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1 nissl or tigroid substances coarse granules made up

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1.NISSL or TIGROID SUBSTANCESCoarse granules made up of RNA scattered inthe cytoplasm of nerve cellStained by H and E thionine, toluidine blue ormethylene blueDeep blue in toluidine blue against a colorlessbackground2.NEURONS, AXONS, NEUROFIBRILSBeilschowsky’s techniqueBlack on a grayinsh background3.ASTROCYTESMallory’s phosphotungstic acid hematoxylin(PTAH)Cajal’s gold sublimate methodBlack on a light brownish background4.NEUROGLIAAlkaline colloidal solution of an alkaline silver saltare reduced by neutral formalin especially in thepresence of light producing SILVER DEPOSIT ontissue elements5.MYELIN SHEATHMyelin is the fatty insulating sheath surroundingaxons made up of lipid cellsOsmium tetroxide methodBlack on a grayish backgroundConidia: deeprose to purpleElastic tissue/mucin: deepblue
Weigert-pal technique of staining normal myelinsheathBlue blackSudan Black method for rapid staining of myelin6.CEREBRAL LIPIDS:Thin Layer ChromatographyCHIEF SOLVENT USED FOR STAINS:1.Water2.Alcohola.Ethyl alcohol- any concentrationb.Methyl alcohol- absolute1.Aniline water2.PhenolPRECAUTIONS OBSERVED DURING STAININGAvoid stains on theskin.Failure of sections toremain on slide duringstaining.a.Dirty or greasy slideb.Too old albuminadhesivec.Incompletedehydrationd.Paraffin section notthoroughly spreadon slide whenmountedFailure of section to stain.a.Improper andinsufficiently ripenedHematoxylinb.Impurities in dye or inwater solventc.Deteriorated stains(loss of stainingproperty)-Precipitates in thestaining solution-Poor staining resultsFailure of staining dueto paraffin.Fixative ordecalcifying solutionthat has not beenthoroughly washedout and removed.Sections do notappear undermicroscope afterstaining:a.Water in theabsolute alcoholb.Moisture in thecover slipc.Too much eggalbumin on slide
d.Acid-alcoholdecolorizer notcompletelyremovede.Film of alkalinealcoholRESTAINING OLD, BLEACHED or FADED SECTIONSRemoval of coverslip by a dissecting needlea.Immersion in xylene for 24 hoursb.Heating gently the slide until mounting medium startsto bubbleOnce the coverslip has been removed, the slide isplaced in xylene for 30 minutes to remove remainingbalsamWash in running waterPlace in 0.5 POTASSIUM PERMANGANATE solution for 5-10 minutes and rinsed in tap waterImmerse in 5% OXALIC ACID for 5 minutes or until thesection is decolorizedWash in running water for 5 minutesStain using the desired techniqueTISSUE PIGMENTS AND DEPOSITSI. ARTEFACTS:highy colored, usually amorphous, granular,sometimes crystalline stain precipitates seen in sectiondue to faulty staining techniquesAcid Alcohol-most common solution in removingexcess stains in sections

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Term
Spring
Professor
N/A
Tags
HAc, TISSUE PHOTOGRAPHY

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