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LABORATORY EXERCISE6BACTERIAL SMEARSWEEK7BACHELOR OF SCIENCE IN BIOLOGY:MICROBIOLOGY LABORATORYAt the end of this exercise, each student must be able to:oLearn and observe the proper procedures in preparing bacterial smears aseptically from abroth medium and a solid medium for the microscopic visualization of bacteriaoExplain the purpose of making bacterial smearsoKnow the different methods of fixation and understand the principle behind these methodsINSTRUCTIONSStudy the procedure in preparing bacterial smears from different culture mediaWatch the video tutorials on how to prepare bacterial smears.Accomplish Lab Report # 5INTRODUCTIONMost staining procedures such as Gram staining, AFB staining and endospore staining begin withpreparation of bacterial smear. A bacterial smear is a thin layer of the specimen placed on the slide.Bacterial smears from solid or broth media are performed to fix the bacteria to the slide so that thesample will not be washed off during staining.
As in other microbiology laboratory activities, it is important to practice aseptic technique during thepreparation of a bacterial smear. These measures are done to prevent the contamination of the cultureand other materials in the laboratory, and to ensure the safety of the people who are performing theprocedure.Some of these aseptic techniques include:Disinfecting the work area such as table tops before and after useMinimizing the time that cultures and growth media are open to the environment.Avoiding touching or breathing into the sterile culture media or the stock cultures.Sterilizing loops, needles, pipettes and other tools before and after they are usedRefraining from placing tube caps on the table top.Holding the tube caps in the hands while inoculatingFlaming the lip of test tube after the cap is removed.

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Term
Summer
Professor
N/A
Tags
Bacteria, Growth medium, Agar plate, inoculating loop

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