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Unformatted text preview: Name _________________________ TA ________________________ MCDB 1AL Lab Quiz 2 November 13 - 16 , 2006 Scantron Instructions: 1. Use a #2 pencil to complete the form. 2. Write your name and fill in the appropriate bubbles. 3. Write your perm. number in the ID number box and fill in the bubbles. 4. Fill in the entire rectangle of the answer you choose. If you erase, erase completely READ ALL QUESTIONS THOROUGHLY; IN ALL CASES, PICK THE BEST ANSWER 25 Questions - 1 point each 1. TPA 25 was amplified from cheek-cell DNA by PCR and the products run on an agarose gel just as you did in lab. In the gel shown here, samples were loaded onto the gel in the following order (from left to right) Lane 1: pGEM standards Lane 2: PCR product on individual A Lane 3: PCR product of individual B Lane 4: PCR product from individual C Lane 5: PCR product from individual D For which individuals can you definitively (i.e. unambiguously) say that TPA25 with the alu insert is not present in the entire genome. A. A (lane 2) B. B (lane 3) C. C (lane 4) D. D (lane 5) E. Cannot be unambiguously determined Show a gel with Lane 2 will have a "failed PCR reaction", 3 :++, 4 +-, 5 -- 2. What stage of mitosis is occurring in the cell indicated by the pointer? A. anaphase B. metaphase C. prometaphase D. telophase E. interphase Practical Question (Show students a slide of a cell during metaphase): 3. On the gel shown below, PCR products from an amplification of TPA25 were run just as you did in lab. You will notice the presence of a third band between the lowest and highest fragments. The reason for this band is most likely A. A new alu polymorphism in TPA25 B. The individual who's DNA was used as template is triploid C. Trisomy of chromosome 8 D. A mistake in the extension phase of PCR E. A mistake in the annealing phase of PCR Show a gel that has mispriming, i.e. multiple bands around the alu insert MCDB 1AL LQ2 Test Version: 0 Page: 1 4. Using the image of the gel and the graph given here, Estimate the size of Band X. The Rf values in the graph below were calculated exactly as you did in the lab. A. 400 (bp) B. 1300 (bp) C. 4000 (bp) D. 13,000 (bp) E. 40,000 (bp) Show a gel with standards x marks the 4,000 bp band, Rf 0.4 5. What would be the total volume in a tube if you were to pipette a solution into it once from each of the three pipettes? A. 466.5 l B. 480 l C. 601.5 L D. 615 L E. 750 L Practical Question (place a P1000, P200 and P20 pipette on the table, set to 450 L, 150 L and 15 L, respectively)....
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This note was uploaded on 04/09/2008 for the course MCDB 1AL taught by Professor Bush during the Fall '07 term at UCSB.
- Fall '07