Fertilizer and Chem-Indust Minerals
Fertilizer and ChemicalIndustrial Minerals
Limestone, Phosphate, Salt, Potash, Sulfur, Nitrogen Extremely important $65 Billion/year in total Nitrogen $37 Billion/year Other 5 @ $2-10 Billion/year (~ $28B total) N
Protein Purification and Expression
MCB 130L, Lecture 2
Why purify a protein?
To study its function To analyze its physical properties To determine its sequence For industrial or therapeutic applications
Protein expression in E. coli
Preparative Protein Chemistry
17 February 2009
But first, an update from the DNA sequencing world.
Now back to our protein purification lecture.
The Three "Eras" of Protein Purification
1. The "Classical" (Pre-Recombinant DNA) Er
Preparations for successful purification Examples of protein purification
IEX, GPC, IEX Immobilized metal affinity-IMAC Cell-free translation, density gradient
Characterization of purified proteins
Approaches indicated throug
Why bother with conventional protein purification methods?
Conventional Methods of Protein Purification
Rachel Britt email@example.com Biochemistry 660 September 10, 2008
Purity after tag not good enough Tag itself causes problems Cost of reagents
ANT 261: Science, Religion, and Society: Is the Truth Out There?
Spring Term, 2003: Monday, Wednesday, Friday 10:30 - 11:20 am, Chambers 1027 Instructor: Prof. Eriberto P. Lozada Jr. Office: Chambers B12 email: firstname.lastname@example.org tel. : 704-894-20
Recombinant DNA technology (III)
1. 2. 3. 4. Protein purification Protein analyses Protein expression in E. coli Protein expression in animal cells
1. Protein Purification
How to separate proteins from other molecules? How to separate one protei
Genetics 875 - November 30, 2006
Analysis of the yeast proteome
Goal: To perform a systematic analysis of protein complexes in Saccharomyces cerevisiae using tandem affinity purification and mass spectrometry
Why study protein interactions?
Introduction to The QIAexpress System
The expression, purification, detection, and assay of recombinant proteins has been made much more simple and powerful by the use of small affinity tags. The well-established QIAexpress Protein Expression and Pur
Reprinted from: Issues in new crops and new uses. 2007. J. Janick and A. Whipkey (eds.). ASHS Press, Alexandria, VA.
A Weed Control Program for Establishing Lesquerella
M.A. Foster, D.A. Dierig, and M.J. Wintermeyer Broad spectrum weed control is es
Emily Wade Environmental Engineering 12/11/07
Water pollution is a very common problem because there is a very small percentage of the earth's water that is fresh water. A pollutant of the water is defined as anything that decrea
Membrane Separation in the Dairy Industry
Carmen I. Moraru Cornell University
Principle of the separation process
The solution to be concentrated or fractionated
Also called retentate
The liquid passing through the mem