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Unformatted text preview: Homework Questions 1.) Draw a Ligand binding curve for a hemoglobin, and myoglobin. Label the axes, K D . Also draw curves for hemoglobin for a person who is at high altitudes, is exercising (has high blood lactate), and is a fetus. a. Explain what Θ is in a sentence: Bound subunits/ total subunits High altitudes will have a decreased affinity, shifting the graph to the right. Fetal Hb will have increased affinity, shifting the graph to the left. 2.) a. Define K D Dissociation constant [P][L] = [PL] b. If the K D is = [L], this means that: ½ of the protein is bound and ½ of the protein is unbound. 3.) What are the important structural similarities of 1 Hb subunit and Mb Alpha helices + loops His 93/F8 Globin Fold 4.) Fill in the following table T state R state Decreased Affinity Increased Affinity Oxygen unbound Oxygen bound Fe 2+ Fe 3+ Many AA interactions Less AA interactions His HC3 protonated to interact with Asp FG1 His HC3 not protonated 2,3 BPG binds and stabilizes BPG can’t bind...
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- Winter '07
- Biochemistry, Hemoglobin, high affinity, BPG, Affinity Oxygen