students You will have until Friday, Dec. 5th to turn it in for regrade Please do not turn in a regrade without checking th

  • 16 Pages LEC21
    LEC21

    School: UCLA

    Course: LS 3

    11/18/2008 Announcements Midterm II: next Thursday, Nov. 20th B Broad 2160E: Janet, Diana, Ronika, Shawn, d 16 E D k h Gabriela, Allen Haines 39: Danny, Rachel, Jessica, Jennifer, Ana Marie MS4000A: Lynn, Lauren, Courtney In class review: Wedne

  • 20 Pages LEC20
    LEC20

    School: UCLA

    Course: LS 3

    11/16/2008 Announcements Last Quiz this weekend Midt Midterm II next Th d II: t Thursday, N Nov. 20th In class review: Wednesday Friday: no lecture Broad 2160E: Janet, Diana, Ronika, Shawn, Gabriela, Allen Haines 39: Danny, Rachel, Jessica,

  • 15 Pages LEC19
    LEC19

    School: UCLA

    Course: LS 3

    11/12/2008 Overall structure very similar to prokaryotic RNA polymerase Assembly of Active Transcription Complexes Prokaryotes: y RNA polymerase holoenzyme binds directly to the promoter. Eukaryotes: General Transcription Factors (TFIID, TFIIA, TF

  • 15 Pages LEC18
    LEC18

    School: UCLA

    Course: LS 3

    11/10/2008 Announcements Regraded exams can be picked up in the core office ffi LS3 labs New policy: drop lowest lab score Do not need to make up lab if miss one If you have a midterm conflict with lab this y week, you can drop this weeks lab

  • 28 Pages LEC17
    LEC17

    School: UCLA

    Course: LS 3

    11/7/2008 Announcements Quiz this weekend: after 5 pm today Regrades will be finished this weekend Pick up in core office Monday (LS2305) Sanger or Dideoxy Sequencing 1. DNA to be sequenced is first denatured 2. Oligonucleotide of known sequence

  • 3 Pages LEC05a.ppt [Compatibility Mode]
    LEC05a.ppt [Compatibility Mode]

    School: UCLA

    Course: LS 3

    10/6/200 Announcements Due to technical difficulties some students encountered, thi weeks quiz t d t t d this k i will not count Quiz Oct 10 Two attempts No multiple answer or fill in the blank By Friday, this past quiz and answers will be pos

  • 19 Pages LEC04a.ppt [Compatibility Mode]
    LEC04a.ppt [Compatibility Mode]

    School: UCLA

    Course: LS 3

    10/6/200 RNA differs from DNA in four basic respects Ribose instead of deoxyribose in the backbone (less stable in presence of alkali) Uracil instead thymine (5-methyluracil) ) Single stranded Secondary structure OH instead of H U instead of T

  • 19 Pages LEC03a.ppt [Compatibility Mode]
    LEC03a.ppt [Compatibility Mode]

    School: UCLA

    Course: LS 3

    Figure 2.10 Melting temperature: Temperature at which of DNA molecules are denatured Beyond the double helix Unusual DNA Secondary Structures Supercoiling of DNA can be described by Linking number, Twist and Writhe L=T+W L T W relaxed supercoil

  • 1 Page Final Review
    Final Review

    School: UCLA

    Course: LS 3

    Final Review RNA splicing hnRNA vs mRNA snRNPs- where they bind, what they do formation of spliceosome Splice sites (5', branch, 3') Two transesterification reactions Structure branch A Exonic splicing enhancers/SR proteins Alternative splicing Epige

  • 3 Pages quiz1
    Quiz1

    School: UCLA

    Course: LS 3

    1. Question Match the conclusions with the experiment below. Answer Match Question Items C. - A. This experiment showed that DNA alone could transform the non-lethal strain into a lethal strain. Answer Items A. Griffiths experiment D. - B. This exp

  • 2 Pages quiz 3
    Quiz 3

    School: UCLA

    Course: LS 3

    Quiz 3 1. Which of the following lactose analogs can be used as a substrate? (you may need to consult your lab manual as well as your notes) a. IPTG b. XG c. ONPG d. a and b e. b and c 2. Which of the following compounds can induce the lac operon? a.

  • 3 Pages LS3-Syllabus
    LS3-Syllabus

    School: UCLA

    Course: LS 3

    Date 9/26/08 9/29/08 10/1/08 10/3/08 Lecture Topic Experiments on Chemical 1 Nature of the Gene 2 Hershey Chase exp. Structure of DNA 3 4 Genome structure RNA structure Central Dogma of Molecular Biology Protein Structure, electrophoresis Enzymes, c

  • 1 Page Practice Qs for Midterm 2 Set #1
    Practice Qs For Midterm 2 Set #1

    School: UCLA

    Course: LS 3

    Practice problems for Midterm 2: *DNA replication*DNA repair*Cloning* 1) Suppose you were to run a PCR on normal DNA using dNTP's labeled with 15N. How would the DNA separate on a CsCl gradient after one cycle? How would the DNA separate on a CsCl g

  • 4 Pages Eukaryotic
    Eukaryotic

    School: UCLA

    Course: LS 3

    Transcription In Eukaryotes I. How's it different from prokaryotic (remember, PolII, sigma factor, -10 and -30 binding sites etc.): A. There's a nuclear membrane between the transcription machinery and the translation machinery- transcription and t

  • 3 Pages DNA
    DNA

    School: UCLA

    Course: LS 3

    Repair Mechanism Lesion Bypass When does it happen? During replication- have to keep going! WIll replicate through Thymine dimer When bases are chemically damaged (thymine dimer, methylation damage) When you need to correct mistakes the Pol made or

  • 21 Pages LS3lect11_Eucayotic%20_trans09W
    LS3lect11_Eucayotic%20_trans09W

    School: UCLA

    Course: LS 3

    Eucaryotic Translation As in prokaryotic translation, protein translation in eucaryotes consists of three phases. 1. Initiation 2. Elongation 3. Termination * * eIF4F is a complex of a cap binding protein, eIF4A and eIF4E Initiation of Eucaryotic

  • 14 Pages Practice Final Examkey
    Practice Final Examkey

    School: UCLA

    Course: LS 3

    LS3 Final Exam INSTR: L. Johnson Thursday, Dec. 12, 2002 NAME:_ (last) (first) I.D. #_ INSTRUCTIONS 1. This exam is 10 pages long. Before starting make sure you have all the pages. 2. Be sure to write clearly. Only legible answers will be graded. PA

  • 21 Pages RNAvsDNA
    RNAvsDNA

    School: UCLA

    Course: LS 3

    Patterns and principles of RNA structure RNA structure can be specific, stable and complex. (As a result, RNA mediates specific recognition and catalytic reactions.) Principles/ideas-RNAs contain characteristic 2 and 3 motifs Secondary structure-stem

  • 4 Pages Midterm 1 (pracitce)
    Midterm 1 (pracitce)

    School: UCLA

    Course: LS 3

    LS3 MIDTERM I Practice Exam (10 pts) 1. In Griffith's transformation experiment, he heat killed the S strain, mixed it with a small number of R strain cells and injected a mouse (please answer the following with one or two words). a) What happened to

  • 3 Pages LEC02A.PPT [Compatibility Mode]
    LEC02A.PPT [Compatibility Mode]

    School: UCLA

    Course: LS 3

    Last time: ! Griffith experiment and Avery et. al. experiments, ! Transforming agent is DNA ! Not accepted universally ! Did open scientific thought to the possibility that DNA may be the chemical associated with "genes" ! Stimulated experiments in

  • 21 Pages lec01a.ppt [Compatibility Mode]
    Lec01a.ppt [Compatibility Mode]

    School: UCLA

    Course: LS 3

    Course Materials ! Fundamental Molecular Biology " ! LS3 lab manual ! LS3 reader " Lizabeth Allison, 1st edition ! Web site " Course Reader Material, 1141 Westwood Blvd. http:/www.lsic.ucla.edu/ Discussion/Lab ! Week 1, 3, 5, 7, and 10 you will

  • 6 Pages mideterm 2 review
    Mideterm 2 Review

    School: UCLA

    Course: LS 3

    Answer for Midterm 2 review questions: What size is the target insert vector? 2+6+3=11kb What size is your target insert? 2kb Your boss want Your Favorite Gene to be inserted into a vector that will allow both selection and screening of e.coli coloni

  • 6 Pages Midterm 2 concepts
    Midterm 2 Concepts

    School: UCLA

    Course: LS 3

    Midterm 2 concepts: DNA REPLICATION Models for DNA Replication: 1. Conservative 2. Semi-Conservative Ways to distinguish between old and new DNA strand: 1. CsCl gradient to separate DNA with a different isotope of nitrogen (N14=light, N15=heavy) 2. B

  • 3 Pages mid II review
    Mid II Review

    School: UCLA

    Course: LS 3

    Midterm II review Replication Conservative vs semi-conservative Meselson-Stahl experiment Origin of replication, replication bubble bidirectional vs unidirectional RNA vs DNA synthesis Pol I, Pol III leading and lagging strand template

  • 8 Pages MIkey
    MIkey

    School: UCLA

    Course: LS 3

    LS3 MIDTERM I INSTR: L. JOHNSON NAME:_ (last) (first) I.D. #_ TA _ INSTRUCTIONS 1. This exam is 8 pages long. Before starting make sure you have all the pages. 2. WRITE YOUR NAME ON EVERY PAGE. 3. No credit will be given for an illegible answer. 4. W

  • 8 Pages MIkey-1
    MIkey-1

    School: UCLA

    Course: LS 3

    LS3 MIDTERM I INSTR: L. JOHNSON NAME:_ (last) (first) I.D. #_ TA _ INSTRUCTIONS 1. This exam is 8 pages long. Before starting make sure you have all the pages. 2. WRITE YOUR NAME ON EVERY PAGE. 3. No credit will be given for an illegible answer. 4. W

  • 3 Pages Midterm i review
    Midterm I Review

    School: UCLA

    Course: LS 3

    Midterm: You may write in pencil No cell phones, food, drink, notes on desk No bathroom stops Only 3 questions a piece Office hours WED: 2-3 pm in GEOLOGY 6704 Review ! ! ! ! Griffith Experiment o transformation Avery et al. Experiment Hershey-Chase

  • 10 Pages LS3%20Midterm%20IIans_3_09W
    LS3%20Midterm%20IIans_3_09W

    School: UCLA

    Course: LS 3

    LS3 Midterm II (130 pts) Feb 26, 2009 Instr. P. O'Lague NAME_ (Last) (First) I.D.# and TA_ Instructions: 1. This exam has 10 pages. Be sure you have all pages. 2. WRITE YOUR NAME ON EVERY PAGE (except data sheets). 3. For TRUE/FALSE (2pts) and MULTIP

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