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Special Topics on Immunologyand Serology
Presented by:Entrina, Irish AngelynEstares, Leslie LuzFuentes, Dygelyn AnnGallos, Amiel Bryan
Topics to be Discussed:IntroductionSpecimen Collection and HandlingPolymerace Chain Reaction (PCR)Reverse Transcription- Polymerase ChainReaction (RT-PCR)Electrophoresis
What is Immunology and Serology?Immunology is the study of the body's immune system and itsfunctions and disorders. Serology is the study of blood serum.Immunology and serology labs focus on:Finding antibodies. These are proteins made by a type of white blood cell inresponse to a foreign substance (antigen) in the body.Studying problems with the immune system. These include when the body'simmune system attacks its own tissues (autoimmune diseases). And when abody's immune system is underactive (immunodeficiency disorders).Checking organ, tissue, and fluid compatibility for transplantation. These testshelp reduce the risk of donor rejection.
Introdution
Polymerase ChainReactionPolymerase ChainReactionA simple in vitro chemical reaction thatpermitsthesynthesisofessentiallylimitless quantities of a targeted nucleicacid sequence.Accomplished through the action of adeoxyribonucleic acid (DNA) polymerasethat, under the right conditions, can copya strand of DNA.Steps:DenaturationAnnealingExtensionIdeally, after 20 cycles of PCR, a million-fold amplification is achieved, and after 30cycles, a billion-fold.
Polymerase ChainReactionPolymerase ChainReactionReverse transcriptasepolymerase chain reactionNested polymerase chainreactionMultiplex polymerasechain reactionEnd point quantitativepolymerase chain reactionReal time polymerasechain reactionDigital polymerase chainreactionRapid cycle polymerasechain reactionPCR TESTS
Reverse Transcription Polymerase ChainReaction (RT-PCR)

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Term
Spring
Professor
N/A
Tags
DNA, Reverse transcriptase, Reverse Transcription Polymerase Chain

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