ExamI2008(2)-ans

ExamI2008(2)-ans - Payne, Biol 330 Exam #1 2008 Print Your...

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Payne, Biol 330 Exam #1 2008 Print Your Name: Your Laboratory Section:   DAY     TIME Directions : 1. Print Your Name and Section on every page of this exam  NOW . 2. Use a  PEN  to complete this exam or you will not necessarily be eligible for a  regrade. 3. Check to see that you have  6 pages . If you don t, raise your hand. 4. You  must  confine your answers to the spaces provided.  5.  Read  the questions! - Be as specific and as concise as you can. Use the  minimal  number of words necessary to completely answer the question. Use diagrams and  graphs if needed, but remember to label them! 6. You must hand in your exam by 9:50 AM. Good Luck. Scoring : Possible Pts. PAGE 2 /  24 PAGE 3 /  18 PAGE 4 /  24 PAGE 5 /  24 PAGE 6 /  10 TOTAL             /  100 I pledge on my honor that I have not given or received any unauthorized assistance on this examination 1
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Name ______________________ Lab Section day ______________ time_____________ 1) What limits the resolution of a light microscope? What is the approximate size range of the smallest structures that can be resolved? (4 pts) The wavelength of light (2pts) Range is 0.15 micrometers – 0.5 micrometers. (2pts(any number or numbers in this range would be ok. 1 pt off for “mm” not μ m or micrometers 2) Choose, from the list below (a-d), an appropriate microscopy method to solve the problems listed beneath the methods (e-h). (8 pts) a) polarization light microscopy b) DIC microscopy c) freeze-fracture electron microscopy d) video-enhanced light microscopy e) To view proteins imbedded in the outer leaflet of the plasma membrane f) To show the outline of a transparent membrane in a living, unstained cell. g) To view a large, highly ordered, “crystalline” structure within a cell h) To show the movement of a large organelle along a microtubule in a living cell Ans (e.g. a, f) __ a,g_ ________ ___b,f____ ____c,e_____ ___d,h____ 3) What microscope method might you use to measure the concentration of calcium ions within the cytoplasm of a cell? (2pts) Fluorescent microscopy (2pts) 4) You have purified a large quantity of protein X from mouse heart, but you do NOT know the
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This note was uploaded on 04/12/2008 for the course BSCI 330 taught by Professor Payne during the Spring '08 term at Maryland.

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ExamI2008(2)-ans - Payne, Biol 330 Exam #1 2008 Print Your...

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