SBI4U_ Biological Macromolecules Lab.pdf - Avril Alvarez...

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Avril Alvarez Biological Macromolecules Lab Purpose: The purpose of this laboratory is to investigate the presence of macromolecules in everyday foods found in homes. Hypothesis: The substances containing starches will change colour and potentially create a precipitate due to the solutions added. The substances containing proteins will change in colour but will not create a precipitate or change in physical state once the testing solution is added. The substances containing lipids will show an oily, slippery substance once tested. Materials: - Apple juice - Albumin solution - Biuret solution - Distilled water - Iodine-potassium iodide solution - Milk - Pencil - Pipette(s) - Prepared starch solution - Test tubes - Vegetable oil -White paper Procedure: Test 1: Starches 1. Add 5mL of distilled water to test tube 1, 2, and 3. 2. Stir the starch solution then add 20 drops to test tube 2. 3. Stir the albumin solution then add 20 drops to test tube 3. 4. Add 4 drops of iodine-potassium iodide solution to all three test tubes. 5. Use white paper as a backdrop for observing physical changes. 6. Dispose of the solutions down the drain with water. 7. Clean and dry the test tubes. 1. Add 5mL of distilled water to test tube 2, 3, and 4. 2. Add 20 drops of apple juice to test tube 2. 3. Add 20 drops of vegetable oil to test tube 3. 4. Add 20 drops of milk to test tube 4. 5. Add 4 drops of iodine-potassium iodide solution to all the test tubes. 6. Use white paper as a backdrop and observe the test tubes for 30 seconds. 7. Dispose of the solutions down the drain with water. 8. Clean and dry the test tubes.

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