Forensics2 and gene expression (2).pptx - Forensics 2 Gene...

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Forensics 2 & Gene expre ssion
Gel electrophoresis Gel electrophoresis is a method for separation and analysis of macromolecules ( DNA , RNA and proteins ) and their fragment s, based on their size and charge. Plus end Minus end Agarose gel (0.8%)
-electrode (Black) +electrode (Red) Buffer level Too high GOOD ! Sample with bubble Too low 3 How to Load a Gel Loading PCR products on a gel
Gel electrophoresis-how does it work?
DNA ladder DNA ladder is a mixture of DNA fragments whose molecular sizes are already known DNA ladder serves a positive control in gel electrophoresis an d a good way to estimate your DNA sample sizes
Estimating DNA band with ladder 5k b 4k b This DNA band is approximately 5kb because it is nearly identical location compared to a 5kb DNA ladder band
Calculating DNA band sizes
Analyzing STR locus PCR products Lane 1- DNA ladder Lane 2- student 1

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